Journal: mSphere
Article Title: Temporal, Environmental, and Biological Drivers of the Mucosal Microbiome in a Wild Marine Fish, Scomber japonicus
doi: 10.1128/mSphere.00401-20
Figure Lengend Snippet: Prevalence of marine vertebrate pathogen Photobacterium damselae on S. japonicus body sites throughout the sampling effort. (a) Validation of P. damselae 148-bp v4 region by way of phylogenetic comparison to two known pathogenic isolates and nonpathogenic strains. (b) Total relative abundance of P. damselae sOTU across five body sites and environments for successfully sequenced samples. Total prevalence or percentage of samples with P. damelsae present is also calculated for each sample type and displayed at the top of graph (7.4% gill samples, 16.1% skin, 14.7% digesta, 22.2% GI, 5.6% pyloric ceca, 0% water, and 10.5% sediment). (c and d) Proportion of microbial community comprised of P. damselae sOTU across the most prevalent body sites (skin, digesta, and GI) over the 38 sampling events (c) or binned by month across 1 year (d). Relative abundance is calculated as number of P. damselae sOTU reads divided by 1,362, the rarefaction number. Any samples with 0 P. damselae reads are considered under the detection limit and are displayed as equal to 0.00001 relative abundance in order to visualize on the log scale.
Article Snippet: The comparison bacteria strains included the following: two pathogenic Photobacterium species isolates ( P. damselae ATCC 33539 T , GenBank accession no. X74700.1 ; P. damselae , GenBank accession no. D25308.1 ), four nonpathogenic Photobacterium spp. ( P. leiognathi strain ATCC 25521, GenBank accession no. NR_115541.1 ; P. angustum ATCC 25915 T , GenBank accession no. X74685.1 ; P. phosphoreum strain ATCC 11040, GenBank accession no. NR_115205.1 ; P. rosenbergii strain CC1, GenBank accession no. NR_042343.1 ), and two outgroup Vibrio species ( Vibrio pelagius strain ATCC 25916, GenBank accession no. NR_119059.1 ; Aliivibrio fischeri strain ATCC 7744, GenBank accession no. NR_115204.1 ) which were identified from various studies ( ).
Techniques: Sampling