phix174 rfi plasmid  (New England Biolabs)


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  • 94
    Name:
    PhiX174 RF I DNA
    Description:
    PhiX174 RF I DNA 150 ug
    Catalog Number:
    n3021l
    Price:
    300
    Size:
    150 ug
    Category:
    Genomic DNA
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    Structured Review

    New England Biolabs phix174 rfi plasmid
    PhiX174 RF I DNA
    PhiX174 RF I DNA 150 ug
    https://www.bioz.com/result/phix174 rfi plasmid/product/New England Biolabs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    phix174 rfi plasmid - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: DNA Binding in High Salt: Analysing the Salt Dependence of Replication Protein A3 from the Halophile Haloferax volcanii
    Article Snippet: .. Agarose Gel Retardation 500 ng of PhiX174 ssDNA (New England Biolabs) was incubated with the indicated amounts of HvRPA3 in 20 mM Tris, 15 mM MgCl2 , 2 mM DTT, 50 μ g/mL BSA, 6% glycerol, and 1 M KCl for 10 minutes at 37°C prior to loading on a 0.6% agarose gel. .. Following electrophoresis in 1x TBE buffer, DNA was visualised via ethidium bromide staining under UV illumination.

    Labeling:

    Article Title: DNA looping by two-site restriction endonucleases: heterogeneous probability distributions for loop size and unbinding force
    Article Snippet: .. PhiX174 DNA was purchased from NEB and was labeled by digesting with XhoI and end-labeling with dATP-14-biotin (Invitrogen). .. The DNA was then digested with StuI, purified using the Promega Wizard DNA clean up kit, and end-labeled with dUTP-11-DIG.

    Purification:

    Article Title: In vitro role of Rad54 in Rad51-ssDNA filament-dependent homology search and synaptic complexes formation
    Article Snippet: .. Rad51 at a final concentration of 12 μM (one protein per 3 nt) was introduced into the reaction and incubated 10 min followed by RPA at a final concentration of 1.44 μM (one protein per 25 nt) also during 10 min. Rad54 (wild-type Rad54 or Rad54K341R as stated in the results) and dsDNA (homologous or heterologous, as stated in the results) were added together in a final concentration of 175 nM and 25 nM in molecules of dsDNA (seven proteins per dsDNA molecule) in a final volume of 14 μL during 20 min. For the homologous donor, pUC19 plasmid was used, while PhiX174 RFI was used as heterologous DNA, both purchased from New England Biolabs and purified on MiniQ ion exchange chromatography column. ..

    Concentration Assay:

    Article Title: In vitro role of Rad54 in Rad51-ssDNA filament-dependent homology search and synaptic complexes formation
    Article Snippet: .. Rad51 at a final concentration of 12 μM (one protein per 3 nt) was introduced into the reaction and incubated 10 min followed by RPA at a final concentration of 1.44 μM (one protein per 25 nt) also during 10 min. Rad54 (wild-type Rad54 or Rad54K341R as stated in the results) and dsDNA (homologous or heterologous, as stated in the results) were added together in a final concentration of 175 nM and 25 nM in molecules of dsDNA (seven proteins per dsDNA molecule) in a final volume of 14 μL during 20 min. For the homologous donor, pUC19 plasmid was used, while PhiX174 RFI was used as heterologous DNA, both purchased from New England Biolabs and purified on MiniQ ion exchange chromatography column. ..

    Incubation:

    Article Title: DNA Binding in High Salt: Analysing the Salt Dependence of Replication Protein A3 from the Halophile Haloferax volcanii
    Article Snippet: .. Agarose Gel Retardation 500 ng of PhiX174 ssDNA (New England Biolabs) was incubated with the indicated amounts of HvRPA3 in 20 mM Tris, 15 mM MgCl2 , 2 mM DTT, 50 μ g/mL BSA, 6% glycerol, and 1 M KCl for 10 minutes at 37°C prior to loading on a 0.6% agarose gel. .. Following electrophoresis in 1x TBE buffer, DNA was visualised via ethidium bromide staining under UV illumination.

    Article Title: DNA Binding in High Salt: Analysing the Salt Dependence of Replication Protein A3 from the Halophile Haloferax volcanii
    Article Snippet: .. However, with optimisation, band shifts were observed with agarose gels run in 1× TBE buffer, following incubation of increasing quantities of HvRPA3 with PhiX174 ssDNA in 1 M KCl ( ). ..

    Article Title: In vitro role of Rad54 in Rad51-ssDNA filament-dependent homology search and synaptic complexes formation
    Article Snippet: .. Rad51 at a final concentration of 12 μM (one protein per 3 nt) was introduced into the reaction and incubated 10 min followed by RPA at a final concentration of 1.44 μM (one protein per 25 nt) also during 10 min. Rad54 (wild-type Rad54 or Rad54K341R as stated in the results) and dsDNA (homologous or heterologous, as stated in the results) were added together in a final concentration of 175 nM and 25 nM in molecules of dsDNA (seven proteins per dsDNA molecule) in a final volume of 14 μL during 20 min. For the homologous donor, pUC19 plasmid was used, while PhiX174 RFI was used as heterologous DNA, both purchased from New England Biolabs and purified on MiniQ ion exchange chromatography column. ..

    End Labeling:

    Article Title: DNA looping by two-site restriction endonucleases: heterogeneous probability distributions for loop size and unbinding force
    Article Snippet: .. PhiX174 DNA was purchased from NEB and was labeled by digesting with XhoI and end-labeling with dATP-14-biotin (Invitrogen). .. The DNA was then digested with StuI, purified using the Promega Wizard DNA clean up kit, and end-labeled with dUTP-11-DIG.

    Ion Exchange Chromatography:

    Article Title: In vitro role of Rad54 in Rad51-ssDNA filament-dependent homology search and synaptic complexes formation
    Article Snippet: .. Rad51 at a final concentration of 12 μM (one protein per 3 nt) was introduced into the reaction and incubated 10 min followed by RPA at a final concentration of 1.44 μM (one protein per 25 nt) also during 10 min. Rad54 (wild-type Rad54 or Rad54K341R as stated in the results) and dsDNA (homologous or heterologous, as stated in the results) were added together in a final concentration of 175 nM and 25 nM in molecules of dsDNA (seven proteins per dsDNA molecule) in a final volume of 14 μL during 20 min. For the homologous donor, pUC19 plasmid was used, while PhiX174 RFI was used as heterologous DNA, both purchased from New England Biolabs and purified on MiniQ ion exchange chromatography column. ..

    Recombinase Polymerase Amplification:

    Article Title: In vitro role of Rad54 in Rad51-ssDNA filament-dependent homology search and synaptic complexes formation
    Article Snippet: .. Rad51 at a final concentration of 12 μM (one protein per 3 nt) was introduced into the reaction and incubated 10 min followed by RPA at a final concentration of 1.44 μM (one protein per 25 nt) also during 10 min. Rad54 (wild-type Rad54 or Rad54K341R as stated in the results) and dsDNA (homologous or heterologous, as stated in the results) were added together in a final concentration of 175 nM and 25 nM in molecules of dsDNA (seven proteins per dsDNA molecule) in a final volume of 14 μL during 20 min. For the homologous donor, pUC19 plasmid was used, while PhiX174 RFI was used as heterologous DNA, both purchased from New England Biolabs and purified on MiniQ ion exchange chromatography column. ..

    Plasmid Preparation:

    Article Title: In vitro role of Rad54 in Rad51-ssDNA filament-dependent homology search and synaptic complexes formation
    Article Snippet: .. Rad51 at a final concentration of 12 μM (one protein per 3 nt) was introduced into the reaction and incubated 10 min followed by RPA at a final concentration of 1.44 μM (one protein per 25 nt) also during 10 min. Rad54 (wild-type Rad54 or Rad54K341R as stated in the results) and dsDNA (homologous or heterologous, as stated in the results) were added together in a final concentration of 175 nM and 25 nM in molecules of dsDNA (seven proteins per dsDNA molecule) in a final volume of 14 μL during 20 min. For the homologous donor, pUC19 plasmid was used, while PhiX174 RFI was used as heterologous DNA, both purchased from New England Biolabs and purified on MiniQ ion exchange chromatography column. ..

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  • 99
    New England Biolabs phix174 rfi
    Phix174 Rfi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phix174 rfi/product/New England Biolabs
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    phix174 rfi - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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