Journal: Preventive Nutrition and Food Science
Article Title: Standardized Fingerroot ( Boesenbergia pandurata ) Extract Decelerates the Development of Sarcopenia in Aged Rats
doi: 10.3746/pnf.2025.30.1.47
Figure Lengend Snippet: Effects of Boesenbergia pandurata extract (BPE) on protein-degradation-related factors. (A) MuRF1 and atrogin-1 mRNA expression. Values were normalized to the expression of β-actin. (B) p-FoxO3a and FoxO3a protein expression levels. Values were normalized to the expression of the total form of FoxO3a. β-Actin and α-tubulin were used as loading controls. Total RNA and protein were isolated from gastrocnemius muscle tissues. Values are presented as the mean±SD (n=4 per group). # P <0.05, ## P <0.01 (vs. Young group); * P <0.05, ** P <0.01 (vs. Aged group).
Article Snippet: After washing with in Tris-buffered saline containing Tween 20 (TBST), the membranes were incubated with primary antibodies against FoxO3a, phosphorylated (p)-FoxO3a Thr32, PI3K, p-PI3K Tyr458, Akt, p-Akt Ser473, mTOR, p-mTOR Ser2448, p70S6K, p-p70S6K Thr389, 4EBP1, p-4EBP1 Thr37/46, and α-tubulin (1:1,000 dilution; Cell Signaling) overnight at 4°C.
Techniques: Expressing, Isolation