phenylmethanesulfonyl fluoride pmsf (Amresco)

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Amresco phenylmethanesulfonyl fluoride pmsf
Phenylmethanesulfonyl Fluoride Pmsf, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phenylmethanesulfonyl fluoride pmsf/product/Amresco
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

phenylmethanesulfonyl fluoride pmsf - by Bioz Stars, 2021-04

86/100 stars

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Article Title: ATP Induces Disruption of Tight Junction Proteins via IL-1 Beta-Dependent MMP-9 Activation of Human Blood-Brain Barrier In Vitro
Article Snippet: Fetal bovine serum (FBS) was obtained from GIBCO (Rockville, MD, USA); phenylmethanesulfonyl fluoride (PMSF) was acquired from Amresco (Solon, OH, USA); the different cocktails were purchased from Yuanye Biotech (Shanghai, China); skimmed milk was obtained from Yili Industrial Group Co. Ltd. (Beijing, China); and anti-β -actin and other materials were purchased from Jiamay Biolab (Beijing, China).

Article Title: PRRT2 Mutant Leads to Dysfunction of Glutamate Signaling
Article Snippet: Phenylmethanesulfonyl fluoride (PMSF) and Triton X-100 were purchased from Amresco (Solon, OH, USA).

Lysis:

Article Title: Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly
Article Snippet: The proteins above were all expressed in Escherichia coli BL21 DE3 and purified as previously described , , . .. For GST-tagged proteins, cell pellets from 1 L of culture were resuspended with 50 mM Na2 HPO4 -NaH2 PO4 , pH 7.6, 300 mM NaCl, 0.5% Triton X-100 (Sigma; lysis buffer A) supplied with 1 mM phenylmethanesulfonyl fluoride (PMSF, Amresco) and 5 mM 2-mercaptoethanol (2-ME, Amresco). ..

Article Title: A Stimulation Function of Synaptotagmin-1 in Ternary SNARE Complex Formation Dependent on Munc18 and Munc13
Article Snippet: Cultured cells were harvested by centrifuging at 4200 rpm in a J6-MI centrifuge equipped with JS-4.2 rotor (Beckman Coulter) at 4°C. .. For GST-tagged proteins, cell pellets from 1 L of culture were resuspended with 50 mM Na2 HPO4 -NaH2 PO4 pH 7.6, 300 mM NaCl, 10% (v/v) glycerol, 0.5% Triton X-100 (Sigma; lysis buffer A) supplied with 1 mM phenylmethanesulfonyl fluoride (PMSF, Amresco) and 5 mM 2-mercaptoethanol (2-ME, Amresco). ..

Article Title: MiR-27b-3p Inhibition Enhances Browning of Epididymal Fat in High-Fat Diet Induced Obese Mice
Article Snippet: .. Total protein was extracted with radioimmunoprecipitation buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% [v/v] Nonidet P-40, 1 mM EDTA, 1 mM NaF, 10 μg/mL aprotinin, 10 μM leupeptin, and 1 mM phenylmethanesulfonyl fluoride (PMSF); Amresco, OH, USA) and allowed to stand on ice for 30 min to permit lysis. ..

Western Blot:

Article Title: Novel noninvasive quantification of penile corpus cavernosum lesions in hyperlipidemia-induced erectile dysfunction in rabbits by two-dimensional shear-wave elastography
Article Snippet: The slides were photographed using a Nikon Microphot-FXA microscope (Nikon Co. Ltd., Kogaku, Tokyo, Japan). .. Western blot analysis Frozen samples were washed three times in PBS (Beyotime Biotechnology Co. Ltd., Shanghai, China), cut into small pieces, then suspended in 1 mmol l−1 phenylmethanesulfonyl fluoride (PMSF; Amresco Inc., Huston, TX, USA) and radioimmunoprecipitation (RIPA) buffer (Beyotime Biotechnology Co. Ltd.) on ice. .. Thirty minutes later, the lysed homogenate was centrifuged at 10 000 g for 5 min and the supernatant was collected for protein measurement using a BCA kit (Beyotime Biotechnology Co. Ltd.).

Incubation:

Article Title: Vitamin A Supplementation in Early Life Enhances the Intestinal Immune Response of Rats with Gestational Vitamin A Deficiency by Increasing the Number of Immune Cells
Article Snippet: Detection of IgA in the feces The concentration of secretory IgA (sIgA) in the intestine was measured using an ELISA kit (Bethyl Lab, Inc., USA) according to the manufacturer's instructions. .. Briefly, according to the method described by Frossard , 250 mg of feces obtained from the ileocecum of rats was resuspended and incubated in 1 ml of 0.01 M PBS containing 1% fetal bovine serum (FBS) and 0.1 mM phenylmethanesulfonyl fluoride (PMSF, Solarbio, Amresco) for 1 hr at room temperature. .. The concentration of total sIgA in the supernatant was determined using an ELISA kit.