phenylmethylsulfonylfluoride  (Millipore)

 
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    Name:
    Phenylmethanesulfonyl fluoride
    Description:

    Catalog Number:
    P7626
    Price:
    None
    Applications:
    Phenylmethanesulfonyl fluoride has been used in following applications:. cell fractionation.. used as a supplement in nuclear protein extraction.. inhibitor of cholesterol esterase (CE) and pseudocholinesterase (PCE).. used for the collection of blood prior to centrifugation to quantify plasma ANP levels.
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    Structured Review

    Millipore phenylmethylsulfonylfluoride
    Phenylmethanesulfonyl fluoride

    https://www.bioz.com/result/phenylmethylsulfonylfluoride/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethylsulfonylfluoride - by Bioz Stars, 2021-04
    86/100 stars

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    Related Articles

    other:

    Article Title: Interrogating Parkinson's disease LRRK2 kinase pathway activity by assessing Rab10 phosphorylation in human neutrophils
    Article Snippet: The PF-06447475 inhibitor was purchased from R & D systems (#5716), diisopropylfluorophosphate (DIFP) was from Sigma (Cat# D0879) and phenylmethane sulfonyl fluoride (PMSF) was from Sigma (Cat# 78830).

    Article Title: Utilization of Seafood Processing By-Products for Production of Proteases by Paenibacillus sp. TKU052 and Their Application in Biopeptides’ Preparation
    Article Snippet: Materials Azocasein, DPPH, lung acetone powder from rabbit, 2-ME, tyrosine, phenylmethylsulfonyl fluoride, and N -Hippuryl-His-Leu (HHL) were bought from Sigma Co. (St. Louis, MO, USA).

    Article Title: Matrix metalloproteinase-2-induced epidermal growth factor receptor transactivation impairs redox balance in vascular smooth muscle cells and facilitates vascular contraction
    Article Snippet: Tyrphostin AG 1478, Phenylephrine, Apocynin, Peg-Catalase (PG-Cat), Dihydroethidium (DHE), phenanthroline, Phenylmethylsulfonyl fluoride were purchased from Sigma Chemical Co. (St. Louis, MO, USA).

    Nucleic Acid Electrophoresis:

    Article Title: Intracellular Topology and Epitope Shielding of Poliovirus 3A Protein
    Article Snippet: Both tubes were placed on a rotator at 4°C for 10 min; 100-μl aliquots were incubated with various concentrations of proteinase K (Sigma) for 15 min at 37°C. .. Proteolysis was stopped by the addition of 20 μl of a solution containing 1 mM phenylmethylsulfonyl fluoride (Sigma) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer. ..

    SDS Page:

    Article Title: Intracellular Topology and Epitope Shielding of Poliovirus 3A Protein
    Article Snippet: Both tubes were placed on a rotator at 4°C for 10 min; 100-μl aliquots were incubated with various concentrations of proteinase K (Sigma) for 15 min at 37°C. .. Proteolysis was stopped by the addition of 20 μl of a solution containing 1 mM phenylmethylsulfonyl fluoride (Sigma) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer. ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Kavain Inhibition of LPS-Induced TNF-α via ERK/LITAF
    Article Snippet: The following antibodies were directed against p-ERK2 (sc-16981): LITAF (sc-66945), GAPDH (sc-365062), actin (sc-1615), all from Santa Cruz Biotechnology, or tubulin (T6199, Sigma). .. Kavain (Cat500-64-1, AvaChem Scientific), E-64 (inhibition of cysteine protease, Cat# E3132, Sigma), PMSF (inhibition of serine protease, Cat# P7626, Sigma), and MNS (inhibition of cysteine protease and tyrosine kinase, Cat# S4921, Selleckchem) were dissolved in 1% DMSO just before use. .. WT mice (LITAF+/+ ) and ERK2-mutant mice (ERK2–/– ) were injected intraperitoneally with 1.

    Inhibition:

    Article Title: Kavain Inhibition of LPS-Induced TNF-α via ERK/LITAF
    Article Snippet: The following antibodies were directed against p-ERK2 (sc-16981): LITAF (sc-66945), GAPDH (sc-365062), actin (sc-1615), all from Santa Cruz Biotechnology, or tubulin (T6199, Sigma). .. Kavain (Cat500-64-1, AvaChem Scientific), E-64 (inhibition of cysteine protease, Cat# E3132, Sigma), PMSF (inhibition of serine protease, Cat# P7626, Sigma), and MNS (inhibition of cysteine protease and tyrosine kinase, Cat# S4921, Selleckchem) were dissolved in 1% DMSO just before use. .. WT mice (LITAF+/+ ) and ERK2-mutant mice (ERK2–/– ) were injected intraperitoneally with 1.

    Centrifugation:

    Article Title: Latent myostatin has significant activity and this activity is controlled more efficiently by WFIKKN1 than by WFIKKN2
    Article Snippet: .. Protein precipitates were removed by centrifugation at 3500 g for 15 min and the protein solution was dialyzed against 3 × 3 L of 100 mm Tris/HCl, 100 μm phenylmethanesulfonyl fluoride and 0.005% 2-mercaptoethanol (pH 8.0) at 4 °C for 36 h. Precipitates were removed by centrifugation at 3500 g for 15 min and the protein solution was then concentrated on an Amicon stirred ultrafiltration cell (EMD Millipore, Billerica, MA USA) and applied to a 20-mL Strep-Tactin Sepharose column. ..

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  • 97
    Millipore phenylmethylsulfonylfluoride
    Phenylmethylsulfonylfluoride, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylmethylsulfonylfluoride/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethylsulfonylfluoride - by Bioz Stars, 2021-04
    97/100 stars
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