Structured Review

Beijing Solarbio Science phenylmethylsulfonyl fluoride
Phenylmethylsulfonyl Fluoride, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phenylmethylsulfonyl fluoride/product/Beijing Solarbio Science
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
phenylmethylsulfonyl fluoride - by Bioz Stars, 2021-03
86/100 stars

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Related Articles

Western Blot:

Article Title: Enhanced plasma miR-26a-5p promotes the progression of bladder cancer via targeting PTEN
Article Snippet: .. Western blot analysis HT1376 cells were treated with radioimmunoprecipitation assay buffer (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) containing 1% (v/v) phenylmethylsulfonyl fluoride (Beijing Solarbio Science & Technology Co., Ltd.), 0.3% (v/v) protease inhibitor (Sigma-Aldrich; Merck KGaA) and 0.1% (v/v) phosphorylated proteinase inhibitor (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). .. A BCA protein assay kit (Pierce; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Treadmill exercise promotes neurogenesis and myelin repair via upregulating Wnt/β-catenin signaling pathways in the juvenile brain following focal cerebral ischemia/reperfusion
Article Snippet: .. Reagents Anti-Wnt3a (1:1,000; EMD Millipore), anti-β-catenin (1:5,000; Abcam), anti-BDNF (1:1,000; Abcam), anti-myelin basic protein (MBP; 1:1,000; Abcam), anti-Dcx (1:200; Abcam), anti-NESTIN (1:200; Abcam), anti-GAPDH (1:2,000; Abcam), anti-Lamin B1 (1;1,000; Abcam), XAV939 (Selleck Chemicals), 2,3,5-triphenyl tetrazolium chloride (TTC; Sigma-Aldrich; Merck KGaA), phenylmethylsulfonyl-fluoride (Beijing Solarbio Science & Technology Co., Ltd.), Clarity™ Western ECL Substrate kit (Bio-Rad Laboratories, Inc.), Alexa Fluor 488 AffiniPure Donkey Anti-Mouse IgG and Alexa Fluor 594 AffiniPure Goat Anti-Rabbit IgG (1:200; Yesen Bio), 4,6-diamidino-2-phenylindole (DAPI; Beyotime Institute of Biotechnology). ..

Radio Immunoprecipitation:

Article Title: Enhanced plasma miR-26a-5p promotes the progression of bladder cancer via targeting PTEN
Article Snippet: .. Western blot analysis HT1376 cells were treated with radioimmunoprecipitation assay buffer (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) containing 1% (v/v) phenylmethylsulfonyl fluoride (Beijing Solarbio Science & Technology Co., Ltd.), 0.3% (v/v) protease inhibitor (Sigma-Aldrich; Merck KGaA) and 0.1% (v/v) phosphorylated proteinase inhibitor (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). .. A BCA protein assay kit (Pierce; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: CKAP2 expression is associated with glioma tumor growth and acts as a prognostic factor in high-grade glioma
Article Snippet: .. Total proteins were extracted using radioimmunoprecipitation assay buffer (Cell Signaling Technology, Inc., Danvers, MA, USA) supplemented with phenylmethylsulfonyl fluoride (1 mM; Beijing Solarbio Science & Technology Co., Ltd., Beijing, China). .. Pierce Bicinchoninic Acid Protein Assay kit (Thermo Fisher Scientific, Inc.) was used to quantify protein concentration.

Article Title: Effect of 1α, 25-dihydroxyvitamin D3 on the osteogenic differentiation of human periodontal ligament stem cells and the underlying regulatory mechanism
Article Snippet: The primary monoclonal antibodies used included rabbit anti-human TAZ (cat. no. cst70148; Cell Signaling Technology, Inc.; 1:1,000), rabbit anti-human ALP (cat. no. ab108337; Abcam, Cambridge, UK; 1:20,000), mouse anti-human OPN (cat. no. sc-21742; Santa Cruz Biotechnology, Inc; 1:500), rabbit anti-human PCNA (cat. no. WL0341c; Wanlei Biotechnology Co., Ltd., Shanghai, China; 1:1,000), and anti-human GAPDH monoclonal (cat. no. HRP-60004, ProteinTech Group, Inc., Chicago, IL, USA; 1:20,000). .. The cells were lysed in radioimmunoprecipitation assay buffer (Beijing Solarbio Science & Technology Co., Ltd.) containing 1% phenylmethylsulfonyl fluoride (Beijing Solarbio Science & Technology Co., Ltd.). .. The protein concentration was quantified using the bicin-choninic acid (Beijing Solarbio Science & Technology Co., Ltd.) method.

Protease Inhibitor:

Article Title: Enhanced plasma miR-26a-5p promotes the progression of bladder cancer via targeting PTEN
Article Snippet: .. Western blot analysis HT1376 cells were treated with radioimmunoprecipitation assay buffer (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) containing 1% (v/v) phenylmethylsulfonyl fluoride (Beijing Solarbio Science & Technology Co., Ltd.), 0.3% (v/v) protease inhibitor (Sigma-Aldrich; Merck KGaA) and 0.1% (v/v) phosphorylated proteinase inhibitor (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). .. A BCA protein assay kit (Pierce; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Sonication:

Article Title: Quantification of the CM-Dil-labeled human umbilical cord mesenchymal stem cells migrated to the dual injured uterus in SD rat
Article Snippet: The relative expression was calculated using the 2–ΔΔCT method. .. The tissues above were quickly removed and homogenized using RIPA buffer and phenylmethylsulfonyl fluoride (Beijing Solarbio Science & Technology Co., Ltd.) by sonication. .. Proteins from the supernatant were separated by electrophoresed in 10% SDS-PAGE gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Beijing Solarbio Science & Technology Co., Ltd.).

Lysis:

Article Title: Bisphenol A disturbs transcription of steroidogenic genes in ovary of rare minnow Gobiocypris rarus via the abnormal DNA and histone methylation.
Article Snippet: Increasing studies have established the toxic effects of BPA on development and reproduction in animals. .. Increasing studies have established the toxic effects of BPA on development and reproduction in animals. .. Increasing studies have established the toxic effects of BPA on development and reproduction in animals.

Article Title: Tetramethylpyrazine induces differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells in vitro
Article Snippet: .. The following reagents were obtained commercially: TMP (20 mg; purity: HPLC ≥98%; batch no. 120919 Chengdu Preferred Biological Technology Co., Ltd, Chengdu, China); low glucose Dulbecco's Modified Eagle's Medium (L-DMEM)/F-12, high glucose-DMEM (H-DMEM)/F-12 and fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA); trypsin (Amresco, LLC, Solon, OH, USA); EDTA and DMSO (Sigma-Aldrich, St. Louis, MO, USA); Triton X-100 (Sinopharm Chemical Reagent Co., Ltd., Beijing, China); penicillin-streptomycin (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); paraformaldehyde (Tianjin Institute of Chemical Preparations, Tianjin, China); epidermal growth factor (EGF) (human EGF, PHG0314; Beijing Jiamei North Biological Technology Co., Ltd., Beijing, China); fluorescein isothiocyanate (FITC)-CD19, FITC-CD34, phycoerythrin (PE)-CD11b, PE-CD73, PE-CD90, PE-CD45, PE-CD105 and glial fibrillary acidic protein (GFAP) (BD Biosciences, Franklin Lakes, NJ, USA); neurofilament protein (NF-H), neuron-specific enolase (NSE) (Cell Signaling Technology, Inc., Danvers, MA, USA); anti-rabbit immunoglobulin G (IgG) (1:2,000; Affinity Biosciences, Cincinnati, OH, USA); PS immunohistochemistry kit (Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China); Taq polymerase chain reaction (PCR) star mix (Beijing GenStar Biosolutions Co., Ltd., Beijing, China); EasyScript First-Strand cDNA synthesis supermix (TransGen Biotech Co., Ltd., Beijing, China); cell RIPA lysis buffer and phenylmethylsulfonyl fluoride (Beijing Solarbio Science & Technology Co., Ltd.). .. Isolation and culture of hUMSCs Umbilical cords were placed in H-DMEM/F-12 culture medium under aseptic conditions, stored at 4°C and then timely transported to a cell culture room to carry out the following steps.

High Performance Liquid Chromatography:

Article Title: Tetramethylpyrazine induces differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells in vitro
Article Snippet: .. The following reagents were obtained commercially: TMP (20 mg; purity: HPLC ≥98%; batch no. 120919 Chengdu Preferred Biological Technology Co., Ltd, Chengdu, China); low glucose Dulbecco's Modified Eagle's Medium (L-DMEM)/F-12, high glucose-DMEM (H-DMEM)/F-12 and fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA); trypsin (Amresco, LLC, Solon, OH, USA); EDTA and DMSO (Sigma-Aldrich, St. Louis, MO, USA); Triton X-100 (Sinopharm Chemical Reagent Co., Ltd., Beijing, China); penicillin-streptomycin (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); paraformaldehyde (Tianjin Institute of Chemical Preparations, Tianjin, China); epidermal growth factor (EGF) (human EGF, PHG0314; Beijing Jiamei North Biological Technology Co., Ltd., Beijing, China); fluorescein isothiocyanate (FITC)-CD19, FITC-CD34, phycoerythrin (PE)-CD11b, PE-CD73, PE-CD90, PE-CD45, PE-CD105 and glial fibrillary acidic protein (GFAP) (BD Biosciences, Franklin Lakes, NJ, USA); neurofilament protein (NF-H), neuron-specific enolase (NSE) (Cell Signaling Technology, Inc., Danvers, MA, USA); anti-rabbit immunoglobulin G (IgG) (1:2,000; Affinity Biosciences, Cincinnati, OH, USA); PS immunohistochemistry kit (Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China); Taq polymerase chain reaction (PCR) star mix (Beijing GenStar Biosolutions Co., Ltd., Beijing, China); EasyScript First-Strand cDNA synthesis supermix (TransGen Biotech Co., Ltd., Beijing, China); cell RIPA lysis buffer and phenylmethylsulfonyl fluoride (Beijing Solarbio Science & Technology Co., Ltd.). .. Isolation and culture of hUMSCs Umbilical cords were placed in H-DMEM/F-12 culture medium under aseptic conditions, stored at 4°C and then timely transported to a cell culture room to carry out the following steps.

Modification:

Article Title: Tetramethylpyrazine induces differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells in vitro
Article Snippet: .. The following reagents were obtained commercially: TMP (20 mg; purity: HPLC ≥98%; batch no. 120919 Chengdu Preferred Biological Technology Co., Ltd, Chengdu, China); low glucose Dulbecco's Modified Eagle's Medium (L-DMEM)/F-12, high glucose-DMEM (H-DMEM)/F-12 and fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA); trypsin (Amresco, LLC, Solon, OH, USA); EDTA and DMSO (Sigma-Aldrich, St. Louis, MO, USA); Triton X-100 (Sinopharm Chemical Reagent Co., Ltd., Beijing, China); penicillin-streptomycin (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); paraformaldehyde (Tianjin Institute of Chemical Preparations, Tianjin, China); epidermal growth factor (EGF) (human EGF, PHG0314; Beijing Jiamei North Biological Technology Co., Ltd., Beijing, China); fluorescein isothiocyanate (FITC)-CD19, FITC-CD34, phycoerythrin (PE)-CD11b, PE-CD73, PE-CD90, PE-CD45, PE-CD105 and glial fibrillary acidic protein (GFAP) (BD Biosciences, Franklin Lakes, NJ, USA); neurofilament protein (NF-H), neuron-specific enolase (NSE) (Cell Signaling Technology, Inc., Danvers, MA, USA); anti-rabbit immunoglobulin G (IgG) (1:2,000; Affinity Biosciences, Cincinnati, OH, USA); PS immunohistochemistry kit (Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China); Taq polymerase chain reaction (PCR) star mix (Beijing GenStar Biosolutions Co., Ltd., Beijing, China); EasyScript First-Strand cDNA synthesis supermix (TransGen Biotech Co., Ltd., Beijing, China); cell RIPA lysis buffer and phenylmethylsulfonyl fluoride (Beijing Solarbio Science & Technology Co., Ltd.). .. Isolation and culture of hUMSCs Umbilical cords were placed in H-DMEM/F-12 culture medium under aseptic conditions, stored at 4°C and then timely transported to a cell culture room to carry out the following steps.

Immunohistochemistry:

Article Title: Tetramethylpyrazine induces differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells in vitro
Article Snippet: .. The following reagents were obtained commercially: TMP (20 mg; purity: HPLC ≥98%; batch no. 120919 Chengdu Preferred Biological Technology Co., Ltd, Chengdu, China); low glucose Dulbecco's Modified Eagle's Medium (L-DMEM)/F-12, high glucose-DMEM (H-DMEM)/F-12 and fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA); trypsin (Amresco, LLC, Solon, OH, USA); EDTA and DMSO (Sigma-Aldrich, St. Louis, MO, USA); Triton X-100 (Sinopharm Chemical Reagent Co., Ltd., Beijing, China); penicillin-streptomycin (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); paraformaldehyde (Tianjin Institute of Chemical Preparations, Tianjin, China); epidermal growth factor (EGF) (human EGF, PHG0314; Beijing Jiamei North Biological Technology Co., Ltd., Beijing, China); fluorescein isothiocyanate (FITC)-CD19, FITC-CD34, phycoerythrin (PE)-CD11b, PE-CD73, PE-CD90, PE-CD45, PE-CD105 and glial fibrillary acidic protein (GFAP) (BD Biosciences, Franklin Lakes, NJ, USA); neurofilament protein (NF-H), neuron-specific enolase (NSE) (Cell Signaling Technology, Inc., Danvers, MA, USA); anti-rabbit immunoglobulin G (IgG) (1:2,000; Affinity Biosciences, Cincinnati, OH, USA); PS immunohistochemistry kit (Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China); Taq polymerase chain reaction (PCR) star mix (Beijing GenStar Biosolutions Co., Ltd., Beijing, China); EasyScript First-Strand cDNA synthesis supermix (TransGen Biotech Co., Ltd., Beijing, China); cell RIPA lysis buffer and phenylmethylsulfonyl fluoride (Beijing Solarbio Science & Technology Co., Ltd.). .. Isolation and culture of hUMSCs Umbilical cords were placed in H-DMEM/F-12 culture medium under aseptic conditions, stored at 4°C and then timely transported to a cell culture room to carry out the following steps.

Polymerase Chain Reaction:

Article Title: Tetramethylpyrazine induces differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells in vitro
Article Snippet: .. The following reagents were obtained commercially: TMP (20 mg; purity: HPLC ≥98%; batch no. 120919 Chengdu Preferred Biological Technology Co., Ltd, Chengdu, China); low glucose Dulbecco's Modified Eagle's Medium (L-DMEM)/F-12, high glucose-DMEM (H-DMEM)/F-12 and fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA); trypsin (Amresco, LLC, Solon, OH, USA); EDTA and DMSO (Sigma-Aldrich, St. Louis, MO, USA); Triton X-100 (Sinopharm Chemical Reagent Co., Ltd., Beijing, China); penicillin-streptomycin (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); paraformaldehyde (Tianjin Institute of Chemical Preparations, Tianjin, China); epidermal growth factor (EGF) (human EGF, PHG0314; Beijing Jiamei North Biological Technology Co., Ltd., Beijing, China); fluorescein isothiocyanate (FITC)-CD19, FITC-CD34, phycoerythrin (PE)-CD11b, PE-CD73, PE-CD90, PE-CD45, PE-CD105 and glial fibrillary acidic protein (GFAP) (BD Biosciences, Franklin Lakes, NJ, USA); neurofilament protein (NF-H), neuron-specific enolase (NSE) (Cell Signaling Technology, Inc., Danvers, MA, USA); anti-rabbit immunoglobulin G (IgG) (1:2,000; Affinity Biosciences, Cincinnati, OH, USA); PS immunohistochemistry kit (Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China); Taq polymerase chain reaction (PCR) star mix (Beijing GenStar Biosolutions Co., Ltd., Beijing, China); EasyScript First-Strand cDNA synthesis supermix (TransGen Biotech Co., Ltd., Beijing, China); cell RIPA lysis buffer and phenylmethylsulfonyl fluoride (Beijing Solarbio Science & Technology Co., Ltd.). .. Isolation and culture of hUMSCs Umbilical cords were placed in H-DMEM/F-12 culture medium under aseptic conditions, stored at 4°C and then timely transported to a cell culture room to carry out the following steps.

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    Beijing Solarbio Science phenylmethylsulfonyl fluoride
    Phenylmethylsulfonyl Fluoride, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylmethylsulfonyl fluoride/product/Beijing Solarbio Science
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethylsulfonyl fluoride - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Beijing Solarbio Science phenylmethanesulfonyl fluoride
    Phenylmethanesulfonyl Fluoride, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylmethanesulfonyl fluoride/product/Beijing Solarbio Science
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethanesulfonyl fluoride - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Beijing Solarbio Science pmsf
    Pmsf, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmsf/product/Beijing Solarbio Science
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmsf - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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