phosphorylated p erk  (Proteintech)

Journal: Journal of Inflammation Research

Article Title: Myrrh Essential Oil Improves DSS-Induced Colitis by Modulating the MAPK Signaling Pathway: In vitro and in vivo Studies

doi: 10.2147/JIR.S473596

Figure Lengend Snippet: The Results Immunohistochemistry. ( A ) Effect of MEO on the levels of p-JNK, p-ERK, and TNF-α protein in DSS-induced colon tissues. ( B ) Protein levels of p-JNK in colon tissues of each mice group. ( C ) Protein levels of P-ERK in colon tissues of each mice group. ( D ) Protein levels of TNF-α in colon tissues of each mice group.

Article Snippet: Electrophoresis was performed on a sodium dodecyl sulfate-polyacrylamide gel, and at the end of the gel, the proteins were transferred to a PVDF membrane and blocked with 5% skim milk for 1 h. Added the Primary antibodies phosphorylated amino-terminal protein kinase (p-JNK) (Triple Eagle, 1:1000), c-Jun amino-terminal kinase (JNK) (Triple Eagle, 1:3000), phosphorylated extracellular signal-regulated kinase (p-ERK) (Saville, 1:400), and extracellular signal-regulated kinase (ERK) (Abcam, 1:1000), and the internal reference glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Huaan Bio, 1:5000) then incubated overnight at 4 °C.

Techniques: Immunohistochemistry

Journal: Journal of Inflammation Research

Article Title: Myrrh Essential Oil Improves DSS-Induced Colitis by Modulating the MAPK Signaling Pathway: In vitro and in vivo Studies

doi: 10.2147/JIR.S473596

Figure Lengend Snippet: The results of Western blot. ( A ) Changes in the levels of P-JNK, JNK, P-ERK, and ERK in DSS-induced colon tissues after treatment with MEO. ( B ) Protein levels of p-JNK/JNK in colon tissues of each mice group. ( C ) Protein levels of p-ERK/ERK in colon tissues of each mice group.

Article Snippet: Electrophoresis was performed on a sodium dodecyl sulfate-polyacrylamide gel, and at the end of the gel, the proteins were transferred to a PVDF membrane and blocked with 5% skim milk for 1 h. Added the Primary antibodies phosphorylated amino-terminal protein kinase (p-JNK) (Triple Eagle, 1:1000), c-Jun amino-terminal kinase (JNK) (Triple Eagle, 1:3000), phosphorylated extracellular signal-regulated kinase (p-ERK) (Saville, 1:400), and extracellular signal-regulated kinase (ERK) (Abcam, 1:1000), and the internal reference glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Huaan Bio, 1:5000) then incubated overnight at 4 °C.

Techniques: Western Blot