phosphate buffered solution pbs  (Thermo Fisher)


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    Structured Review

    Thermo Fisher phosphate buffered solution pbs
    Comparison of the Raman spectra of D. discoideum growth and starvation <t>EVs.</t> (A) Proposed interpretation of Raman spectrum originated from vesicles released from D. discoideum in the starvation phase. This spectrum was obtained by averaging 15 different sets of vesicles, with a total accumulation time of 14 minutes, then subtraction of the <t>PBS</t> contribution, and smoothing by 2 adjacent pixels. (B) Variability of Raman spectra of D. discoideum vesicles during growth (a, b) as compared to starvation phase (c, d). Within the same phase, one can qualitatively distinguish at least 2 different species: during growth, species (b) is characterised by an increased amount of lipids as compared to species (a), and during starvation, species (d) contains an increased amount of nucleic acids and carotenoids as compared to species (c). The prominent spectral differences are highlighted by dashed oval curves.
    Phosphate Buffered Solution Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered solution pbs/product/Thermo Fisher
    Average 99 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered solution pbs - by Bioz Stars, 2020-12
    99/100 stars

    Images

    1) Product Images from "Fast characterisation of cell-derived extracellular vesicles by nanoparticles tracking analysis, cryo-electron microscopy, and Raman tweezers microspectroscopy"

    Article Title: Fast characterisation of cell-derived extracellular vesicles by nanoparticles tracking analysis, cryo-electron microscopy, and Raman tweezers microspectroscopy

    Journal: Journal of Extracellular Vesicles

    doi: 10.3402/jev.v1i0.19179

    Comparison of the Raman spectra of D. discoideum growth and starvation EVs. (A) Proposed interpretation of Raman spectrum originated from vesicles released from D. discoideum in the starvation phase. This spectrum was obtained by averaging 15 different sets of vesicles, with a total accumulation time of 14 minutes, then subtraction of the PBS contribution, and smoothing by 2 adjacent pixels. (B) Variability of Raman spectra of D. discoideum vesicles during growth (a, b) as compared to starvation phase (c, d). Within the same phase, one can qualitatively distinguish at least 2 different species: during growth, species (b) is characterised by an increased amount of lipids as compared to species (a), and during starvation, species (d) contains an increased amount of nucleic acids and carotenoids as compared to species (c). The prominent spectral differences are highlighted by dashed oval curves.
    Figure Legend Snippet: Comparison of the Raman spectra of D. discoideum growth and starvation EVs. (A) Proposed interpretation of Raman spectrum originated from vesicles released from D. discoideum in the starvation phase. This spectrum was obtained by averaging 15 different sets of vesicles, with a total accumulation time of 14 minutes, then subtraction of the PBS contribution, and smoothing by 2 adjacent pixels. (B) Variability of Raman spectra of D. discoideum vesicles during growth (a, b) as compared to starvation phase (c, d). Within the same phase, one can qualitatively distinguish at least 2 different species: during growth, species (b) is characterised by an increased amount of lipids as compared to species (a), and during starvation, species (d) contains an increased amount of nucleic acids and carotenoids as compared to species (c). The prominent spectral differences are highlighted by dashed oval curves.

    Techniques Used:

    Size distribution and concentration of D. discoideum EVs samples ( Table I ), as measured by NTA. EVs were prepared from growth medium after 48 hours of cell growth, either with the usual conditions for the 12,000×g centrifugation (in Eppendorf tubes) (a), or with the 12,000×g centrifugation performed in 30 ml tubes (see Materials and methods ) (b). The curve (c) corresponds to EVs remaining in the 12,000×g supernatant, which relates to sample (b) EVs. The 12,000×g pellets (a and b) are concentrated in PBS by factors of 20 and 100, respectively, whereas the 12,000×g supernatant (c) is as obtained after centrifugation.
    Figure Legend Snippet: Size distribution and concentration of D. discoideum EVs samples ( Table I ), as measured by NTA. EVs were prepared from growth medium after 48 hours of cell growth, either with the usual conditions for the 12,000×g centrifugation (in Eppendorf tubes) (a), or with the 12,000×g centrifugation performed in 30 ml tubes (see Materials and methods ) (b). The curve (c) corresponds to EVs remaining in the 12,000×g supernatant, which relates to sample (b) EVs. The 12,000×g pellets (a and b) are concentrated in PBS by factors of 20 and 100, respectively, whereas the 12,000×g supernatant (c) is as obtained after centrifugation.

    Techniques Used: Concentration Assay, Centrifugation

    Related Articles

    Cell Culture:

    Article Title: Rescue of cardiomyopathy through U7snRNA-mediated exon skipping in Mybpc3-targeted knock-in mice
    Article Snippet: .. Western blot analysis Crude protein extract was obtained from 50 mg ventricular tissue or from one well (12-well-plate) of cultured NMCMs or HEK293 cells, which was rinsed once with ice-cold 1x D-PBS (Life Technologies). .. Proteins were extracted in lysis buffer (30 mM Tris base pH 8.8, 5 mM EDTA, 30 mM NaF, 3% SDS, 10% glycerol).

    Mouse Assay:

    Article Title: Zinc Acetate/Carrageenan Gels Exhibit Potent Activity In Vivo against High-Dose Herpes Simplex Virus 2 Vaginal and Rectal Challenge
    Article Snippet: .. Mice received subcutaneously 100 μl of medroxyprogesterone acetate (Depo-Provera; Upjohn, Kalamazoo, MI) at 25 mg/ml in D-PBS (Invitrogen). ..

    Concentration Assay:

    Article Title: Fast characterisation of cell-derived extracellular vesicles by nanoparticles tracking analysis, cryo-electron microscopy, and Raman tweezers microspectroscopy
    Article Snippet: .. EVs, present in the 12,000×g pellet, are resuspended in a phosphate-buffered solution (PBS) [pH 7.4 without calcium and magnesium (Gibco)], with a 20 to 100 volume concentration factor (X), relative to the volume of the 2,000×g supernatant. .. The 700×g and 2,000×g centrifugations are performed in single-use 15 ml tubes on an Eppendorf 5804R centrifuge.

    Incubation:

    Article Title: Sub-Nucleocapsid Nanoparticles: A Nasal Vaccine against Respiratory Syncytial Virus
    Article Snippet: .. RAW and D2SC/1 cells were incubated in D-PBS (Gibco) supplemented with 5 mM EDTA, 0.33% lidocaine (Astra Zeneca) for 2 min at 37°C and centrifuged for 7 min at 500 g. The pellet was resuspended in PBS supplemented with 2% FCS. .. Viable cells were adjusted to 107 cells/mL and incubated in round bottom tubes with 30 µM GFP or N-GFP SRS for one hour at 37°C together with propidium iodide at a final concentration of 1 µg/ml.

    other:

    Article Title: Identification of Personal Lubricants That Can Cause Rectal Epithelial Cell Damage and Enhance HIV Type 1 Replication in Vitro
    Article Snippet: Additionally we used Carraguard (Population Council, New York, NY), D-PBS (Invitrogen, Grand Island, NY), and Gynol II Vaginal Contraceptive Jelly (Caldwell Consumer Health, LLC, Parsippany, NJ).

    Western Blot:

    Article Title: Rescue of cardiomyopathy through U7snRNA-mediated exon skipping in Mybpc3-targeted knock-in mice
    Article Snippet: .. Western blot analysis Crude protein extract was obtained from 50 mg ventricular tissue or from one well (12-well-plate) of cultured NMCMs or HEK293 cells, which was rinsed once with ice-cold 1x D-PBS (Life Technologies). .. Proteins were extracted in lysis buffer (30 mM Tris base pH 8.8, 5 mM EDTA, 30 mM NaF, 3% SDS, 10% glycerol).

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