pgp 9 5 antisera  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    Protein Gene Product 9 5 PGP9 5
    Description:

    Catalog Number:
    rb-9202-pcs
    Price:
    None
    Buy from Supplier


    Structured Review

    Thermo Fisher pgp 9 5 antisera
    Characterization of neural cells in mouse striatal cultures (A-G). (A) Phase contrast photomicrograph of striatal cells at 5-7 days  in vitro . A majority of the cells are neurons; scale bar = 20 μm. (B) Astrocytes were characterized by large nuclei with multiple nucleoli and dispersed heterochromatin (Hoechst 33342 blue immunofluorescence) and glial fibrillary acidic (GFAP) immunofluorescence (Cy3 red fluorochrome); scale bar = 25 μm. (C-E) Subpopulations of striatal neurons possessed μ (C), κ (E), and to a lesser extent δ (not shown), opioid receptor immunoreactivity. D    E show phase contrast and κ opioid receptor immunofluorescent images of the same cells; scale bar = 20 μm. (F-G) Triple-label-identification of neurons, astrocytes and microglia in striatal cultures. Neurons were identified using anti-PGP 9.5 indirect immunofluorescence (Alexa 488, green product), while astrocytes were labeled using anti-GFAP indirect immunofluorescence (Alexa 546, red product) and microglia were detected using IB 4  biotinylated isolectin-conjugated (via avidin) to Alexa 350 (blue fluorescent product) (arrow) (F). A majority of the cells were neurons; microglia comprised 0.7% of the total cells. (G) A phase-contrast photomicrograph of the same cells as in Fig. 1F, some of which were non-viable/degenerating (hatched arrows); PGP 9.5 immunoreactivity is retained by many degenerating neurons; scale bar = 20 μm.

    https://www.bioz.com/result/pgp 9 5 antisera/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pgp 9 5 antisera - by Bioz Stars, 2020-07
    94/100 stars

    Images

    1) Product Images from "Synergistic Neurotoxicity of Opioids and Human Immunodeficiency Virus-1 Tat Protein in Striatal Neurons In Vitro"

    Article Title: Synergistic Neurotoxicity of Opioids and Human Immunodeficiency Virus-1 Tat Protein in Striatal Neurons In Vitro

    Journal: Neuroscience

    doi:

    Characterization of neural cells in mouse striatal cultures (A-G). (A) Phase contrast photomicrograph of striatal cells at 5-7 days  in vitro . A majority of the cells are neurons; scale bar = 20 μm. (B) Astrocytes were characterized by large nuclei with multiple nucleoli and dispersed heterochromatin (Hoechst 33342 blue immunofluorescence) and glial fibrillary acidic (GFAP) immunofluorescence (Cy3 red fluorochrome); scale bar = 25 μm. (C-E) Subpopulations of striatal neurons possessed μ (C), κ (E), and to a lesser extent δ (not shown), opioid receptor immunoreactivity. D    E show phase contrast and κ opioid receptor immunofluorescent images of the same cells; scale bar = 20 μm. (F-G) Triple-label-identification of neurons, astrocytes and microglia in striatal cultures. Neurons were identified using anti-PGP 9.5 indirect immunofluorescence (Alexa 488, green product), while astrocytes were labeled using anti-GFAP indirect immunofluorescence (Alexa 546, red product) and microglia were detected using IB 4  biotinylated isolectin-conjugated (via avidin) to Alexa 350 (blue fluorescent product) (arrow) (F). A majority of the cells were neurons; microglia comprised 0.7% of the total cells. (G) A phase-contrast photomicrograph of the same cells as in Fig. 1F, some of which were non-viable/degenerating (hatched arrows); PGP 9.5 immunoreactivity is retained by many degenerating neurons; scale bar = 20 μm.
    Figure Legend Snippet: Characterization of neural cells in mouse striatal cultures (A-G). (A) Phase contrast photomicrograph of striatal cells at 5-7 days in vitro . A majority of the cells are neurons; scale bar = 20 μm. (B) Astrocytes were characterized by large nuclei with multiple nucleoli and dispersed heterochromatin (Hoechst 33342 blue immunofluorescence) and glial fibrillary acidic (GFAP) immunofluorescence (Cy3 red fluorochrome); scale bar = 25 μm. (C-E) Subpopulations of striatal neurons possessed μ (C), κ (E), and to a lesser extent δ (not shown), opioid receptor immunoreactivity. D E show phase contrast and κ opioid receptor immunofluorescent images of the same cells; scale bar = 20 μm. (F-G) Triple-label-identification of neurons, astrocytes and microglia in striatal cultures. Neurons were identified using anti-PGP 9.5 indirect immunofluorescence (Alexa 488, green product), while astrocytes were labeled using anti-GFAP indirect immunofluorescence (Alexa 546, red product) and microglia were detected using IB 4 biotinylated isolectin-conjugated (via avidin) to Alexa 350 (blue fluorescent product) (arrow) (F). A majority of the cells were neurons; microglia comprised 0.7% of the total cells. (G) A phase-contrast photomicrograph of the same cells as in Fig. 1F, some of which were non-viable/degenerating (hatched arrows); PGP 9.5 immunoreactivity is retained by many degenerating neurons; scale bar = 20 μm.

    Techniques Used: In Vitro, Immunofluorescence, Labeling, Avidin-Biotin Assay

    Related Articles

    Immunohistochemistry:

    Article Title: The deep fascia and retinacula of the equine forelimb – structure and innervation
    Article Snippet: .. For immunohistochemical staining of general nervous tissue markers the following primary antibodies were used: anti‐S‐100 protein (DAKO, 1 : 5000) and anti‐protein gene product 9.5 (Thermo Scientific, 1 : 3000). .. Sections were deparaffinized, rehydrated and peroxidase activity was removed with 0.6% H2 O2 in methanol.

    Article Title: Influence of the neural microenvironment on prostate cancer, et al. Influence of the neural microenvironment on prostate cancer
    Article Snippet: .. Immunohistochemistry was performed using standardized procedures with the following antibodies: PGP9.5 (Protein Gene Product 9.5) (incubation of primary antibody, AB1, clone 10A1, cat #Ms‐1760 NeoMarkers/Thermo Scientific) at 1:30 overnight at 4°C and then 3 h at room temperature); and Ki‐67 (incubation of primary antibody (Ki‐67 rbMab, cat #RM‐9106) at 1:400 for 30 min at room temperature). .. 2.15 Imaging and image analysis Hot spot areas of expression of biomarkers within the PCa and non neoplastic tissues were imaged using the Nuance image deconvolution system (Four 200× images per area).

    Labeling:

    Article Title: Analyzing Somatosensory Axon Projections with the Sensory Neuron-Specific Advillin Gene
    Article Snippet: .. Sections were then incubated with anti-protein gene product 9.5 (PGP9.5) antibody (1:1000; UltraClone, Isle of Wight, UK) at 4°C overnight and detected with Alexa488 -labeled anti-rabbit IgG (1:400; Invitrogen). .. For immunostaining combined with fluorescent red AP staining, the sections were fixed in 4% PFA/PBS solution and heat-inactivated in PBS as described above.

    Incubation:

    Article Title: Schwann Cell-Derived CCL2 Promotes the Perineural Invasion of Cervical Cancer
    Article Snippet: .. Immunofluorescence Assay For identification of cancer cells and DRGs, the cells were fixed with 4% paraformaldehyde for 1 h and blocked with 0.03% Triton X-100 containing 5% calf serum in PBS for 1 h at room temperature and then incubated with the antibody pan-cytokeratin (CST-4544, Cell Signaling Technology), PGP9.5 (PA5-16825, ThermoFisher), Neurofilament-heavy (PA3-16753, ThermoFisher), and Collagen IV (ab6586, Abcam) at 4°C overnight. .. Then, the cells were incubated with a secondary antibody conjugated with Alexa Fluor®488 (A-11006, ThermoFisher) and Alexa Fluor® 594 (A-11007, ThermoFisher).

    Article Title: Influence of the neural microenvironment on prostate cancer, et al. Influence of the neural microenvironment on prostate cancer
    Article Snippet: .. Immunohistochemistry was performed using standardized procedures with the following antibodies: PGP9.5 (Protein Gene Product 9.5) (incubation of primary antibody, AB1, clone 10A1, cat #Ms‐1760 NeoMarkers/Thermo Scientific) at 1:30 overnight at 4°C and then 3 h at room temperature); and Ki‐67 (incubation of primary antibody (Ki‐67 rbMab, cat #RM‐9106) at 1:400 for 30 min at room temperature). .. 2.15 Imaging and image analysis Hot spot areas of expression of biomarkers within the PCa and non neoplastic tissues were imaged using the Nuance image deconvolution system (Four 200× images per area).

    Article Title: Analyzing Somatosensory Axon Projections with the Sensory Neuron-Specific Advillin Gene
    Article Snippet: .. Sections were then incubated with anti-protein gene product 9.5 (PGP9.5) antibody (1:1000; UltraClone, Isle of Wight, UK) at 4°C overnight and detected with Alexa488 -labeled anti-rabbit IgG (1:400; Invitrogen). .. For immunostaining combined with fluorescent red AP staining, the sections were fixed in 4% PFA/PBS solution and heat-inactivated in PBS as described above.

    other:

    Article Title: Heterogeneous ventricular sympathetic innervation, altered ?-adrenergic receptor expression, and rhythm instability in mice lacking the p75 neurotrophin receptor
    Article Snippet: TH-specific antibody was from Chemicon; protein gene product 9.5 (PGP9.5)-specific antibody was from Accurate Chemicals; β1 AR-specific antibody was from Affinity Bioreagents; horseradish peroxidase-conjugated rabbit IgG-specific antibody was from Pierce; and Alexa fluor488-conjugated rabbit IgG-specific antibody was from Molecular Probes.

    Mass Spectrometry:

    Article Title: Influence of the neural microenvironment on prostate cancer, et al. Influence of the neural microenvironment on prostate cancer
    Article Snippet: .. Immunohistochemistry was performed using standardized procedures with the following antibodies: PGP9.5 (Protein Gene Product 9.5) (incubation of primary antibody, AB1, clone 10A1, cat #Ms‐1760 NeoMarkers/Thermo Scientific) at 1:30 overnight at 4°C and then 3 h at room temperature); and Ki‐67 (incubation of primary antibody (Ki‐67 rbMab, cat #RM‐9106) at 1:400 for 30 min at room temperature). .. 2.15 Imaging and image analysis Hot spot areas of expression of biomarkers within the PCa and non neoplastic tissues were imaged using the Nuance image deconvolution system (Four 200× images per area).

    Staining:

    Article Title: The deep fascia and retinacula of the equine forelimb – structure and innervation
    Article Snippet: .. For immunohistochemical staining of general nervous tissue markers the following primary antibodies were used: anti‐S‐100 protein (DAKO, 1 : 5000) and anti‐protein gene product 9.5 (Thermo Scientific, 1 : 3000). .. Sections were deparaffinized, rehydrated and peroxidase activity was removed with 0.6% H2 O2 in methanol.

    Article Title: Schwann Cell-Derived CCL2 Promotes the Perineural Invasion of Cervical Cancer
    Article Snippet: .. Cancer cells colonized and spread along the nerve fibers, which were validated by H & E staining of sciatic nerves and double immunofluorescent staining of PGP9.5 and pan-CK ( ). ..

    Immunofluorescence:

    Article Title: Schwann Cell-Derived CCL2 Promotes the Perineural Invasion of Cervical Cancer
    Article Snippet: .. Immunofluorescence Assay For identification of cancer cells and DRGs, the cells were fixed with 4% paraformaldehyde for 1 h and blocked with 0.03% Triton X-100 containing 5% calf serum in PBS for 1 h at room temperature and then incubated with the antibody pan-cytokeratin (CST-4544, Cell Signaling Technology), PGP9.5 (PA5-16825, ThermoFisher), Neurofilament-heavy (PA3-16753, ThermoFisher), and Collagen IV (ab6586, Abcam) at 4°C overnight. .. Then, the cells were incubated with a secondary antibody conjugated with Alexa Fluor®488 (A-11006, ThermoFisher) and Alexa Fluor® 594 (A-11007, ThermoFisher).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Thermo Fisher primary rabbit polyclonal antisera against pgp9 5
    Primary Rabbit Polyclonal Antisera Against Pgp9 5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary rabbit polyclonal antisera against pgp9 5/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary rabbit polyclonal antisera against pgp9 5 - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results