Journal: Poultry Science

Article Title: Resveratrol protects against triclosan–induced impairment of colonic barrier function in chickens

doi: 10.1016/j.psj.2026.106946

Figure Lengend Snippet: Effects of RSV on mitochondrial function–related indicators exposed to TCS. (A, B) Protein expression and quantitative analysis of SIRT1, PGC–1α, NRF1, and mRNA expression levels (C) mRNA expression levels of SIRT1, PGC–1α, NRF1, and TFAM (D) mRNA expression levels of MFN2, OPA1, MFN1, DRP1, FIS1, and MFF. Data were analyzed using one–way ANOVA. Inter–group differences are indicated by different letters ( P < 0.05). Groups with the same letter were considered not to have significant differences (mean ± SD, n = 6).

Article Snippet: PGC-1α , 1:600 , WANLEIBIO.

Techniques: Expressing

Journal: Clinical and Translational Medicine

Article Title: α7nAChR agonist GTS‐21 ameliorates sepsis‐induced acute kidney injury via MEF2/PGC‐1α/HO‐1 axis in mice

doi: 10.1002/ctm2.70726

Figure Lengend Snippet: α7nAChR knockout reverses the protective effects of GTS‐21 against LPS‐induced injury in HK‐2 cells. (A) Validation of α7nAChR deficiency in HK‐2 cells. (B) Representative TEM images of HK‐2 cells. Scale bar = 2 µm. (C–E) Effects of α7nAChR on MMP, mito‐ROS levels and apoptosis in HK‐2 cells. (F–J) Representative Western blot images and quantitative analysis of CytC, MEF2, HO‐1 and PGC‐1α in HK‐2 cells. (K–M) mRNA expression levels of MEF2, HO‐1 and PGC‐1α in HK‐2 cells. Data are presented as mean ± SD. * p < .05, ** p < .01.

Article Snippet: In addition, the PGC‐1α inhibitor SR‐18292 (MCE, HY‐101491) and the HO‐1 inhibitor tin protoporphyrin IX (MCE, HY‐101194) were dissolved in DMSO and administered via intraperitoneal injection at a dose of 5 mg/kg at 12 h post‐CLP.

Techniques: Knock-Out, Biomarker Discovery, Western Blot, Expressing

Journal: Clinical and Translational Medicine

Article Title: α7nAChR agonist GTS‐21 ameliorates sepsis‐induced acute kidney injury via MEF2/PGC‐1α/HO‐1 axis in mice

doi: 10.1002/ctm2.70726

Figure Lengend Snippet: α7nAChR knockout reverses the protective effects of GTS‐21 against LPS‐induced injury in mRTECs. (A) Validation of α7nAChR deficiency in mRTECs. (B) Representative TEM images of mRTECs. Scale bar = 2 µm. (C–E) Effects of α7nAChR deficiency on MMP, mito‐ROS levels and apoptosis in mRTECs. (F–J) Representative Western blot images and quantitative analysis of CytC, MEF2, HO‐1 and PGC‐1α in mRTECs. (K–M) mRNA expression levels of MEF2, HO‐1 and PGC‐1α in mRTECs. Data are presented as mean ± SD. * p < .05, ** p < .01.

Article Snippet: In addition, the PGC‐1α inhibitor SR‐18292 (MCE, HY‐101491) and the HO‐1 inhibitor tin protoporphyrin IX (MCE, HY‐101194) were dissolved in DMSO and administered via intraperitoneal injection at a dose of 5 mg/kg at 12 h post‐CLP.

Techniques: Knock-Out, Biomarker Discovery, Western Blot, Expressing

Journal: Clinical and Translational Medicine

Article Title: α7nAChR agonist GTS‐21 ameliorates sepsis‐induced acute kidney injury via MEF2/PGC‐1α/HO‐1 axis in mice

doi: 10.1002/ctm2.70726

Figure Lengend Snippet: MEF2 contributes to GTS‐21‐mediated upregulation of PGC‐1α and HO‐1 in HK‐2 cells. (A) Representative TEM images of HK‐2 cells. Scale bar = 2 µm. (B–D) Effects of MEF2 deficiency on MMP, mito‐ROS levels and apoptosis in HK‐2 cells after LPS stimulation. (E–I) Representative Western blot images and quantitative analysis of Cyt c, MEF2, HO‐1 and PGC‐1α in MEF2‐deficient HK‐2 cells following LPS stimulation. (J–L) mRNA expression levels of MEF2, HO‐1 and PGC‐1α in MEF2‐deficient HK‐2 cells following LPS stimulation. Data are presented as mean ± SD. * p < .05, ** p < .01.

Article Snippet: In addition, the PGC‐1α inhibitor SR‐18292 (MCE, HY‐101491) and the HO‐1 inhibitor tin protoporphyrin IX (MCE, HY‐101194) were dissolved in DMSO and administered via intraperitoneal injection at a dose of 5 mg/kg at 12 h post‐CLP.

Techniques: Western Blot, Expressing

Journal: Clinical and Translational Medicine

Article Title: α7nAChR agonist GTS‐21 ameliorates sepsis‐induced acute kidney injury via MEF2/PGC‐1α/HO‐1 axis in mice

doi: 10.1002/ctm2.70726

Figure Lengend Snippet: MEF2 contributes to GTS‐21‐mediated upregulation of PGC‐1α and HO‐1 in mRTECs. (A) Representative TEM images of mRTECs. Scale bar = 2 µm. (B–D) Effects of MEF2 deficiency on MMP, mito‐ROS levels and apoptosis in mRTECs following LPS stimulation. (E–I) Representative Western blot images and quantitative analysis of Cyt c, MEF2, HO‐1 and PGC‐1α in MEF2‐deficient mRTECs following LPS stimulation. (J–L) mRNA expression levels of MEF2, HO‐1 and PGC‐1α in MEF2‐deficient mRTECs following LPS stimulation. Data are presented as mean ± SD. * p < .05, ** p < .01.

Article Snippet: In addition, the PGC‐1α inhibitor SR‐18292 (MCE, HY‐101491) and the HO‐1 inhibitor tin protoporphyrin IX (MCE, HY‐101194) were dissolved in DMSO and administered via intraperitoneal injection at a dose of 5 mg/kg at 12 h post‐CLP.

Techniques: Western Blot, Expressing

Journal: Clinical and Translational Medicine

Article Title: α7nAChR agonist GTS‐21 ameliorates sepsis‐induced acute kidney injury via MEF2/PGC‐1α/HO‐1 axis in mice

doi: 10.1002/ctm2.70726

Figure Lengend Snippet: GTS‐21 ameliorates mitochondrial damage and promotes mitochondrial biogenesis in CLP‐induced mice. (A)Validation of α7nAChR deficiency in mouse kidney tissues. (B and C) Effects of GTS‐21 on survival in CLP‐induced mice. ( n = 6) (D) Effects of α7nAChR on serum creatinine levels in CLP‐induced mice. (E) Representative H&E‐stained images of kidney tissues from CLP‐induced mice. Scale bar = 50 µm. (F) Representative TEM images of mitochondrial ultrastructure in kidney tissues from different experimental groups, including Sham, CLP and CLP + GTS‐21 mice. Scale bar = 2 µm. (G‐J) Effects of α7nAChR on serum levels of NGAL, IL‐1β, TNF‐α and IL‐6 in CLP‐induced mice. (K–M) Effects of α7nAChR on MMP, mito‐ROS levels and apoptosis in CLP‐induced mice. (N–R) Representative Western blot images and quantitative analysis of CytC, MEF2, HO‐1, and PGC‐1α in CLP‐induced mice. (T‐U) mRNA expression levels of MEF2, HO‐1 and PGC‐1α in CLP‐induced mice. Data are presented as mean ± SD. * p < .05, ** p < .01. ( n = 3mice per group).

Article Snippet: In addition, the PGC‐1α inhibitor SR‐18292 (MCE, HY‐101491) and the HO‐1 inhibitor tin protoporphyrin IX (MCE, HY‐101194) were dissolved in DMSO and administered via intraperitoneal injection at a dose of 5 mg/kg at 12 h post‐CLP.

Techniques: Biomarker Discovery, Staining, Western Blot, Expressing

Journal: Clinical and Translational Medicine

Article Title: α7nAChR agonist GTS‐21 ameliorates sepsis‐induced acute kidney injury via MEF2/PGC‐1α/HO‐1 axis in mice

doi: 10.1002/ctm2.70726

Figure Lengend Snippet: GTS‐21 ameliorates CLP‐induced injury in mice in a MEF2/PGC‐1α/HO‐1‐dependent manner. (A) Effects of MEF2 deficiency or inhibition of PGC‐1α and HO‐1 on survival in CLP‐induced mice. (B) Effects of MEF2 deficiency or inhibition of PGC‐1α and HO‐1 on serum creatinine levels in CLP‐induced mice. ( n = 6) (C) Representative H&E‐stained images of kidney tissues from CLP‐induced mice. Scale bar = 50 µm. (D) Representative TEM images of kidney tissues from CLP‐induced mice. Scale bar = 2 µm. (E–H) Effects of MEF2 deficiency or inhibition of PGC‐1α and HO‐1 on serum levels of NGAL, IL‐1β, TNF‐α and IL‐6 in CLP‐induced mice. (I–K) Effects of MEF2 deficiency or inhibition of PGC‐1α and HO‐1 on MMP, mitochondrial ROS levels and apoptosis in CLP‐induced mice. (L–O) Representative Western blot images and quantitative analysis of MEF2, HO‐1 and PGC‐1α in CLP‐induced mice. (P–R) mRNA expression levels of MEF2, HO‐1 and PGC‐1α in CLP‐induced mice. Data are presented as mean ± SD. *p < .05, **p < .01. ( n = 3mice per group).

Article Snippet: In addition, the PGC‐1α inhibitor SR‐18292 (MCE, HY‐101491) and the HO‐1 inhibitor tin protoporphyrin IX (MCE, HY‐101194) were dissolved in DMSO and administered via intraperitoneal injection at a dose of 5 mg/kg at 12 h post‐CLP.

Techniques: Inhibition, Staining, Western Blot, Expressing