phenylmethylsulfonylfluoride pmsf  (Millipore)

 
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    Name:
    PMSF
    Description:

    Catalog Number:
    pmsf-ro
    Price:
    None
    Applications:
    PMSF (C7H7O2SF) inhibits serine proteases such as chymotrypsin, trypsin, thrombin, and the cysteine protease papain (reversible by DTT treatment). It does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.
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    Structured Review

    Millipore phenylmethylsulfonylfluoride pmsf
    PMSF

    https://www.bioz.com/result/phenylmethylsulfonylfluoride pmsf/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethylsulfonylfluoride pmsf - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Bacillus sp. JR3 esterase LipJ: A new mesophilic enzyme showing traces of a thermophilic past
    Article Snippet: Residual activity was measured at 30°C and pH 7 using a conventional assay on p NP-butyrate, and expressed as a percentage of activity without ions or inhibitors, respectively. .. Supernatant samples of Bacillus sp. JR3 were also incubated in the presence of 1 mM PMSF (phenylmethylsulfonyl fluoride, Sigma) to measure the effect of this serine inhibitor on enzyme activity. .. Kinetic parameters (Vmax and Km) were determined under optimal assay conditions by fitting hyperbolic Michaelis-Menten curves with GraphPad Prism ® software version 6.

    Article Title: A Novel Pathway for Mammary Epithelial Cell Invasion Induced by the Helix-Loop-Helix Protein Id-1
    Article Snippet: Briefly, gels consisted of 8 to 10% polyacrylamide and 3 mg of α-casein or gelatin (Sigma)/ml. .. Concentrated conditioned medium was mixed with nonreducing Laemmli sample buffer and incubated at 37°C for 15 min. After electrophoresis, the gels were incubated for 1 h in 2.5% Triton X-100 at room temperature, followed by 24 to 48 h in substrate buffer (100 mM Tris-HCl [pH 7.4]–15 mM CaCl2 ) in the absence or presence of GM6001 (0.2 mM in DMSO; supplied by Glycomed Corporation and obtained from Z. Werb [ ]), EDTA (10 mM), ortho -phenanthroline (1 mM in DMSO; Sigma), PMSF (5 mM), or AEBSF (0.5 mM; Calbiochem). .. The gels were stained with Coomassie blue for 30 min and were destained with 30% methanol–10% acetic acid.

    Activity Assay:

    Article Title: Bacillus sp. JR3 esterase LipJ: A new mesophilic enzyme showing traces of a thermophilic past
    Article Snippet: Residual activity was measured at 30°C and pH 7 using a conventional assay on p NP-butyrate, and expressed as a percentage of activity without ions or inhibitors, respectively. .. Supernatant samples of Bacillus sp. JR3 were also incubated in the presence of 1 mM PMSF (phenylmethylsulfonyl fluoride, Sigma) to measure the effect of this serine inhibitor on enzyme activity. .. Kinetic parameters (Vmax and Km) were determined under optimal assay conditions by fitting hyperbolic Michaelis-Menten curves with GraphPad Prism ® software version 6.

    Article Title: Ku86 exists as both a full-length and a protease-sensitive natural variant in multiple myeloma cells
    Article Snippet: The reaction mix was incubated at 37°C; and at various time points, aliquots were removed and resolved in a 12.5% SDS-PAGE gel for carbohydrate (CHO) release. .. Intracellular inhibition of protease digestion In order to inhibit intracellular protease activity, 5 × 106 cells/sample were treated with: Complete™ protease inhibitor tablets; either 1× (1 tablet for every 50 mL of media) or 2× (2 tablets for every 50 mL of media); or treated with antipain (2.0 μg/mL) plus leupeptin (2.0 μg/mL) (both from Sigma-Aldrich, St Louis, MO, USA) for cysteine protease inhibition; or aprotinin (2.0 μg/mL) plus PMSF (100 μg/mL) (both from Sigma-Aldrich) for serine protease inhibition; for to 24 hrs. ..

    Lysis:

    Article Title: Main constraints for RNAi induced by expressed long dsRNA in mouse cells
    Article Snippet: Western blotting 3T3 cells were grown in six-well plates. .. Before collection, the cells were washed with PBS and lysed in lysis buffer (20 mM Hepes [pH 7.8], 100 mM NaCl, 1 mM EDTA [pH 8.0], 0.5% IGEPAL-25%, 1 mM fresh DTT, 0.5 mM PMSF, 1 mM NaF, 0.2 mM Na3 VO4 , supplemented with 2× protease inhibitor cocktail set [Millipore], 2× phosphatase inhibitor cocktail set [Millipore], and RiboLock RNase inhibitor [Thermo Fisher Scientific]). .. Proteins were separated on 10% polyacrylamide gel and transferred to a PVFD membrane (Millipore).

    Protease Inhibitor:

    Article Title: Main constraints for RNAi induced by expressed long dsRNA in mouse cells
    Article Snippet: Western blotting 3T3 cells were grown in six-well plates. .. Before collection, the cells were washed with PBS and lysed in lysis buffer (20 mM Hepes [pH 7.8], 100 mM NaCl, 1 mM EDTA [pH 8.0], 0.5% IGEPAL-25%, 1 mM fresh DTT, 0.5 mM PMSF, 1 mM NaF, 0.2 mM Na3 VO4 , supplemented with 2× protease inhibitor cocktail set [Millipore], 2× phosphatase inhibitor cocktail set [Millipore], and RiboLock RNase inhibitor [Thermo Fisher Scientific]). .. Proteins were separated on 10% polyacrylamide gel and transferred to a PVFD membrane (Millipore).

    Article Title: Ku86 exists as both a full-length and a protease-sensitive natural variant in multiple myeloma cells
    Article Snippet: The reaction mix was incubated at 37°C; and at various time points, aliquots were removed and resolved in a 12.5% SDS-PAGE gel for carbohydrate (CHO) release. .. Intracellular inhibition of protease digestion In order to inhibit intracellular protease activity, 5 × 106 cells/sample were treated with: Complete™ protease inhibitor tablets; either 1× (1 tablet for every 50 mL of media) or 2× (2 tablets for every 50 mL of media); or treated with antipain (2.0 μg/mL) plus leupeptin (2.0 μg/mL) (both from Sigma-Aldrich, St Louis, MO, USA) for cysteine protease inhibition; or aprotinin (2.0 μg/mL) plus PMSF (100 μg/mL) (both from Sigma-Aldrich) for serine protease inhibition; for to 24 hrs. ..

    Electrophoresis:

    Article Title: A Novel Pathway for Mammary Epithelial Cell Invasion Induced by the Helix-Loop-Helix Protein Id-1
    Article Snippet: Briefly, gels consisted of 8 to 10% polyacrylamide and 3 mg of α-casein or gelatin (Sigma)/ml. .. Concentrated conditioned medium was mixed with nonreducing Laemmli sample buffer and incubated at 37°C for 15 min. After electrophoresis, the gels were incubated for 1 h in 2.5% Triton X-100 at room temperature, followed by 24 to 48 h in substrate buffer (100 mM Tris-HCl [pH 7.4]–15 mM CaCl2 ) in the absence or presence of GM6001 (0.2 mM in DMSO; supplied by Glycomed Corporation and obtained from Z. Werb [ ]), EDTA (10 mM), ortho -phenanthroline (1 mM in DMSO; Sigma), PMSF (5 mM), or AEBSF (0.5 mM; Calbiochem). .. The gels were stained with Coomassie blue for 30 min and were destained with 30% methanol–10% acetic acid.

    Inhibition:

    Article Title: Ku86 exists as both a full-length and a protease-sensitive natural variant in multiple myeloma cells
    Article Snippet: The reaction mix was incubated at 37°C; and at various time points, aliquots were removed and resolved in a 12.5% SDS-PAGE gel for carbohydrate (CHO) release. .. Intracellular inhibition of protease digestion In order to inhibit intracellular protease activity, 5 × 106 cells/sample were treated with: Complete™ protease inhibitor tablets; either 1× (1 tablet for every 50 mL of media) or 2× (2 tablets for every 50 mL of media); or treated with antipain (2.0 μg/mL) plus leupeptin (2.0 μg/mL) (both from Sigma-Aldrich, St Louis, MO, USA) for cysteine protease inhibition; or aprotinin (2.0 μg/mL) plus PMSF (100 μg/mL) (both from Sigma-Aldrich) for serine protease inhibition; for to 24 hrs. ..

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  • 99
    Millipore m phenylmethylsulfonylfluoride
    M Phenylmethylsulfonylfluoride, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m phenylmethylsulfonylfluoride/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m phenylmethylsulfonylfluoride - by Bioz Stars, 2021-03
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    Millipore phenylmethylsulfonylfluoride
    Phenylmethylsulfonylfluoride, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylmethylsulfonylfluoride/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethylsulfonylfluoride - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

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