Structured Review

Fisher Scientific pierce protein concentrator
Pierce Protein Concentrator, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pierce protein concentrator/product/Fisher Scientific
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pierce protein concentrator - by Bioz Stars, 2020-04
95/100 stars

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Amplification:

Article Title: C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome
Article Snippet: RNA was reverse transcribed (Fermentas, Distributor: Fisher Scientific Austria GmbH, #K1652) followed by PCR-based amplification of C1R and Sanger sequencing. .. Harvested cell culture supernatants were concentrated 10-fold with Pierce Protein Concentrator (Fisher Scientific Austria GmbH, #88517).

Polymerase Chain Reaction:

Article Title: C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome
Article Snippet: RNA was reverse transcribed (Fermentas, Distributor: Fisher Scientific Austria GmbH, #K1652) followed by PCR-based amplification of C1R and Sanger sequencing. .. Harvested cell culture supernatants were concentrated 10-fold with Pierce Protein Concentrator (Fisher Scientific Austria GmbH, #88517).

Protease Inhibitor:

Article Title: C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome
Article Snippet: Cells were harvested separately and lysed for 10 min on ice in RIPA buffer (50 mM Tris-HCl pH 7.4, 0.154 M NaCl, 1 mM EDTA, 0.5% Triton X-100, 0.05% SDS) containing protease inhibitor (Fisher Scientific Austria GmbH, #87786). .. Harvested cell culture supernatants were concentrated 10-fold with Pierce Protein Concentrator (Fisher Scientific Austria GmbH, #88517).

Bradford Assay:

Article Title: C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome
Article Snippet: Protein concentration of cell lysates was determined by Bradford assay (Biorad Laboratories GmbH, Vienna, Austria, #500-0006). .. Harvested cell culture supernatants were concentrated 10-fold with Pierce Protein Concentrator (Fisher Scientific Austria GmbH, #88517).

Cell Culture:

Article Title: C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome
Article Snippet: .. Harvested cell culture supernatants were concentrated 10-fold with Pierce Protein Concentrator (Fisher Scientific Austria GmbH, #88517). .. Protein concentration of cell culture supernatants was normalized based on the protein concentration of the respective cell lysates.

Purification:

Article Title: C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome
Article Snippet: Harvested cell culture supernatants were concentrated 10-fold with Pierce Protein Concentrator (Fisher Scientific Austria GmbH, #88517). .. C4 purified from human serum (Sigma Aldrich, #C8195) was added at a final concentration of 2.2 ng/ml to harvested cell culture supernatant.

Enzyme-linked Immunosorbent Assay:

Article Title: C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome
Article Snippet: Paragraph title: Patient-Derived Skin Fibroblasts and C4a ELISA ... Harvested cell culture supernatants were concentrated 10-fold with Pierce Protein Concentrator (Fisher Scientific Austria GmbH, #88517).

Concentration Assay:

Article Title: C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome
Article Snippet: Harvested cell culture supernatants were concentrated 10-fold with Pierce Protein Concentrator (Fisher Scientific Austria GmbH, #88517). .. C4 purified from human serum (Sigma Aldrich, #C8195) was added at a final concentration of 2.2 ng/ml to harvested cell culture supernatant.

Protein Concentration:

Article Title: C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome
Article Snippet: Protein concentration of cell lysates was determined by Bradford assay (Biorad Laboratories GmbH, Vienna, Austria, #500-0006). .. Harvested cell culture supernatants were concentrated 10-fold with Pierce Protein Concentrator (Fisher Scientific Austria GmbH, #88517).

Sequencing:

Article Title: C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome
Article Snippet: RNA was reverse transcribed (Fermentas, Distributor: Fisher Scientific Austria GmbH, #K1652) followed by PCR-based amplification of C1R and Sanger sequencing. .. Harvested cell culture supernatants were concentrated 10-fold with Pierce Protein Concentrator (Fisher Scientific Austria GmbH, #88517).

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    Fisher Scientific pierce protein concentrator
    Pierce Protein Concentrator, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pierce protein concentrator/product/Fisher Scientific
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pierce protein concentrator - by Bioz Stars, 2020-04
    95/100 stars
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