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Staphylococcus aureus lysate obtained after phage infection has no effect on exocytosis of primary and secondary granules from neutrophils. Exocytosis of primary and secondary granules was evaluated based on changes of the expression of CD63 (primary granules) and CD66b (secondary granules) on neutrophils. S. aureus lysate at the titer range 10 6 –10 8 <t>pfu/mL,</t> <t>PBS</t> (the negative control), peptone water (PW; additional control for lysate), or suspension of heat-inactivated S. aureus cells (10 8 cells/mL; the positive control) were added to whole blood samples. After 20-min incubation the percentage of CD63+ neutrophils ( A ), the mean fluorescence intensity (MFI) values of CD63 in the gated neutrophil population ( B ), the percentage of CD66b+ neutrophils ( C ), and the MFI values of CD66b in the gated neutrophil population ( D ) were determined by flow cytometry. The results shown are the mean percentage values of CD63+ and CD66+ neutrophils, as well as the MFI values of CD63 and CD66b in the gated neutrophil population ± SE in individual groups. Statistically significant differences ( p
Pbs, supplied by BioMed Diagnostics Inc, used in various techniques. Bioz Stars score: 92/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: A3R Phage and Staphylococcus aureus Lysate Do Not Induce Neutrophil Degranulation

Journal: Viruses

doi: 10.3390/v9020036

Staphylococcus aureus lysate obtained after phage infection has no effect on exocytosis of primary and secondary granules from neutrophils. Exocytosis of primary and secondary granules was evaluated based on changes of the expression of CD63 (primary granules) and CD66b (secondary granules) on neutrophils. S. aureus lysate at the titer range 10 6 –10 8 pfu/mL, PBS (the negative control), peptone water (PW; additional control for lysate), or suspension of heat-inactivated S. aureus cells (10 8 cells/mL; the positive control) were added to whole blood samples. After 20-min incubation the percentage of CD63+ neutrophils ( A ), the mean fluorescence intensity (MFI) values of CD63 in the gated neutrophil population ( B ), the percentage of CD66b+ neutrophils ( C ), and the MFI values of CD66b in the gated neutrophil population ( D ) were determined by flow cytometry. The results shown are the mean percentage values of CD63+ and CD66+ neutrophils, as well as the MFI values of CD63 and CD66b in the gated neutrophil population ± SE in individual groups. Statistically significant differences ( p
Figure Legend Snippet: Staphylococcus aureus lysate obtained after phage infection has no effect on exocytosis of primary and secondary granules from neutrophils. Exocytosis of primary and secondary granules was evaluated based on changes of the expression of CD63 (primary granules) and CD66b (secondary granules) on neutrophils. S. aureus lysate at the titer range 10 6 –10 8 pfu/mL, PBS (the negative control), peptone water (PW; additional control for lysate), or suspension of heat-inactivated S. aureus cells (10 8 cells/mL; the positive control) were added to whole blood samples. After 20-min incubation the percentage of CD63+ neutrophils ( A ), the mean fluorescence intensity (MFI) values of CD63 in the gated neutrophil population ( B ), the percentage of CD66b+ neutrophils ( C ), and the MFI values of CD66b in the gated neutrophil population ( D ) were determined by flow cytometry. The results shown are the mean percentage values of CD63+ and CD66+ neutrophils, as well as the MFI values of CD63 and CD66b in the gated neutrophil population ± SE in individual groups. Statistically significant differences ( p

Techniques Used: Infection, Expressing, Negative Control, Positive Control, Incubation, Fluorescence, Flow Cytometry, Cytometry

A3R phage does not induce neutrophil degranulation. Neutrophil degranulation was evaluated based on changes of the expression of CD63 (primary granules) and CD66b (secondary granules) on neutrophils. A3R at the titer range 10 6 –10 8 pfu/mL, phosphate-buffered saline (PBS) (the negative control), or suspension of heat-inactivated S. aureus cells (10 8 cells/mL; the positive control) were added to whole blood samples. After 20-min incubation the percentage of CD63 + neutrophils ( A ), the mean fluorescence intensity (MFI) values of CD63 in the gated neutrophil population ( B ), the percentage of CD66b + neutrophils ( C ), and the MFI values of CD66b in the gated neutrophil population ( D ) were determined by flow cytometry. The results shown are the mean percentage values of CD63 + and CD66 + neutrophils, as well as the MFI values of CD63 and CD66b in the gated neutrophil population ± SE in individual groups. Statistically significant differences ( p
Figure Legend Snippet: A3R phage does not induce neutrophil degranulation. Neutrophil degranulation was evaluated based on changes of the expression of CD63 (primary granules) and CD66b (secondary granules) on neutrophils. A3R at the titer range 10 6 –10 8 pfu/mL, phosphate-buffered saline (PBS) (the negative control), or suspension of heat-inactivated S. aureus cells (10 8 cells/mL; the positive control) were added to whole blood samples. After 20-min incubation the percentage of CD63 + neutrophils ( A ), the mean fluorescence intensity (MFI) values of CD63 in the gated neutrophil population ( B ), the percentage of CD66b + neutrophils ( C ), and the MFI values of CD66b in the gated neutrophil population ( D ) were determined by flow cytometry. The results shown are the mean percentage values of CD63 + and CD66 + neutrophils, as well as the MFI values of CD63 and CD66b in the gated neutrophil population ± SE in individual groups. Statistically significant differences ( p

Techniques Used: Expressing, Negative Control, Positive Control, Incubation, Fluorescence, Flow Cytometry, Cytometry

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