pher2 y1248  (Danaher Inc)

Journal: International Journal of Molecular Sciences

Article Title: Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer

doi: 10.3390/ijms25031374

Figure Lengend Snippet: Depletion of LDOC1 promotes activation of EGFR, HER2, and AXL in EGFR M NSCLC cells. ( a , b ) LDOC1 depletion upregulated expression of phosphorylated (p) and total (t) EGFR and its related RTKs. PC9 ( a ) and HCC827 ( b ) cells were serum-starved for 16 h before being treated with EGF (10 nM) for 30 min at 37 °C. Whole-cell lysates were subjected to Western blot analysis using the indicated antibodies. GAPDH was used as a loading control. The summary of the semiquantitative data is shown as a plot chart in . ( c ) LDOC1 depletion sustained prolonged activation of EGFR, HER2, and AXL. PC9 cells were serum-starved overnight and then treated with EGF (10 nM) at 37 °C for 10 min, washed with PBS, and then incubated in normal growth medium at 37 °C. Whole-cell lysates were prepared at the indicated time and analyzed as described in ( a , b ). The value of pRTK/GAPDH at time 0 was set as 1 and fold changes > 1 were shown in red. ( d ) Semiquantitative evaluation of ( c ). Densitometry was performed on blots with different exposures and average results were plotted using arbitrary unit (a.u.) for pEGFR, pHER2, pHER3, and pAXL.

Article Snippet: The antibodies used in the present study were anti-EGFR (GTX121919), anti-pEGFR Y1068 (GTX132810), anti-HER2 (GTX100509), HER3 (GTX100256), anti-GAPDH (GTX100118), anti-AP1M1 (GTX64907), and anti-AP2M1 (GTX#113332), which were purchased from GeneTex; anti-AXL (C89E7), which was purchased from Cell Signaling Technology; anti-pHER2 Y1248 (AF1768-SP) and anti-pHER3 Y1262 (AF5817-SP), which were purchased from R&D system; and anti-EGFR (51071-2-AP, Proteintech, Rosemont, IL, USA), which was purchased from ProteinTech.

Techniques: Activation Assay, Expressing, Western Blot, Control, Incubation

Journal: Clinical Proteomics

Article Title: Quantitative proteomic analysis of HER2 protein expression in PDAC tumors

doi: 10.1186/s12014-024-09476-7

Figure Lengend Snippet: RPPA quantification of HER2 Total , pHER2 Y1248 , pHER3 Y1289 and correlation with CLIA-approved clinical IHC scoring. Fisher's Exact tests were performed using SAS software (v9.4) to compare the RPPA abundances of HER2 Total , pHER2 Y1248 , and pHER3 Y1289 between groups. An asterisk (*) indicates p < 0.05. NS not significant

Article Snippet: Fisher's Exact tests were performed using SAS software (v9.4) to compare the RPPA abundances of HER2 Total , pHER2 Y1248 , and pHER3 Y1289 between groups.

Techniques: Software

Journal: International Journal of Molecular Sciences

Article Title: Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer

doi: 10.3390/ijms25031374

Figure Lengend Snippet: Depletion of LDOC1 promotes activation of EGFR, HER2, and AXL in EGFR M NSCLC cells. ( a , b ) LDOC1 depletion upregulated expression of phosphorylated (p) and total (t) EGFR and its related RTKs. PC9 ( a ) and HCC827 ( b ) cells were serum-starved for 16 h before being treated with EGF (10 nM) for 30 min at 37 °C. Whole-cell lysates were subjected to Western blot analysis using the indicated antibodies. GAPDH was used as a loading control. The summary of the semiquantitative data is shown as a plot chart in . ( c ) LDOC1 depletion sustained prolonged activation of EGFR, HER2, and AXL. PC9 cells were serum-starved overnight and then treated with EGF (10 nM) at 37 °C for 10 min, washed with PBS, and then incubated in normal growth medium at 37 °C. Whole-cell lysates were prepared at the indicated time and analyzed as described in ( a , b ). The value of pRTK/GAPDH at time 0 was set as 1 and fold changes > 1 were shown in red. ( d ) Semiquantitative evaluation of ( c ). Densitometry was performed on blots with different exposures and average results were plotted using arbitrary unit (a.u.) for pEGFR, pHER2, pHER3, and pAXL.

Article Snippet: The antibodies used in the present study were anti-EGFR (GTX121919), anti-pEGFR Y1068 (GTX132810), anti-HER2 (GTX100509), HER3 (GTX100256), anti-GAPDH (GTX100118), anti-AP1M1 (GTX64907), and anti-AP2M1 (GTX#113332), which were purchased from GeneTex; anti-AXL (C89E7), which was purchased from Cell Signaling Technology; anti-pHER2 Y1248 (AF1768-SP) and anti-pHER3 Y1262 (AF5817-SP), which were purchased from R&D system; and anti-EGFR (51071-2-AP, Proteintech, Rosemont, IL, USA), which was purchased from ProteinTech.

Techniques: Activation Assay, Expressing, Western Blot, Control, Incubation