phusion pfu dna polymerase  (Thermo Fisher)


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  • 99
    Name:
    Pfu DNA Polymerase
    Description:

    Catalog Number:
    ep0501
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    Structured Review

    Thermo Fisher phusion pfu dna polymerase

    https://www.bioz.com/result/phusion pfu dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    phusion pfu dna polymerase - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia
    Article Snippet: .. Standard PCR conditions were as follows: 95 °C for 10 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 30 s], 72 °C for 10 min; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 45 s], 72 °C for 10 min. Quantitative PCR was performed on a Rotor Gene 6000 (Corbett Life Science) using QuantiTect SYBR green Taq polymerase Master Mix (Qiagen) or Pfu DNA polymerase (ThermoFisher) and SYBR green (Sigma-Aldrich). .. Standard PCR conditions were as follows: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 30 s]; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 45 s].

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia
    Article Snippet: .. Standard PCR conditions were as follows: 95 °C for 10 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 30 s], 72 °C for 10 min; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 45 s], 72 °C for 10 min. Quantitative PCR was performed on a Rotor Gene 6000 (Corbett Life Science) using QuantiTect SYBR green Taq polymerase Master Mix (Qiagen) or Pfu DNA polymerase (ThermoFisher) and SYBR green (Sigma-Aldrich). .. Standard PCR conditions were as follows: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 30 s]; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 45 s].

    Amplification:

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia
    Article Snippet: .. Standard PCR conditions were as follows: 95 °C for 10 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 30 s], 72 °C for 10 min; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 45 s], 72 °C for 10 min. Quantitative PCR was performed on a Rotor Gene 6000 (Corbett Life Science) using QuantiTect SYBR green Taq polymerase Master Mix (Qiagen) or Pfu DNA polymerase (ThermoFisher) and SYBR green (Sigma-Aldrich). .. Standard PCR conditions were as follows: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 30 s]; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 45 s].

    Article Title: Cloning and Expression of CD19, a Human B-Cell Marker in NIH-3T3 Cell Line
    Article Snippet: .. For amplification of CD19 cDNA, PCR was performed in a 25 µl reaction solution contained 2.5 µl 10×PCR buffer, 1.5 µl 10 mM dNTPs, 1 µl of each primer (10 pmol/µl ), 0.5 µl Pfu DNA polymerase (10 U/µl ) (Thermo Fisher Scientific, Inc., MA, USA), 1 mM MgSO4 and 1 µl cDNA. .. Each PCR reaction underwent initial denaturation at 95°C for 5 min followed by 39 cycles of denaturation (95°C for 30 s ), annealing (different temperatures for 30 s ) and extension (72°C for 2.5 min ), and final extension at 72°C for 10 min .

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia
    Article Snippet: .. Standard PCR conditions were as follows: 95 °C for 10 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 30 s], 72 °C for 10 min; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 45 s], 72 °C for 10 min. Quantitative PCR was performed on a Rotor Gene 6000 (Corbett Life Science) using QuantiTect SYBR green Taq polymerase Master Mix (Qiagen) or Pfu DNA polymerase (ThermoFisher) and SYBR green (Sigma-Aldrich). .. Standard PCR conditions were as follows: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 30 s]; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 45 s].

    Mutagenesis:

    Article Title: Glycerate kinase of the hyperthermophilic archaeon Thermoproteus tenax: new insights into the phylogenetic distribution and physiological role of members of the three different glycerate kinase classes
    Article Snippet: .. PCR mutagenesis was performed using Pfu polymerase (Fermentas) and genomic DNA from T. tenax as template. .. The sequence of the cloned gene was confirmed by dideoxy sequencing of both strands.

    SYBR Green Assay:

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia
    Article Snippet: .. Standard PCR conditions were as follows: 95 °C for 10 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 30 s], 72 °C for 10 min; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 45 s], 72 °C for 10 min. Quantitative PCR was performed on a Rotor Gene 6000 (Corbett Life Science) using QuantiTect SYBR green Taq polymerase Master Mix (Qiagen) or Pfu DNA polymerase (ThermoFisher) and SYBR green (Sigma-Aldrich). .. Standard PCR conditions were as follows: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 30 s]; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 45 s].

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia
    Article Snippet: .. Standard PCR conditions were as follows: 95 °C for 10 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 30 s], 72 °C for 10 min; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 45 s], 72 °C for 10 min. Quantitative PCR was performed on a Rotor Gene 6000 (Corbett Life Science) using QuantiTect SYBR green Taq polymerase Master Mix (Qiagen) or Pfu DNA polymerase (ThermoFisher) and SYBR green (Sigma-Aldrich). .. Standard PCR conditions were as follows: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 30 s]; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 45 s].

    Polymerase Chain Reaction:

    Article Title: Glycerate kinase of the hyperthermophilic archaeon Thermoproteus tenax: new insights into the phylogenetic distribution and physiological role of members of the three different glycerate kinase classes
    Article Snippet: .. PCR mutagenesis was performed using Pfu polymerase (Fermentas) and genomic DNA from T. tenax as template. .. The sequence of the cloned gene was confirmed by dideoxy sequencing of both strands.

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia
    Article Snippet: .. Standard PCR conditions were as follows: 95 °C for 10 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 30 s], 72 °C for 10 min; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 45 s], 72 °C for 10 min. Quantitative PCR was performed on a Rotor Gene 6000 (Corbett Life Science) using QuantiTect SYBR green Taq polymerase Master Mix (Qiagen) or Pfu DNA polymerase (ThermoFisher) and SYBR green (Sigma-Aldrich). .. Standard PCR conditions were as follows: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 30 s]; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 45 s].

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia
    Article Snippet: .. End-point PCR was carried out on a VWR Doppio PCR device using Maxima Hot Start Taq Polymerase (ThermoFisher) or Pfu DNA Polymerase (ThermoFisher). .. Standard PCR conditions were as follows: 95 °C for 10 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 30 s], 72 °C for 10 min; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 45 s], 72 °C for 10 min. Quantitative PCR was performed on a Rotor Gene 6000 (Corbett Life Science) using QuantiTect SYBR green Taq polymerase Master Mix (Qiagen) or Pfu DNA polymerase (ThermoFisher) and SYBR green (Sigma-Aldrich).

    Article Title: Cloning and Expression of CD19, a Human B-Cell Marker in NIH-3T3 Cell Line
    Article Snippet: .. For amplification of CD19 cDNA, PCR was performed in a 25 µl reaction solution contained 2.5 µl 10×PCR buffer, 1.5 µl 10 mM dNTPs, 1 µl of each primer (10 pmol/µl ), 0.5 µl Pfu DNA polymerase (10 U/µl ) (Thermo Fisher Scientific, Inc., MA, USA), 1 mM MgSO4 and 1 µl cDNA. .. Each PCR reaction underwent initial denaturation at 95°C for 5 min followed by 39 cycles of denaturation (95°C for 30 s ), annealing (different temperatures for 30 s ) and extension (72°C for 2.5 min ), and final extension at 72°C for 10 min .

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia
    Article Snippet: .. Standard PCR conditions were as follows: 95 °C for 10 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 30 s], 72 °C for 10 min; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 45 s], 72 °C for 10 min. Quantitative PCR was performed on a Rotor Gene 6000 (Corbett Life Science) using QuantiTect SYBR green Taq polymerase Master Mix (Qiagen) or Pfu DNA polymerase (ThermoFisher) and SYBR green (Sigma-Aldrich). .. Standard PCR conditions were as follows: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 30 s]; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 40 cycles of [95 °C for 15 s, 60 °C for 45 s].

    other:

    Article Title: Simple and Rapid Enzymatic Method for the Synthesis of Single-Strand Oligonucleotides Containing Trifluorothymidine
    Article Snippet: Pfu DNA polymerase was purchased from Fermentas life Sciences (USA).

    Marker:

    Article Title: The Human Cytomegalovirus UL133-138 Gene Locus Attenuates the Lytic Viral Cycle in Fibroblasts
    Article Snippet: .. To remove the RspL-neo marker cassette, another set of primer pairs with overlapping 3’ ends were used to make a small stretch of double stranded DNA by Pfu DNA polymerase (Life Technologies, Grand Island, NY) extension with oligonucleotides homologous to both ends of the targeted region. .. Gel purified linear double-stranded DNA was electroporated into recombination competent DY380 carrying the intermediate rHCMV-BAC DNA for second stage recombination.

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  • 99
    Thermo Fisher phusion hot start ii hf pfu polymerase
    Phusion Hot Start Ii Hf Pfu Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion hot start ii hf pfu polymerase/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    phusion hot start ii hf pfu polymerase - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher phusion pfu dna polymerase
    Phusion Pfu Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion pfu dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    phusion pfu dna polymerase - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

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