phusion taq polymerase  (New England Biolabs)


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    Structured Review

    New England Biolabs phusion taq polymerase
    Phusion Taq Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion taq polymerase/product/New England Biolabs
    Average 94 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    phusion taq polymerase - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: CMT-associated mutations in glycyl- and tyrosyl-tRNA synthetases exhibit similar pattern of toxicity and share common genetic modifiers in Drosophila
    Article Snippet: The PCR product was isolated using Qiaquick PCR purification kit (Qiagen) and cloned into the pCR2.1-TOPO (Invitrogen) vector using manufacturers protocols. .. A wild-type TOPO clone was mutagenized with a protocol adapted from the Quickchange Site Directed Mutagenesis kit (Strategene) using Phusion Taq Polymerase and Dpn I (New England Biolabs).

    Amplification:

    Article Title: CMT-associated mutations in glycyl- and tyrosyl-tRNA synthetases exhibit similar pattern of toxicity and share common genetic modifiers in Drosophila
    Article Snippet: The BDGP Drosophila Gene Collection cDNA clone GH09263 was obtained from the Drosophila Genomics Resource Center (Bloomington) and PCR amplified. .. A wild-type TOPO clone was mutagenized with a protocol adapted from the Quickchange Site Directed Mutagenesis kit (Strategene) using Phusion Taq Polymerase and Dpn I (New England Biolabs).

    Synthesized:

    Article Title: Identification of vacuoles containing extraintestinal differentiated forms of Legionella pneumophila in colonized Caenorhabditis elegans soil nematodes
    Article Snippet: Molecular methods, reagents and sequencing Oligonucleotides, synthesized by Invitrogen (Life technologies, Grand Island, NY), are listed in Table . .. All restriction and modifying enzymes, dNTPs, Taq Polymerase, Phusion Taq polymerase and Q5 High Fidelity Polymerase were purchased from New England Biolabs (Whitby, Ontario, Canada).

    Mutagenesis:

    Article Title: CMT-associated mutations in glycyl- and tyrosyl-tRNA synthetases exhibit similar pattern of toxicity and share common genetic modifiers in Drosophila
    Article Snippet: .. A wild-type TOPO clone was mutagenized with a protocol adapted from the Quickchange Site Directed Mutagenesis kit (Strategene) using Phusion Taq Polymerase and Dpn I (New England Biolabs). .. Sequencing analysis identified clones with desired mutations.

    Isolation:

    Article Title: CMT-associated mutations in glycyl- and tyrosyl-tRNA synthetases exhibit similar pattern of toxicity and share common genetic modifiers in Drosophila
    Article Snippet: The PCR product was isolated using Qiaquick PCR purification kit (Qiagen) and cloned into the pCR2.1-TOPO (Invitrogen) vector using manufacturers protocols. .. A wild-type TOPO clone was mutagenized with a protocol adapted from the Quickchange Site Directed Mutagenesis kit (Strategene) using Phusion Taq Polymerase and Dpn I (New England Biolabs).

    Article Title: Identification of vacuoles containing extraintestinal differentiated forms of Legionella pneumophila in colonized Caenorhabditis elegans soil nematodes
    Article Snippet: All restriction and modifying enzymes, dNTPs, Taq Polymerase, Phusion Taq polymerase and Q5 High Fidelity Polymerase were purchased from New England Biolabs (Whitby, Ontario, Canada). .. Plasmid DNA and DNA gel extraction were isolated using QIAprep Spin Miniprep and Gel Extraction kits, respectively, from Qiagen (Toronto, Ontario, Canada).

    Polymerase Chain Reaction:

    Article Title: CMT-associated mutations in glycyl- and tyrosyl-tRNA synthetases exhibit similar pattern of toxicity and share common genetic modifiers in Drosophila
    Article Snippet: The PCR product was isolated using Qiaquick PCR purification kit (Qiagen) and cloned into the pCR2.1-TOPO (Invitrogen) vector using manufacturers protocols. .. A wild-type TOPO clone was mutagenized with a protocol adapted from the Quickchange Site Directed Mutagenesis kit (Strategene) using Phusion Taq Polymerase and Dpn I (New England Biolabs).

    Construct:

    Article Title: CMT-associated mutations in glycyl- and tyrosyl-tRNA synthetases exhibit similar pattern of toxicity and share common genetic modifiers in Drosophila
    Article Snippet: A wild-type TOPO clone was mutagenized with a protocol adapted from the Quickchange Site Directed Mutagenesis kit (Strategene) using Phusion Taq Polymerase and Dpn I (New England Biolabs). .. The Wt and mutant constructs were excised from the TOPO vector and purified using the Qiaquick Gel Extraction kit (Qiagen).

    Purification:

    Article Title: CMT-associated mutations in glycyl- and tyrosyl-tRNA synthetases exhibit similar pattern of toxicity and share common genetic modifiers in Drosophila
    Article Snippet: The PCR product was isolated using Qiaquick PCR purification kit (Qiagen) and cloned into the pCR2.1-TOPO (Invitrogen) vector using manufacturers protocols. .. A wild-type TOPO clone was mutagenized with a protocol adapted from the Quickchange Site Directed Mutagenesis kit (Strategene) using Phusion Taq Polymerase and Dpn I (New England Biolabs).

    Transgenic Assay:

    Article Title: CMT-associated mutations in glycyl- and tyrosyl-tRNA synthetases exhibit similar pattern of toxicity and share common genetic modifiers in Drosophila
    Article Snippet: Paragraph title: Generation of transgenic flies ... A wild-type TOPO clone was mutagenized with a protocol adapted from the Quickchange Site Directed Mutagenesis kit (Strategene) using Phusion Taq Polymerase and Dpn I (New England Biolabs).

    Sequencing:

    Article Title: CMT-associated mutations in glycyl- and tyrosyl-tRNA synthetases exhibit similar pattern of toxicity and share common genetic modifiers in Drosophila
    Article Snippet: The forward primer (Aats-Gly-BglIIF) was designed to ligate to the beginning of the cytoplasmic portion of the Aats-gly cDNA (after the mitochondrial targeting sequence) and to add a Bgl II restriction site and a Drosophila Kozak consensus sequence upstream of the start site; the reverse primer (Aats-Gly-HA- Xho IR) was designed to add a hemagglutinin (HA) epitope tag and a Xho I restriction site. .. A wild-type TOPO clone was mutagenized with a protocol adapted from the Quickchange Site Directed Mutagenesis kit (Strategene) using Phusion Taq Polymerase and Dpn I (New England Biolabs).

    Article Title: Identification of vacuoles containing extraintestinal differentiated forms of Legionella pneumophila in colonized Caenorhabditis elegans soil nematodes
    Article Snippet: Paragraph title: Molecular methods, reagents and sequencing ... All restriction and modifying enzymes, dNTPs, Taq Polymerase, Phusion Taq polymerase and Q5 High Fidelity Polymerase were purchased from New England Biolabs (Whitby, Ontario, Canada).

    Gel Extraction:

    Article Title: CMT-associated mutations in glycyl- and tyrosyl-tRNA synthetases exhibit similar pattern of toxicity and share common genetic modifiers in Drosophila
    Article Snippet: A wild-type TOPO clone was mutagenized with a protocol adapted from the Quickchange Site Directed Mutagenesis kit (Strategene) using Phusion Taq Polymerase and Dpn I (New England Biolabs). .. The Wt and mutant constructs were excised from the TOPO vector and purified using the Qiaquick Gel Extraction kit (Qiagen).

    Article Title: Identification of vacuoles containing extraintestinal differentiated forms of Legionella pneumophila in colonized Caenorhabditis elegans soil nematodes
    Article Snippet: All restriction and modifying enzymes, dNTPs, Taq Polymerase, Phusion Taq polymerase and Q5 High Fidelity Polymerase were purchased from New England Biolabs (Whitby, Ontario, Canada). .. Plasmid DNA and DNA gel extraction were isolated using QIAprep Spin Miniprep and Gel Extraction kits, respectively, from Qiagen (Toronto, Ontario, Canada).

    Chick Chorioallantoic Membrane Assay:

    Article Title: Multiple Envelope Stress Response Pathways Are Activated in an Escherichia coli Strain with Mutations in Two Members of the DedA Membrane Protein Family
    Article Snippet: Phusion/ Taq polymerase, T4 DNA ligase, and all restriction enzymes were purchased from New England BioLabs. .. Ampicillin (Amp) (100 μg/ml), tetracycline (Tet) (12.5 μg/ml), kanamycin (Kan) (30 μg/ml), or chloramphenicol (Cam) (30 μg/ml) was added to the growth medium as necessary.

    Plasmid Preparation:

    Article Title: CMT-associated mutations in glycyl- and tyrosyl-tRNA synthetases exhibit similar pattern of toxicity and share common genetic modifiers in Drosophila
    Article Snippet: The PCR product was isolated using Qiaquick PCR purification kit (Qiagen) and cloned into the pCR2.1-TOPO (Invitrogen) vector using manufacturers protocols. .. A wild-type TOPO clone was mutagenized with a protocol adapted from the Quickchange Site Directed Mutagenesis kit (Strategene) using Phusion Taq Polymerase and Dpn I (New England Biolabs).

    Article Title: Identification of vacuoles containing extraintestinal differentiated forms of Legionella pneumophila in colonized Caenorhabditis elegans soil nematodes
    Article Snippet: All restriction and modifying enzymes, dNTPs, Taq Polymerase, Phusion Taq polymerase and Q5 High Fidelity Polymerase were purchased from New England Biolabs (Whitby, Ontario, Canada). .. Plasmid DNA and DNA gel extraction were isolated using QIAprep Spin Miniprep and Gel Extraction kits, respectively, from Qiagen (Toronto, Ontario, Canada).

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  • 99
    New England Biolabs phusion high fidelity taq polymerase
    Phusion High Fidelity Taq Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion high fidelity taq polymerase/product/New England Biolabs
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    phusion high fidelity taq polymerase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    New England Biolabs phusion taq polymerase
    Phusion Taq Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion taq polymerase/product/New England Biolabs
    Average 94 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    phusion taq polymerase - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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