pierce protein concentrators pes 10k mwco  (Thermo Fisher)


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    Name:
    Pierce Protein Concentrators PES
    Description:
    Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone PES membrane for concentration desalting and buffer exchange of biological samples This product has a molecular weight cutoff of 30K and can be used for processing sample volumes up to 0 5 mL Features of Pierce Protein Concentrators PES • Rapid protein concentration sample concentration of 10 to 30 fold can be achieved in 10 minutes or less • Excellent protein recovery unique design minimizes membrane fouling and provides high speed concentrations even with particle laden solutions • Instrument compatible use standard microcentrifuge with fixed angle rotors collect and recover sample without invert spinning • Versatile easy way to perform sample concentration diafiltration or buffer exchange of biological samples For easy identification the MWCO value is etched on the side of each device To assist with sample addition and recovery a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume The vertical design of the membrane allows high flux rates low non specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL Typical protein recovery is 90 when the mass of the proteins in the sample is at least two fold greater than the MWCO of the device used Sample concentration of 10 to 30 fold can be achieved within 10 minutes Applications • Protein concentration with tissue culture media antiserum or monoclonal antibody preparations • Concentration of protein peaks following gel permeation chromatography • Removal of unincorporated label following protein modification reactions • Concentration and desalt buffer exchange after eluting protein from ion exchange hydrophobic interaction HIC metal chelate or affinity chromatography columns • Separating large molecules from small molecules Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two fold greater than the MWCO rating of the PES membrane within the device Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass Protein recovery will vary depending on the specific protein in the sample and its starting concentration To achieve 90 recovery of protein the minimum protein sample concentration should be 0 05 mg mL Pierce Protein Concentrators PES allow accurate control of the dead stop volume 15 µL and final concentration factor providing reliable and consistent sample processing They can be used effectively at a maximum centrifugal force of 15 000 x g at room temperature Centrifugal force temperature and sample volume concentration and viscosity affect filtration rate Optimize centrifugal time for each application Related Products Pierce Protein Concentrators PES 10K MWCO 0 5 mL Pierce Protein Concentrators PES 3K MWCO 0 5 mL Pierce Protein Concentrators PES 100K MWCO 0 5 mL
    Catalog Number:
    88502
    Price:
    None
    Applications:
    Dialysis, Desalting, and Concentration|Protein Biology|Protein Purification & Isolation
    Category:
    Chromatography Columns Resins Spin Filters
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    Structured Review

    Thermo Fisher pierce protein concentrators pes 10k mwco
    Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone PES membrane for concentration desalting and buffer exchange of biological samples This product has a molecular weight cutoff of 30K and can be used for processing sample volumes up to 0 5 mL Features of Pierce Protein Concentrators PES • Rapid protein concentration sample concentration of 10 to 30 fold can be achieved in 10 minutes or less • Excellent protein recovery unique design minimizes membrane fouling and provides high speed concentrations even with particle laden solutions • Instrument compatible use standard microcentrifuge with fixed angle rotors collect and recover sample without invert spinning • Versatile easy way to perform sample concentration diafiltration or buffer exchange of biological samples For easy identification the MWCO value is etched on the side of each device To assist with sample addition and recovery a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume The vertical design of the membrane allows high flux rates low non specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL Typical protein recovery is 90 when the mass of the proteins in the sample is at least two fold greater than the MWCO of the device used Sample concentration of 10 to 30 fold can be achieved within 10 minutes Applications • Protein concentration with tissue culture media antiserum or monoclonal antibody preparations • Concentration of protein peaks following gel permeation chromatography • Removal of unincorporated label following protein modification reactions • Concentration and desalt buffer exchange after eluting protein from ion exchange hydrophobic interaction HIC metal chelate or affinity chromatography columns • Separating large molecules from small molecules Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two fold greater than the MWCO rating of the PES membrane within the device Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass Protein recovery will vary depending on the specific protein in the sample and its starting concentration To achieve 90 recovery of protein the minimum protein sample concentration should be 0 05 mg mL Pierce Protein Concentrators PES allow accurate control of the dead stop volume 15 µL and final concentration factor providing reliable and consistent sample processing They can be used effectively at a maximum centrifugal force of 15 000 x g at room temperature Centrifugal force temperature and sample volume concentration and viscosity affect filtration rate Optimize centrifugal time for each application Related Products Pierce Protein Concentrators PES 10K MWCO 0 5 mL Pierce Protein Concentrators PES 3K MWCO 0 5 mL Pierce Protein Concentrators PES 100K MWCO 0 5 mL
    https://www.bioz.com/result/pierce protein concentrators pes 10k mwco/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pierce protein concentrators pes 10k mwco - by Bioz Stars, 2020-04
    99/100 stars

    Related Products / Commonly Used Together

    sema3c-containing
    control u87t-sema3c cells

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    Related Articles

    Protease Inhibitor:

    Article Title: The Anti-Tumorigenic Activity of Sema3C in the Chick Embryo Chorioallantoic Membrane Model
    Article Snippet: Cells were scratched off the plates in ice-cold phosphate buffered saline (PBS), suspended in RIPA lysis buffer (50 mM TrisHCl (pH 7.5), 150 mM NaCl, 1% Igepal CA-630, 0.5% sodium deoxycholate, 0.1% SDS), supplemented with Halt Protease Inhibitor Cocktail (ThermoFisher Scientific, Rockford, IL, USA, cat. No. 87785), and centrifuged for 40 min at 12.000 g, 4 °C. .. The media collected from Sema3C-containing and control U87t-Sema3C cells were concentrated to 20-fold with Pierce protein concentrators PES 10K MWCO (Thermo-Fisher Scientific, cat. No. 88513), according to the manufacturer’s protocol.

    Purification:

    Article Title: Correcting Errors in the BRCA1 Warning System
    Article Snippet: Coomassie-stained denaturing gels revealed the purified p53R306 monomer ( ) and active tetramers were present on Western blots and in EM images ( ). .. The tetramer population was concentrated by using Pierce concentrators (PES, 100 kDa MWCO; Thermo Scientific).

    Concentration Assay:

    Article Title: The Anti-Tumorigenic Activity of Sema3C in the Chick Embryo Chorioallantoic Membrane Model
    Article Snippet: The concentration of whole cell extract (WCE) proteins (supernatant) was measured by using Bradford reagent (SERVA, Heidelberg, Germany). .. The media collected from Sema3C-containing and control U87t-Sema3C cells were concentrated to 20-fold with Pierce protein concentrators PES 10K MWCO (Thermo-Fisher Scientific, cat. No. 88513), according to the manufacturer’s protocol.

    Incubation:

    Article Title: The Anti-Tumorigenic Activity of Sema3C in the Chick Embryo Chorioallantoic Membrane Model
    Article Snippet: Before analysis, U87t-Sema3C cells were seeded onto 22 cm2 Petri dishes and incubated with 1 µg/mL of tetracycline or without it (control) for 48 hours. .. The media collected from Sema3C-containing and control U87t-Sema3C cells were concentrated to 20-fold with Pierce protein concentrators PES 10K MWCO (Thermo-Fisher Scientific, cat. No. 88513), according to the manufacturer’s protocol.

    Western Blot:

    Article Title: The Anti-Tumorigenic Activity of Sema3C in the Chick Embryo Chorioallantoic Membrane Model
    Article Snippet: Paragraph title: 4.3. Western Blot Analysis ... The media collected from Sema3C-containing and control U87t-Sema3C cells were concentrated to 20-fold with Pierce protein concentrators PES 10K MWCO (Thermo-Fisher Scientific, cat. No. 88513), according to the manufacturer’s protocol.

    Article Title: Correcting Errors in the BRCA1 Warning System
    Article Snippet: Coomassie-stained denaturing gels revealed the purified p53R306 monomer ( ) and active tetramers were present on Western blots and in EM images ( ). .. The tetramer population was concentrated by using Pierce concentrators (PES, 100 kDa MWCO; Thermo Scientific).

    Lysis:

    Article Title: The Anti-Tumorigenic Activity of Sema3C in the Chick Embryo Chorioallantoic Membrane Model
    Article Snippet: Cells were scratched off the plates in ice-cold phosphate buffered saline (PBS), suspended in RIPA lysis buffer (50 mM TrisHCl (pH 7.5), 150 mM NaCl, 1% Igepal CA-630, 0.5% sodium deoxycholate, 0.1% SDS), supplemented with Halt Protease Inhibitor Cocktail (ThermoFisher Scientific, Rockford, IL, USA, cat. No. 87785), and centrifuged for 40 min at 12.000 g, 4 °C. .. The media collected from Sema3C-containing and control U87t-Sema3C cells were concentrated to 20-fold with Pierce protein concentrators PES 10K MWCO (Thermo-Fisher Scientific, cat. No. 88513), according to the manufacturer’s protocol.

    Over Expression:

    Article Title: The Anti-Tumorigenic Activity of Sema3C in the Chick Embryo Chorioallantoic Membrane Model
    Article Snippet: Western Blot Analysis The overexpression of Sema3C protein in U87t-Sema3C cells and secretion to the cell medium was analyzed by Western blot. .. The media collected from Sema3C-containing and control U87t-Sema3C cells were concentrated to 20-fold with Pierce protein concentrators PES 10K MWCO (Thermo-Fisher Scientific, cat. No. 88513), according to the manufacturer’s protocol.

    SDS Page:

    Article Title: The Anti-Tumorigenic Activity of Sema3C in the Chick Embryo Chorioallantoic Membrane Model
    Article Snippet: The media collected from Sema3C-containing and control U87t-Sema3C cells were concentrated to 20-fold with Pierce protein concentrators PES 10K MWCO (Thermo-Fisher Scientific, cat. No. 88513), according to the manufacturer’s protocol. .. Denatured protein samples were loaded onto 7.5% SDS-PAGE and transferred to a nitrocellulose membrane.

    Software:

    Article Title: Correcting Errors in the BRCA1 Warning System
    Article Snippet: The tetramer population was concentrated by using Pierce concentrators (PES, 100 kDa MWCO; Thermo Scientific). .. From the EM images of the tetramers, we selected protein complexes using the RELION software package.

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  • 99
    Thermo Fisher pierce protein concentrators pes 10k mwco
    Pierce Protein Concentrators Pes 10k Mwco, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pierce protein concentrators pes 10k mwco/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pierce protein concentrators pes 10k mwco - by Bioz Stars, 2020-04
    99/100 stars
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