phusion high fidelity pcr kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher phusion high fidelity pcr kit
    Phusion High Fidelity Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion high fidelity pcr kit/product/Thermo Fisher
    Average 99 stars, based on 71 article reviews
    Price from $9.99 to $1999.99
    phusion high fidelity pcr kit - by Bioz Stars, 2020-03
    99/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: qnrD, a Novel Gene Conferring Transferable Quinolone Resistance in Salmonella enterica Serovar Kentucky and Bovismorbificans Strains of Human Origin ▿
    Article Snippet: .. The cloned fragments were amplified using plasmid DNA as a template for a long PCR (Phusion high-fidelity PCR kit; Finnzymes, Espoo, Finland) with primers designed with the kanamycin resistance gene to amplify the insert and the flanking regions of the SmaI restriction site (Table ) with HF buffer (Phusion high-fidelity PCR kit; Finnzymes, Espoo, Finland) and under normal conditions as indicated by the manufacturer, at an annealing temperature of 50°C, allowing an extension time of 4 min. Sequencing of the initial fragments in both senses was performed with the primers used for the amplification of the cloned fragment. ..

    Negative Control:

    Article Title: Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor
    Article Snippet: The following thermal cycler PCR protocol was used with a Phusion High-Fidelity PCR Kit (Thermo Fisher Scientific): (1) 98 °C for 30 s; (2) 98 °C for 10 s; (3) 63.5 °C for 10 s; (4) 72 °C for 10 s; (5) 72 °C for 2 min; (6) hold at 4 °C; and (7) repeat steps (2)–(4) 30×. .. As a negative control, PCR was also performed on 900 ng of HEK DNA.

    Amplification:

    Article Title: Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor
    Article Snippet: Paragraph title: Genomic bfp PCR amplification ... The following thermal cycler PCR protocol was used with a Phusion High-Fidelity PCR Kit (Thermo Fisher Scientific): (1) 98 °C for 30 s; (2) 98 °C for 10 s; (3) 63.5 °C for 10 s; (4) 72 °C for 10 s; (5) 72 °C for 2 min; (6) hold at 4 °C; and (7) repeat steps (2)–(4) 30×.

    Article Title: qnrD, a Novel Gene Conferring Transferable Quinolone Resistance in Salmonella enterica Serovar Kentucky and Bovismorbificans Strains of Human Origin ▿
    Article Snippet: .. The cloned fragments were amplified using plasmid DNA as a template for a long PCR (Phusion high-fidelity PCR kit; Finnzymes, Espoo, Finland) with primers designed with the kanamycin resistance gene to amplify the insert and the flanking regions of the SmaI restriction site (Table ) with HF buffer (Phusion high-fidelity PCR kit; Finnzymes, Espoo, Finland) and under normal conditions as indicated by the manufacturer, at an annealing temperature of 50°C, allowing an extension time of 4 min. Sequencing of the initial fragments in both senses was performed with the primers used for the amplification of the cloned fragment. ..

    Article Title: Genetic structure of the European hedgehog (Erinaceus europaeus) in Denmark
    Article Snippet: .. The ligated samples (50μL containing approximately 50 ng DNA pooled from 4 separate samples) were purified with AMPure XP beads (Beckman Coulter, USA) and amplified using the Phusion High-Fidelity PCR kit (Thermo Scientific, USA). ..

    Article Title: Broad dengue neutralization in mosquitoes expressing an engineered antibody
    Article Snippet: WT mosquitoes served as controls to ensure that the WT locus was successfully amplified in the genetic background. .. A PCR kit (ThermoFisher Scientific #F553S) with a 57°C annealing temperature was used for all PCRs following standard protocols.

    Sample Prep:

    Article Title: Genetic structure of the European hedgehog (Erinaceus europaeus) in Denmark
    Article Snippet: Paragraph title: Sample preparation ... The ligated samples (50μL containing approximately 50 ng DNA pooled from 4 separate samples) were purified with AMPure XP beads (Beckman Coulter, USA) and amplified using the Phusion High-Fidelity PCR kit (Thermo Scientific, USA).

    Isolation:

    Article Title: qnrD, a Novel Gene Conferring Transferable Quinolone Resistance in Salmonella enterica Serovar Kentucky and Bovismorbificans Strains of Human Origin ▿
    Article Snippet: The cloned fragments were amplified using plasmid DNA as a template for a long PCR (Phusion high-fidelity PCR kit; Finnzymes, Espoo, Finland) with primers designed with the kanamycin resistance gene to amplify the insert and the flanking regions of the SmaI restriction site (Table ) with HF buffer (Phusion high-fidelity PCR kit; Finnzymes, Espoo, Finland) and under normal conditions as indicated by the manufacturer, at an annealing temperature of 50°C, allowing an extension time of 4 min. Sequencing of the initial fragments in both senses was performed with the primers used for the amplification of the cloned fragment. .. Furthermore, primer walking was also performed for the remaining portion of the isolated plasmid, designing primers directed outwards from the cloned fragment that were used to amplify the remaining sequence using the plasmid DNA extracted from Salmonella isolate GSS-HN-2007-057 as a template, and further primers designed from the previous sequences were used to obtain the total sequence of the plasmid.

    Purification:

    Article Title: Genetic structure of the European hedgehog (Erinaceus europaeus) in Denmark
    Article Snippet: .. The ligated samples (50μL containing approximately 50 ng DNA pooled from 4 separate samples) were purified with AMPure XP beads (Beckman Coulter, USA) and amplified using the Phusion High-Fidelity PCR kit (Thermo Scientific, USA). ..

    Polymerase Chain Reaction:

    Article Title: Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor
    Article Snippet: .. The following thermal cycler PCR protocol was used with a Phusion High-Fidelity PCR Kit (Thermo Fisher Scientific): (1) 98 °C for 30 s; (2) 98 °C for 10 s; (3) 63.5 °C for 10 s; (4) 72 °C for 10 s; (5) 72 °C for 2 min; (6) hold at 4 °C; and (7) repeat steps (2)–(4) 30×. .. For PCR in the presence of HEK DNA contamination, bfp was amplified from a mixture of 900 ng of HEK-BFP and 900 ng of HEK DNA.

    Article Title: qnrD, a Novel Gene Conferring Transferable Quinolone Resistance in Salmonella enterica Serovar Kentucky and Bovismorbificans Strains of Human Origin ▿
    Article Snippet: .. The cloned fragments were amplified using plasmid DNA as a template for a long PCR (Phusion high-fidelity PCR kit; Finnzymes, Espoo, Finland) with primers designed with the kanamycin resistance gene to amplify the insert and the flanking regions of the SmaI restriction site (Table ) with HF buffer (Phusion high-fidelity PCR kit; Finnzymes, Espoo, Finland) and under normal conditions as indicated by the manufacturer, at an annealing temperature of 50°C, allowing an extension time of 4 min. Sequencing of the initial fragments in both senses was performed with the primers used for the amplification of the cloned fragment. ..

    Article Title: Genetic structure of the European hedgehog (Erinaceus europaeus) in Denmark
    Article Snippet: .. The ligated samples (50μL containing approximately 50 ng DNA pooled from 4 separate samples) were purified with AMPure XP beads (Beckman Coulter, USA) and amplified using the Phusion High-Fidelity PCR kit (Thermo Scientific, USA). ..

    Article Title: Broad dengue neutralization in mosquitoes expressing an engineered antibody
    Article Snippet: .. A PCR kit (ThermoFisher Scientific #F553S) with a 57°C annealing temperature was used for all PCRs following standard protocols. .. Generation of w Mel Wolbachia line and infection assay Eggs of Ae . aegypti infected with the Wolbachia strain w Mel were obtained from the World Mosquito Program (Prof. Scott O’Neill, Monash University).

    Sequencing:

    Article Title: qnrD, a Novel Gene Conferring Transferable Quinolone Resistance in Salmonella enterica Serovar Kentucky and Bovismorbificans Strains of Human Origin ▿
    Article Snippet: .. The cloned fragments were amplified using plasmid DNA as a template for a long PCR (Phusion high-fidelity PCR kit; Finnzymes, Espoo, Finland) with primers designed with the kanamycin resistance gene to amplify the insert and the flanking regions of the SmaI restriction site (Table ) with HF buffer (Phusion high-fidelity PCR kit; Finnzymes, Espoo, Finland) and under normal conditions as indicated by the manufacturer, at an annealing temperature of 50°C, allowing an extension time of 4 min. Sequencing of the initial fragments in both senses was performed with the primers used for the amplification of the cloned fragment. ..

    Article Title: Genetic structure of the European hedgehog (Erinaceus europaeus) in Denmark
    Article Snippet: The ligated samples (50μL containing approximately 50 ng DNA pooled from 4 separate samples) were purified with AMPure XP beads (Beckman Coulter, USA) and amplified using the Phusion High-Fidelity PCR kit (Thermo Scientific, USA). .. Paired-end (2x151 bp) sequencing was performed on an Illumina HiSeq X platform by Admera Health (USA).

    Plasmid Preparation:

    Article Title: qnrD, a Novel Gene Conferring Transferable Quinolone Resistance in Salmonella enterica Serovar Kentucky and Bovismorbificans Strains of Human Origin ▿
    Article Snippet: .. The cloned fragments were amplified using plasmid DNA as a template for a long PCR (Phusion high-fidelity PCR kit; Finnzymes, Espoo, Finland) with primers designed with the kanamycin resistance gene to amplify the insert and the flanking regions of the SmaI restriction site (Table ) with HF buffer (Phusion high-fidelity PCR kit; Finnzymes, Espoo, Finland) and under normal conditions as indicated by the manufacturer, at an annealing temperature of 50°C, allowing an extension time of 4 min. Sequencing of the initial fragments in both senses was performed with the primers used for the amplification of the cloned fragment. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher phusion high fidelity pcr kit
    Phusion High Fidelity Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion high fidelity pcr kit/product/Thermo Fisher
    Average 99 stars, based on 71 article reviews
    Price from $9.99 to $1999.99
    phusion high fidelity pcr kit - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results