phenol choloroform isoamyl alcohol  (Thermo Fisher)


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    Structured Review

    Thermo Fisher phenol choloroform isoamyl alcohol
    Phenol Choloroform Isoamyl Alcohol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenol choloroform isoamyl alcohol/product/Thermo Fisher
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    phenol choloroform isoamyl alcohol - by Bioz Stars, 2020-09
    88/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: RNA-recognition motif in Matrin-3 mediates neurodegeneration through interaction with hnRNPM
    Article Snippet: .. 20ug proteinase K and 1% final concentration SDS were added to each sample including “Input” followed by incubation at 65 °C for 1 h. The beads were purified using MagnaRack™ (Invitrogen CS15000) followed by RNA extraction using phenol-choloroform-isoamyl alcohol. cDNA synthesis was performed using the Applied Biosystems High capacity cDNA Reverse Transcription kit (4368814) and was subsequently run using the Bio-Rad iQ™Supermix (170-8862) on a 96-well plate (Applied Biosystems, #4306737) on Applied Biosystems StepOnePlus Real-Time PCR system. .. Ct values from FLAG-immunoprecipitation and V5-immunoprecipitation from each group were normalized to their respective “Input” Ct value (ΔCt) and normalized again to “-ve Ab control” (ΔΔCt).

    Concentration Assay:

    Article Title: RNA-recognition motif in Matrin-3 mediates neurodegeneration through interaction with hnRNPM
    Article Snippet: .. 20ug proteinase K and 1% final concentration SDS were added to each sample including “Input” followed by incubation at 65 °C for 1 h. The beads were purified using MagnaRack™ (Invitrogen CS15000) followed by RNA extraction using phenol-choloroform-isoamyl alcohol. cDNA synthesis was performed using the Applied Biosystems High capacity cDNA Reverse Transcription kit (4368814) and was subsequently run using the Bio-Rad iQ™Supermix (170-8862) on a 96-well plate (Applied Biosystems, #4306737) on Applied Biosystems StepOnePlus Real-Time PCR system. .. Ct values from FLAG-immunoprecipitation and V5-immunoprecipitation from each group were normalized to their respective “Input” Ct value (ΔCt) and normalized again to “-ve Ab control” (ΔΔCt).

    Incubation:

    Article Title: RNA-recognition motif in Matrin-3 mediates neurodegeneration through interaction with hnRNPM
    Article Snippet: .. 20ug proteinase K and 1% final concentration SDS were added to each sample including “Input” followed by incubation at 65 °C for 1 h. The beads were purified using MagnaRack™ (Invitrogen CS15000) followed by RNA extraction using phenol-choloroform-isoamyl alcohol. cDNA synthesis was performed using the Applied Biosystems High capacity cDNA Reverse Transcription kit (4368814) and was subsequently run using the Bio-Rad iQ™Supermix (170-8862) on a 96-well plate (Applied Biosystems, #4306737) on Applied Biosystems StepOnePlus Real-Time PCR system. .. Ct values from FLAG-immunoprecipitation and V5-immunoprecipitation from each group were normalized to their respective “Input” Ct value (ΔCt) and normalized again to “-ve Ab control” (ΔΔCt).

    RNA Extraction:

    Article Title: RNA-recognition motif in Matrin-3 mediates neurodegeneration through interaction with hnRNPM
    Article Snippet: .. 20ug proteinase K and 1% final concentration SDS were added to each sample including “Input” followed by incubation at 65 °C for 1 h. The beads were purified using MagnaRack™ (Invitrogen CS15000) followed by RNA extraction using phenol-choloroform-isoamyl alcohol. cDNA synthesis was performed using the Applied Biosystems High capacity cDNA Reverse Transcription kit (4368814) and was subsequently run using the Bio-Rad iQ™Supermix (170-8862) on a 96-well plate (Applied Biosystems, #4306737) on Applied Biosystems StepOnePlus Real-Time PCR system. .. Ct values from FLAG-immunoprecipitation and V5-immunoprecipitation from each group were normalized to their respective “Input” Ct value (ΔCt) and normalized again to “-ve Ab control” (ΔΔCt).

    Purification:

    Article Title: RNA-recognition motif in Matrin-3 mediates neurodegeneration through interaction with hnRNPM
    Article Snippet: .. 20ug proteinase K and 1% final concentration SDS were added to each sample including “Input” followed by incubation at 65 °C for 1 h. The beads were purified using MagnaRack™ (Invitrogen CS15000) followed by RNA extraction using phenol-choloroform-isoamyl alcohol. cDNA synthesis was performed using the Applied Biosystems High capacity cDNA Reverse Transcription kit (4368814) and was subsequently run using the Bio-Rad iQ™Supermix (170-8862) on a 96-well plate (Applied Biosystems, #4306737) on Applied Biosystems StepOnePlus Real-Time PCR system. .. Ct values from FLAG-immunoprecipitation and V5-immunoprecipitation from each group were normalized to their respective “Input” Ct value (ΔCt) and normalized again to “-ve Ab control” (ΔΔCt).

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