pierce streptavidin poly horseradish peroxidase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pierce streptavidin poly horseradish peroxidase
    Pierce Streptavidin Poly Horseradish Peroxidase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pierce streptavidin poly horseradish peroxidase/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pierce streptavidin poly horseradish peroxidase - by Bioz Stars, 2020-03
    88/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Trefoil factor(s) and CA19.9: A promising panel for early detection of pancreatic cancer
    Article Snippet: Paragraph title: 2.4. Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) ... For the detection step, instead of using the manufacturer provided streptavidin-HRP, we used Pierce Streptavidin Poly-HRP (Thermo Scientific, USA), (diluted to 0.4 μl/ml in 1% BSA) and incubated for 20 min in the dark at room temperature.

    Article Title: Microchip-based ultrafast serodiagnostic assay for tuberculosis
    Article Snippet: .. Nunc PolySorp 96-well plates, 1-step ultra TMB ELISA, and Pierce streptavidin poly-HRP were obtained from Thermo Scientific. .. Mycobacterial recombinant 38 kDa and Ag85A proteins were obtained from Mybiosource (CA, USA).

    Article Title: A Combination of MUC5AC and CA19-9 Improves the Diagnosis of Pancreatic Cancer: A Multicenter Study
    Article Snippet: Paragraph title: MUC5AC ELISA ... Plates were again washed with 0.1% PBS-T, and then 100 μl of Pierce Streptavidin Poly-HRP (Thermo Scientific), diluted to 0.0002 mg/ml in 1% BSA, was added to each well and incubated for 30 minutes at room temperature in the dark.

    Flow Cytometry:

    Article Title: Alterations in Inter-Alpha Inhibitor Protein Expression after Hypoxic-Ischemic Brain Injury in Neonatal Rats
    Article Snippet: IAIPs were eluted from the column using a buffer containing 750 mM NaCl, and concentrated and buffer exchanged using a tangential flow filtration system (Labscale, Millipore, Chicago, IL, USA). .. Pierce streptavidin-poly-horseradish-peroxidase (Thermo Scientific, Wilmington, DE, USA) was added to each well to detect the bound biotinylated R-21 pAb and the plate was incubated for 1 h at room temperature.

    Sequencing:

    Article Title: Identification and Characterization of DNA Aptamers Specific for Phosphorylation Epitopes of Tau Protein
    Article Snippet: On the next day, the plate was washed three times with TBST and then treated with 500 nM detecting aptamer or randomly scrambled aptamer sequence (RS) in 100 μ L of PVP buffer (20 mg/mL polyvinylpyrrolidone and 100 μ g/mL salmon sperm in TBST). .. The reaction was left on a shaker for 1 h. Afterward, the wells were washed with TBST three times and 100 μ L of 1:10K diluted Pierce Streptavidin Poly-HRP (Thermo Scientific) solution was added to each well and incubated for 1 h at room temperature.

    Article Title: Identification and Characterization of DNA Aptamers Specific for Phosphorylation Epitopes of Tau Protein
    Article Snippet: On the next day, the plate was washed three times with TBST and then treated with 500 nM detecting aptamer or randomly scrambled aptamer sequence (RS) in 100 μ L of PVP buffer (20 mg/mL polyvinylpyrrolidone and 100 μ g/mL salmon sperm in TBST). .. The reaction was left on a shaker for 1 h. Afterward, the wells were washed with TBST three times and 100 μ L of 1:10K diluted Pierce Streptavidin Poly-HRP (Thermo Scientific) solution was added to each well and incubated for 1 h at room temperature.

    Filtration:

    Article Title: Alterations in Inter-Alpha Inhibitor Protein Expression after Hypoxic-Ischemic Brain Injury in Neonatal Rats
    Article Snippet: IAIPs were eluted from the column using a buffer containing 750 mM NaCl, and concentrated and buffer exchanged using a tangential flow filtration system (Labscale, Millipore, Chicago, IL, USA). .. Pierce streptavidin-poly-horseradish-peroxidase (Thermo Scientific, Wilmington, DE, USA) was added to each well to detect the bound biotinylated R-21 pAb and the plate was incubated for 1 h at room temperature.

    Fluorescence:

    Article Title: EphA2 chimeric antigen receptor-modified T cells for the immunotherapy of esophageal squamous cell carcinoma
    Article Snippet: Then the pierce streptavidin poly-Horseradish peroxidase (ThermoFisher, Waltham, MA) was used to detect. .. Then the pierce streptavidin poly-Horseradish peroxidase (ThermoFisher, Waltham, MA) was used to detect.

    Positive Control:

    Article Title: Trefoil factor(s) and CA19.9: A promising panel for early detection of pancreatic cancer
    Article Snippet: For the detection step, instead of using the manufacturer provided streptavidin-HRP, we used Pierce Streptavidin Poly-HRP (Thermo Scientific, USA), (diluted to 0.4 μl/ml in 1% BSA) and incubated for 20 min in the dark at room temperature. .. Appropriately diluted sample lysates from TFF expressing cell lines were used as a positive control (MCF7 breast cancer cells for TFF1, LS174T colon cancer cell line for TFF2 and AsPC-1 PC cell line for TFF3).

    Sandwich ELISA:

    Article Title: Trefoil factor(s) and CA19.9: A promising panel for early detection of pancreatic cancer
    Article Snippet: Paragraph title: 2.4. Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) ... For the detection step, instead of using the manufacturer provided streptavidin-HRP, we used Pierce Streptavidin Poly-HRP (Thermo Scientific, USA), (diluted to 0.4 μl/ml in 1% BSA) and incubated for 20 min in the dark at room temperature.

    Article Title: Identification and Characterization of DNA Aptamers Specific for Phosphorylation Epitopes of Tau Protein
    Article Snippet: Paragraph title: Aptamer–Antibody Sandwich ELISA. ... The reaction was left on a shaker for 1 h. Afterward, the wells were washed with TBST three times and 100 μ L of 1:10K diluted Pierce Streptavidin Poly-HRP (Thermo Scientific) solution was added to each well and incubated for 1 h at room temperature.

    Immunohistochemistry:

    Article Title: EphA2 chimeric antigen receptor-modified T cells for the immunotherapy of esophageal squamous cell carcinoma
    Article Snippet: Paragraph title: Immunohistochemistry ... Then the pierce streptavidin poly-Horseradish peroxidase (ThermoFisher, Waltham, MA) was used to detect.

    Blocking Assay:

    Article Title: Alterations in Inter-Alpha Inhibitor Protein Expression after Hypoxic-Ischemic Brain Injury in Neonatal Rats
    Article Snippet: After blocking with 200 μL of 5% non-fat dried milk in phosphate buffered saline (PBS) plus 0.05% Tween, 50 μL of sample and the serially diluted IAIPs standards were added to the wells. .. Pierce streptavidin-poly-horseradish-peroxidase (Thermo Scientific, Wilmington, DE, USA) was added to each well to detect the bound biotinylated R-21 pAb and the plate was incubated for 1 h at room temperature.

    Article Title: Identification and Characterization of DNA Aptamers Specific for Phosphorylation Epitopes of Tau Protein
    Article Snippet: After removal of the blocking buffer and washing three times with TBST, different concentrations of Tau441 protein were added into each well (100 μ L). .. The reaction was left on a shaker for 1 h. Afterward, the wells were washed with TBST three times and 100 μ L of 1:10K diluted Pierce Streptavidin Poly-HRP (Thermo Scientific) solution was added to each well and incubated for 1 h at room temperature.

    Article Title: Identification and Characterization of DNA Aptamers Specific for Phosphorylation Epitopes of Tau Protein
    Article Snippet: After removal of the blocking buffer and washing three times with TBST, different concentrations of Tau441 protein were added into each well (100 μ L). .. The reaction was left on a shaker for 1 h. Afterward, the wells were washed with TBST three times and 100 μ L of 1:10K diluted Pierce Streptavidin Poly-HRP (Thermo Scientific) solution was added to each well and incubated for 1 h at room temperature.

    Purification:

    Article Title: Alterations in Inter-Alpha Inhibitor Protein Expression after Hypoxic-Ischemic Brain Injury in Neonatal Rats
    Article Snippet: Purified IAIPs diluted in 100 mM NaPO4 buffer at pH 6.5 and 50 ng per well were coated on ninety-six-well high-binding microplates (Microlon 600, Greiner Bio-One, Monroe, NC, USA) for 1 h at room temperature or overnight at 4°C. .. Pierce streptavidin-poly-horseradish-peroxidase (Thermo Scientific, Wilmington, DE, USA) was added to each well to detect the bound biotinylated R-21 pAb and the plate was incubated for 1 h at room temperature.

    Produced:

    Article Title: Trefoil factor(s) and CA19.9: A promising panel for early detection of pancreatic cancer
    Article Snippet: Standard curves were produced from standard provided with the kit. .. For the detection step, instead of using the manufacturer provided streptavidin-HRP, we used Pierce Streptavidin Poly-HRP (Thermo Scientific, USA), (diluted to 0.4 μl/ml in 1% BSA) and incubated for 20 min in the dark at room temperature.

    Concentration Assay:

    Article Title: A Combination of MUC5AC and CA19-9 Improves the Diagnosis of Pancreatic Cancer: A Multicenter Study
    Article Snippet: Briefly, each well of a 96-well microplate was coated with 100 μl anti-MUC5AC mouse monoclonal antibody (1–13M1) (a generous gift from Dr. Jacques Bara, INSERM U-482, Paris, France), diluted to the working concentration of 10 μg/ml in 0.05M carbonate buffer. .. Plates were again washed with 0.1% PBS-T, and then 100 μl of Pierce Streptavidin Poly-HRP (Thermo Scientific), diluted to 0.0002 mg/ml in 1% BSA, was added to each well and incubated for 30 minutes at room temperature in the dark.

    Incubation:

    Article Title: Alterations in Inter-Alpha Inhibitor Protein Expression after Hypoxic-Ischemic Brain Injury in Neonatal Rats
    Article Snippet: .. Pierce streptavidin-poly-horseradish-peroxidase (Thermo Scientific, Wilmington, DE, USA) was added to each well to detect the bound biotinylated R-21 pAb and the plate was incubated for 1 h at room temperature. .. After washing, 100 μL Enhanced K-Blue TMB substrate (Neogen Corp, Lexington, KY, USA) was added to the wells and the reaction was stopped with 100 μL 1 N HCl solution.

    Article Title: Trefoil factor(s) and CA19.9: A promising panel for early detection of pancreatic cancer
    Article Snippet: .. For the detection step, instead of using the manufacturer provided streptavidin-HRP, we used Pierce Streptavidin Poly-HRP (Thermo Scientific, USA), (diluted to 0.4 μl/ml in 1% BSA) and incubated for 20 min in the dark at room temperature. .. ELISA plates were read at 450 nm with an absorbance correction at 540 nm.

    Article Title: EphA2 chimeric antigen receptor-modified T cells for the immunotherapy of esophageal squamous cell carcinoma
    Article Snippet: Then the pierce streptavidin poly-Horseradish peroxidase (ThermoFisher, Waltham, MA) was used to detect. .. The next day, some tissues were further incubated with rabbit anti-mouse IgG-TRITC secondary antibody (Millipore, Billerica, MA, USA) for 2 h at room temperature.

    Article Title: Identification and Characterization of DNA Aptamers Specific for Phosphorylation Epitopes of Tau Protein
    Article Snippet: .. The reaction was left on a shaker for 1 h. Afterward, the wells were washed with TBST three times and 100 μ L of 1:10K diluted Pierce Streptavidin Poly-HRP (Thermo Scientific) solution was added to each well and incubated for 1 h at room temperature. ..

    Article Title: A Combination of MUC5AC and CA19-9 Improves the Diagnosis of Pancreatic Cancer: A Multicenter Study
    Article Snippet: .. Plates were again washed with 0.1% PBS-T, and then 100 μl of Pierce Streptavidin Poly-HRP (Thermo Scientific), diluted to 0.0002 mg/ml in 1% BSA, was added to each well and incubated for 30 minutes at room temperature in the dark. .. Compared with the modified ELISA assay, in the routine ELISA-assay, normal streptavidin-HRP at 1:200 dilutions in PBS-T in 1% BSA was used.

    Article Title: Identification and Characterization of DNA Aptamers Specific for Phosphorylation Epitopes of Tau Protein
    Article Snippet: .. The reaction was left on a shaker for 1 h. Afterward, the wells were washed with TBST three times and 100 μ L of 1:10K diluted Pierce Streptavidin Poly-HRP (Thermo Scientific) solution was added to each well and incubated for 1 h at room temperature. ..

    Competitive ELISA:

    Article Title: Alterations in Inter-Alpha Inhibitor Protein Expression after Hypoxic-Ischemic Brain Injury in Neonatal Rats
    Article Snippet: Paragraph title: 2.2 Competitive ELISA to measure IAIPs concentrations in rat sera ... Pierce streptavidin-poly-horseradish-peroxidase (Thermo Scientific, Wilmington, DE, USA) was added to each well to detect the bound biotinylated R-21 pAb and the plate was incubated for 1 h at room temperature.

    Microscopy:

    Article Title: EphA2 chimeric antigen receptor-modified T cells for the immunotherapy of esophageal squamous cell carcinoma
    Article Snippet: Then the pierce streptavidin poly-Horseradish peroxidase (ThermoFisher, Waltham, MA) was used to detect. .. Then the pierce streptavidin poly-Horseradish peroxidase (ThermoFisher, Waltham, MA) was used to detect.

    Expressing:

    Article Title: Trefoil factor(s) and CA19.9: A promising panel for early detection of pancreatic cancer
    Article Snippet: For the detection step, instead of using the manufacturer provided streptavidin-HRP, we used Pierce Streptavidin Poly-HRP (Thermo Scientific, USA), (diluted to 0.4 μl/ml in 1% BSA) and incubated for 20 min in the dark at room temperature. .. Appropriately diluted sample lysates from TFF expressing cell lines were used as a positive control (MCF7 breast cancer cells for TFF1, LS174T colon cancer cell line for TFF2 and AsPC-1 PC cell line for TFF3).

    Article Title: A Combination of MUC5AC and CA19-9 Improves the Diagnosis of Pancreatic Cancer: A Multicenter Study
    Article Snippet: The antibody-coated plates were blocked with 250 μl of 3% filtered bovine serum albumin (BSA) for 2.5–3 h at 37 °C, followed by three washes with 0.1% PBS using an automated washer and 100 μl of sample or standards (one-time prepared lysate of A549 cells expressing high levels of MUC5AC), appropriately diluted in 1% BSA, were added to each well. .. Plates were again washed with 0.1% PBS-T, and then 100 μl of Pierce Streptavidin Poly-HRP (Thermo Scientific), diluted to 0.0002 mg/ml in 1% BSA, was added to each well and incubated for 30 minutes at room temperature in the dark.

    Modification:

    Article Title: A Combination of MUC5AC and CA19-9 Improves the Diagnosis of Pancreatic Cancer: A Multicenter Study
    Article Snippet: Plates were again washed with 0.1% PBS-T, and then 100 μl of Pierce Streptavidin Poly-HRP (Thermo Scientific), diluted to 0.0002 mg/ml in 1% BSA, was added to each well and incubated for 30 minutes at room temperature in the dark. .. Compared with the modified ELISA assay, in the routine ELISA-assay, normal streptavidin-HRP at 1:200 dilutions in PBS-T in 1% BSA was used.

    Staining:

    Article Title: EphA2 chimeric antigen receptor-modified T cells for the immunotherapy of esophageal squamous cell carcinoma
    Article Snippet: Then the pierce streptavidin poly-Horseradish peroxidase (ThermoFisher, Waltham, MA) was used to detect. .. Then the pierce streptavidin poly-Horseradish peroxidase (ThermoFisher, Waltham, MA) was used to detect.

    Recombinant:

    Article Title: Microchip-based ultrafast serodiagnostic assay for tuberculosis
    Article Snippet: Nunc PolySorp 96-well plates, 1-step ultra TMB ELISA, and Pierce streptavidin poly-HRP were obtained from Thermo Scientific. .. Mycobacterial recombinant 38 kDa and Ag85A proteins were obtained from Mybiosource (CA, USA).

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  • 90
    Thermo Fisher streptavidin poly hrp
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    Thermo Fisher pierce streptavidin poly horseradish peroxidase
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    https://www.bioz.com/result/pierce streptavidin poly horseradish peroxidase/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pierce streptavidin poly horseradish peroxidase - by Bioz Stars, 2020-03
    88/100 stars
      Buy from Supplier

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