phusion high fidelity enzyme  (New England Biolabs)


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    Name:
    Phusion High Fidelity PCR Kit
    Description:
    Phusion High Fidelity PCR Kit 200 rxns 50 ul vol
    Catalog Number:
    e0553l
    Price:
    248
    Size:
    200 rxns
    Category:
    PCR related Kits
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    Structured Review

    New England Biolabs phusion high fidelity enzyme
    Phusion High Fidelity PCR Kit
    Phusion High Fidelity PCR Kit 200 rxns 50 ul vol
    https://www.bioz.com/result/phusion high fidelity enzyme/product/New England Biolabs
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    phusion high fidelity enzyme - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Selection of an Efficient AAV Vector for Robust CNS Transgene Expression
    Article Snippet: .. After DNA extraction, the Cap9 inserts (containing the 21-mer sequence encoding the 7-mer peptides) were amplified using the following primers: Cap9_Kpn/Age_For: 5′-AGCTACCGACAACAACGTGT-3′ and Cap9_ Kpn/Age_Rev: 5′- AGAAGGGTGAAAGTTGCCGT-3′ (Phusion high-fidelity PCR kit, New England Biolabs). .. The amplicons were then purified (Monarch PCR and DNA cleanup kit, New England Biolabs), digested by KpnI, AgeI, and BanII, and the Cap9 KpnI-AgeI fragments (144 bp) were agarose gel purified (Monarch DNA gel extraction kit, New England Biolabs) before ligation in the pUC57-Cap9-XbaI/AgeI/KpnI plasmid (opened with KpnI and AgeI and dephosphorylated with calf inositol phosphatase, New England Biolabs).

    Clone Assay:

    Article Title: A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress
    Article Snippet: .. The CgHSP70 ORF, lacking its stop codon, was amplified using a Phusion® High-Fidelity PCR kit (New England Biolabs, Ipswich, MA, USA) with the primer pair CgHSP-Dra-F/-Not-R , and the resulting amplicon cloned into pMD19-T for validation by sequencing. .. The same fragment was also inserted into the Dra I and Not I cloning sites of the pENTR™ 1A dual selection vector (Invitrogen, Carlsbad, CA, USA) using T4 DNA ligase (TaKaRa, Tokyo, Japan), and from there introduced into pEarleyGate 103 using LR Clonase™ II enzyme mix (Invitrogen, Carlsbad, CA, USA) [ ].

    Article Title: Creating single-copy genetic circuits
    Article Snippet: .. Restriction enzymes, T4 DNA Ligase, Phusion PCR kits and Gibson Assembly Mix from New England BioLabs were used for cloning, and custom oligo DNAs were synthesized by Integrated DNA Technologies (Coralville, IA). .. Genewiz (Cambridge, MA) and Quintara Biosciences (Boston, MA) were used for sequencing analysis.

    Amplification:

    Article Title: A rationally designed nanoparticle for RNA interference therapy in B-lineage lymphoid malignancies
    Article Snippet: .. 2.8 Preparation of a Human Full-length CD22 and CD22ΔE12–14 Plasmids The cDNA fragments encoding either full-length CD22 (CD22FL) or truncated CD22 lacking exons 12–14 (CD22ΔE12–14) were generated by PCR amplification using the Phusion High Fidelity PCR Kit (New England Biolabs, catalog no. E0553L) with the following primer sets: full-length Fwd/Rev 5′-CTTGGT GCTAGC ATGCATCTCCTCGGC-3′/5′CCGG TCTCGAG GATGTTTGAGGATCACATAGTC-3′, and truncation ΔE12–14 Fwd/Rev 5′-CTTGGT GCTAGC ATGCATCTCCTCGGC-3′/5′-CCGGT CTCGAG CCTTTTTATTCCTCAC-3′. .. The correct PCR products (FL: 2541-bp and ΔE12–14: 2208-bp) were ligated into the 8497-bp lentiviral vector pCL6-2AEGwo through the NheI and XhoI restriction sites (underlined) using the Quick Ligase kit (New England Biolabs catalog no. M2200L) following the manufacturer's instructions.

    Article Title: Integrated mapping and characterization of the gene underlying the okra leaf trait in Gossypium hirsutum L
    Article Snippet: .. Sequence comparison The genomic sequences containing the orthologue of Gorai.002G244000 were amplified using LS_F1 and LS_R1 ( Supplementary Table S2 ) from MCU-5, Sicot 71, Coker 315, TM-1, 89004-64 and Siokra 1-4 (G. hirsutum , AD1 ), 3-79, Pima A8, and Sipima 280 (G. barbadense , AD2 ) using a Phusion High-Fidelity PCR kit (New England BioLabs). .. Promoters of GhOKRA in MCU-5 and Siokra 1-4 were amplified using LS_F2 and LS_R2 ( Supplementary Table S2 ).

    Article Title: Impact of genomic polymorphisms on the repertoire of human MHC class I-associated peptides
    Article Snippet: .. PCR and Sanger sequencing PCR amplification of the MiHA-encoding DNA and cDNA regions was performed with the Phusion High-Fidelity PCR kit (New England BioLabs). .. For each candidate, 1–2 pairs of sequencing primers were designed manually and with the PrimerQuest software (Integrated DNA Technologies, ), and were synthesized by Sigma.

    Article Title: Selection of an Efficient AAV Vector for Robust CNS Transgene Expression
    Article Snippet: .. After DNA extraction, the Cap9 inserts (containing the 21-mer sequence encoding the 7-mer peptides) were amplified using the following primers: Cap9_Kpn/Age_For: 5′-AGCTACCGACAACAACGTGT-3′ and Cap9_ Kpn/Age_Rev: 5′- AGAAGGGTGAAAGTTGCCGT-3′ (Phusion high-fidelity PCR kit, New England Biolabs). .. The amplicons were then purified (Monarch PCR and DNA cleanup kit, New England Biolabs), digested by KpnI, AgeI, and BanII, and the Cap9 KpnI-AgeI fragments (144 bp) were agarose gel purified (Monarch DNA gel extraction kit, New England Biolabs) before ligation in the pUC57-Cap9-XbaI/AgeI/KpnI plasmid (opened with KpnI and AgeI and dephosphorylated with calf inositol phosphatase, New England Biolabs).

    Article Title: Cell-Specific Establishment of Poliovirus Resistance to an Inhibitor Targeting a Cellular Protein
    Article Snippet: .. A PCR fragment covering 2C-3A genomic region was amplified using a Phusion PCR kit (New England BioLabs) and sequenced using a commercial sequencing service (Genewiz, Inc.). .. Polio replicon assays were performed essentially as described previously , with minor modifications depending on the cell culture used.

    Article Title: Efficient engineering of marker-free synthetic allotetraploids of Saccharomyces
    Article Snippet: .. Plasmid components were amplified using the Phusion PCR Kit (New England Biolabs, Ipswich, MA) as directed by the product insert. .. Mating-type screening and PCR-RFLP analysis used the Standard Taq Polymerase (New England Biolabs, Ipswich, MA) system as directed by the product insert for amplification.

    Article Title: A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress
    Article Snippet: .. The CgHSP70 ORF, lacking its stop codon, was amplified using a Phusion® High-Fidelity PCR kit (New England Biolabs, Ipswich, MA, USA) with the primer pair CgHSP-Dra-F/-Not-R , and the resulting amplicon cloned into pMD19-T for validation by sequencing. .. The same fragment was also inserted into the Dra I and Not I cloning sites of the pENTR™ 1A dual selection vector (Invitrogen, Carlsbad, CA, USA) using T4 DNA ligase (TaKaRa, Tokyo, Japan), and from there introduced into pEarleyGate 103 using LR Clonase™ II enzyme mix (Invitrogen, Carlsbad, CA, USA) [ ].

    Synthesized:

    Article Title: Creating single-copy genetic circuits
    Article Snippet: .. Restriction enzymes, T4 DNA Ligase, Phusion PCR kits and Gibson Assembly Mix from New England BioLabs were used for cloning, and custom oligo DNAs were synthesized by Integrated DNA Technologies (Coralville, IA). .. Genewiz (Cambridge, MA) and Quintara Biosciences (Boston, MA) were used for sequencing analysis.

    Genomic Sequencing:

    Article Title: Integrated mapping and characterization of the gene underlying the okra leaf trait in Gossypium hirsutum L
    Article Snippet: .. Sequence comparison The genomic sequences containing the orthologue of Gorai.002G244000 were amplified using LS_F1 and LS_R1 ( Supplementary Table S2 ) from MCU-5, Sicot 71, Coker 315, TM-1, 89004-64 and Siokra 1-4 (G. hirsutum , AD1 ), 3-79, Pima A8, and Sipima 280 (G. barbadense , AD2 ) using a Phusion High-Fidelity PCR kit (New England BioLabs). .. Promoters of GhOKRA in MCU-5 and Siokra 1-4 were amplified using LS_F2 and LS_R2 ( Supplementary Table S2 ).

    Sequencing:

    Article Title: Integrated mapping and characterization of the gene underlying the okra leaf trait in Gossypium hirsutum L
    Article Snippet: .. Sequence comparison The genomic sequences containing the orthologue of Gorai.002G244000 were amplified using LS_F1 and LS_R1 ( Supplementary Table S2 ) from MCU-5, Sicot 71, Coker 315, TM-1, 89004-64 and Siokra 1-4 (G. hirsutum , AD1 ), 3-79, Pima A8, and Sipima 280 (G. barbadense , AD2 ) using a Phusion High-Fidelity PCR kit (New England BioLabs). .. Promoters of GhOKRA in MCU-5 and Siokra 1-4 were amplified using LS_F2 and LS_R2 ( Supplementary Table S2 ).

    Article Title: Impact of genomic polymorphisms on the repertoire of human MHC class I-associated peptides
    Article Snippet: .. PCR and Sanger sequencing PCR amplification of the MiHA-encoding DNA and cDNA regions was performed with the Phusion High-Fidelity PCR kit (New England BioLabs). .. For each candidate, 1–2 pairs of sequencing primers were designed manually and with the PrimerQuest software (Integrated DNA Technologies, ), and were synthesized by Sigma.

    Article Title: Selection of an Efficient AAV Vector for Robust CNS Transgene Expression
    Article Snippet: .. After DNA extraction, the Cap9 inserts (containing the 21-mer sequence encoding the 7-mer peptides) were amplified using the following primers: Cap9_Kpn/Age_For: 5′-AGCTACCGACAACAACGTGT-3′ and Cap9_ Kpn/Age_Rev: 5′- AGAAGGGTGAAAGTTGCCGT-3′ (Phusion high-fidelity PCR kit, New England Biolabs). .. The amplicons were then purified (Monarch PCR and DNA cleanup kit, New England Biolabs), digested by KpnI, AgeI, and BanII, and the Cap9 KpnI-AgeI fragments (144 bp) were agarose gel purified (Monarch DNA gel extraction kit, New England Biolabs) before ligation in the pUC57-Cap9-XbaI/AgeI/KpnI plasmid (opened with KpnI and AgeI and dephosphorylated with calf inositol phosphatase, New England Biolabs).

    Article Title: Cell-Specific Establishment of Poliovirus Resistance to an Inhibitor Targeting a Cellular Protein
    Article Snippet: .. A PCR fragment covering 2C-3A genomic region was amplified using a Phusion PCR kit (New England BioLabs) and sequenced using a commercial sequencing service (Genewiz, Inc.). .. Polio replicon assays were performed essentially as described previously , with minor modifications depending on the cell culture used.

    Article Title: A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress
    Article Snippet: .. The CgHSP70 ORF, lacking its stop codon, was amplified using a Phusion® High-Fidelity PCR kit (New England Biolabs, Ipswich, MA, USA) with the primer pair CgHSP-Dra-F/-Not-R , and the resulting amplicon cloned into pMD19-T for validation by sequencing. .. The same fragment was also inserted into the Dra I and Not I cloning sites of the pENTR™ 1A dual selection vector (Invitrogen, Carlsbad, CA, USA) using T4 DNA ligase (TaKaRa, Tokyo, Japan), and from there introduced into pEarleyGate 103 using LR Clonase™ II enzyme mix (Invitrogen, Carlsbad, CA, USA) [ ].

    Generated:

    Article Title: A rationally designed nanoparticle for RNA interference therapy in B-lineage lymphoid malignancies
    Article Snippet: .. 2.8 Preparation of a Human Full-length CD22 and CD22ΔE12–14 Plasmids The cDNA fragments encoding either full-length CD22 (CD22FL) or truncated CD22 lacking exons 12–14 (CD22ΔE12–14) were generated by PCR amplification using the Phusion High Fidelity PCR Kit (New England Biolabs, catalog no. E0553L) with the following primer sets: full-length Fwd/Rev 5′-CTTGGT GCTAGC ATGCATCTCCTCGGC-3′/5′CCGG TCTCGAG GATGTTTGAGGATCACATAGTC-3′, and truncation ΔE12–14 Fwd/Rev 5′-CTTGGT GCTAGC ATGCATCTCCTCGGC-3′/5′-CCGGT CTCGAG CCTTTTTATTCCTCAC-3′. .. The correct PCR products (FL: 2541-bp and ΔE12–14: 2208-bp) were ligated into the 8497-bp lentiviral vector pCL6-2AEGwo through the NheI and XhoI restriction sites (underlined) using the Quick Ligase kit (New England Biolabs catalog no. M2200L) following the manufacturer's instructions.

    Polymerase Chain Reaction:

    Article Title: A rationally designed nanoparticle for RNA interference therapy in B-lineage lymphoid malignancies
    Article Snippet: .. 2.8 Preparation of a Human Full-length CD22 and CD22ΔE12–14 Plasmids The cDNA fragments encoding either full-length CD22 (CD22FL) or truncated CD22 lacking exons 12–14 (CD22ΔE12–14) were generated by PCR amplification using the Phusion High Fidelity PCR Kit (New England Biolabs, catalog no. E0553L) with the following primer sets: full-length Fwd/Rev 5′-CTTGGT GCTAGC ATGCATCTCCTCGGC-3′/5′CCGG TCTCGAG GATGTTTGAGGATCACATAGTC-3′, and truncation ΔE12–14 Fwd/Rev 5′-CTTGGT GCTAGC ATGCATCTCCTCGGC-3′/5′-CCGGT CTCGAG CCTTTTTATTCCTCAC-3′. .. The correct PCR products (FL: 2541-bp and ΔE12–14: 2208-bp) were ligated into the 8497-bp lentiviral vector pCL6-2AEGwo through the NheI and XhoI restriction sites (underlined) using the Quick Ligase kit (New England Biolabs catalog no. M2200L) following the manufacturer's instructions.

    Article Title: Integrated mapping and characterization of the gene underlying the okra leaf trait in Gossypium hirsutum L
    Article Snippet: .. Sequence comparison The genomic sequences containing the orthologue of Gorai.002G244000 were amplified using LS_F1 and LS_R1 ( Supplementary Table S2 ) from MCU-5, Sicot 71, Coker 315, TM-1, 89004-64 and Siokra 1-4 (G. hirsutum , AD1 ), 3-79, Pima A8, and Sipima 280 (G. barbadense , AD2 ) using a Phusion High-Fidelity PCR kit (New England BioLabs). .. Promoters of GhOKRA in MCU-5 and Siokra 1-4 were amplified using LS_F2 and LS_R2 ( Supplementary Table S2 ).

    Article Title: Impact of genomic polymorphisms on the repertoire of human MHC class I-associated peptides
    Article Snippet: .. PCR and Sanger sequencing PCR amplification of the MiHA-encoding DNA and cDNA regions was performed with the Phusion High-Fidelity PCR kit (New England BioLabs). .. For each candidate, 1–2 pairs of sequencing primers were designed manually and with the PrimerQuest software (Integrated DNA Technologies, ), and were synthesized by Sigma.

    Article Title: Selection of an Efficient AAV Vector for Robust CNS Transgene Expression
    Article Snippet: .. After DNA extraction, the Cap9 inserts (containing the 21-mer sequence encoding the 7-mer peptides) were amplified using the following primers: Cap9_Kpn/Age_For: 5′-AGCTACCGACAACAACGTGT-3′ and Cap9_ Kpn/Age_Rev: 5′- AGAAGGGTGAAAGTTGCCGT-3′ (Phusion high-fidelity PCR kit, New England Biolabs). .. The amplicons were then purified (Monarch PCR and DNA cleanup kit, New England Biolabs), digested by KpnI, AgeI, and BanII, and the Cap9 KpnI-AgeI fragments (144 bp) were agarose gel purified (Monarch DNA gel extraction kit, New England Biolabs) before ligation in the pUC57-Cap9-XbaI/AgeI/KpnI plasmid (opened with KpnI and AgeI and dephosphorylated with calf inositol phosphatase, New England Biolabs).

    Article Title: Cell-Specific Establishment of Poliovirus Resistance to an Inhibitor Targeting a Cellular Protein
    Article Snippet: .. A PCR fragment covering 2C-3A genomic region was amplified using a Phusion PCR kit (New England BioLabs) and sequenced using a commercial sequencing service (Genewiz, Inc.). .. Polio replicon assays were performed essentially as described previously , with minor modifications depending on the cell culture used.

    Article Title: Efficient engineering of marker-free synthetic allotetraploids of Saccharomyces
    Article Snippet: .. Plasmid components were amplified using the Phusion PCR Kit (New England Biolabs, Ipswich, MA) as directed by the product insert. .. Mating-type screening and PCR-RFLP analysis used the Standard Taq Polymerase (New England Biolabs, Ipswich, MA) system as directed by the product insert for amplification.

    Article Title: A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress
    Article Snippet: .. The CgHSP70 ORF, lacking its stop codon, was amplified using a Phusion® High-Fidelity PCR kit (New England Biolabs, Ipswich, MA, USA) with the primer pair CgHSP-Dra-F/-Not-R , and the resulting amplicon cloned into pMD19-T for validation by sequencing. .. The same fragment was also inserted into the Dra I and Not I cloning sites of the pENTR™ 1A dual selection vector (Invitrogen, Carlsbad, CA, USA) using T4 DNA ligase (TaKaRa, Tokyo, Japan), and from there introduced into pEarleyGate 103 using LR Clonase™ II enzyme mix (Invitrogen, Carlsbad, CA, USA) [ ].

    Article Title: Creating single-copy genetic circuits
    Article Snippet: .. Restriction enzymes, T4 DNA Ligase, Phusion PCR kits and Gibson Assembly Mix from New England BioLabs were used for cloning, and custom oligo DNAs were synthesized by Integrated DNA Technologies (Coralville, IA). .. Genewiz (Cambridge, MA) and Quintara Biosciences (Boston, MA) were used for sequencing analysis.

    IA:

    Article Title: Creating single-copy genetic circuits
    Article Snippet: .. Restriction enzymes, T4 DNA Ligase, Phusion PCR kits and Gibson Assembly Mix from New England BioLabs were used for cloning, and custom oligo DNAs were synthesized by Integrated DNA Technologies (Coralville, IA). .. Genewiz (Cambridge, MA) and Quintara Biosciences (Boston, MA) were used for sequencing analysis.

    Plasmid Preparation:

    Article Title: Efficient engineering of marker-free synthetic allotetraploids of Saccharomyces
    Article Snippet: .. Plasmid components were amplified using the Phusion PCR Kit (New England Biolabs, Ipswich, MA) as directed by the product insert. .. Mating-type screening and PCR-RFLP analysis used the Standard Taq Polymerase (New England Biolabs, Ipswich, MA) system as directed by the product insert for amplification.

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  • 99
    New England Biolabs phusion high fidelity enzyme
    Phusion High Fidelity Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion high fidelity enzyme/product/New England Biolabs
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    phusion high fidelity enzyme - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    89
    New England Biolabs high fidelity phusion enzyme
    High Fidelity Phusion Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high fidelity phusion enzyme/product/New England Biolabs
    Average 89 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    high fidelity phusion enzyme - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

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