phusion dna polymerase reaction mix  (Thermo Fisher)


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    Structured Review

    Thermo Fisher phusion dna polymerase reaction mix
    Phusion Dna Polymerase Reaction Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion dna polymerase reaction mix/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phusion dna polymerase reaction mix - by Bioz Stars, 2020-03
    86/100 stars

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    Clone Assay:

    Article Title: Human glycosylation enzymes for enzymatic, structural and functional studies
    Article Snippet: Paragraph title: Isolation of protein coding regions and capture as Gateway donor clones ... Test amplifications were performed using respective gene-specific primers, the human cDNAs, and Phusion DNA polymerase reaction mix (ThermoFisher Scientific) as described above.

    Amplification:

    Article Title: Human glycosylation enzymes for enzymatic, structural and functional studies
    Article Snippet: For genes that were not available in MGC or not successfully amplified from MGC clone templates, an alternative amplification approach was employed. .. Test amplifications were performed using respective gene-specific primers, the human cDNAs, and Phusion DNA polymerase reaction mix (ThermoFisher Scientific) as described above.

    Article Title: Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
    Article Snippet: CPEC We obtained linear vectors with PCR amplification and gel purified it using E.Z.N.A gel extraction kits (Omega Bio-Tek). .. 200 ng of the linear vector was mixed with insert DNA at equal molar ratio in a 20 µl volume containing Phusion DNA polymerase reaction mixture (Finnzymes).

    Isolation:

    Article Title: Human glycosylation enzymes for enzymatic, structural and functional studies
    Article Snippet: Paragraph title: Isolation of protein coding regions and capture as Gateway donor clones ... Test amplifications were performed using respective gene-specific primers, the human cDNAs, and Phusion DNA polymerase reaction mix (ThermoFisher Scientific) as described above.

    Purification:

    Article Title: Human glycosylation enzymes for enzymatic, structural and functional studies
    Article Snippet: Test amplifications were performed using respective gene-specific primers, the human cDNAs, and Phusion DNA polymerase reaction mix (ThermoFisher Scientific) as described above. .. Once optimal primary amplification conditions were identified, the resulting PCR products were purified using a QiaQuick PCR purification kit (Qiagen) and subjected to a second round amplification as described above using the universal primers shown in .

    Article Title: Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
    Article Snippet: We added vector-overlapping sequences onto the lacZα gene (with a C-terminal His6 tag) using PCR (see for primer sequences) and gel purified the insert. .. 200 ng of the linear vector was mixed with insert DNA at equal molar ratio in a 20 µl volume containing Phusion DNA polymerase reaction mixture (Finnzymes).

    DNA Sequencing:

    Article Title: Human glycosylation enzymes for enzymatic, structural and functional studies
    Article Snippet: The resulting plasmid clones were screened by restriction mapping and verified by DNA sequencing of the entire coding region. .. Test amplifications were performed using respective gene-specific primers, the human cDNAs, and Phusion DNA polymerase reaction mix (ThermoFisher Scientific) as described above.

    Polymerase Chain Reaction:

    Article Title: Human glycosylation enzymes for enzymatic, structural and functional studies
    Article Snippet: PCR products were then used directly for Gateway recombination with the pDONR221 vector in BP Clonase II reactions with a final volume of 10 μl, as described by the manufacturer (ThermoFisher Scientific). .. Test amplifications were performed using respective gene-specific primers, the human cDNAs, and Phusion DNA polymerase reaction mix (ThermoFisher Scientific) as described above.

    Article Title: Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
    Article Snippet: We added vector-overlapping sequences onto the lacZα gene (with a C-terminal His6 tag) using PCR (see for primer sequences) and gel purified the insert. .. 200 ng of the linear vector was mixed with insert DNA at equal molar ratio in a 20 µl volume containing Phusion DNA polymerase reaction mixture (Finnzymes).

    Gel Extraction:

    Article Title: Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
    Article Snippet: CPEC We obtained linear vectors with PCR amplification and gel purified it using E.Z.N.A gel extraction kits (Omega Bio-Tek). .. 200 ng of the linear vector was mixed with insert DNA at equal molar ratio in a 20 µl volume containing Phusion DNA polymerase reaction mixture (Finnzymes).

    Transformation Assay:

    Article Title: Human glycosylation enzymes for enzymatic, structural and functional studies
    Article Snippet: The reaction products were transformed into the 5-alpha competent E. coli strain (New England Biolabs, Ipswich, MA), and plated on LB plates containing kanamycin. .. Test amplifications were performed using respective gene-specific primers, the human cDNAs, and Phusion DNA polymerase reaction mix (ThermoFisher Scientific) as described above.

    Plasmid Preparation:

    Article Title: Human glycosylation enzymes for enzymatic, structural and functional studies
    Article Snippet: The resulting plasmid clones were screened by restriction mapping and verified by DNA sequencing of the entire coding region. .. Test amplifications were performed using respective gene-specific primers, the human cDNAs, and Phusion DNA polymerase reaction mix (ThermoFisher Scientific) as described above.

    Article Title: Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
    Article Snippet: .. 200 ng of the linear vector was mixed with insert DNA at equal molar ratio in a 20 µl volume containing Phusion DNA polymerase reaction mixture (Finnzymes). .. We denatured the insert and vector mixture at 98°C for 30 seconds, annealed them at 55°C for 30 seconds, and performed polymerase extension for 15 seconds per kb according to the length of the longest piece.

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