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Curcumin modulates the AKT-Raf1-ERK1/2 signaling pathway in chicken granulosa cells under oxidative stress. (A) Protein expression of <t>AKT1,</t> Raf1, and ERK1/2 under oxidative stress and curcumin treatment. (B) Relative expression of AKT. (C) Relative expression of p-AKT. (D-E) Changes in the relative expression and phosphorylation levels of Raf1. (F-G) Changes in the relative expression and phosphorylation levels of ERK1/2. The results are presented in the form of mean ± SD. n = 4. * p < 0.05; ** p < 0.01; **** p < 0.0001; ns p ≥ 0.05.
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Curcumin modulates the AKT-Raf1-ERK1/2 signaling pathway in chicken granulosa cells under oxidative stress. (A) Protein expression of AKT1, Raf1, and ERK1/2 under oxidative stress and curcumin treatment. (B) Relative expression of AKT. (C) Relative expression of p-AKT. (D-E) Changes in the relative expression and phosphorylation levels of Raf1. (F-G) Changes in the relative expression and phosphorylation levels of ERK1/2. The results are presented in the form of mean ± SD. n = 4. * p < 0.05; ** p < 0.01; **** p < 0.0001; ns p ≥ 0.05.

Journal: Poultry Science

Article Title: Curcumin alleviates proliferation dysfunction in chicken granulosa cells under oxidative stress through AKT-Raf1-ERK1/2 signaling pathway

doi: 10.1016/j.psj.2025.105342

Figure Lengend Snippet: Curcumin modulates the AKT-Raf1-ERK1/2 signaling pathway in chicken granulosa cells under oxidative stress. (A) Protein expression of AKT1, Raf1, and ERK1/2 under oxidative stress and curcumin treatment. (B) Relative expression of AKT. (C) Relative expression of p-AKT. (D-E) Changes in the relative expression and phosphorylation levels of Raf1. (F-G) Changes in the relative expression and phosphorylation levels of ERK1/2. The results are presented in the form of mean ± SD. n = 4. * p < 0.05; ** p < 0.01; **** p < 0.0001; ns p ≥ 0.05.

Article Snippet: ABclonal , Phospho-AKT1 (Ser473) Rabbit pAb , 1: 1000.

Techniques: Expressing, Phospho-proteomics