pikoreal 96 real time pcr system  (Thermo Fisher)


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    Name:
    PikoReal 96 Real-Time PCR System
    Description:

    Catalog Number:
    TCR0096
    Price:
    None
    Score:
    85
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    Thermo Fisher pikoreal 96 real time pcr system

    https://www.bioz.com/result/pikoreal 96 real time pcr system/product/Thermo Fisher
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    pikoreal 96 real time pcr system - by Bioz Stars, 2019-12
    99/100 stars

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    Amplification:

    Article Title: Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway
    Article Snippet: PCR was performed with 10 ng cDNA in a 20- µ l reaction system (2 µ l cDNA, 0.8 µ l 10 µ M forward primer, 0.8 µ l 10 µ M reverse primer, 10 µ l PCR mix buffer, and 6.4 µ l sterilized distilled water) using a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. The relative mRNA expression was normalized against the expression of β-actin and calculated using the 2−ΔΔCq method ( ).

    Article Title: Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway
    Article Snippet: PCR was performed with 10 ng cDNA in a 20-µ l reaction system (2 µ l cDNA, 0.8 µ l 10 µ M forward primer, 0.8 µ l 10 µ M reverse primer, 10 µ l PCR mix buffer, and 6.4 µ l sterilized distilled water) using a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. The relative mRNA expression was normalized against the expression of β-actin and calculated using the 2−ΔΔCq method ( ).

    Article Title: Glycinebetaine Biosynthesis in Response to Osmotic Stress Depends on Jasmonate Signaling in Watermelon Suspension Cells
    Article Snippet: Transcripts were amplified by PCR primers that were designed by Primer Premier 5 and listed in Supplementary Table . .. RT-qPCR was carried out using PikoReal 96 Real-Time PCR System (Thermo Fisher ScientificTM , MA, United States).

    Article Title: Binding of Hemagglutinin and Influenza Virus to a Peptide-Conjugated Lipid Membrane
    Article Snippet: The reaction was performed using a PikoReal Real-Time PCR system (TCR0096, Thermo Scientific). .. The optimized cycling conditions were as follows: RT reaction at 50°C for 2 min, 95°C for 15 min, initial denaturation at 95°C for 30 s, followed by 50 cycles of denaturation at 95°C for 5 s, primer annealing at 57°C for 20 s, and extension at 72°C for 10 s. Fluorescence was measured at the end of each cycle.

    Article Title: New functionally-enhanced soy proteins as food ingredients with anti-viral activity
    Article Snippet: The RNA was converted to full-length cDNA in the following reaction: 2.5 μl of DMPC (di-methyl pyrocarbonate) water, 5 μl of 5× First Strand buffer (Invitrogen), 0.5 μl of 10 mMdNTP mix (Amersham Biosciences), 2 μl of 50 mM UNi12 primer, 32 U of RNAguard (Amersham Biosciences), 200 U of MMLV reverse transcriptase (Invitrogen) and 5 μl RNA solution in total volume of 25 μl. .. Reactions were incubated at 42 °C for 60 min followed by inactivation of the enzyme at 95 °C for 5 min. PCR amplification with HA gene specific primers (Forward primer: 5′-AGCAAAAGCAGGGGA-3′, Reverse primer: 5′-AGTAGAAACAAGGGTGTT-3′) was performed on the PikoReal 96 Real Time PCR System (Thermo Scientific, USA) to amplify the product containing the fragment HA gene. .. The PCR mix contained: SybrGreen mix 10 μl (Thermo Scientific), 240 nM each of forward primer and reverse primer, ROX 0.04 μl and 5 μl cDNA for a 20 μl reaction volume.

    Synthesized:

    Article Title: Systematic pharmacological screens uncover novel pathways involved in cerebral cavernous malformations
    Article Snippet: Total RNA was extracted with TRIzol (Sigma) from pools of 7–15 embryos (48 hpf), and cDNA was synthesized with the RevertAid H Minus First Strand cDNA Synthesis kit (Thermo Fisher Scientific). .. Measurements were performed on a PikoReal 96 Real‐Time PCR System (Thermo Fisher Scientific).

    Article Title: Dendritic Homeostasis Disruption in a Novel Frontotemporal Dementia Mouse Model Expressing Cytoplasmic Fused in Sarcoma
    Article Snippet: 2.8 The cDNA was synthesized from 1 μg total RNA using the SuperscriptIII First-Strand Synthesis System (Life Technologies). .. Expression levels of glutamate receptor subunit were quantified using the DyNAmo ColorFlash SYBR Green qPCR Kit (Thermo Fisher Scientific) on a PikoReal 96 Real-Time PCR System (Thermo Fisher Scientific).

    Article Title: Heg1 and Ccm1/2 proteins control endocardial mechanosensitivity during zebrafish valvulogenesis
    Article Snippet: Total RNA was extracted with Trizol (Sigma) and Phase Lock Gel Heavy tubes (1.5 mL, Prime 5) and the corresponding cDNA was synthesized from total RNA with the RevertAid H Minus First Strand cDNA Synthesis kit (ThermoFisher Scientific). .. RT-qPCR experiments were performed as described ( ) using 18 ng cDNA per technical replicate and the KAPA Sybr Fast qPCR kit (Peqlab) on a PikoReal 96 Real-Time PCR System (ThermoFisher Scientific).

    Quantitative RT-PCR:

    Article Title: Systematic pharmacological screens uncover novel pathways involved in cerebral cavernous malformations
    Article Snippet: Paragraph title: Zebrafish RT‐qPCR ... Measurements were performed on a PikoReal 96 Real‐Time PCR System (Thermo Fisher Scientific).

    Article Title: Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway
    Article Snippet: Paragraph title: RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis ... PCR was performed with 10 ng cDNA in a 20- µ l reaction system (2 µ l cDNA, 0.8 µ l 10 µ M forward primer, 0.8 µ l 10 µ M reverse primer, 10 µ l PCR mix buffer, and 6.4 µ l sterilized distilled water) using a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

    Article Title: A Coding Variant in RARG Confers Susceptibility to Anthracycline-Induced Cardiotoxicity in Childhood Cancer
    Article Snippet: For real-time RT-PCR (qPCR) experiments RNA was purified from H9c2 cells using the Ambion Purelink RNA mini kit with Purelink homogenizers and Purelink on-column DNAse digestion according to the manufacturer’s specifications (Life Technologies). cDNA was generated using the Invitrogen Superscript III first strand synthesis kit according to the manufacturer’s specifications (Life Technologies). .. Rat Top2b , rat Hprt1 and human RARG gene expression was measured using validated Applied Biosystems TaqMan assays Rn01537914_m1, Rn01527840_ml, and Hs01559234_m1, respectively (Life Technologies). qPCR was performed on the PikoReal 96 Real-Time PCR system (Thermo Scientific) and relative gene expression was calculated by the ΔΔCq method using the instrument software.

    Article Title: Glycinebetaine Biosynthesis in Response to Osmotic Stress Depends on Jasmonate Signaling in Watermelon Suspension Cells
    Article Snippet: Transcripts were amplified by PCR primers that were designed by Primer Premier 5 and listed in Supplementary Table . .. RT-qPCR was carried out using PikoReal 96 Real-Time PCR System (Thermo Fisher ScientificTM , MA, United States). .. To normalize gene expression and quantify gene expression relatively, the 18s rRNA gene (HQ025803.1) of watermelon was used as the reference gene.

    Article Title: Binding of Hemagglutinin and Influenza Virus to a Peptide-Conjugated Lipid Membrane
    Article Snippet: Paragraph title: rRT-PCR ... The reaction was performed using a PikoReal Real-Time PCR system (TCR0096, Thermo Scientific).

    Article Title: S100A12 facilitates osteoclast differentiation from human monocytes
    Article Snippet: Paragraph title: Quantitative real-time polymerase chain reaction (qRT-PCR) ... Quantitative real-time PCR was performed using a QuantiTect SYBR Green PCR kit (Qiagen) with a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol.

    Article Title: Heg1 and Ccm1/2 proteins control endocardial mechanosensitivity during zebrafish valvulogenesis
    Article Snippet: Total RNA was extracted with Trizol (Sigma) and Phase Lock Gel Heavy tubes (1.5 mL, Prime 5) and the corresponding cDNA was synthesized from total RNA with the RevertAid H Minus First Strand cDNA Synthesis kit (ThermoFisher Scientific). .. RT-qPCR experiments were performed as described ( ) using 18 ng cDNA per technical replicate and the KAPA Sybr Fast qPCR kit (Peqlab) on a PikoReal 96 Real-Time PCR System (ThermoFisher Scientific). .. Cycle threshold (Ct) values were determined by PikoReal software 2.2 (ThermoFisher Scientific). eif1b was used as a housekeeping gene.

    Article Title: Genes of ACYL CARRIER PROTEIN Family Show Different Expression Profiles and Overexpression of ACYL CARRIER PROTEIN 5 Modulates Fatty Acid Composition and Enhances Salt Stress Tolerance in Arabidopsis
    Article Snippet: The cDNA was diluted 12 times, and 2 μl were used as a template in a 10 μl PCR reaction. .. Real-time RT-PCR analysis was performed using SYBR Green Perfect mix (TaKaRa, Cat# RR420A) on a Thermo PIKOREAL 96 Real-Time PCR System, with a program of 40 cycles and the following conditions: 95°C for 5 s, 60°C for 30 s with TUA3 (tubulin alpha-3 , At5g19770) as endogenous control. .. The FA composition in seedling tissues was determined as previously described ( ; ) with slight modification.

    SYBR Green Assay:

    Article Title: Antofine suppresses endotoxin‐induced inflammation and metabolic disorder via AMP‐activated protein kinase. Antofine suppresses endotoxin‐induced inflammation and metabolic disorder via AMP‐activated protein kinase
    Article Snippet: The real‐time PCR reactions were performed in 20 μ L volumes containing 10 μ L of Real‐time PCR DyNAmo Flash SYBR Green qPCR reagent (Thermo Fisher Scientific Inc., NYSE: TMO). .. The PCR reaction was performed using a PikoReal™ 96 Real‐Time PCR System (Thermo Fisher Scientific Inc.), and the conditions were as follows: 45 cycles of 95°C for 5 sec and 60°C for 1 min.

    Article Title: Binding of Hemagglutinin and Influenza Virus to a Peptide-Conjugated Lipid Membrane
    Article Snippet: One-step RT-PCR was performed using QuantiTect SYBR Green PCR Kit (QIAGEN) with forward and reverse primers for the sequences of the matrix protein (M) gene for 244 bp (forward M30F2/08: 5′-ATGAGYCTTYTAACCGAGGTCGAAACG-3′; reverse M264R3/08: 5′-TGGACAAANCGTCTACGCTGCAG-3′; Eisfeld et al., ). .. The reaction was performed using a PikoReal Real-Time PCR system (TCR0096, Thermo Scientific).

    Article Title: S100A12 facilitates osteoclast differentiation from human monocytes
    Article Snippet: Total RNA was isolated using an RNeasy Mini kit (Qiagen, Valencia, CA), and 1 μg of the total RNA was reverse-transcribed with a QuantiTect reverse transcription kit (Qiagen). .. Quantitative real-time PCR was performed using a QuantiTect SYBR Green PCR kit (Qiagen) with a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol. .. All primer pairs were purchased from Qiagen.

    Article Title: Dendritic Homeostasis Disruption in a Novel Frontotemporal Dementia Mouse Model Expressing Cytoplasmic Fused in Sarcoma
    Article Snippet: 2.8 The cDNA was synthesized from 1 μg total RNA using the SuperscriptIII First-Strand Synthesis System (Life Technologies). .. Expression levels of glutamate receptor subunit were quantified using the DyNAmo ColorFlash SYBR Green qPCR Kit (Thermo Fisher Scientific) on a PikoReal 96 Real-Time PCR System (Thermo Fisher Scientific). .. Each sample was measured in triplicate and normalized to β-actin levels.

    Article Title: Genes of ACYL CARRIER PROTEIN Family Show Different Expression Profiles and Overexpression of ACYL CARRIER PROTEIN 5 Modulates Fatty Acid Composition and Enhances Salt Stress Tolerance in Arabidopsis
    Article Snippet: The cDNA was diluted 12 times, and 2 μl were used as a template in a 10 μl PCR reaction. .. Real-time RT-PCR analysis was performed using SYBR Green Perfect mix (TaKaRa, Cat# RR420A) on a Thermo PIKOREAL 96 Real-Time PCR System, with a program of 40 cycles and the following conditions: 95°C for 5 s, 60°C for 30 s with TUA3 (tubulin alpha-3 , At5g19770) as endogenous control. .. The FA composition in seedling tissues was determined as previously described ( ; ) with slight modification.

    Incubation:

    Article Title: Binding of Hemagglutinin and Influenza Virus to a Peptide-Conjugated Lipid Membrane
    Article Snippet: After washing with PBS, plates were incubated with AVL viral lysis buffer (QIAamp Viral RNA Mini Kit, QIAGEN) for 10 min. .. The reaction was performed using a PikoReal Real-Time PCR system (TCR0096, Thermo Scientific).

    Article Title: New functionally-enhanced soy proteins as food ingredients with anti-viral activity
    Article Snippet: The RNA was converted to full-length cDNA in the following reaction: 2.5 μl of DMPC (di-methyl pyrocarbonate) water, 5 μl of 5× First Strand buffer (Invitrogen), 0.5 μl of 10 mMdNTP mix (Amersham Biosciences), 2 μl of 50 mM UNi12 primer, 32 U of RNAguard (Amersham Biosciences), 200 U of MMLV reverse transcriptase (Invitrogen) and 5 μl RNA solution in total volume of 25 μl. .. Reactions were incubated at 42 °C for 60 min followed by inactivation of the enzyme at 95 °C for 5 min. PCR amplification with HA gene specific primers (Forward primer: 5′-AGCAAAAGCAGGGGA-3′, Reverse primer: 5′-AGTAGAAACAAGGGTGTT-3′) was performed on the PikoReal 96 Real Time PCR System (Thermo Scientific, USA) to amplify the product containing the fragment HA gene. .. The PCR mix contained: SybrGreen mix 10 μl (Thermo Scientific), 240 nM each of forward primer and reverse primer, ROX 0.04 μl and 5 μl cDNA for a 20 μl reaction volume.

    Luciferase:

    Article Title: A Coding Variant in RARG Confers Susceptibility to Anthracycline-Induced Cardiotoxicity in Childhood Cancer
    Article Snippet: Luminescence assays were developed using the Dual-Glo Luciferase Assay System according to the manufacturer’s specifications (Promega) and read on a POLARstar Omega plate reader (BMG Labtech). .. Rat Top2b , rat Hprt1 and human RARG gene expression was measured using validated Applied Biosystems TaqMan assays Rn01537914_m1, Rn01527840_ml, and Hs01559234_m1, respectively (Life Technologies). qPCR was performed on the PikoReal 96 Real-Time PCR system (Thermo Scientific) and relative gene expression was calculated by the ΔΔCq method using the instrument software.

    Expressing:

    Article Title: Systematic pharmacological screens uncover novel pathways involved in cerebral cavernous malformations
    Article Snippet: Measurements were performed on a PikoReal 96 Real‐Time PCR System (Thermo Fisher Scientific). .. Ct values were determined by PikoReal software 2.2 (Thermo Fisher Scientific).

    Article Title: Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway
    Article Snippet: The mRNA expression of all samples was evaluated by RT-qPCR analysis as previously described ( , ). .. PCR was performed with 10 ng cDNA in a 20- µ l reaction system (2 µ l cDNA, 0.8 µ l 10 µ M forward primer, 0.8 µ l 10 µ M reverse primer, 10 µ l PCR mix buffer, and 6.4 µ l sterilized distilled water) using a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

    Article Title: Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway
    Article Snippet: The mRNA expression of all samples was evaluated by RT-qPCR analysis as previously described ( , ). .. PCR was performed with 10 ng cDNA in a 20-µ l reaction system (2 µ l cDNA, 0.8 µ l 10 µ M forward primer, 0.8 µ l 10 µ M reverse primer, 10 µ l PCR mix buffer, and 6.4 µ l sterilized distilled water) using a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

    Article Title: A Coding Variant in RARG Confers Susceptibility to Anthracycline-Induced Cardiotoxicity in Childhood Cancer
    Article Snippet: For real-time RT-PCR (qPCR) experiments RNA was purified from H9c2 cells using the Ambion Purelink RNA mini kit with Purelink homogenizers and Purelink on-column DNAse digestion according to the manufacturer’s specifications (Life Technologies). cDNA was generated using the Invitrogen Superscript III first strand synthesis kit according to the manufacturer’s specifications (Life Technologies). .. Rat Top2b , rat Hprt1 and human RARG gene expression was measured using validated Applied Biosystems TaqMan assays Rn01537914_m1, Rn01527840_ml, and Hs01559234_m1, respectively (Life Technologies). qPCR was performed on the PikoReal 96 Real-Time PCR system (Thermo Scientific) and relative gene expression was calculated by the ΔΔCq method using the instrument software. .. The anti-DDK 4C5 antibody (catalog # TA50011) was purchased from Origene and the anti-GAPDH antibody (catalog # MAB374) was purchased from Millipore.

    Article Title: Glycinebetaine Biosynthesis in Response to Osmotic Stress Depends on Jasmonate Signaling in Watermelon Suspension Cells
    Article Snippet: Paragraph title: Quantification of Gene Expression ... RT-qPCR was carried out using PikoReal 96 Real-Time PCR System (Thermo Fisher ScientificTM , MA, United States).

    Article Title: Effect of Vipera ammodytes ammodytes Snake Venom on the Human Cytokine Network
    Article Snippet: To determine the expression of genes associated with the human cytokine network, total RNA was isolated from cell cultures treated with 1.0 μg/mL VaaV solution and untreated cell cultures (negative control). .. The isolated RNA was reverse transcribed to cDNA with a PikoReal 96 Real-Time PCR System (Thermo Scientific, Waltham, MA, USA) using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions.

    Article Title: Dendritic Homeostasis Disruption in a Novel Frontotemporal Dementia Mouse Model Expressing Cytoplasmic Fused in Sarcoma
    Article Snippet: 2.8 The cDNA was synthesized from 1 μg total RNA using the SuperscriptIII First-Strand Synthesis System (Life Technologies). .. Expression levels of glutamate receptor subunit were quantified using the DyNAmo ColorFlash SYBR Green qPCR Kit (Thermo Fisher Scientific) on a PikoReal 96 Real-Time PCR System (Thermo Fisher Scientific). .. Each sample was measured in triplicate and normalized to β-actin levels.

    Article Title: Heg1 and Ccm1/2 proteins control endocardial mechanosensitivity during zebrafish valvulogenesis
    Article Snippet: Paragraph title: Quantifications of mRNA expression by RT-qPCR ... RT-qPCR experiments were performed as described ( ) using 18 ng cDNA per technical replicate and the KAPA Sybr Fast qPCR kit (Peqlab) on a PikoReal 96 Real-Time PCR System (ThermoFisher Scientific).

    Transfection:

    Article Title: A Coding Variant in RARG Confers Susceptibility to Anthracycline-Induced Cardiotoxicity in Childhood Cancer
    Article Snippet: Transfections were performed with X-tremeGENE 9 (Roche) or Effectene (Qiagen) reagents according to the manufacturer’s specifications. .. Rat Top2b , rat Hprt1 and human RARG gene expression was measured using validated Applied Biosystems TaqMan assays Rn01537914_m1, Rn01527840_ml, and Hs01559234_m1, respectively (Life Technologies). qPCR was performed on the PikoReal 96 Real-Time PCR system (Thermo Scientific) and relative gene expression was calculated by the ΔΔCq method using the instrument software.

    Cell Culture:

    Article Title: A Coding Variant in RARG Confers Susceptibility to Anthracycline-Induced Cardiotoxicity in Childhood Cancer
    Article Snippet: HEK293T and H9c2(2-1) (ATCC CRL-1446) cells were purchased from ATCC (Cedarlane) and routinely cultured in DMEM supplemented with 10% FBS, 100U/ml Penicillin, 100μg/ml Streptomycin, with additional 0.25μg/ml Amphotericin B for H9c2 cells. .. Rat Top2b , rat Hprt1 and human RARG gene expression was measured using validated Applied Biosystems TaqMan assays Rn01537914_m1, Rn01527840_ml, and Hs01559234_m1, respectively (Life Technologies). qPCR was performed on the PikoReal 96 Real-Time PCR system (Thermo Scientific) and relative gene expression was calculated by the ΔΔCq method using the instrument software.

    Article Title: Antofine suppresses endotoxin‐induced inflammation and metabolic disorder via AMP‐activated protein kinase. Antofine suppresses endotoxin‐induced inflammation and metabolic disorder via AMP‐activated protein kinase
    Article Snippet: Total RNA was extracted from the cultured cells using TRIzol reagent (Invitrogen), and then quantified using an Epoch Microplate Spectrophotometer with a Take3 Micro‐Volume Plate (BioTek, Winooski, VT). .. The PCR reaction was performed using a PikoReal™ 96 Real‐Time PCR System (Thermo Fisher Scientific Inc.), and the conditions were as follows: 45 cycles of 95°C for 5 sec and 60°C for 1 min.

    Hemagglutination Assay:

    Article Title: New functionally-enhanced soy proteins as food ingredients with anti-viral activity
    Article Snippet: The RNA was converted to full-length cDNA in the following reaction: 2.5 μl of DMPC (di-methyl pyrocarbonate) water, 5 μl of 5× First Strand buffer (Invitrogen), 0.5 μl of 10 mMdNTP mix (Amersham Biosciences), 2 μl of 50 mM UNi12 primer, 32 U of RNAguard (Amersham Biosciences), 200 U of MMLV reverse transcriptase (Invitrogen) and 5 μl RNA solution in total volume of 25 μl. .. Reactions were incubated at 42 °C for 60 min followed by inactivation of the enzyme at 95 °C for 5 min. PCR amplification with HA gene specific primers (Forward primer: 5′-AGCAAAAGCAGGGGA-3′, Reverse primer: 5′-AGTAGAAACAAGGGTGTT-3′) was performed on the PikoReal 96 Real Time PCR System (Thermo Scientific, USA) to amplify the product containing the fragment HA gene. .. The PCR mix contained: SybrGreen mix 10 μl (Thermo Scientific), 240 nM each of forward primer and reverse primer, ROX 0.04 μl and 5 μl cDNA for a 20 μl reaction volume.

    Generated:

    Article Title: A Coding Variant in RARG Confers Susceptibility to Anthracycline-Induced Cardiotoxicity in Childhood Cancer
    Article Snippet: For real-time RT-PCR (qPCR) experiments RNA was purified from H9c2 cells using the Ambion Purelink RNA mini kit with Purelink homogenizers and Purelink on-column DNAse digestion according to the manufacturer’s specifications (Life Technologies). cDNA was generated using the Invitrogen Superscript III first strand synthesis kit according to the manufacturer’s specifications (Life Technologies). .. Rat Top2b , rat Hprt1 and human RARG gene expression was measured using validated Applied Biosystems TaqMan assays Rn01537914_m1, Rn01527840_ml, and Hs01559234_m1, respectively (Life Technologies). qPCR was performed on the PikoReal 96 Real-Time PCR system (Thermo Scientific) and relative gene expression was calculated by the ΔΔCq method using the instrument software.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Binding of Hemagglutinin and Influenza Virus to a Peptide-Conjugated Lipid Membrane
    Article Snippet: One-step RT-PCR was performed using QuantiTect SYBR Green PCR Kit (QIAGEN) with forward and reverse primers for the sequences of the matrix protein (M) gene for 244 bp (forward M30F2/08: 5′-ATGAGYCTTYTAACCGAGGTCGAAACG-3′; reverse M264R3/08: 5′-TGGACAAANCGTCTACGCTGCAG-3′; Eisfeld et al., ). .. The reaction was performed using a PikoReal Real-Time PCR system (TCR0096, Thermo Scientific).

    Fluorescence:

    Article Title: Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery, et al. Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery
    Article Snippet: The qPCR assays were conducted using the PikoReal 96 Real‐Time PCR System (Thermo Scientific, USA) or Light Cycler 480 (Roche, Switzerland). .. The qPCR assays were conducted using the PikoReal 96 Real‐Time PCR System (Thermo Scientific, USA) or Light Cycler 480 (Roche, Switzerland).

    Article Title: Binding of Hemagglutinin and Influenza Virus to a Peptide-Conjugated Lipid Membrane
    Article Snippet: The reaction was performed using a PikoReal Real-Time PCR system (TCR0096, Thermo Scientific). .. PCR was set up in a 10-μL reaction volume containing 5 μL of 2 × QuantiTect SYBR Green RT-PCR master mix, 0.1 μL of QuantiTect RT Mix, 0.5 μL of RNA template, 1 μL of forward and reverse primers (10 μM each), and 3.4 μL of RNase free water.

    Article Title: Deletion of psbQ’ gene in Cyanidioschyzon merolae reveals the function of extrinsic PsbQ’ in PSII
    Article Snippet: The real-time quantitative PCR assays were carried out on the PikoReal 96 Real-Time PCR System (Thermo Scientific, USA). .. The real-time quantitative PCR assays were carried out on the PikoReal 96 Real-Time PCR System (Thermo Scientific, USA).

    Isolation:

    Article Title: Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery, et al. Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery
    Article Snippet: 2.7 Total DNA from fecal samples was isolated using a Stool Minicolumn Kit (A & A Biotechnology, Poland). .. The qPCR assays were conducted using the PikoReal 96 Real‐Time PCR System (Thermo Scientific, USA) or Light Cycler 480 (Roche, Switzerland).

    Article Title: Effect of Vipera ammodytes ammodytes Snake Venom on the Human Cytokine Network
    Article Snippet: The obtained RNA concentrations were determined using a Nanodrop 2000 spectrophotometer (Thermo Fischer Scientific, Waltham, MA, USA). .. The isolated RNA was reverse transcribed to cDNA with a PikoReal 96 Real-Time PCR System (Thermo Scientific, Waltham, MA, USA) using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions. .. The PCR program used for cDNA synthesis consisted of sample incubation for 2 min at 50 °C and 10 min at 95 °C, followed by 40 cycles at 95 °C for 15 s and 60 °C for 60 s.

    Article Title: S100A12 facilitates osteoclast differentiation from human monocytes
    Article Snippet: Total RNA was isolated using an RNeasy Mini kit (Qiagen, Valencia, CA), and 1 μg of the total RNA was reverse-transcribed with a QuantiTect reverse transcription kit (Qiagen). .. Quantitative real-time PCR was performed using a QuantiTect SYBR Green PCR kit (Qiagen) with a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol.

    Article Title: Genes of ACYL CARRIER PROTEIN Family Show Different Expression Profiles and Overexpression of ACYL CARRIER PROTEIN 5 Modulates Fatty Acid Composition and Enhances Salt Stress Tolerance in Arabidopsis
    Article Snippet: Paragraph title: Total RNA Isolation and Quantitative Real-Time RT-PCR Analyses ... Real-time RT-PCR analysis was performed using SYBR Green Perfect mix (TaKaRa, Cat# RR420A) on a Thermo PIKOREAL 96 Real-Time PCR System, with a program of 40 cycles and the following conditions: 95°C for 5 s, 60°C for 30 s with TUA3 (tubulin alpha-3 , At5g19770) as endogenous control.

    Negative Control:

    Article Title: Effect of Vipera ammodytes ammodytes Snake Venom on the Human Cytokine Network
    Article Snippet: To determine the expression of genes associated with the human cytokine network, total RNA was isolated from cell cultures treated with 1.0 μg/mL VaaV solution and untreated cell cultures (negative control). .. The isolated RNA was reverse transcribed to cDNA with a PikoReal 96 Real-Time PCR System (Thermo Scientific, Waltham, MA, USA) using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions.

    Size-exclusion Chromatography:

    Article Title: Antofine suppresses endotoxin‐induced inflammation and metabolic disorder via AMP‐activated protein kinase. Antofine suppresses endotoxin‐induced inflammation and metabolic disorder via AMP‐activated protein kinase
    Article Snippet: The real‐time PCR reactions were performed in 20 μ L volumes containing 10 μ L of Real‐time PCR DyNAmo Flash SYBR Green qPCR reagent (Thermo Fisher Scientific Inc., NYSE: TMO). .. The PCR reaction was performed using a PikoReal™ 96 Real‐Time PCR System (Thermo Fisher Scientific Inc.), and the conditions were as follows: 45 cycles of 95°C for 5 sec and 60°C for 1 min. .. The data were analyzed using PiKoReal Software 2.0, exported into Excel for analysis using the ΔCt (the number of PCR cycles to reach the threshold of the product detection) method, and normalized to β ‐actin as an internal control.

    Polymerase Chain Reaction:

    Article Title: Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway
    Article Snippet: The purity and concentration of RNA were quantified using a Nanodrop Spectrophotometer ND-1000 (n=3 per group). cDNA synthesis was performed using a PrimeScript RT reagent kit (cat. no. RR047A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's protocol, with an ARKTIK Thermal Cycler (Thermo Fisher Scientific, Inc.). qPCR was performed using SYBR Premix Ex Taq II (cat. no. RR820A; Takara Biotechnology Co., Ltd.). .. PCR was performed with 10 ng cDNA in a 20- µ l reaction system (2 µ l cDNA, 0.8 µ l 10 µ M forward primer, 0.8 µ l 10 µ M reverse primer, 10 µ l PCR mix buffer, and 6.4 µ l sterilized distilled water) using a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. The PCR conditions were as follows: 2 min of 50°C, 30 sec at 94°C and 40 cycles of 5 sec at 94°C and 34 sec at 60°C.

    Article Title: Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway
    Article Snippet: The purity and concentration of RNA were quantified using a Nanodrop Spectrophotometer ND-1000 (n=3 per group). cDNA synthesis was performed using a PrimeScript RT reagent kit (cat. no. RR047A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's protocol, with an ARKTIK Thermal Cycler (Thermo Fisher Scientific, Inc.). qPCR was performed using SYBR Premix Ex Taq II (cat. no. RR820A; Takara Biotechnology Co., Ltd.). .. PCR was performed with 10 ng cDNA in a 20-µ l reaction system (2 µ l cDNA, 0.8 µ l 10 µ M forward primer, 0.8 µ l 10 µ M reverse primer, 10 µ l PCR mix buffer, and 6.4 µ l sterilized distilled water) using a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. The PCR conditions were as follows: 2 min of 50°C, 30 sec at 94°C and 40 cycles of 5 sec at 94°C and 34 sec at 60°C.

    Article Title: Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery, et al. Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery
    Article Snippet: For the detection of L. salivarius IBB3154 using the classical PCR method, strain‐specific Ls12F, Ls12R, Ls11F, and Ls11R primers were used. .. The qPCR assays were conducted using the PikoReal 96 Real‐Time PCR System (Thermo Scientific, USA) or Light Cycler 480 (Roche, Switzerland).

    Article Title: Glycinebetaine Biosynthesis in Response to Osmotic Stress Depends on Jasmonate Signaling in Watermelon Suspension Cells
    Article Snippet: Transcripts were amplified by PCR primers that were designed by Primer Premier 5 and listed in Supplementary Table . .. RT-qPCR was carried out using PikoReal 96 Real-Time PCR System (Thermo Fisher ScientificTM , MA, United States).

    Article Title: Antofine suppresses endotoxin‐induced inflammation and metabolic disorder via AMP‐activated protein kinase. Antofine suppresses endotoxin‐induced inflammation and metabolic disorder via AMP‐activated protein kinase
    Article Snippet: The real‐time PCR reactions were performed in 20 μ L volumes containing 10 μ L of Real‐time PCR DyNAmo Flash SYBR Green qPCR reagent (Thermo Fisher Scientific Inc., NYSE: TMO). .. The PCR reaction was performed using a PikoReal™ 96 Real‐Time PCR System (Thermo Fisher Scientific Inc.), and the conditions were as follows: 45 cycles of 95°C for 5 sec and 60°C for 1 min. .. The data were analyzed using PiKoReal Software 2.0, exported into Excel for analysis using the ΔCt (the number of PCR cycles to reach the threshold of the product detection) method, and normalized to β ‐actin as an internal control.

    Article Title: Binding of Hemagglutinin and Influenza Virus to a Peptide-Conjugated Lipid Membrane
    Article Snippet: One-step RT-PCR was performed using QuantiTect SYBR Green PCR Kit (QIAGEN) with forward and reverse primers for the sequences of the matrix protein (M) gene for 244 bp (forward M30F2/08: 5′-ATGAGYCTTYTAACCGAGGTCGAAACG-3′; reverse M264R3/08: 5′-TGGACAAANCGTCTACGCTGCAG-3′; Eisfeld et al., ). .. The reaction was performed using a PikoReal Real-Time PCR system (TCR0096, Thermo Scientific).

    Article Title: S100A12 facilitates osteoclast differentiation from human monocytes
    Article Snippet: Total RNA was isolated using an RNeasy Mini kit (Qiagen, Valencia, CA), and 1 μg of the total RNA was reverse-transcribed with a QuantiTect reverse transcription kit (Qiagen). .. Quantitative real-time PCR was performed using a QuantiTect SYBR Green PCR kit (Qiagen) with a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol. .. All primer pairs were purchased from Qiagen.

    Article Title: New functionally-enhanced soy proteins as food ingredients with anti-viral activity
    Article Snippet: The RNA was converted to full-length cDNA in the following reaction: 2.5 μl of DMPC (di-methyl pyrocarbonate) water, 5 μl of 5× First Strand buffer (Invitrogen), 0.5 μl of 10 mMdNTP mix (Amersham Biosciences), 2 μl of 50 mM UNi12 primer, 32 U of RNAguard (Amersham Biosciences), 200 U of MMLV reverse transcriptase (Invitrogen) and 5 μl RNA solution in total volume of 25 μl. .. Reactions were incubated at 42 °C for 60 min followed by inactivation of the enzyme at 95 °C for 5 min. PCR amplification with HA gene specific primers (Forward primer: 5′-AGCAAAAGCAGGGGA-3′, Reverse primer: 5′-AGTAGAAACAAGGGTGTT-3′) was performed on the PikoReal 96 Real Time PCR System (Thermo Scientific, USA) to amplify the product containing the fragment HA gene. .. The PCR mix contained: SybrGreen mix 10 μl (Thermo Scientific), 240 nM each of forward primer and reverse primer, ROX 0.04 μl and 5 μl cDNA for a 20 μl reaction volume.

    Article Title: Genes of ACYL CARRIER PROTEIN Family Show Different Expression Profiles and Overexpression of ACYL CARRIER PROTEIN 5 Modulates Fatty Acid Composition and Enhances Salt Stress Tolerance in Arabidopsis
    Article Snippet: The cDNA was diluted 12 times, and 2 μl were used as a template in a 10 μl PCR reaction. .. Real-time RT-PCR analysis was performed using SYBR Green Perfect mix (TaKaRa, Cat# RR420A) on a Thermo PIKOREAL 96 Real-Time PCR System, with a program of 40 cycles and the following conditions: 95°C for 5 s, 60°C for 30 s with TUA3 (tubulin alpha-3 , At5g19770) as endogenous control.

    Construct:

    Article Title: A Coding Variant in RARG Confers Susceptibility to Anthracycline-Induced Cardiotoxicity in Childhood Cancer
    Article Snippet: Paragraph title: Constructs, cells and reagents ... Rat Top2b , rat Hprt1 and human RARG gene expression was measured using validated Applied Biosystems TaqMan assays Rn01537914_m1, Rn01527840_ml, and Hs01559234_m1, respectively (Life Technologies). qPCR was performed on the PikoReal 96 Real-Time PCR system (Thermo Scientific) and relative gene expression was calculated by the ΔΔCq method using the instrument software.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Genes of ACYL CARRIER PROTEIN Family Show Different Expression Profiles and Overexpression of ACYL CARRIER PROTEIN 5 Modulates Fatty Acid Composition and Enhances Salt Stress Tolerance in Arabidopsis
    Article Snippet: One microgram total RNA was used for reverse transcription (TaKaRa, with gDNA Eraser, CatRP047A). .. Real-time RT-PCR analysis was performed using SYBR Green Perfect mix (TaKaRa, Cat# RR420A) on a Thermo PIKOREAL 96 Real-Time PCR System, with a program of 40 cycles and the following conditions: 95°C for 5 s, 60°C for 30 s with TUA3 (tubulin alpha-3 , At5g19770) as endogenous control.

    Purification:

    Article Title: A Coding Variant in RARG Confers Susceptibility to Anthracycline-Induced Cardiotoxicity in Childhood Cancer
    Article Snippet: For real-time RT-PCR (qPCR) experiments RNA was purified from H9c2 cells using the Ambion Purelink RNA mini kit with Purelink homogenizers and Purelink on-column DNAse digestion according to the manufacturer’s specifications (Life Technologies). cDNA was generated using the Invitrogen Superscript III first strand synthesis kit according to the manufacturer’s specifications (Life Technologies). .. Rat Top2b , rat Hprt1 and human RARG gene expression was measured using validated Applied Biosystems TaqMan assays Rn01537914_m1, Rn01527840_ml, and Hs01559234_m1, respectively (Life Technologies). qPCR was performed on the PikoReal 96 Real-Time PCR system (Thermo Scientific) and relative gene expression was calculated by the ΔΔCq method using the instrument software.

    RNA Extraction:

    Article Title: Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway
    Article Snippet: Paragraph title: RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis ... PCR was performed with 10 ng cDNA in a 20- µ l reaction system (2 µ l cDNA, 0.8 µ l 10 µ M forward primer, 0.8 µ l 10 µ M reverse primer, 10 µ l PCR mix buffer, and 6.4 µ l sterilized distilled water) using a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

    Article Title: Heg1 and Ccm1/2 proteins control endocardial mechanosensitivity during zebrafish valvulogenesis
    Article Snippet: RT-qPCR experiments were performed as described ( ) using 18 ng cDNA per technical replicate and the KAPA Sybr Fast qPCR kit (Peqlab) on a PikoReal 96 Real-Time PCR System (ThermoFisher Scientific). .. RT-qPCR experiments were performed as described ( ) using 18 ng cDNA per technical replicate and the KAPA Sybr Fast qPCR kit (Peqlab) on a PikoReal 96 Real-Time PCR System (ThermoFisher Scientific).

    Article Title: Genes of ACYL CARRIER PROTEIN Family Show Different Expression Profiles and Overexpression of ACYL CARRIER PROTEIN 5 Modulates Fatty Acid Composition and Enhances Salt Stress Tolerance in Arabidopsis
    Article Snippet: Plant samples were collected in liquid nitrogen and stored at -80°C for RNA extraction. .. Real-time RT-PCR analysis was performed using SYBR Green Perfect mix (TaKaRa, Cat# RR420A) on a Thermo PIKOREAL 96 Real-Time PCR System, with a program of 40 cycles and the following conditions: 95°C for 5 s, 60°C for 30 s with TUA3 (tubulin alpha-3 , At5g19770) as endogenous control.

    Software:

    Article Title: A Coding Variant in RARG Confers Susceptibility to Anthracycline-Induced Cardiotoxicity in Childhood Cancer
    Article Snippet: For real-time RT-PCR (qPCR) experiments RNA was purified from H9c2 cells using the Ambion Purelink RNA mini kit with Purelink homogenizers and Purelink on-column DNAse digestion according to the manufacturer’s specifications (Life Technologies). cDNA was generated using the Invitrogen Superscript III first strand synthesis kit according to the manufacturer’s specifications (Life Technologies). .. Rat Top2b , rat Hprt1 and human RARG gene expression was measured using validated Applied Biosystems TaqMan assays Rn01537914_m1, Rn01527840_ml, and Hs01559234_m1, respectively (Life Technologies). qPCR was performed on the PikoReal 96 Real-Time PCR system (Thermo Scientific) and relative gene expression was calculated by the ΔΔCq method using the instrument software. .. The anti-DDK 4C5 antibody (catalog # TA50011) was purchased from Origene and the anti-GAPDH antibody (catalog # MAB374) was purchased from Millipore.

    Real-time Polymerase Chain Reaction:

    Article Title: Systematic pharmacological screens uncover novel pathways involved in cerebral cavernous malformations
    Article Snippet: Quantitative real‐time PCRs (RT‐qPCR) were performed as previously described (Renz et al , ) in compliance with the MIQE standard (Bustin et al , ) with six biological replicates, using 6 ng cDNA per reaction. .. Measurements were performed on a PikoReal 96 Real‐Time PCR System (Thermo Fisher Scientific). .. Ct values were determined by PikoReal software 2.2 (Thermo Fisher Scientific).

    Article Title: Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway
    Article Snippet: The purity and concentration of RNA were quantified using a Nanodrop Spectrophotometer ND-1000 (n=3 per group). cDNA synthesis was performed using a PrimeScript RT reagent kit (cat. no. RR047A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's protocol, with an ARKTIK Thermal Cycler (Thermo Fisher Scientific, Inc.). qPCR was performed using SYBR Premix Ex Taq II (cat. no. RR820A; Takara Biotechnology Co., Ltd.). .. PCR was performed with 10 ng cDNA in a 20- µ l reaction system (2 µ l cDNA, 0.8 µ l 10 µ M forward primer, 0.8 µ l 10 µ M reverse primer, 10 µ l PCR mix buffer, and 6.4 µ l sterilized distilled water) using a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. The PCR conditions were as follows: 2 min of 50°C, 30 sec at 94°C and 40 cycles of 5 sec at 94°C and 34 sec at 60°C.

    Article Title: Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway
    Article Snippet: The purity and concentration of RNA were quantified using a Nanodrop Spectrophotometer ND-1000 (n=3 per group). cDNA synthesis was performed using a PrimeScript RT reagent kit (cat. no. RR047A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's protocol, with an ARKTIK Thermal Cycler (Thermo Fisher Scientific, Inc.). qPCR was performed using SYBR Premix Ex Taq II (cat. no. RR820A; Takara Biotechnology Co., Ltd.). .. PCR was performed with 10 ng cDNA in a 20-µ l reaction system (2 µ l cDNA, 0.8 µ l 10 µ M forward primer, 0.8 µ l 10 µ M reverse primer, 10 µ l PCR mix buffer, and 6.4 µ l sterilized distilled water) using a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. The PCR conditions were as follows: 2 min of 50°C, 30 sec at 94°C and 40 cycles of 5 sec at 94°C and 34 sec at 60°C.

    Article Title: A Coding Variant in RARG Confers Susceptibility to Anthracycline-Induced Cardiotoxicity in Childhood Cancer
    Article Snippet: For real-time RT-PCR (qPCR) experiments RNA was purified from H9c2 cells using the Ambion Purelink RNA mini kit with Purelink homogenizers and Purelink on-column DNAse digestion according to the manufacturer’s specifications (Life Technologies). cDNA was generated using the Invitrogen Superscript III first strand synthesis kit according to the manufacturer’s specifications (Life Technologies). .. Rat Top2b , rat Hprt1 and human RARG gene expression was measured using validated Applied Biosystems TaqMan assays Rn01537914_m1, Rn01527840_ml, and Hs01559234_m1, respectively (Life Technologies). qPCR was performed on the PikoReal 96 Real-Time PCR system (Thermo Scientific) and relative gene expression was calculated by the ΔΔCq method using the instrument software. .. The anti-DDK 4C5 antibody (catalog # TA50011) was purchased from Origene and the anti-GAPDH antibody (catalog # MAB374) was purchased from Millipore.

    Article Title: Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery, et al. Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery
    Article Snippet: To detect L. plantarum IBB3036, strain‐specific Lp13F, Lp13R, Lp18F, and Lp18R primers were used. .. The qPCR assays were conducted using the PikoReal 96 Real‐Time PCR System (Thermo Scientific, USA) or Light Cycler 480 (Roche, Switzerland). .. Primers were designed using the Primer Quest programme ( http://eu.idtdna.com/PrimerQuest/Home/Index ).

    Article Title: Glycinebetaine Biosynthesis in Response to Osmotic Stress Depends on Jasmonate Signaling in Watermelon Suspension Cells
    Article Snippet: Transcripts were amplified by PCR primers that were designed by Primer Premier 5 and listed in Supplementary Table . .. RT-qPCR was carried out using PikoReal 96 Real-Time PCR System (Thermo Fisher ScientificTM , MA, United States). .. To normalize gene expression and quantify gene expression relatively, the 18s rRNA gene (HQ025803.1) of watermelon was used as the reference gene.

    Article Title: Antofine suppresses endotoxin‐induced inflammation and metabolic disorder via AMP‐activated protein kinase. Antofine suppresses endotoxin‐induced inflammation and metabolic disorder via AMP‐activated protein kinase
    Article Snippet: The real‐time PCR reactions were performed in 20 μ L volumes containing 10 μ L of Real‐time PCR DyNAmo Flash SYBR Green qPCR reagent (Thermo Fisher Scientific Inc., NYSE: TMO). .. The PCR reaction was performed using a PikoReal™ 96 Real‐Time PCR System (Thermo Fisher Scientific Inc.), and the conditions were as follows: 45 cycles of 95°C for 5 sec and 60°C for 1 min. .. The data were analyzed using PiKoReal Software 2.0, exported into Excel for analysis using the ΔCt (the number of PCR cycles to reach the threshold of the product detection) method, and normalized to β ‐actin as an internal control.

    Article Title: Binding of Hemagglutinin and Influenza Virus to a Peptide-Conjugated Lipid Membrane
    Article Snippet: One-step RT-PCR was performed using QuantiTect SYBR Green PCR Kit (QIAGEN) with forward and reverse primers for the sequences of the matrix protein (M) gene for 244 bp (forward M30F2/08: 5′-ATGAGYCTTYTAACCGAGGTCGAAACG-3′; reverse M264R3/08: 5′-TGGACAAANCGTCTACGCTGCAG-3′; Eisfeld et al., ). .. The reaction was performed using a PikoReal Real-Time PCR system (TCR0096, Thermo Scientific). .. PCR was set up in a 10-μL reaction volume containing 5 μL of 2 × QuantiTect SYBR Green RT-PCR master mix, 0.1 μL of QuantiTect RT Mix, 0.5 μL of RNA template, 1 μL of forward and reverse primers (10 μM each), and 3.4 μL of RNase free water.

    Article Title: Effect of Vipera ammodytes ammodytes Snake Venom on the Human Cytokine Network
    Article Snippet: The obtained RNA concentrations were determined using a Nanodrop 2000 spectrophotometer (Thermo Fischer Scientific, Waltham, MA, USA). .. The isolated RNA was reverse transcribed to cDNA with a PikoReal 96 Real-Time PCR System (Thermo Scientific, Waltham, MA, USA) using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions. .. The PCR program used for cDNA synthesis consisted of sample incubation for 2 min at 50 °C and 10 min at 95 °C, followed by 40 cycles at 95 °C for 15 s and 60 °C for 60 s.

    Article Title: PSTPIP2 Inhibits the Inflammatory Response and Proliferation of Fibroblast-Like Synoviocytes in vitro
    Article Snippet: Then the cDNA was reversely transcribed using TAKARA (Japan). .. The reaction was prepared following manufacturer’s protocol using SYBRGreen qPCR Master Mix (TAKARA, Japanese) in Pikoreal 96 Real-time PCR system (Thermo Scientific, United States) to detect the level of mRNA. .. The primers which we used were as follows: Rat PSTPIP2, 5’-TGCTGCCCACAIAIACAGAGGA-3’ (forward primer), 5’-TATTGGCACTGCGGTGGACAG-3 (reverse primer).

    Article Title: S100A12 facilitates osteoclast differentiation from human monocytes
    Article Snippet: Total RNA was isolated using an RNeasy Mini kit (Qiagen, Valencia, CA), and 1 μg of the total RNA was reverse-transcribed with a QuantiTect reverse transcription kit (Qiagen). .. Quantitative real-time PCR was performed using a QuantiTect SYBR Green PCR kit (Qiagen) with a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol. .. All primer pairs were purchased from Qiagen.

    Article Title: Dendritic Homeostasis Disruption in a Novel Frontotemporal Dementia Mouse Model Expressing Cytoplasmic Fused in Sarcoma
    Article Snippet: 2.8 The cDNA was synthesized from 1 μg total RNA using the SuperscriptIII First-Strand Synthesis System (Life Technologies). .. Expression levels of glutamate receptor subunit were quantified using the DyNAmo ColorFlash SYBR Green qPCR Kit (Thermo Fisher Scientific) on a PikoReal 96 Real-Time PCR System (Thermo Fisher Scientific). .. Each sample was measured in triplicate and normalized to β-actin levels.

    Article Title: Heg1 and Ccm1/2 proteins control endocardial mechanosensitivity during zebrafish valvulogenesis
    Article Snippet: Total RNA was extracted with Trizol (Sigma) and Phase Lock Gel Heavy tubes (1.5 mL, Prime 5) and the corresponding cDNA was synthesized from total RNA with the RevertAid H Minus First Strand cDNA Synthesis kit (ThermoFisher Scientific). .. RT-qPCR experiments were performed as described ( ) using 18 ng cDNA per technical replicate and the KAPA Sybr Fast qPCR kit (Peqlab) on a PikoReal 96 Real-Time PCR System (ThermoFisher Scientific). .. Cycle threshold (Ct) values were determined by PikoReal software 2.2 (ThermoFisher Scientific). eif1b was used as a housekeeping gene.

    Article Title: New functionally-enhanced soy proteins as food ingredients with anti-viral activity
    Article Snippet: The RNA was converted to full-length cDNA in the following reaction: 2.5 μl of DMPC (di-methyl pyrocarbonate) water, 5 μl of 5× First Strand buffer (Invitrogen), 0.5 μl of 10 mMdNTP mix (Amersham Biosciences), 2 μl of 50 mM UNi12 primer, 32 U of RNAguard (Amersham Biosciences), 200 U of MMLV reverse transcriptase (Invitrogen) and 5 μl RNA solution in total volume of 25 μl. .. Reactions were incubated at 42 °C for 60 min followed by inactivation of the enzyme at 95 °C for 5 min. PCR amplification with HA gene specific primers (Forward primer: 5′-AGCAAAAGCAGGGGA-3′, Reverse primer: 5′-AGTAGAAACAAGGGTGTT-3′) was performed on the PikoReal 96 Real Time PCR System (Thermo Scientific, USA) to amplify the product containing the fragment HA gene. .. The PCR mix contained: SybrGreen mix 10 μl (Thermo Scientific), 240 nM each of forward primer and reverse primer, ROX 0.04 μl and 5 μl cDNA for a 20 μl reaction volume.

    Article Title: Deletion of psbQ’ gene in Cyanidioschyzon merolae reveals the function of extrinsic PsbQ’ in PSII
    Article Snippet: PCRs were performed according to manufacturer’s protocols, supplied with the Phire Plant Direct PCR kit containing, Plant Phire Hot Start II DNA Polymerase (Thermo Fisher Scientific Inc., Waltham, USA) or DreamTaq DNA Polymerase, (Thermo Fisher Scientific Inc. USA). .. The real-time quantitative PCR assays were carried out on the PikoReal 96 Real-Time PCR System (Thermo Scientific, USA). .. Primers were designed in the Primer Quest program ( http://eu.idtdna.com/PrimerQuest/Home/Index ).

    Article Title: Genes of ACYL CARRIER PROTEIN Family Show Different Expression Profiles and Overexpression of ACYL CARRIER PROTEIN 5 Modulates Fatty Acid Composition and Enhances Salt Stress Tolerance in Arabidopsis
    Article Snippet: The cDNA was diluted 12 times, and 2 μl were used as a template in a 10 μl PCR reaction. .. Real-time RT-PCR analysis was performed using SYBR Green Perfect mix (TaKaRa, Cat# RR420A) on a Thermo PIKOREAL 96 Real-Time PCR System, with a program of 40 cycles and the following conditions: 95°C for 5 s, 60°C for 30 s with TUA3 (tubulin alpha-3 , At5g19770) as endogenous control. .. The FA composition in seedling tissues was determined as previously described ( ; ) with slight modification.

    Multiplex Assay:

    Article Title: Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery, et al. Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery
    Article Snippet: To detect the DNA of L. plantarum IBB3036 or L. salivarius IBB3154, multiplex PCR assay was employed, using the primers listed in Table . .. The qPCR assays were conducted using the PikoReal 96 Real‐Time PCR System (Thermo Scientific, USA) or Light Cycler 480 (Roche, Switzerland).

    Spectrophotometry:

    Article Title: Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway
    Article Snippet: The purity and concentration of RNA were quantified using a Nanodrop Spectrophotometer ND-1000 (n=3 per group). cDNA synthesis was performed using a PrimeScript RT reagent kit (cat. no. RR047A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's protocol, with an ARKTIK Thermal Cycler (Thermo Fisher Scientific, Inc.). qPCR was performed using SYBR Premix Ex Taq II (cat. no. RR820A; Takara Biotechnology Co., Ltd.). .. PCR was performed with 10 ng cDNA in a 20- µ l reaction system (2 µ l cDNA, 0.8 µ l 10 µ M forward primer, 0.8 µ l 10 µ M reverse primer, 10 µ l PCR mix buffer, and 6.4 µ l sterilized distilled water) using a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

    Article Title: Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway
    Article Snippet: The purity and concentration of RNA were quantified using a Nanodrop Spectrophotometer ND-1000 (n=3 per group). cDNA synthesis was performed using a PrimeScript RT reagent kit (cat. no. RR047A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's protocol, with an ARKTIK Thermal Cycler (Thermo Fisher Scientific, Inc.). qPCR was performed using SYBR Premix Ex Taq II (cat. no. RR820A; Takara Biotechnology Co., Ltd.). .. PCR was performed with 10 ng cDNA in a 20-µ l reaction system (2 µ l cDNA, 0.8 µ l 10 µ M forward primer, 0.8 µ l 10 µ M reverse primer, 10 µ l PCR mix buffer, and 6.4 µ l sterilized distilled water) using a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

    Article Title: Antofine suppresses endotoxin‐induced inflammation and metabolic disorder via AMP‐activated protein kinase. Antofine suppresses endotoxin‐induced inflammation and metabolic disorder via AMP‐activated protein kinase
    Article Snippet: Total RNA was extracted from the cultured cells using TRIzol reagent (Invitrogen), and then quantified using an Epoch Microplate Spectrophotometer with a Take3 Micro‐Volume Plate (BioTek, Winooski, VT). .. The PCR reaction was performed using a PikoReal™ 96 Real‐Time PCR System (Thermo Fisher Scientific Inc.), and the conditions were as follows: 45 cycles of 95°C for 5 sec and 60°C for 1 min.

    Article Title: Effect of Vipera ammodytes ammodytes Snake Venom on the Human Cytokine Network
    Article Snippet: The obtained RNA concentrations were determined using a Nanodrop 2000 spectrophotometer (Thermo Fischer Scientific, Waltham, MA, USA). .. The isolated RNA was reverse transcribed to cDNA with a PikoReal 96 Real-Time PCR System (Thermo Scientific, Waltham, MA, USA) using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions.

    Article Title: PSTPIP2 Inhibits the Inflammatory Response and Proliferation of Fibroblast-Like Synoviocytes in vitro
    Article Snippet: Total RNA was obtained from synovial tissues and FLSs using TRIZOL reagent (Invitrogen, United States) and quantified by the Thermo Scientific NanodROP 2000 Spectrophotometer (Thermo Scientific, United States). .. The reaction was prepared following manufacturer’s protocol using SYBRGreen qPCR Master Mix (TAKARA, Japanese) in Pikoreal 96 Real-time PCR system (Thermo Scientific, United States) to detect the level of mRNA.

    Concentration Assay:

    Article Title: Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway
    Article Snippet: The purity and concentration of RNA were quantified using a Nanodrop Spectrophotometer ND-1000 (n=3 per group). cDNA synthesis was performed using a PrimeScript RT reagent kit (cat. no. RR047A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's protocol, with an ARKTIK Thermal Cycler (Thermo Fisher Scientific, Inc.). qPCR was performed using SYBR Premix Ex Taq II (cat. no. RR820A; Takara Biotechnology Co., Ltd.). .. PCR was performed with 10 ng cDNA in a 20- µ l reaction system (2 µ l cDNA, 0.8 µ l 10 µ M forward primer, 0.8 µ l 10 µ M reverse primer, 10 µ l PCR mix buffer, and 6.4 µ l sterilized distilled water) using a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

    Article Title: Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway
    Article Snippet: The purity and concentration of RNA were quantified using a Nanodrop Spectrophotometer ND-1000 (n=3 per group). cDNA synthesis was performed using a PrimeScript RT reagent kit (cat. no. RR047A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's protocol, with an ARKTIK Thermal Cycler (Thermo Fisher Scientific, Inc.). qPCR was performed using SYBR Premix Ex Taq II (cat. no. RR820A; Takara Biotechnology Co., Ltd.). .. PCR was performed with 10 ng cDNA in a 20-µ l reaction system (2 µ l cDNA, 0.8 µ l 10 µ M forward primer, 0.8 µ l 10 µ M reverse primer, 10 µ l PCR mix buffer, and 6.4 µ l sterilized distilled water) using a PikoReal 96 Real-Time PCR system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

    Article Title: Glycinebetaine Biosynthesis in Response to Osmotic Stress Depends on Jasmonate Signaling in Watermelon Suspension Cells
    Article Snippet: Ibuprofen (Sigma-Aldrich, Shanghai, China), inhibitor of jasmonate biosynthesis , was dissolved in dimethyl sulfoxide (DMSO) to a final concentration of 50 mM as a stock and Fluridone (FLU), an inhibitor of ABA biosynthesis , was dissolved in distilled water to stock solution of 50 mM. .. RT-qPCR was carried out using PikoReal 96 Real-Time PCR System (Thermo Fisher ScientificTM , MA, United States).

    Lysis:

    Article Title: Binding of Hemagglutinin and Influenza Virus to a Peptide-Conjugated Lipid Membrane
    Article Snippet: After washing with PBS, plates were incubated with AVL viral lysis buffer (QIAamp Viral RNA Mini Kit, QIAGEN) for 10 min. .. The reaction was performed using a PikoReal Real-Time PCR system (TCR0096, Thermo Scientific).

    Fluorescence In Situ Hybridization:

    Article Title: Systematic pharmacological screens uncover novel pathways involved in cerebral cavernous malformations
    Article Snippet: Measurements were performed on a PikoReal 96 Real‐Time PCR System (Thermo Fisher Scientific). .. Measurements were performed on a PikoReal 96 Real‐Time PCR System (Thermo Fisher Scientific).

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    Thermo Fisher pikoreal 96 real time pcr system
    Pikoreal 96 Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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