Structured Review

Thermo Fisher pgex6p 2
Pgex6p 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgex6p 2/product/Thermo Fisher
Average 80 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pgex6p 2 - by Bioz Stars, 2020-04
80/100 stars

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Related Articles

Construct:

Article Title: Ubiquitin carboxyl‐terminal hydrolase L1 promotes hypoxia‐inducible factor 1‐dependent tumor cell malignancy in spheroid models. Ubiquitin carboxyl‐terminal hydrolase L1 promotes hypoxia‐inducible factor 1‐dependent tumor cell malignancy in spheroid models
Article Snippet: 2.1 Plasmids and purification of recombinant protein To construct pGEX6p‐2/UCHL1, DNA encoding human UCHL1 gene was digested between EcoRV and XhoI in pcDNA4/UCHL1. .. This DNA fragment was then inserted between the SmaI and XhoI sites of PGEX6p‐2 (Invitrogen).

Purification:

Article Title: Ubiquitin carboxyl‐terminal hydrolase L1 promotes hypoxia‐inducible factor 1‐dependent tumor cell malignancy in spheroid models. Ubiquitin carboxyl‐terminal hydrolase L1 promotes hypoxia‐inducible factor 1‐dependent tumor cell malignancy in spheroid models
Article Snippet: Paragraph title: Plasmids and purification of recombinant protein ... This DNA fragment was then inserted between the SmaI and XhoI sites of PGEX6p‐2 (Invitrogen).

Sonication:

Article Title: Ubiquitin carboxyl‐terminal hydrolase L1 promotes hypoxia‐inducible factor 1‐dependent tumor cell malignancy in spheroid models. Ubiquitin carboxyl‐terminal hydrolase L1 promotes hypoxia‐inducible factor 1‐dependent tumor cell malignancy in spheroid models
Article Snippet: This DNA fragment was then inserted between the SmaI and XhoI sites of PGEX6p‐2 (Invitrogen). .. E coli DH5α was treated with sonication and dissolved in lysis buffer (50 mmol/L Tris‐HCl [pH 8.0], 0.1 mol/L NaCl, 1 mmol/L EDTA, 1 mmol/L DTT, 1% Triton X‐100) The fusion protein GST/UCHL1 was first purified with glutathione‐Sepharose 4B beads (GE Healthcare UK) and eluted with 20 mmol/L of glutathione (GSH; pH 8.5).

Lysis:

Article Title: Ubiquitin carboxyl‐terminal hydrolase L1 promotes hypoxia‐inducible factor 1‐dependent tumor cell malignancy in spheroid models. Ubiquitin carboxyl‐terminal hydrolase L1 promotes hypoxia‐inducible factor 1‐dependent tumor cell malignancy in spheroid models
Article Snippet: This DNA fragment was then inserted between the SmaI and XhoI sites of PGEX6p‐2 (Invitrogen). .. E coli DH5α was treated with sonication and dissolved in lysis buffer (50 mmol/L Tris‐HCl [pH 8.0], 0.1 mol/L NaCl, 1 mmol/L EDTA, 1 mmol/L DTT, 1% Triton X‐100) The fusion protein GST/UCHL1 was first purified with glutathione‐Sepharose 4B beads (GE Healthcare UK) and eluted with 20 mmol/L of glutathione (GSH; pH 8.5).

Recombinant:

Article Title: Ubiquitin carboxyl‐terminal hydrolase L1 promotes hypoxia‐inducible factor 1‐dependent tumor cell malignancy in spheroid models. Ubiquitin carboxyl‐terminal hydrolase L1 promotes hypoxia‐inducible factor 1‐dependent tumor cell malignancy in spheroid models
Article Snippet: Paragraph title: Plasmids and purification of recombinant protein ... This DNA fragment was then inserted between the SmaI and XhoI sites of PGEX6p‐2 (Invitrogen).

Plasmid Preparation:

Article Title: Ubiquitin carboxyl‐terminal hydrolase L1 promotes hypoxia‐inducible factor 1‐dependent tumor cell malignancy in spheroid models. Ubiquitin carboxyl‐terminal hydrolase L1 promotes hypoxia‐inducible factor 1‐dependent tumor cell malignancy in spheroid models
Article Snippet: This DNA fragment was then inserted between the SmaI and XhoI sites of PGEX6p‐2 (Invitrogen). .. E coli DH5α harboring pGEX6p‐2/UCHL1 plasmid was induced with isopropyl β‐D‐1‐thiogalactopyranoside.

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    Thermo Fisher pgex6p construct
    Pgex6p Construct, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgex6p construct/product/Thermo Fisher
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pgex6p construct - by Bioz Stars, 2020-04
    88/100 stars
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