phenylmethylsulfonyl fluoride pmsf  (Cell Signaling Technology Inc)

 
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    Name:
    PMSF
    Description:
    Phenylmethanesulfonyl Fluoride PMSF is an inhibitor of serine proteases such as trypsin chymotrypsin thrombin and papain It is routinely added as a supplement to lysis buffers just prior to lysis to prevent protease degradation Cell Signaling Technology recommends adding PMSF at 1 mM to Cell Lysis Buffer 9803 and RIPA Buffer 9806 Molecular Formula C7H7FO2S Molecular Weight 174 19 CAS 329 98 6
    Catalog Number:
    8553
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    Buffers Dyes
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    Structured Review

    Cell Signaling Technology Inc phenylmethylsulfonyl fluoride pmsf
    Phenylmethanesulfonyl Fluoride PMSF is an inhibitor of serine proteases such as trypsin chymotrypsin thrombin and papain It is routinely added as a supplement to lysis buffers just prior to lysis to prevent protease degradation Cell Signaling Technology recommends adding PMSF at 1 mM to Cell Lysis Buffer 9803 and RIPA Buffer 9806 Molecular Formula C7H7FO2S Molecular Weight 174 19 CAS 329 98 6
    https://www.bioz.com/result/phenylmethylsulfonyl fluoride pmsf/product/Cell Signaling Technology Inc
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethylsulfonyl fluoride pmsf - by Bioz Stars, 2021-03
    98/100 stars

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    Related Articles

    Lysis:

    Article Title: Epstein–Barr Virus MicroRNAs are Expressed in Patients with Chronic Lymphocytic Leukemia and Correlate with Overall Survival
    Article Snippet: Empty lentivirus (LVEV, lentivirus empty vector) was used as a negative control for the experiments. .. After 96 h from infections, the protein extracts were prepared in ice-cold RIPA lysis buffer 1 × (Santa Cruz Biotechnology) containing phenylmethylsulfonyl fluoride (PMSF), sodium orthovanadate and protease inhibitor cocktail, or in 1 × Lysis buffer (Cell Signaling) supplemented with protease and phosphatase inhibitor (Sigma-Aldrich) according to the manufacturer's instructions. .. Total protein (50 μg) was electrophoresed on Criterion TGX Stain Free Precast Gels (Bio-Rad Laboratories) and electroblotted onto pure PVDF or nitrocellulose membranes (Trans-Blot Transfer Turbo midi-format 0.2 μm; Bio-Rad Laboratories) with the Trans Blot Turbo System (Bio-Rad Laboratories).

    Article Title: Identification of Key Candidate Proteins and Pathways Associated with Temozolomide Resistance in Glioblastoma Based on Subcellular Proteomics and Bioinformatical Analysis
    Article Snippet: The method of trypan blue staining was described in our previous study [ ]. .. Nuclear and cytoplasmic fractions were generated according to the protocol as follows: 2 × 107 cells were collected and suspended in 300–500 μ l RLN lysis buffer (Qiagen, Germany) with 1% PMSF, kept on ice for 20 min, and centrifuged for 10 min at 3000 ×g, 4°C; the clear supernatant was the cytoplasmic extractions; the nucleus precipitate was washed with RLN lysis buffer 3 times and the supernatant was discarded; the nucleus precipitate was suspended in 100 μ l RIPA lysis buffer (Cell Signaling Technology, USA) with 1% PMSF, with sonication on ice for 1 min; the suspension was centrifuged at 12000 ×g for 20 min at 4°C; the clear supernatant was the nuclear extractions. .. The validation of purity of the extractions was indicated for western blots probed with antibodies against two fractionation controls (Histone H3 for nucleus extraction, Beyotime, China; dilution 1 : 1000; GAPDH for cytoplasma extraction, Jetway Biotech Co., Ltd., China; dilution 1 : 2000).

    Article Title: Dexmedetomidine prevents cognitive decline by enhancing resolution of HMGB1-induced inflammation through a vagomimetic action in mice
    Article Snippet: Results are expressed as fold-change compared with that measured in control mice that did not receive any intervention. .. Tissues were homogenized with RIPA Lysis Buffer (Cell Signaling Technology) plus protease inhibitor (Halt Protease Inhibitor Single-Use Cocktail, Thermo Fisher Scientific) and phenylmethanesulfonyl fluoride (PMSF, Cell Signaling Technology) and sonicated. .. Protein concentration was assayed with Pierce BCA Protein Assay kit (Thermo Prod).

    Article Title: Microenvironment-sensing, nanocarrier-mediated delivery of combination chemotherapy for pancreatic cancer
    Article Snippet: .. Cells were directly lysed in culture plates by the addition of a lysis buffer supplemented with 1 mM PMSF and 1X protease/phosphatase inhibitor cocktail (Cell Signalling). ..

    Article Title: The homeoprotein Dlx5 drives murine T-cell lymphomagenesis by directly transactivating Notch and upregulating Akt signaling
    Article Snippet: Lymphoma cell lines from Lck-Dlx5 mice were seeded at 1 × 104 cells/well in 96-well plates and treated with inhibitors for 24 or 48 h. OD value at 490 nm was measured 2–4 h after incubation of cells with 10% MTS using a 96-well microplate reader (BioRad). .. Western blot analysis Protein from lymphoma cell lines from Lck-Dlx5 mice was extracted with cell lysis buffer supplemented with 2 mM PMSF (Cell Signaling); 50 μg/well proteins were loaded into Tris-Glycine SDS-PAGE gels (Invitrogen) and transferred onto PVDF membranes (Millipore). ..

    Protease Inhibitor:

    Article Title: Epstein–Barr Virus MicroRNAs are Expressed in Patients with Chronic Lymphocytic Leukemia and Correlate with Overall Survival
    Article Snippet: Empty lentivirus (LVEV, lentivirus empty vector) was used as a negative control for the experiments. .. After 96 h from infections, the protein extracts were prepared in ice-cold RIPA lysis buffer 1 × (Santa Cruz Biotechnology) containing phenylmethylsulfonyl fluoride (PMSF), sodium orthovanadate and protease inhibitor cocktail, or in 1 × Lysis buffer (Cell Signaling) supplemented with protease and phosphatase inhibitor (Sigma-Aldrich) according to the manufacturer's instructions. .. Total protein (50 μg) was electrophoresed on Criterion TGX Stain Free Precast Gels (Bio-Rad Laboratories) and electroblotted onto pure PVDF or nitrocellulose membranes (Trans-Blot Transfer Turbo midi-format 0.2 μm; Bio-Rad Laboratories) with the Trans Blot Turbo System (Bio-Rad Laboratories).

    Article Title: Th22 cells increase in poor prognosis multiple myeloma and promote tumor cell growth and survival
    Article Snippet: Western blot assay MM1S cells were (1) left untreated or treated for 30 min with IL-22 (200 ng/mL) or IL-6 (100 ng/mL) or (2) left untreated or treated for 24 h with Dx (1 μmol/L) with or without IL-22 (50 ng/mL) or IL-6 (50 ng/mL). .. Cells were then lysed in RIPA buffer (Cell Signaling Technology) containing 100 mmol/L phenyl methane sulfonyl fluoride (PMSF), 100 mmol/L dithiothreitol (DTT), and protease inhibitor cocktail (Cell Signaling Technology). .. Immunohistochemistry Bone marrow biopsy samples were fixed, decalcified, and processed as described in Pruneri et al.

    Article Title: Interstitial flow promotes macrophage polarization toward an M2 phenotype
    Article Snippet: Collagen I gel containing cells was washed two times with ice-cold 1X phosphate-buffered saline and transferred into an Eppendorf tube. .. RIPA buffer (Cell Signaling) containing protease inhibitor (Sigma) and PMSF (Cell Signaling) was used to extract the cell lysates. ..

    Article Title: Dexmedetomidine prevents cognitive decline by enhancing resolution of HMGB1-induced inflammation through a vagomimetic action in mice
    Article Snippet: Results are expressed as fold-change compared with that measured in control mice that did not receive any intervention. .. Tissues were homogenized with RIPA Lysis Buffer (Cell Signaling Technology) plus protease inhibitor (Halt Protease Inhibitor Single-Use Cocktail, Thermo Fisher Scientific) and phenylmethanesulfonyl fluoride (PMSF, Cell Signaling Technology) and sonicated. .. Protein concentration was assayed with Pierce BCA Protein Assay kit (Thermo Prod).

    Generated:

    Article Title: Identification of Key Candidate Proteins and Pathways Associated with Temozolomide Resistance in Glioblastoma Based on Subcellular Proteomics and Bioinformatical Analysis
    Article Snippet: The method of trypan blue staining was described in our previous study [ ]. .. Nuclear and cytoplasmic fractions were generated according to the protocol as follows: 2 × 107 cells were collected and suspended in 300–500 μ l RLN lysis buffer (Qiagen, Germany) with 1% PMSF, kept on ice for 20 min, and centrifuged for 10 min at 3000 ×g, 4°C; the clear supernatant was the cytoplasmic extractions; the nucleus precipitate was washed with RLN lysis buffer 3 times and the supernatant was discarded; the nucleus precipitate was suspended in 100 μ l RIPA lysis buffer (Cell Signaling Technology, USA) with 1% PMSF, with sonication on ice for 1 min; the suspension was centrifuged at 12000 ×g for 20 min at 4°C; the clear supernatant was the nuclear extractions. .. The validation of purity of the extractions was indicated for western blots probed with antibodies against two fractionation controls (Histone H3 for nucleus extraction, Beyotime, China; dilution 1 : 1000; GAPDH for cytoplasma extraction, Jetway Biotech Co., Ltd., China; dilution 1 : 2000).

    Sonication:

    Article Title: Identification of Key Candidate Proteins and Pathways Associated with Temozolomide Resistance in Glioblastoma Based on Subcellular Proteomics and Bioinformatical Analysis
    Article Snippet: The method of trypan blue staining was described in our previous study [ ]. .. Nuclear and cytoplasmic fractions were generated according to the protocol as follows: 2 × 107 cells were collected and suspended in 300–500 μ l RLN lysis buffer (Qiagen, Germany) with 1% PMSF, kept on ice for 20 min, and centrifuged for 10 min at 3000 ×g, 4°C; the clear supernatant was the cytoplasmic extractions; the nucleus precipitate was washed with RLN lysis buffer 3 times and the supernatant was discarded; the nucleus precipitate was suspended in 100 μ l RIPA lysis buffer (Cell Signaling Technology, USA) with 1% PMSF, with sonication on ice for 1 min; the suspension was centrifuged at 12000 ×g for 20 min at 4°C; the clear supernatant was the nuclear extractions. .. The validation of purity of the extractions was indicated for western blots probed with antibodies against two fractionation controls (Histone H3 for nucleus extraction, Beyotime, China; dilution 1 : 1000; GAPDH for cytoplasma extraction, Jetway Biotech Co., Ltd., China; dilution 1 : 2000).

    Article Title: Dexmedetomidine prevents cognitive decline by enhancing resolution of HMGB1-induced inflammation through a vagomimetic action in mice
    Article Snippet: Results are expressed as fold-change compared with that measured in control mice that did not receive any intervention. .. Tissues were homogenized with RIPA Lysis Buffer (Cell Signaling Technology) plus protease inhibitor (Halt Protease Inhibitor Single-Use Cocktail, Thermo Fisher Scientific) and phenylmethanesulfonyl fluoride (PMSF, Cell Signaling Technology) and sonicated. .. Protein concentration was assayed with Pierce BCA Protein Assay kit (Thermo Prod).

    Western Blot:

    Article Title: The homeoprotein Dlx5 drives murine T-cell lymphomagenesis by directly transactivating Notch and upregulating Akt signaling
    Article Snippet: Lymphoma cell lines from Lck-Dlx5 mice were seeded at 1 × 104 cells/well in 96-well plates and treated with inhibitors for 24 or 48 h. OD value at 490 nm was measured 2–4 h after incubation of cells with 10% MTS using a 96-well microplate reader (BioRad). .. Western blot analysis Protein from lymphoma cell lines from Lck-Dlx5 mice was extracted with cell lysis buffer supplemented with 2 mM PMSF (Cell Signaling); 50 μg/well proteins were loaded into Tris-Glycine SDS-PAGE gels (Invitrogen) and transferred onto PVDF membranes (Millipore). ..

    Mouse Assay:

    Article Title: The homeoprotein Dlx5 drives murine T-cell lymphomagenesis by directly transactivating Notch and upregulating Akt signaling
    Article Snippet: Lymphoma cell lines from Lck-Dlx5 mice were seeded at 1 × 104 cells/well in 96-well plates and treated with inhibitors for 24 or 48 h. OD value at 490 nm was measured 2–4 h after incubation of cells with 10% MTS using a 96-well microplate reader (BioRad). .. Western blot analysis Protein from lymphoma cell lines from Lck-Dlx5 mice was extracted with cell lysis buffer supplemented with 2 mM PMSF (Cell Signaling); 50 μg/well proteins were loaded into Tris-Glycine SDS-PAGE gels (Invitrogen) and transferred onto PVDF membranes (Millipore). ..

    SDS Page:

    Article Title: The homeoprotein Dlx5 drives murine T-cell lymphomagenesis by directly transactivating Notch and upregulating Akt signaling
    Article Snippet: Lymphoma cell lines from Lck-Dlx5 mice were seeded at 1 × 104 cells/well in 96-well plates and treated with inhibitors for 24 or 48 h. OD value at 490 nm was measured 2–4 h after incubation of cells with 10% MTS using a 96-well microplate reader (BioRad). .. Western blot analysis Protein from lymphoma cell lines from Lck-Dlx5 mice was extracted with cell lysis buffer supplemented with 2 mM PMSF (Cell Signaling); 50 μg/well proteins were loaded into Tris-Glycine SDS-PAGE gels (Invitrogen) and transferred onto PVDF membranes (Millipore). ..

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  • 99
    Cell Signaling Technology Inc pmsf
    Pmsf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmsf/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmsf - by Bioz Stars, 2021-03
    99/100 stars
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