phenylmethylsulfonyl fluoride  (Valiant)

 
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  • 94
    Name:
    Phenylmethylsulfonyl fluoride PMSF 98
    Description:
    Phenylmethylsulfonyl Fluoride Pmsf
    Catalog Number:
    04800263
    Price:
    78.65
    Category:
    Life Sciences Biochemicals
    Applications:
    Inhibitor of trypsin; chymotrypsin and protease
    Size:
    5 g
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    Structured Review

    Valiant phenylmethylsulfonyl fluoride
    Phenylmethylsulfonyl fluoride PMSF 98
    Phenylmethylsulfonyl Fluoride Pmsf
    https://www.bioz.com/result/phenylmethylsulfonyl fluoride/product/Valiant
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethylsulfonyl fluoride - by Bioz Stars, 2021-03
    94/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: The Effects of Select Histidine to Cysteine Mutations on Transcriptional Regulation by E. coli RcnR
    Article Snippet: The cells were harvested after 3 hours by centrifugation (~8000 g for 20 minutes), resuspended in residual media, and frozen at −80 °C. .. The cells were lysed upon thawing in a water bath at 37 °C and treated with 10 μL of a DNAse I solution (10 mg/ml DNAse I, 40% glycerol), 1.5 mM (final concentration) of PMSF (MP Biomedicals) and 5 mM (final concentration) TCEP (Thermo Scientific). .. All chromatographic purifications employed an AKTA-FPLC system (Amersham Biosciences) and were carried out at room temperature.

    Recombinant:

    Article Title: First-In-Class Small Molecule Inhibitors of the Single-Strand DNA Cytosine Deaminase APOBEC3G
    Article Snippet: The fluorescence-based DNA cytosine deamination assay ( - ) was adapted for HTS with purified proteins as follows. .. Recombinant human A3A- or A3G-mycHis proteins were diluted with 50 mM Tris-Cl, pH 7.4, 150 mM NaCl, 10% glycerol, 0.5% triton X-100, 1 mM PMSF (MP Biomedicals), 1 μg/ml Aprotinin (Sigma), 1 μg/ml Leupeptin (MP Biomedicals), and 1μg/ml Pepstatin A (Fisher Scientific) to working concentrations. .. 15 μl enzyme was incubated with 10 pmol ssDNA substrate 5’-6-FAM-AAA-CCC-AAA-GAG-AGA-ATGTGA-TAMRA-3’ (Biosearch Technologies, Inc.) and 0.02 unit of UDG (NEB) diluted in 15 μl of 50 mM Tris.Cl, pH 7.4, 10 mM EDTA for 2 hrs at 37 °C in Nunc 384-well black plates.

    Lysis:

    Article Title: Epidural Stimulation Combined with Triple Gene Therapy for Spinal Cord Injury Treatment
    Article Snippet: Multiplex Cytokine Analysis A simultaneous analysis of the level of 13 cytokines/chemokines was determined in the samples of the spinal cord of intact and experimental animals at 60 days after SCI. .. Freshly frozen spinal cord samples from CS3 segments were ground in a cold RIPA Lysis Buffer (150 mM NaCl, 5 mM EDTA, 50 mM Tris, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) with Protease Inhibitor Cocktail (Sigma-Aldrich, P2714) and 1 mM PMSF, using a FastPrep-24 homogenizer. .. The multiplex analysis was performed using the Porcine Cytokine/Chemokine MILLIPLEX® magnetic bead-based multi-analyte panel containing the following target analytes: GM-CSF, IL-1ra, IL-2, IL-6, IL-10, IL-12, IL-18, TNFα, IFNγ, IL-1β, IL-1α, IL-4, and IL-8 (Millipore).

    Protease Inhibitor:

    Article Title: Epidural Stimulation Combined with Triple Gene Therapy for Spinal Cord Injury Treatment
    Article Snippet: Multiplex Cytokine Analysis A simultaneous analysis of the level of 13 cytokines/chemokines was determined in the samples of the spinal cord of intact and experimental animals at 60 days after SCI. .. Freshly frozen spinal cord samples from CS3 segments were ground in a cold RIPA Lysis Buffer (150 mM NaCl, 5 mM EDTA, 50 mM Tris, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) with Protease Inhibitor Cocktail (Sigma-Aldrich, P2714) and 1 mM PMSF, using a FastPrep-24 homogenizer. .. The multiplex analysis was performed using the Porcine Cytokine/Chemokine MILLIPLEX® magnetic bead-based multi-analyte panel containing the following target analytes: GM-CSF, IL-1ra, IL-2, IL-6, IL-10, IL-12, IL-18, TNFα, IFNγ, IL-1β, IL-1α, IL-4, and IL-8 (Millipore).

    Article Title: Dynamic regulation of alternative splicing and chromatin structure in Drosophila gonads revealed by RNA-seq
    Article Snippet: Using the RPKM values, the correlation coefficient (r) among the technical replicates for each sample was calculated, and this coefficient showed very little variation (r > 0.983) in all samples, consistent with the idea that the RNA-seq method is highly reproducible. .. We dissected 200 pairs of bam testes in cold PBS and grouped in 200 μl PBS which contained protease inhibitor (Roche complete mini, # 11836153001) and 0.5 mM PMSF (MP Biomedicals, #195381); We then added 5.5 μl 37% fresh formaldehyde (Supelco, # 47083-U) and incubated at 37°C for 15mins. ..

    Article Title: α- Linoleanic acid modulates phagocytosis of extracellular Tau and induces microglial migration by actin-remodeling
    Article Snippet: The ALA also improves the migration profile of microglia that aids to the phagocytosis. .. Chemicals and Primary antibodiesLuria-Bertani broth (Himedia); Ampicillin, NaCl, Phenylmethylsulfonylfluoride (PMSF), MgCl2, APS, DMSO, Ethanol (Mol Bio grade), Isopropanol (Mol Bio grade) and methanol (Mol Bio grade) were purchased from MP biomedicals; IPTG and Dithiothreitol (DTT) from Calbiochem; MES, BES, SDS, α- Linolenic acid (ALA) (L2376) from Sigma; EGTA, Protease inhibitor cocktail, Tris base, 40% Acrylamide, TEMED from Invitrogen. .. For cell culture studies, the N9 microglial cell line no. is CVCL-0452, Roswell Park Memorial Institute (RPMI), Fetal Bovine Serum (FBS), Horse serum, Phosphate buffer saline (PBS, cell biology grade), Trypsin-EDTA, Penicillinstreptomycin, RIPA buffer were also purchased from Invitrogen.

    Incubation:

    Article Title: Dynamic regulation of alternative splicing and chromatin structure in Drosophila gonads revealed by RNA-seq
    Article Snippet: Using the RPKM values, the correlation coefficient (r) among the technical replicates for each sample was calculated, and this coefficient showed very little variation (r > 0.983) in all samples, consistent with the idea that the RNA-seq method is highly reproducible. .. We dissected 200 pairs of bam testes in cold PBS and grouped in 200 μl PBS which contained protease inhibitor (Roche complete mini, # 11836153001) and 0.5 mM PMSF (MP Biomedicals, #195381); We then added 5.5 μl 37% fresh formaldehyde (Supelco, # 47083-U) and incubated at 37°C for 15mins. ..

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  • 94
    Valiant pmsf
    Pmsf, supplied by Valiant, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmsf/product/Valiant
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmsf - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

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