pierce 20k mwco protein concentrators  (Thermo Fisher)


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    Name:
    Pierce 20K MWCO Protein Concentrators
    Description:

    Catalog Number:
    89886a
    Price:
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    Structured Review

    Thermo Fisher pierce 20k mwco protein concentrators

    https://www.bioz.com/result/pierce 20k mwco protein concentrators/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pierce 20k mwco protein concentrators - by Bioz Stars, 2020-04
    99/100 stars

    Related Products / Commonly Used Together

    wnt3a-containing fractions
    coomassie gel

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    Related Articles

    Flow Cytometry:

    Article Title: Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
    Article Snippet: Up to 12 L of conditioned medium was 0.45 μm filtered, adjusted to 1% Triton X-100 and applied to an FPLC column containing 200 ml Blue Sepharose 6 Fast Flow (GE Healthcare; 17094801). .. After analysis on a Coomassie gel, Wnt3a-containing fractions were selected, combined and concentrated to 10 ml using Pierce Concentrators 20K MWCO (Thermo Scientific; 89887A).

    Filtration:

    Article Title: Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
    Article Snippet: Human or mouse Wnt3a protein was purchased from R & D Systems, or produced in Drosophila S2 cells grown in suspension culture (gift from Roel Nusse, Stanford University) and purified using Blue Sepharose affinity and gel filtration chromatography. .. After analysis on a Coomassie gel, Wnt3a-containing fractions were selected, combined and concentrated to 10 ml using Pierce Concentrators 20K MWCO (Thermo Scientific; 89887A).

    Chromatography:

    Article Title: Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
    Article Snippet: Human or mouse Wnt3a protein was purchased from R & D Systems, or produced in Drosophila S2 cells grown in suspension culture (gift from Roel Nusse, Stanford University) and purified using Blue Sepharose affinity and gel filtration chromatography. .. After analysis on a Coomassie gel, Wnt3a-containing fractions were selected, combined and concentrated to 10 ml using Pierce Concentrators 20K MWCO (Thermo Scientific; 89887A).

    Synthesized:

    Article Title: A Cell Internalizing Antibody Targeting Capsid Protein (p24) Inhibits the Replication of HIV-1 in T Cells Lines and PBMCs: A Proof of Concept Study
    Article Snippet: Synthesis of antibody-peptide conjugates Cell penetrating peptides ( ) were synthesized at > 90% purity by BioBasic Canada. .. Briefly, antibodies were concentrated to 2 mg/mL by Pierce Concentrator 20 K MWCO (Thermo Scientific, #89886A) and dialyzed against 1 L PBS (pH 6.0) for 1 h at 22°C.

    Centrifugation:

    Article Title: MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination
    Article Snippet: The beads were pelleted by centrifugation for 1 minute and washed 9 times with 10 ml ice-cold PBS each. .. The eluted proteins were concentrated using the Pierce 20K MWCO concentrators (Cat. No. 89886A, Thermo Scientific).

    Purification:

    Article Title: Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
    Article Snippet: Human or mouse Wnt3a protein was purchased from R & D Systems, or produced in Drosophila S2 cells grown in suspension culture (gift from Roel Nusse, Stanford University) and purified using Blue Sepharose affinity and gel filtration chromatography. .. After analysis on a Coomassie gel, Wnt3a-containing fractions were selected, combined and concentrated to 10 ml using Pierce Concentrators 20K MWCO (Thermo Scientific; 89887A).

    Article Title: Fully Quantified Spectral Imaging Reveals in Vivo Membrane Protein Interactions
    Article Snippet: Soluble monomeric YFP and mTurquoise with an N-terminal 6× His tag were expressed and purified to near-milimolar concentrations as described ( ). .. Fluorescent protein stocks were buffer exchanged into PBS buffer with a 20kDa MWCO concentrator (Pierce #87751) and filtered with a 0.2μm syringe filter.

    Article Title: Direct measurements of VEGF–VEGFR2 binding affinities reveal the coupling between ligand binding and receptor dimerization
    Article Snippet: Soluble monomeric YFP with an N-terminal His6 tag was expressed and purified to near-millimolar concentrations as described ( ). .. Fluorescent protein stocks were buffer-exchanged into PBS buffer wit h a 20-kDa-molecular-mass-cutoff concentrator (Pierce, catalog number 87751) and filtered with a 0.2-μm syringe filter.

    Produced:

    Article Title: Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
    Article Snippet: Human or mouse Wnt3a protein was purchased from R & D Systems, or produced in Drosophila S2 cells grown in suspension culture (gift from Roel Nusse, Stanford University) and purified using Blue Sepharose affinity and gel filtration chromatography. .. After analysis on a Coomassie gel, Wnt3a-containing fractions were selected, combined and concentrated to 10 ml using Pierce Concentrators 20K MWCO (Thermo Scientific; 89887A).

    Concentration Assay:

    Article Title: MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination
    Article Snippet: IPTG was added to a final concentration of 0.2 mM and the culture was incubated with shaking (180 rpm) at 18°C for 3 hours. .. The eluted proteins were concentrated using the Pierce 20K MWCO concentrators (Cat. No. 89886A, Thermo Scientific).

    Article Title: A Cell Internalizing Antibody Targeting Capsid Protein (p24) Inhibits the Replication of HIV-1 in T Cells Lines and PBMCs: A Proof of Concept Study
    Article Snippet: Briefly, antibodies were concentrated to 2 mg/mL by Pierce Concentrator 20 K MWCO (Thermo Scientific, #89886A) and dialyzed against 1 L PBS (pH 6.0) for 1 h at 22°C. .. The antibodies were then oxidized by adding sodium periodate (BioBasic Canada, #SB0875) to final concentration of 200 mM and incubating on a rocking platform for 30 min at 4°C in dark.

    Incubation:

    Article Title: MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination
    Article Snippet: 300 µl of glutathione-Sepharose 4B beads (pre-treated with cold PBS) was added to the lysate and incubated with continuous mixing for 2 hours at 4°C. .. The eluted proteins were concentrated using the Pierce 20K MWCO concentrators (Cat. No. 89886A, Thermo Scientific).

    Activity Assay:

    Article Title: Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
    Article Snippet: After analysis on a Coomassie gel, Wnt3a-containing fractions were selected, combined and concentrated to 10 ml using Pierce Concentrators 20K MWCO (Thermo Scientific; 89887A). .. Fractions were analysed for purity and Wnt activity by Coomassie gel and Wnt activity assay (see below), and selected fractions were combined for use.

    Fast Protein Liquid Chromatography:

    Article Title: Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
    Article Snippet: Up to 12 L of conditioned medium was 0.45 μm filtered, adjusted to 1% Triton X-100 and applied to an FPLC column containing 200 ml Blue Sepharose 6 Fast Flow (GE Healthcare; 17094801). .. After analysis on a Coomassie gel, Wnt3a-containing fractions were selected, combined and concentrated to 10 ml using Pierce Concentrators 20K MWCO (Thermo Scientific; 89887A).

    Sonication:

    Article Title: MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination
    Article Snippet: Bacteria were pelleted by centrifugation at 4°C, resuspended in 20 ml cold DPBS buffer (Gibco 14190-136), and sonicated on ice. .. The eluted proteins were concentrated using the Pierce 20K MWCO concentrators (Cat. No. 89886A, Thermo Scientific).

    Recombinant:

    Article Title: MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination
    Article Snippet: Paragraph title: Recombinant proteins ... The eluted proteins were concentrated using the Pierce 20K MWCO concentrators (Cat. No. 89886A, Thermo Scientific).

    Imaging:

    Article Title: Fully Quantified Spectral Imaging Reveals in Vivo Membrane Protein Interactions
    Article Snippet: Fluorescent protein stocks were buffer exchanged into PBS buffer with a 20kDa MWCO concentrator (Pierce #87751) and filtered with a 0.2μm syringe filter. .. For each imaging session, the stocks were then diluted in buffer to micromolar concentrations to produce 100%, 75%, and 50% fluorescent protein solution standards.

    Article Title: Direct measurements of VEGF–VEGFR2 binding affinities reveal the coupling between ligand binding and receptor dimerization
    Article Snippet: Fluorescent protein stocks were buffer-exchanged into PBS buffer wit h a 20-kDa-molecular-mass-cutoff concentrator (Pierce, catalog number 87751) and filtered with a 0.2-μm syringe filter. .. For each imaging session, the stocks were diluted in buffer to micromolar concentrations to produce 100, 75, and 50% fluorescent protein and AF594 solution standards.

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  • 99
    Thermo Fisher pierce 20k mwco protein concentrators
    Pierce 20k Mwco Protein Concentrators, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pierce 20k mwco protein concentrators/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pierce 20k mwco protein concentrators - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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