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Merck & Co phosphatase inhibitor cocktail
Phosphatase Inhibitor Cocktail, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphatase inhibitor cocktail/product/Merck & Co
Average 93 stars, based on 21 article reviews
Price from $9.99 to $1999.99
phosphatase inhibitor cocktail - by Bioz Stars, 2020-08
93/100 stars

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Transfection:

Article Title: CtIP tetramer assembly is required for DNA-end resection and repair
Article Snippet: .. 48 h after transfection, cells were scraped off 15 cm plates, pelleted and resuspended in 0.5 ml of lysis buffer (20 mM Tris-HCl pH 7.5, 40 mM NaCl, 2 mM MgCl2 , 10% glycerol, 0.5% NP-40, EDTA-free protease inhibitors (Roche), 10 mM N-ethylmaleimide (Sigma-Aldrich), phosphatase inhibitor cocktail (42 μg/ml NaF, 0.22 mg/ml beta-glycerophosphate, 37 μg/ml Na3 VO4 , 2.2 mg/ml sodium pyrophosphate) containing 100 U of benzonase (Merck). .. After a 5 min incubation at room temperature, 50 μl of 5 M NaCl were added to the mix and tubes were left on ice for 15 min. Extracts were pelleted at 15 000 rpm at 4 °C for 45 min. Supernatants were recovered and diluted with 0.5 ml of lysis buffer with no benzonase.

Radio Immunoprecipitation:

Article Title: Two Domains of the V Protein of Virulent Canine Distemper Virus Selectively Inhibit STAT1 and STAT2 Nuclear Import ▿
Article Snippet: .. At 2 days postinfection or posttransfection, the cells were washed with cold PBS and lysed at 4°C in radioimmunoprecipitation assay (RIPA) buffer (150 mM NaCl [500 mM NaCl to extract the C protein], 1% NP-40, 0.5% Na-deoxycholate, 0.1% sodium dodecyl sulfate]SDS], 50 mM Tris-Cl, pH 7.4) containing Halt Protease Inhibitor Cocktail (Socochim) and Phosphatase Inhibitor Cocktail Set II (Merck) to protect phosphorylated STAT proteins. .. After centrifugation, Laemmli sample buffer (Bio-Rad) supplemented with dl -dithiothreitol (Fluka) was added to the samples, and they were boiled for 5 min and separated by SDS-polyacrylamide gel electrophoresis.

Protease Inhibitor:

Article Title: Two Domains of the V Protein of Virulent Canine Distemper Virus Selectively Inhibit STAT1 and STAT2 Nuclear Import ▿
Article Snippet: .. At 2 days postinfection or posttransfection, the cells were washed with cold PBS and lysed at 4°C in radioimmunoprecipitation assay (RIPA) buffer (150 mM NaCl [500 mM NaCl to extract the C protein], 1% NP-40, 0.5% Na-deoxycholate, 0.1% sodium dodecyl sulfate]SDS], 50 mM Tris-Cl, pH 7.4) containing Halt Protease Inhibitor Cocktail (Socochim) and Phosphatase Inhibitor Cocktail Set II (Merck) to protect phosphorylated STAT proteins. .. After centrifugation, Laemmli sample buffer (Bio-Rad) supplemented with dl -dithiothreitol (Fluka) was added to the samples, and they were boiled for 5 min and separated by SDS-polyacrylamide gel electrophoresis.

Article Title: The Neuropeptides Vasoactive Intestinal Peptide and Pituitary Adenylate Cyclase-Activating Polypeptide Control HIV-1 Infection in Macrophages Through Activation of Protein Kinases A and C
Article Snippet: .. Immunoblotting for Detection of PKA and PKC Activation Macrophages (1.5 × 106 /4 mL/flask, 25 cm2 flasks) were treated with VIP or PACAP for different time-points and then proteins were extracted using RIPA buffer (Thermo Fischer) with Protease Inhibitor Cocktail Set III and Phosphatase Inhibitor Cocktail Set II (Merck). .. The protein concentration was quantified by Qubit 2.0 Protein Assay Kit (Thermo Fisher).

Article Title: Formaldehyde Crosses the Human Placenta and Affects Human Trophoblast Differentiation and Hormonal Functions
Article Snippet: .. Protein preparation and immunoblot analysis Total cell lysates were prepared in lysis buffer (Life Technologies; 50 mM Tris pH 7.4, 250 mM NaCl, 50 mM NaF, 5 mM EDTA, 1% Nonidet P40, 0.02% NaN3, 1 mM sodium orthovanadate) supplemented with a protease inhibitor cocktail (Merck) and a phosphatase inhibitor cocktail (Merck). .. Protein samples were resolved by SDS-PAGE and blotted onto nitrocellulose membranes.

Article Title: Phosphoproteome-based kinase activity profiling reveals the critical role of MAP2K2 and PLK1 in neuronal autophagy
Article Snippet: .. Cell pellets were lysed with lysis buffer (Thermo, 89900) supplemented with Phosphatase Inhibitor Cocktail (Merck, 524625) and Protease Inhibitor Cocktail (Merck, 539137) on ice using a high-intensity ultrasonic processor (Scientz) for 30 min. .. The protein concentration was determined with a 2-D Quant kit according to the manufacturer's instructions.

Article Title:
Article Snippet: .. After incubation, the cells were washed twice with cold PBS containing 0.01 volume of Phosphatase Inhibitor Cocktail and then lysed in Phosphosafe Extraction Buffer (Merck, Rahway, NJ; 100 μl) containing Protease Inhibitor Cocktail (4 μl), Phosphatase Inhibitor Cocktail (2 μl), and other protease inhibitors (25 μg/ml N -acetyl-leucinal-leucinal-norleucinal [ALLN], 1 μg/ml pepstatin A, 2 μg/ml aprotinin, 10 μg/ml leupeptin, 200 μM phenylmethylsulfonyl fluoride). ..

Activation Assay:

Article Title: The Neuropeptides Vasoactive Intestinal Peptide and Pituitary Adenylate Cyclase-Activating Polypeptide Control HIV-1 Infection in Macrophages Through Activation of Protein Kinases A and C
Article Snippet: .. Immunoblotting for Detection of PKA and PKC Activation Macrophages (1.5 × 106 /4 mL/flask, 25 cm2 flasks) were treated with VIP or PACAP for different time-points and then proteins were extracted using RIPA buffer (Thermo Fischer) with Protease Inhibitor Cocktail Set III and Phosphatase Inhibitor Cocktail Set II (Merck). .. The protein concentration was quantified by Qubit 2.0 Protein Assay Kit (Thermo Fisher).

Incubation:

Article Title: Phosphorylation of Chromosome Core Components May Serve as Axis Marks for the Status of Chromosomal Events during Mammalian Meiosis
Article Snippet: .. For phosphatase treatment, nuclear extracts were incubated with λ-phosphatase (New England Biolabs) in the presence or absence of phosphatase inhibitor cocktail (Merck) for 90 min at 30°C. .. For fractionation of nuclear extracts, testes were homogenized in a buffer containing 0.32 M Sucrose, 10 mM HEPES pH 7.4, 1 mM phenylmethylsulfonyl fluoride (PMSF) and the complete protease inhibitor cocktail (Roche).

Article Title:
Article Snippet: .. After incubation, the cells were washed twice with cold PBS containing 0.01 volume of Phosphatase Inhibitor Cocktail and then lysed in Phosphosafe Extraction Buffer (Merck, Rahway, NJ; 100 μl) containing Protease Inhibitor Cocktail (4 μl), Phosphatase Inhibitor Cocktail (2 μl), and other protease inhibitors (25 μg/ml N -acetyl-leucinal-leucinal-norleucinal [ALLN], 1 μg/ml pepstatin A, 2 μg/ml aprotinin, 10 μg/ml leupeptin, 200 μM phenylmethylsulfonyl fluoride). ..

Lysis:

Article Title: CtIP tetramer assembly is required for DNA-end resection and repair
Article Snippet: .. 48 h after transfection, cells were scraped off 15 cm plates, pelleted and resuspended in 0.5 ml of lysis buffer (20 mM Tris-HCl pH 7.5, 40 mM NaCl, 2 mM MgCl2 , 10% glycerol, 0.5% NP-40, EDTA-free protease inhibitors (Roche), 10 mM N-ethylmaleimide (Sigma-Aldrich), phosphatase inhibitor cocktail (42 μg/ml NaF, 0.22 mg/ml beta-glycerophosphate, 37 μg/ml Na3 VO4 , 2.2 mg/ml sodium pyrophosphate) containing 100 U of benzonase (Merck). .. After a 5 min incubation at room temperature, 50 μl of 5 M NaCl were added to the mix and tubes were left on ice for 15 min. Extracts were pelleted at 15 000 rpm at 4 °C for 45 min. Supernatants were recovered and diluted with 0.5 ml of lysis buffer with no benzonase.

Article Title: The mammalian tRNA ligase complex mediates splicing of XBP1 mRNA and controls antibody secretion in plasma cells
Article Snippet: .. Preparation of whole-cell extracts HeLa cells were grown to confluency and harvested in 1× lysis buffer (30 mM HEPES pH 7.4, 100 mM KCl, 5 mM MgCl2 , 10% (v/v) glycerol, 0.2% (v/v) Nonidet P-40, 0.5 mM DTT, 0.1 mM phenylmethylsulfonyl fluoride (PMSF)) supplemented with Phosphatase Inhibitor Cocktail Set II (Merck). ..

Article Title: Formaldehyde Crosses the Human Placenta and Affects Human Trophoblast Differentiation and Hormonal Functions
Article Snippet: .. Protein preparation and immunoblot analysis Total cell lysates were prepared in lysis buffer (Life Technologies; 50 mM Tris pH 7.4, 250 mM NaCl, 50 mM NaF, 5 mM EDTA, 1% Nonidet P40, 0.02% NaN3, 1 mM sodium orthovanadate) supplemented with a protease inhibitor cocktail (Merck) and a phosphatase inhibitor cocktail (Merck). .. Protein samples were resolved by SDS-PAGE and blotted onto nitrocellulose membranes.

Article Title: Phosphoproteome-based kinase activity profiling reveals the critical role of MAP2K2 and PLK1 in neuronal autophagy
Article Snippet: .. Cell pellets were lysed with lysis buffer (Thermo, 89900) supplemented with Phosphatase Inhibitor Cocktail (Merck, 524625) and Protease Inhibitor Cocktail (Merck, 539137) on ice using a high-intensity ultrasonic processor (Scientz) for 30 min. .. The protein concentration was determined with a 2-D Quant kit according to the manufacturer's instructions.

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    Merck & Co phosphatase inhibitor cocktail set ii
    Phosphatase Inhibitor Cocktail Set Ii, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatase inhibitor cocktail set ii/product/Merck & Co
    Average 90 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    phosphatase inhibitor cocktail set ii - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    93
    Merck & Co phosstop phosphatase inhibitor cocktails
    Phosstop Phosphatase Inhibitor Cocktails, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosstop phosphatase inhibitor cocktails/product/Merck & Co
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    phosstop phosphatase inhibitor cocktails - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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