pikoreal 96 real time pcr system  (Thermo Fisher)


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    PikoReal 96 Real Time PCR System
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    tcr0096
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    Thermo Fisher pikoreal 96 real time pcr system

    https://www.bioz.com/result/pikoreal 96 real time pcr system/product/Thermo Fisher
    Average 99 stars, based on 112 article reviews
    Price from $9.99 to $1999.99
    pikoreal 96 real time pcr system - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Direct detection and characterization of foot‐and‐mouth disease virus in East Africa using a field‐ready real‐time PCR platform, et al. Direct detection and characterization of foot‐and‐mouth disease virus in east Africa using a field‐ready real‐time PCR platform
    Article Snippet: .. In East African laboratories, RNA was extracted using the QIAamp® Viral RNA Mini Kit (Qiagen, UK) and rRT‐PCR reactions performed on either a PikoReal™ Real‐Time PCR System (Thermo Fisher Scientific) (Foot‐and‐Mouth Disease Laboratory, Kenya) or Applied Biosystems® 7500 fast thermal cycler (Applied Biosystems® ) (SUA, Tanzania). .. 2.5.2 rRT‐PCR using the T‐COR™ 8 The FMDV lyophilized pan‐serotype‐specific assay was performed in duplicate using FMDV 2.0 reagents with inhibition control (TC‐9092‐064, Tetracore Inc.).

    Article Title: Visualization of Positive and Negative Sense Viral RNA for Probing the Mechanism of Direct-Acting Antivirals against Hepatitis C Virus
    Article Snippet: .. RC21 and RC1 were replaced by Tag-ZK21 primer (5′-ggccgtcatggtggcgaataaCCTGACAACACTAAaATTGGTGC-3′) and Tag-ZK1 primer (5′-ggccgtcatggtggcgaataaAGGATCATAGGTGATGAAGAAAAGT-3′). cDNA synthesis was performed using SuperScript III First-Strand Synthesis System (Invitrogen), following the manufacturers’ instructions. qPCR amplification was conducted using PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA), in a PikoReal 96 Real-Time PCR system (ThermoFisher Scientific). .. The cycle conditions were uracil-DNA glycosylase (UDG) activation at 50 °C for 2 min, dual-lock DNA polymerase at 95 °C for 2 min, followed by 40 cycles of denaturation at 95 °C for 15 sec, annealing at 55 °C for 15 sec, and extension at 72 °C for 1 min. An MR766 infectious clone [ ] was used to generate a standard curve, and was subject to the same strand specific RT-qPCR protocol.

    Article Title: Expression and clinical significance of Nectin-4 in hepatocellular carcinoma
    Article Snippet: .. Messenger RNA (mRNA) expression of Nectin-4 was performed using SYBR green-based RT-PCR on a PikoReal Real-Time PCR System (Thermo Fisher Scientific). .. The cycling conditions were as follows: an initial denaturation step of 95°C for 3 minutes, followed by 40 amplification cycles involving denaturation at 95°C for 10 seconds, annealing at 60°C for 60 seconds, and elongation at 72°C for 60 seconds.

    Article Title: CLEC14A deficiency exacerbates neuronal loss by increasing blood-brain barrier permeability and inflammation
    Article Snippet: .. Quantitative real-time reverse transcription polymerase chain reaction Total RNA (isolated from WT and CLEC14A-KO mouse brain after ischemia-reperfusion injury) was reverse transcribed to cDNA and amplified with the primers on a PikoReal 96 Real-Time PCR System (Thermo Fisher Scientific) using Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher Scientific; catalog K0221) for quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) assays. .. All qRT-PCR assays were performed in triplicate in at least three independent experiments using two different samples.

    Article Title: Cystathionine-γ-lyase promotes the metastasis of breast cancer via the VEGF signaling pathway
    Article Snippet: .. RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA was extracted from the human breast cancer and adjacent non-tumor tissues, human breast cancer cells and mammary epithelial cells using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), and genomic DNA elimination and cDNA synthesis were performed using PrimeScript™ RT Reagent kit (RR047A; Takara). qPCR was performed using SYBR Premix Ex Taq II, and the PikoReal™ Real-Time PCR system (Thermo Fisher Scientific, Inc.) was used to measure mRNA expression. .. The reaction conditions were as follows: Initial template degeneration, 95°C for 30 sec; followed by 40 cycles at 95°C for 5 sec, 55°C for 30 sec and 72°C for 30 sec; and a final step at 60°C for 30 sec.

    Article Title: Osmotic-Adaptation Response of sakA/hogA Gene to Aflatoxin Biosynthesis, Morphology Development and Pathogenicity in Aspergillus flavus
    Article Snippet: .. To analyze qRT-PCR 2xSYBR Green mix kit (Takara Dalian China), a pair of primers ( ) for target genes were used and amplified with ThermoFisher scientific Real-time PCR system (PikoReal 96 Real-Time PCR system/from Vantaa, Finland). ..

    Article Title: Testing the causality between CYP9M10 and pyrethroid resistance using the TALEN and CRISPR/Cas9 technologies
    Article Snippet: .. PCR was performed in a PikoReal real-time PCR machine (Thermo-Fisher Scientific) at 95 °C for 2 min followed by 40 cycles of 98 °C for 5 s and 60 °C for 60 s. A melting curve was then recorded from 60 to 95 °C with 0.2 °C increment intervals. .. Data was analysed with PikoReal Software 2.2 (Thermo-Fisher Scientific).

    Article Title: Hhip regulates tumor-stroma-mediated upregulation of tumor angiogenesis
    Article Snippet: .. Real-time PCR Total RNA was isolated from B16F10 tumor samples using RNeasy Plus Universal kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. cDNA was synthesized, and real-time PCR was performed on a pikoreal 96 machine (ThermoScientific) using Maxima SYBR green qPCR master mix (ThermoScientific). ..

    RNA Extraction:

    Article Title: Cystathionine-γ-lyase promotes the metastasis of breast cancer via the VEGF signaling pathway
    Article Snippet: .. RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA was extracted from the human breast cancer and adjacent non-tumor tissues, human breast cancer cells and mammary epithelial cells using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), and genomic DNA elimination and cDNA synthesis were performed using PrimeScript™ RT Reagent kit (RR047A; Takara). qPCR was performed using SYBR Premix Ex Taq II, and the PikoReal™ Real-Time PCR system (Thermo Fisher Scientific, Inc.) was used to measure mRNA expression. .. The reaction conditions were as follows: Initial template degeneration, 95°C for 30 sec; followed by 40 cycles at 95°C for 5 sec, 55°C for 30 sec and 72°C for 30 sec; and a final step at 60°C for 30 sec.

    Amplification:

    Article Title: Visualization of Positive and Negative Sense Viral RNA for Probing the Mechanism of Direct-Acting Antivirals against Hepatitis C Virus
    Article Snippet: .. RC21 and RC1 were replaced by Tag-ZK21 primer (5′-ggccgtcatggtggcgaataaCCTGACAACACTAAaATTGGTGC-3′) and Tag-ZK1 primer (5′-ggccgtcatggtggcgaataaAGGATCATAGGTGATGAAGAAAAGT-3′). cDNA synthesis was performed using SuperScript III First-Strand Synthesis System (Invitrogen), following the manufacturers’ instructions. qPCR amplification was conducted using PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA), in a PikoReal 96 Real-Time PCR system (ThermoFisher Scientific). .. The cycle conditions were uracil-DNA glycosylase (UDG) activation at 50 °C for 2 min, dual-lock DNA polymerase at 95 °C for 2 min, followed by 40 cycles of denaturation at 95 °C for 15 sec, annealing at 55 °C for 15 sec, and extension at 72 °C for 1 min. An MR766 infectious clone [ ] was used to generate a standard curve, and was subject to the same strand specific RT-qPCR protocol.

    Article Title: CLEC14A deficiency exacerbates neuronal loss by increasing blood-brain barrier permeability and inflammation
    Article Snippet: .. Quantitative real-time reverse transcription polymerase chain reaction Total RNA (isolated from WT and CLEC14A-KO mouse brain after ischemia-reperfusion injury) was reverse transcribed to cDNA and amplified with the primers on a PikoReal 96 Real-Time PCR System (Thermo Fisher Scientific) using Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher Scientific; catalog K0221) for quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) assays. .. All qRT-PCR assays were performed in triplicate in at least three independent experiments using two different samples.

    Article Title: Osmotic-Adaptation Response of sakA/hogA Gene to Aflatoxin Biosynthesis, Morphology Development and Pathogenicity in Aspergillus flavus
    Article Snippet: .. To analyze qRT-PCR 2xSYBR Green mix kit (Takara Dalian China), a pair of primers ( ) for target genes were used and amplified with ThermoFisher scientific Real-time PCR system (PikoReal 96 Real-Time PCR system/from Vantaa, Finland). ..

    Synthesized:

    Article Title: Hhip regulates tumor-stroma-mediated upregulation of tumor angiogenesis
    Article Snippet: .. Real-time PCR Total RNA was isolated from B16F10 tumor samples using RNeasy Plus Universal kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. cDNA was synthesized, and real-time PCR was performed on a pikoreal 96 machine (ThermoScientific) using Maxima SYBR green qPCR master mix (ThermoScientific). ..

    Isolation:

    Article Title: CLEC14A deficiency exacerbates neuronal loss by increasing blood-brain barrier permeability and inflammation
    Article Snippet: .. Quantitative real-time reverse transcription polymerase chain reaction Total RNA (isolated from WT and CLEC14A-KO mouse brain after ischemia-reperfusion injury) was reverse transcribed to cDNA and amplified with the primers on a PikoReal 96 Real-Time PCR System (Thermo Fisher Scientific) using Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher Scientific; catalog K0221) for quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) assays. .. All qRT-PCR assays were performed in triplicate in at least three independent experiments using two different samples.

    Article Title: Hhip regulates tumor-stroma-mediated upregulation of tumor angiogenesis
    Article Snippet: .. Real-time PCR Total RNA was isolated from B16F10 tumor samples using RNeasy Plus Universal kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. cDNA was synthesized, and real-time PCR was performed on a pikoreal 96 machine (ThermoScientific) using Maxima SYBR green qPCR master mix (ThermoScientific). ..

    Quantitative RT-PCR:

    Article Title: Direct detection and characterization of foot‐and‐mouth disease virus in East Africa using a field‐ready real‐time PCR platform, et al. Direct detection and characterization of foot‐and‐mouth disease virus in east Africa using a field‐ready real‐time PCR platform
    Article Snippet: .. In East African laboratories, RNA was extracted using the QIAamp® Viral RNA Mini Kit (Qiagen, UK) and rRT‐PCR reactions performed on either a PikoReal™ Real‐Time PCR System (Thermo Fisher Scientific) (Foot‐and‐Mouth Disease Laboratory, Kenya) or Applied Biosystems® 7500 fast thermal cycler (Applied Biosystems® ) (SUA, Tanzania). .. 2.5.2 rRT‐PCR using the T‐COR™ 8 The FMDV lyophilized pan‐serotype‐specific assay was performed in duplicate using FMDV 2.0 reagents with inhibition control (TC‐9092‐064, Tetracore Inc.).

    Article Title: CLEC14A deficiency exacerbates neuronal loss by increasing blood-brain barrier permeability and inflammation
    Article Snippet: .. Quantitative real-time reverse transcription polymerase chain reaction Total RNA (isolated from WT and CLEC14A-KO mouse brain after ischemia-reperfusion injury) was reverse transcribed to cDNA and amplified with the primers on a PikoReal 96 Real-Time PCR System (Thermo Fisher Scientific) using Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher Scientific; catalog K0221) for quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) assays. .. All qRT-PCR assays were performed in triplicate in at least three independent experiments using two different samples.

    Article Title: Cystathionine-γ-lyase promotes the metastasis of breast cancer via the VEGF signaling pathway
    Article Snippet: .. RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA was extracted from the human breast cancer and adjacent non-tumor tissues, human breast cancer cells and mammary epithelial cells using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), and genomic DNA elimination and cDNA synthesis were performed using PrimeScript™ RT Reagent kit (RR047A; Takara). qPCR was performed using SYBR Premix Ex Taq II, and the PikoReal™ Real-Time PCR system (Thermo Fisher Scientific, Inc.) was used to measure mRNA expression. .. The reaction conditions were as follows: Initial template degeneration, 95°C for 30 sec; followed by 40 cycles at 95°C for 5 sec, 55°C for 30 sec and 72°C for 30 sec; and a final step at 60°C for 30 sec.

    Article Title: Osmotic-Adaptation Response of sakA/hogA Gene to Aflatoxin Biosynthesis, Morphology Development and Pathogenicity in Aspergillus flavus
    Article Snippet: .. To analyze qRT-PCR 2xSYBR Green mix kit (Takara Dalian China), a pair of primers ( ) for target genes were used and amplified with ThermoFisher scientific Real-time PCR system (PikoReal 96 Real-Time PCR system/from Vantaa, Finland). ..

    SYBR Green Assay:

    Article Title: Visualization of Positive and Negative Sense Viral RNA for Probing the Mechanism of Direct-Acting Antivirals against Hepatitis C Virus
    Article Snippet: .. RC21 and RC1 were replaced by Tag-ZK21 primer (5′-ggccgtcatggtggcgaataaCCTGACAACACTAAaATTGGTGC-3′) and Tag-ZK1 primer (5′-ggccgtcatggtggcgaataaAGGATCATAGGTGATGAAGAAAAGT-3′). cDNA synthesis was performed using SuperScript III First-Strand Synthesis System (Invitrogen), following the manufacturers’ instructions. qPCR amplification was conducted using PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA), in a PikoReal 96 Real-Time PCR system (ThermoFisher Scientific). .. The cycle conditions were uracil-DNA glycosylase (UDG) activation at 50 °C for 2 min, dual-lock DNA polymerase at 95 °C for 2 min, followed by 40 cycles of denaturation at 95 °C for 15 sec, annealing at 55 °C for 15 sec, and extension at 72 °C for 1 min. An MR766 infectious clone [ ] was used to generate a standard curve, and was subject to the same strand specific RT-qPCR protocol.

    Article Title: Hhip regulates tumor-stroma-mediated upregulation of tumor angiogenesis
    Article Snippet: .. Real-time PCR Total RNA was isolated from B16F10 tumor samples using RNeasy Plus Universal kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. cDNA was synthesized, and real-time PCR was performed on a pikoreal 96 machine (ThermoScientific) using Maxima SYBR green qPCR master mix (ThermoScientific). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Expression and clinical significance of Nectin-4 in hepatocellular carcinoma
    Article Snippet: .. Messenger RNA (mRNA) expression of Nectin-4 was performed using SYBR green-based RT-PCR on a PikoReal Real-Time PCR System (Thermo Fisher Scientific). .. The cycling conditions were as follows: an initial denaturation step of 95°C for 3 minutes, followed by 40 amplification cycles involving denaturation at 95°C for 10 seconds, annealing at 60°C for 60 seconds, and elongation at 72°C for 60 seconds.

    Article Title: CLEC14A deficiency exacerbates neuronal loss by increasing blood-brain barrier permeability and inflammation
    Article Snippet: .. Quantitative real-time reverse transcription polymerase chain reaction Total RNA (isolated from WT and CLEC14A-KO mouse brain after ischemia-reperfusion injury) was reverse transcribed to cDNA and amplified with the primers on a PikoReal 96 Real-Time PCR System (Thermo Fisher Scientific) using Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher Scientific; catalog K0221) for quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) assays. .. All qRT-PCR assays were performed in triplicate in at least three independent experiments using two different samples.

    Expressing:

    Article Title: Expression and clinical significance of Nectin-4 in hepatocellular carcinoma
    Article Snippet: .. Messenger RNA (mRNA) expression of Nectin-4 was performed using SYBR green-based RT-PCR on a PikoReal Real-Time PCR System (Thermo Fisher Scientific). .. The cycling conditions were as follows: an initial denaturation step of 95°C for 3 minutes, followed by 40 amplification cycles involving denaturation at 95°C for 10 seconds, annealing at 60°C for 60 seconds, and elongation at 72°C for 60 seconds.

    Article Title: Cystathionine-γ-lyase promotes the metastasis of breast cancer via the VEGF signaling pathway
    Article Snippet: .. RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA was extracted from the human breast cancer and adjacent non-tumor tissues, human breast cancer cells and mammary epithelial cells using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), and genomic DNA elimination and cDNA synthesis were performed using PrimeScript™ RT Reagent kit (RR047A; Takara). qPCR was performed using SYBR Premix Ex Taq II, and the PikoReal™ Real-Time PCR system (Thermo Fisher Scientific, Inc.) was used to measure mRNA expression. .. The reaction conditions were as follows: Initial template degeneration, 95°C for 30 sec; followed by 40 cycles at 95°C for 5 sec, 55°C for 30 sec and 72°C for 30 sec; and a final step at 60°C for 30 sec.

    Polymerase Chain Reaction:

    Article Title: Cystathionine-γ-lyase promotes the metastasis of breast cancer via the VEGF signaling pathway
    Article Snippet: .. RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA was extracted from the human breast cancer and adjacent non-tumor tissues, human breast cancer cells and mammary epithelial cells using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), and genomic DNA elimination and cDNA synthesis were performed using PrimeScript™ RT Reagent kit (RR047A; Takara). qPCR was performed using SYBR Premix Ex Taq II, and the PikoReal™ Real-Time PCR system (Thermo Fisher Scientific, Inc.) was used to measure mRNA expression. .. The reaction conditions were as follows: Initial template degeneration, 95°C for 30 sec; followed by 40 cycles at 95°C for 5 sec, 55°C for 30 sec and 72°C for 30 sec; and a final step at 60°C for 30 sec.

    Article Title: Testing the causality between CYP9M10 and pyrethroid resistance using the TALEN and CRISPR/Cas9 technologies
    Article Snippet: .. PCR was performed in a PikoReal real-time PCR machine (Thermo-Fisher Scientific) at 95 °C for 2 min followed by 40 cycles of 98 °C for 5 s and 60 °C for 60 s. A melting curve was then recorded from 60 to 95 °C with 0.2 °C increment intervals. .. Data was analysed with PikoReal Software 2.2 (Thermo-Fisher Scientific).

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  • 99
    Thermo Fisher pikorea real time pcr system
    Pikorea Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pikorea real time pcr system/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pikorea real time pcr system - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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