Structured Review

Fisher Scientific phenylmethylsulfonyl fluoride
Phenylmethylsulfonyl Fluoride, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phenylmethylsulfonyl fluoride/product/Fisher Scientific
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
phenylmethylsulfonyl fluoride - by Bioz Stars, 2021-04
86/100 stars

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Expressing:

Article Title: Design of Biologically Active Binary Protein 2D Materials
Article Snippet: Designs screeningFor initial screening of the 45 designs, bicistronic plasmids were transformed into BL21 Star (DE3) E. coli. cells (Invitrogen) and cultures grown in 4ml LB media in a 96 well plate setup. .. Protein expression was induced with 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG) for 3 hours at 37°C or 15 hours at 22°C, followed by cell lysis in Tris-buffer (TBS; 25 mM Tris, 300 mM NaCl, 1 mM dithiothreitol (DTT), 1 mM phenylmethylsulfonyl fluoride (PMSF), and lysozyme (0.1mg/ml) using sonication (Fisher Scientific) at 20W for 5min total ‘on’ time, using cycles of 10s on, 10s off. .. Soluble and insoluble fractions were separated by centrifugation and protein expression was evaluated by running both fractions on SDS-PAGE(Bio-Rad) (see ).

Lysis:

Article Title: Design of Biologically Active Binary Protein 2D Materials
Article Snippet: Designs screeningFor initial screening of the 45 designs, bicistronic plasmids were transformed into BL21 Star (DE3) E. coli. cells (Invitrogen) and cultures grown in 4ml LB media in a 96 well plate setup. .. Protein expression was induced with 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG) for 3 hours at 37°C or 15 hours at 22°C, followed by cell lysis in Tris-buffer (TBS; 25 mM Tris, 300 mM NaCl, 1 mM dithiothreitol (DTT), 1 mM phenylmethylsulfonyl fluoride (PMSF), and lysozyme (0.1mg/ml) using sonication (Fisher Scientific) at 20W for 5min total ‘on’ time, using cycles of 10s on, 10s off. .. Soluble and insoluble fractions were separated by centrifugation and protein expression was evaluated by running both fractions on SDS-PAGE(Bio-Rad) (see ).

Article Title: Genome-Wide Analysis of Epstein-Barr Virus Rta DNA Binding
Article Snippet: Briefly, 1 × 106 cells per ChIP were fixed in 1% (wt/vol) formaldehyde (Sigma) for 10 min at 37°C, followed by two washes with PBS. .. Cells were lysed in 1 ml of lysis buffer (50 mM Tris-HCl [pH 8.1], 10 mM EDTA, 1% [wt/vol] SDS, 1 mM phenylmethylsulfonyl fluoride [PMSF], 1 μg/ml leupeptin, and 20 μg/ml aprotinin) for 10 min on ice before extensive sonication using a 550 sonic dismembrator (Fisher Scientific, Pittsburgh, PA). .. After extract clearing by centrifugation, supernatants were diluted 1:10 in dilution buffer (16.7 mM Tris-HCl [pH 8.1], 1.2 mM EDTA, 167 mM NaCl, 1.1% [vol/vol] Triton X-100, 0.01% [wt/vol] SDS, 1 mM PMSF, 1 μg/ml leupeptin, and 20 μg/ml aprotinin) and incubated with protein A-Sepharose 4B (Sigma) for 1 h with rotation at 4°C.

Sonication:

Article Title: Design of Biologically Active Binary Protein 2D Materials
Article Snippet: Designs screeningFor initial screening of the 45 designs, bicistronic plasmids were transformed into BL21 Star (DE3) E. coli. cells (Invitrogen) and cultures grown in 4ml LB media in a 96 well plate setup. .. Protein expression was induced with 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG) for 3 hours at 37°C or 15 hours at 22°C, followed by cell lysis in Tris-buffer (TBS; 25 mM Tris, 300 mM NaCl, 1 mM dithiothreitol (DTT), 1 mM phenylmethylsulfonyl fluoride (PMSF), and lysozyme (0.1mg/ml) using sonication (Fisher Scientific) at 20W for 5min total ‘on’ time, using cycles of 10s on, 10s off. .. Soluble and insoluble fractions were separated by centrifugation and protein expression was evaluated by running both fractions on SDS-PAGE(Bio-Rad) (see ).

Article Title: Genome-Wide Analysis of Epstein-Barr Virus Rta DNA Binding
Article Snippet: Briefly, 1 × 106 cells per ChIP were fixed in 1% (wt/vol) formaldehyde (Sigma) for 10 min at 37°C, followed by two washes with PBS. .. Cells were lysed in 1 ml of lysis buffer (50 mM Tris-HCl [pH 8.1], 10 mM EDTA, 1% [wt/vol] SDS, 1 mM phenylmethylsulfonyl fluoride [PMSF], 1 μg/ml leupeptin, and 20 μg/ml aprotinin) for 10 min on ice before extensive sonication using a 550 sonic dismembrator (Fisher Scientific, Pittsburgh, PA). .. After extract clearing by centrifugation, supernatants were diluted 1:10 in dilution buffer (16.7 mM Tris-HCl [pH 8.1], 1.2 mM EDTA, 167 mM NaCl, 1.1% [vol/vol] Triton X-100, 0.01% [wt/vol] SDS, 1 mM PMSF, 1 μg/ml leupeptin, and 20 μg/ml aprotinin) and incubated with protein A-Sepharose 4B (Sigma) for 1 h with rotation at 4°C.

Imaging:

Article Title: Mechanism of Dephosphorylation of the SR Protein ASF/SF2 By Protein Phosphatase 1
Article Snippet: .. Lysobacter enzymogenes endoproteinase Lys-C (LysC), adenosine triphosphate (ATP), 3-( N -morpholino)propanesulphonic acid (Mops), Tris (hydroxymethyl) aminomethane (Tris), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), phenylmethylsulfonyl fluoride (PMSF), imidazole, sodium vanadate, β-glycerolphosphate (βGP), Tween 20, MgCl2 , NaCl, EDTA, EGTA, glycerol, acetic acid, Kodak imaging film (Biomax MR), TFA, BSA, acetonitrile, and liquid scintillant were obtained from Fisher Scientific. .. Clk-specific inhibitor TG003 was obtained from Sigma-Aldrich.

Protease Inhibitor:

Article Title: Monocyte Caspase-1 Is Released in a Stable, Active High Molecular Weight Complex Distinct from the Unstable Cell Lysate-Activated Caspase-1
Article Snippet: Culture media RPMI-1640 (Corning and Media tech), BEGM (Bronchial Epithelium Growth Medium, Lonza), fetal bovine serum (Atlas), pen-strep (Life technologies). .. Reagents : phosphate buffered saline 0.9% (PBS, Gibco Life Technologies), DTT (dithiothreitol), complete protease inhibitor cocktail (Sigma, P8340), phenylmethylsulfonyl fluoride (PMSF), N-(methoxysuccinyl)-Ala-Ala-Pro-Val-chloromethyl ketone (m-AAPV-cmk), HEPES, Tris base (Fischer Scientific), NaCl, NaF, KCl, MgCl2, EDTA, EGTA, Triton X-100, Na-deoxycholate. .. Agonists, substrates, and inhibitors Endotoxin (LPS, from E . coli strain 0111:B4; Alexis Biochemicals, San Diego), ATP, Ac-Trp-Glu-His-Asp-7-Amino-4-trifluoromethylcoumarin (afc) (WEHD-afc, Calbiochem), Ac-Tyr-Val-Ala-Asp-chloromethylketone (cmk) (YVAD-cmk, Calbiochem), Biotin-YVAD-cmk (Anaspec, USA), recombinant human IL-1β (His-tag, Sino Biological, China).

other:

Article Title: Isoform-specific therapeutic control of sulfonation in humans
Article Snippet: Ampicillin, HEPES, KCl, KOH, MgCl2 and phenylmethylsulfonyl fluoride (PMSF) were purchased from Fisher Scientific.

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    Fisher Scientific pmsf
    Pmsf, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmsf/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmsf - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Fisher Scientific phenylmethylsulfonyl fluoride pmsf
    Phenylmethylsulfonyl Fluoride Pmsf, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylmethylsulfonyl fluoride pmsf/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethylsulfonyl fluoride pmsf - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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