Journal: Neural Regeneration Research

Article Title: Subretinal fibrosis secondary to neovascular age-related macular degeneration: mechanisms and potential therapeutic targets

doi: 10.4103/NRR.NRR-D-23-01642

Figure Lengend Snippet: Risk and protective factors for subretinal fibrosis in neovascular age-related macular degeneration

Article Snippet: Pro-fibrotic , Transforming growth factor-β signaling pathway Wnt signaling pathway Phosphatidylinositol-3-kinase/Akt pathway Vascular endothelial growth factor/vascular endothelial growth factor receptor Platelet derived growth factor/platelet derived growth factor receptor-β Interleukin-6/interleukin-6 receptor Hypoxia-inducible factor-1α/p53/miRNA-34a/Klotho pathway MRTF-A-SRF pathway Connective tissue growth factor, fibroblast growth factor 2, platelet-activating factor receptor, Galectin-1, Yes-associated protein, adiponectin, methyltransferase-like 3, matrix metalloproteinase 12, peptidyl arginine deiminase-4, αB-Crystallin, Snail (SNIA1), cyclooxygenase-2, (pro)renin receptor, sphingosine-1-phosphate, γ-secretase.

Techniques: Injection

Journal: Neural Regeneration Research

Article Title: Subretinal fibrosis secondary to neovascular age-related macular degeneration: mechanisms and potential therapeutic targets

doi: 10.4103/NRR.NRR-D-23-01642

Figure Lengend Snippet: Schematic illustrations for animal models of subretinal fibrosis. Several subretinal fibrosis animal models can be created using laser photocoagulation, subretinal injection of macrophage-rich peritoneal exudate cells, or subretinal injection of Matrigel. Created with BioRender.com. C3: Complement component 3; CFB: complement component factor B; CNV: choroidal neovascularization; FGF2: fibroblast growth factor 2; iNOS: inducible nitric oxide synthase; PBS: phosphate-buffered saline; PECs: peritoneal exudate cells; TGFβ: transforming growth factor-β; VEGF: vascular endothelial growth factor.

Article Snippet: Pro-fibrotic , Transforming growth factor-β signaling pathway Wnt signaling pathway Phosphatidylinositol-3-kinase/Akt pathway Vascular endothelial growth factor/vascular endothelial growth factor receptor Platelet derived growth factor/platelet derived growth factor receptor-β Interleukin-6/interleukin-6 receptor Hypoxia-inducible factor-1α/p53/miRNA-34a/Klotho pathway MRTF-A-SRF pathway Connective tissue growth factor, fibroblast growth factor 2, platelet-activating factor receptor, Galectin-1, Yes-associated protein, adiponectin, methyltransferase-like 3, matrix metalloproteinase 12, peptidyl arginine deiminase-4, αB-Crystallin, Snail (SNIA1), cyclooxygenase-2, (pro)renin receptor, sphingosine-1-phosphate, γ-secretase.

Techniques: Injection, Saline

Journal: Neural Regeneration Research

Article Title: Subretinal fibrosis secondary to neovascular age-related macular degeneration: mechanisms and potential therapeutic targets

doi: 10.4103/NRR.NRR-D-23-01642

Figure Lengend Snippet: Pathways and molecules involved in the pathogenesis of subretinal fibrosis

Article Snippet: Pro-fibrotic , Transforming growth factor-β signaling pathway Wnt signaling pathway Phosphatidylinositol-3-kinase/Akt pathway Vascular endothelial growth factor/vascular endothelial growth factor receptor Platelet derived growth factor/platelet derived growth factor receptor-β Interleukin-6/interleukin-6 receptor Hypoxia-inducible factor-1α/p53/miRNA-34a/Klotho pathway MRTF-A-SRF pathway Connective tissue growth factor, fibroblast growth factor 2, platelet-activating factor receptor, Galectin-1, Yes-associated protein, adiponectin, methyltransferase-like 3, matrix metalloproteinase 12, peptidyl arginine deiminase-4, αB-Crystallin, Snail (SNIA1), cyclooxygenase-2, (pro)renin receptor, sphingosine-1-phosphate, γ-secretase.

Techniques: Derivative Assay

Journal: Scientific Reports

Article Title: Relationship between inherited genetic variation and survival from colorectal cancer stratified by tumour location

doi: 10.1038/s41598-024-77870-0

Figure Lengend Snippet: Replication of loci suggestive of association with survival in COIN and COIN-B.

Article Snippet: We investigated the relationship between Phosphatidylinositol 4-Kinase Type 2 Beta ( PI4K2B ) expression in colorectal tumours and survival in 597 patients from The Human Protein Atlas (THPA).

Techniques:

Journal: Scientific Reports

Article Title: Relationship between inherited genetic variation and survival from colorectal cancer stratified by tumour location

doi: 10.1038/s41598-024-77870-0

Figure Lengend Snippet: Relationship between gene, genotype and survival in patients from COIN and COIN-B with primary tumours in the distal colon. ( A ) Manhattan plot of gene associations with survival. Genes are ordered by chromosome position and plotted against the −log 10 ( P ) for their association with survival. The red line represents the threshold for genome-wide significance ( P = 2.5 × 10 –6 ). ( B ) Regional locus zoom plot shows results of the analysis for single nucleotide polymorphisms (SNPs) and recombination rates. − log 10 ( P ) (y axis) of the SNPs are shown according to their chromosomal positions (x axis) for an area 200 Kb upstream and downstream of PI4K2B . The sentinel SNP (purple) is labelled by its rsID (rs313566). The colour intensity of each symbol reflects the extent of linkage disequilibrium with the sentinel SNP, deep blue (r 2 = 0) through to dark red (r 2 = 1.0). Genetic recombination rates, estimated using 1000 Genomes Project samples, are shown with a blue line. Physical positions are based on NCBI build 37 of the human genome. Also shown are the relative positions of genes and transcripts mapping to the region of association. Genes have been redrawn to show their relative positions; therefore, maps are not to physical scale. ( C ) Kaplan–Meier plot of the relationship between rs313566 genotype and survival. Time in days plotted against survival probability for patients homozygous for the major allele (GG) and heterozygous (GA) or homozygous for the minor allele (AA). The number of patients still at risk at each time point is shown beneath.

Article Snippet: We investigated the relationship between Phosphatidylinositol 4-Kinase Type 2 Beta ( PI4K2B ) expression in colorectal tumours and survival in 597 patients from The Human Protein Atlas (THPA).

Techniques: Genome Wide

Journal: Cancer & Metabolism

Article Title: Phosphatidylinositol promoted the proliferation and invasion of pituitary adenoma cells by regulating POU1F1 expression

doi: 10.1186/s40170-024-00372-0

Figure Lengend Snippet: Proteomic analysis of phosphatidylinositol stimulated GH3 cells. A Cluster analysis of differential proteins betweenGH3 cellsandGH3 cells-treated with phosphatidylinositol. B Volcanic map of differential proteins betweenGH3 cellsandGH3 cells-treated with phosphatidylinositol. C Venn diagram of differential proteins from IPA tissues and GH3 cells-treated with phosphatidylinositol

Article Snippet: After washed with PBS, the cells were cultured in complete medium containing 100 μg/ml phosphatidylinositol for 72 h. Images were captured using the olympus microscope (IX53).

Techniques:

Journal: Cancer & Metabolism

Article Title: Phosphatidylinositol promoted the proliferation and invasion of pituitary adenoma cells by regulating POU1F1 expression

doi: 10.1186/s40170-024-00372-0

Figure Lengend Snippet: The effect of phosphatidylinositol on the proliferation of invasion of pituitary adenoma cells cells and expression of PITPNM1, POU1F1 and C2orf15. A The expression of PITPNM1, POU1F1, C2orf15 and LDHA in IPA and NIPA tissues. B The primary pituitary adenoma cells (PPAC) and GH3 cells were exposed to the different concentration of phosphatidylinositol for 72 h. CCK8 assay was performed to determine the cell viability. C GH3 cells and PPAC were treated with 100 μg/ml phosphatidylinositol for indicated time. CCK8 assay was performed to determine the cell viability. D Scratch assay was performed to determine the effect of phosphatidylinositol on cell migration. E Transwell assay was performed to determine the effect of phosphatidylinositol on cell invasion. F After treatment with phosphatidylinositol, western blot analysis was performed to determine the expression of PITPNM1, POU1F1, C2orf15 and LDHA. ***, p < 0.001

Article Snippet: After washed with PBS, the cells were cultured in complete medium containing 100 μg/ml phosphatidylinositol for 72 h. Images were captured using the olympus microscope (IX53).

Techniques: Expressing, Concentration Assay, CCK-8 Assay, Wound Healing Assay, Migration, Transwell Assay, Western Blot

Journal: Cancer & Metabolism

Article Title: Phosphatidylinositol promoted the proliferation and invasion of pituitary adenoma cells by regulating POU1F1 expression

doi: 10.1186/s40170-024-00372-0

Figure Lengend Snippet: The putative mechanism of phosphatidylinositol-mediated in pituitary tumor cells invasion

Article Snippet: After washed with PBS, the cells were cultured in complete medium containing 100 μg/ml phosphatidylinositol for 72 h. Images were captured using the olympus microscope (IX53).

Techniques: