phosphatase inhibitor mixture  (Thermo Fisher)


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    Name:
    Halt Phosphatase Inhibitor Cocktail
    Description:
    Thermo Scientific Halt Phosphatase Inhibitor Cocktail preserves the phosphorylation state of proteins during and after cell lysis or tissue protein extraction Features of Halt Phosphatase Inhibitor Cocktail • Devoid of protease inhibitors To protect against both proteases and phosphatases use Halt Protease and Phosphatase Inhibitor Cocktail • Dialyzable Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components • Supplied as a 100X concentrate effective when used at a final concentration of 1X however samples that contain particularly high levels of phosphatase activity may require a final concentration of 2 3X • Compatibility compatible with most detergent based cell lysis reagents including all Thermo Scientific Pierce Cell Lysis Solutions • All in one format inhibits both serine threonine phosphatases and protein tyrosine phosphatases • Compatiblewith popular protein assays works with standard protein assays including BCA and coomassie Bradford assays • Convenient storage refrigerator 2 to 8°C storage saves valuable space in the freezer and provides for immediate use • Multiple sizes select a package size of the 100X cocktail that is ideal for any particular scale and workflow of protein extraction experiment Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine threonine phosphatases as well as protein tyrosine phosphatases PTPs during cell lysis The cocktail contains a mixture of four inhibitors of broad specificity including sodium fluoride sodium orthovanadate sodium pyrophosphate and beta glycerophosphate Conveniently packaged in a 100X format the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays Phosphorylation and dephosphorylation comprise a molecular on off switch that regulates many key biological pathways within the cell including signal transduction cell division and apoptosis To effectively study phosphorylation activation states one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis Halt Phosphatase Inhibitor Cocktail accomplishes this task Related Products Pierce Phosphatase Inhibitor Mini Tablets
    Catalog Number:
    78420
    Price:
    None
    Applications:
    Cell Lysis & Fractionation|Protease and Phosphatase Inhibition|Protein Biology|Protein Purification & Isolation
    Category:
    Lab Reagents and Chemicals
    Buy from Supplier


    Structured Review

    Thermo Fisher phosphatase inhibitor mixture
    Thermo Scientific Halt Phosphatase Inhibitor Cocktail preserves the phosphorylation state of proteins during and after cell lysis or tissue protein extraction Features of Halt Phosphatase Inhibitor Cocktail • Devoid of protease inhibitors To protect against both proteases and phosphatases use Halt Protease and Phosphatase Inhibitor Cocktail • Dialyzable Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components • Supplied as a 100X concentrate effective when used at a final concentration of 1X however samples that contain particularly high levels of phosphatase activity may require a final concentration of 2 3X • Compatibility compatible with most detergent based cell lysis reagents including all Thermo Scientific Pierce Cell Lysis Solutions • All in one format inhibits both serine threonine phosphatases and protein tyrosine phosphatases • Compatiblewith popular protein assays works with standard protein assays including BCA and coomassie Bradford assays • Convenient storage refrigerator 2 to 8°C storage saves valuable space in the freezer and provides for immediate use • Multiple sizes select a package size of the 100X cocktail that is ideal for any particular scale and workflow of protein extraction experiment Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine threonine phosphatases as well as protein tyrosine phosphatases PTPs during cell lysis The cocktail contains a mixture of four inhibitors of broad specificity including sodium fluoride sodium orthovanadate sodium pyrophosphate and beta glycerophosphate Conveniently packaged in a 100X format the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays Phosphorylation and dephosphorylation comprise a molecular on off switch that regulates many key biological pathways within the cell including signal transduction cell division and apoptosis To effectively study phosphorylation activation states one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis Halt Phosphatase Inhibitor Cocktail accomplishes this task Related Products Pierce Phosphatase Inhibitor Mini Tablets
    https://www.bioz.com/result/phosphatase inhibitor mixture/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    phosphatase inhibitor mixture - by Bioz Stars, 2020-11
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis
    Article Snippet: .. Protein isolation and western blotting analysis At the end of 14 days of culture, cells were released from scaffolds with 1X Trypsin followed by pelleting cells at 1000×g and finally lysing the pellet with Pierce IP lysis buffer supplemented with 1X Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, Rockford, IL, USA). .. After centrifugation of the lysate at 15,000×g for 10 min at 4°C, supernatants were collected and the protein concentration was measured by the bicinchoninic acid (BCA) method.

    Western Blot:

    Article Title: Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis
    Article Snippet: .. Protein isolation and western blotting analysis At the end of 14 days of culture, cells were released from scaffolds with 1X Trypsin followed by pelleting cells at 1000×g and finally lysing the pellet with Pierce IP lysis buffer supplemented with 1X Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, Rockford, IL, USA). .. After centrifugation of the lysate at 15,000×g for 10 min at 4°C, supernatants were collected and the protein concentration was measured by the bicinchoninic acid (BCA) method.

    Article Title: Impaired myogenic development, differentiation and function in hESC-derived SMA myoblasts and myotubes
    Article Snippet: .. For capillary western blotting, cells were lysed for 5 min using 1x RIPA buffer (#R0278, Sigma-Aldrich) with Phosphatase Inhibitor Cocktail (#04906845001, Roche Applied Science) and complete Protease Inhibitor Cocktail (#04693124001, Roche Applied Science) or RIPA supplemented with Halt Protease inhibitor cocktail (#20–188, Millipore) and Halt Phosphatase inhibitor cocktail (#1861277, ThermoFisher Scientific). .. Total protein in the supernatant was quantified by bicinchoninic acid assay (#23225, ThermoFisher Scientific) or Nanodrop 1000.

    Protease Inhibitor:

    Article Title: Impaired myogenic development, differentiation and function in hESC-derived SMA myoblasts and myotubes
    Article Snippet: .. For capillary western blotting, cells were lysed for 5 min using 1x RIPA buffer (#R0278, Sigma-Aldrich) with Phosphatase Inhibitor Cocktail (#04906845001, Roche Applied Science) and complete Protease Inhibitor Cocktail (#04693124001, Roche Applied Science) or RIPA supplemented with Halt Protease inhibitor cocktail (#20–188, Millipore) and Halt Phosphatase inhibitor cocktail (#1861277, ThermoFisher Scientific). .. Total protein in the supernatant was quantified by bicinchoninic acid assay (#23225, ThermoFisher Scientific) or Nanodrop 1000.

    Purification:

    Article Title: Ezh2 does not mediate retinal ganglion cell homeostasis or their susceptibility to injury
    Article Snippet: .. Briefly, RGCs purified from new born (P0) mouse pups were lysed by sonication in ice-cold RIPA buffer (Cat. No. 20–188; Milipore, Billerica, MA) containing proteinase cocktail inhibitor (Ref: 05892953001; Roche, Indianapolis, IN) and phosphatase inhibitor cocktail (Cat. No. 78420; Thermo Scientific, Waltham, MA). .. Protein concentration was measured using a NanoDrop 2000 Spectrophotometer (Thermo Scientific).

    Sonication:

    Article Title: Hypoxia-induced 26S proteasome dysfunction increases immunogenicity of mesenchymal stem cells
    Article Snippet: .. Briefly, the cell lysates were prepared by sonication in 20 mM Bis-tris, 500 mM ε-aminocaproic acid 20 mM NaCl, 2 mM EDTA (pH 8.0), and Glycerol 10% supplemented with 1 × Halt protease and phosphatase inhibitor cocktail (Thermo Scientific), and 1.5% n -Dodecyl β- d -maltoside (Sigma). ..

    Article Title: Ezh2 does not mediate retinal ganglion cell homeostasis or their susceptibility to injury
    Article Snippet: .. Briefly, RGCs purified from new born (P0) mouse pups were lysed by sonication in ice-cold RIPA buffer (Cat. No. 20–188; Milipore, Billerica, MA) containing proteinase cocktail inhibitor (Ref: 05892953001; Roche, Indianapolis, IN) and phosphatase inhibitor cocktail (Cat. No. 78420; Thermo Scientific, Waltham, MA). .. Protein concentration was measured using a NanoDrop 2000 Spectrophotometer (Thermo Scientific).

    Lysis:

    Article Title: Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis
    Article Snippet: .. Protein isolation and western blotting analysis At the end of 14 days of culture, cells were released from scaffolds with 1X Trypsin followed by pelleting cells at 1000×g and finally lysing the pellet with Pierce IP lysis buffer supplemented with 1X Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, Rockford, IL, USA). .. After centrifugation of the lysate at 15,000×g for 10 min at 4°C, supernatants were collected and the protein concentration was measured by the bicinchoninic acid (BCA) method.

    Article Title: March1-dependent modulation of donor MHC II on CD103+ dendritic cells mitigates alloimmunity
    Article Snippet: .. Cells were then washed with ice-cold Dulbecco’s PBS and lysed in NP-40 lysis buffer (containing 1% NP-40, 150 mM NaCl, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM phenylmethylsulfonyl fluoride (PMSF), 1× HALT protease and phosphatase inhibitor cocktail (Thermo Scientific)). .. For immunoprecipitation (IP), 1 mg of cell extract was incubated with 5 µg of anti-I-A/I-E (MHC II—clone M5/114, eBioscience) for 2 h at 4 °C followed by incubation with 20 µL of protein A (50% slurry, GE Healthcare) plus sepharose beads for either 2 h at room temperature or overnight at 4 °C.

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  • 99
    Thermo Fisher phosphatase inhibitor mixture
    Phosphatase Inhibitor Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatase inhibitor mixture/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    phosphatase inhibitor mixture - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

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