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Becton Dickinson plasmid construction luria bertani lb media
Plasmid Construction Luria Bertani Lb Media, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid construction luria bertani lb media/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
plasmid construction luria bertani lb media - by Bioz Stars, 2020-04
86/100 stars

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Clone Assay:

Article Title: Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites
Article Snippet: Strains, media and plasmid construction Luria-Bertani (LB) media (10 g/l tryptone, 5 g/l yeast extract, 10 g/l NaCl) was obtained from BD and supplemented with 50 µg/ml chloramphenicol (Sigma-Aldrich). .. Synthetic 5′ UTRs were inserted by standard cloning techniques, utilizing either BamHI and SacI, or XbaI and SacI sites, depending on 5′ UTR length.

Plasmid Preparation:

Article Title: Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites
Article Snippet: .. Strains, media and plasmid construction Luria-Bertani (LB) media (10 g/l tryptone, 5 g/l yeast extract, 10 g/l NaCl) was obtained from BD and supplemented with 50 µg/ml chloramphenicol (Sigma-Aldrich). .. The expression system is derived from the pFTV1 plasmid (ColE1, CmR ) ( ) and encodes a σ70 constitutive promoter (BioBrick #J23100), a synthetic 5′ UTR and a codon-optimized mRFP1 fluorescent protein.

Expressing:

Article Title: Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites
Article Snippet: Strains, media and plasmid construction Luria-Bertani (LB) media (10 g/l tryptone, 5 g/l yeast extract, 10 g/l NaCl) was obtained from BD and supplemented with 50 µg/ml chloramphenicol (Sigma-Aldrich). .. The expression system is derived from the pFTV1 plasmid (ColE1, CmR ) ( ) and encodes a σ70 constitutive promoter (BioBrick #J23100), a synthetic 5′ UTR and a codon-optimized mRFP1 fluorescent protein.

Polymerase Chain Reaction:

Article Title: Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites
Article Snippet: Strains, media and plasmid construction Luria-Bertani (LB) media (10 g/l tryptone, 5 g/l yeast extract, 10 g/l NaCl) was obtained from BD and supplemented with 50 µg/ml chloramphenicol (Sigma-Aldrich). .. 5′ UTRs with desired sequences were created by either annealing of complementary oligonucleotides with overhangs, or by PCR assembly of oligonucleotides with corresponding restriction sites.

Derivative Assay:

Article Title: Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites
Article Snippet: Strains, media and plasmid construction Luria-Bertani (LB) media (10 g/l tryptone, 5 g/l yeast extract, 10 g/l NaCl) was obtained from BD and supplemented with 50 µg/ml chloramphenicol (Sigma-Aldrich). .. The expression system is derived from the pFTV1 plasmid (ColE1, CmR ) ( ) and encodes a σ70 constitutive promoter (BioBrick #J23100), a synthetic 5′ UTR and a codon-optimized mRFP1 fluorescent protein.

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    Becton Dickinson plasmid construction luria bertani lb media
    Plasmid Construction Luria Bertani Lb Media, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid construction luria bertani lb media/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid construction luria bertani lb media - by Bioz Stars, 2020-04
    86/100 stars
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