phosphorylated p erk 1 2  (Cell Signaling Technology Inc)

Journal: Antioxidants

Article Title: Dietary Curcumin Attenuates Hepatic Cellular Senescence by Suppressing the MAPK/NF-κB Signaling Pathway in Aged Mice

doi: 10.3390/antiox12061165

Figure Lengend Snippet: Curcumin ameliorates hepatic cellular senescence by regulating the p38-MAPK signaling pathway in HFHSD-fed aged mice. Immunoblots and protein expression levels in the liver tissue lysates after 15 weeks of dietary interventions: ( A ) p-ERK1/2, ERK1/2, and p-ERK/ERK ratio; ( B ) p-JNK, JNK, and p-JNK/JNK ratio; ( C ) p-p38, p38, and p-p38/p38 ratio; and ( D ) p-MK2, MK2, and p-MK2/MK2 ratio. The results are expressed as mean ± SEM (* p < 0.05).

Article Snippet: The membranes were blocked in a blocking reagent (LI-COR, Lincoln, NE, USA) at room temperature for 1 h and incubated with the primary antibodies at 4 °C overnight as follows: phosphorylated extracellular signal-regulated kinase (p-ERK) 1/2, ERK1/2, phosphorylated c-Jun N-terminal kinase (p-JNK) 1/2, JNK1/2, p-p38, p38, phosphorylated MAP kinase-activated protein kinase 2 (p-MK2), MK2, p-p65, p65, p53, cyclophilin B, histone deacetylase 1 (HDAC1), β-tubulin, and β-actin from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Western Blot, Expressing