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Agilent technologies pfuturbo cx hotstart dna polymerase
Pfuturbo Cx Hotstart Dna Polymerase, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pfuturbo cx hotstart dna polymerase/product/Agilent technologies
Average 90 stars, based on 53 article reviews
Price from $9.99 to $1999.99
pfuturbo cx hotstart dna polymerase - by Bioz Stars, 2020-07
90/100 stars

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Polymerase Chain Reaction:

Article Title: Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency
Article Snippet: .. We generated RRBS libraries by PCR amplification with PfUTurbo Cx hotstart DNA polymerase (Agilent) and indexed Illumina primers using the following PCR conditions: 95 °C for 2 min, 12 cycles (95 °C for 30 s, 65 °C for 30 s, 72 °C for 45 s) and 72 °C for 7 min. .. The libraries were purified with AMPure magnetic beads (Beckman Coulter) and paired end sequenced (2 × 75 bp) in multiplex on an Illumina HiSeq2000, to generate ∼35 million pairs of reads per sample.

Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
Article Snippet: .. In order to determine the minimum number of PCR cycles required for final library amplification, 50 μl PCR reactions containing 3 μl bisulfite-converted DNA, 5 μl 10× PfuTurbo Cx hotstart DNA polymerase buffer, 0.5 μl 100 mM dNTP (25 mM each dNTP) (Agilent, 200415), 0.5 μl Illumina TruSeq PCR primers (25 μM each primer) and 1 μl PfuTurbo Cx hotstart DNA polymerase (Agilent, 600412) were prepared. .. Reactions were then split equally into four separate tubes and thermocycled using the following conditions: denature at 95 °C for 2 min followed by X cycles of 95 °C for 30 s, 65 °C for 30 s, 72 °C for 45 s (where X number of cycles = 11, 13, 15 and 17), followed by a final extension at 72 °C for 7 min. PCR products were purified using 1.2× volumes of Ampure XP and analysed on an Agilent Bioanalyzer using a High Sensitivity DNA kit (Agilent, 5067-4626).

Article Title: Genome-Wide Methylation Profiling in Canine Mammary Tumor Reveals miRNA Candidates Associated with Human Breast Cancer
Article Snippet: .. The final libraries were generated by PCR amplification using PfuTurbo Cx Hotstart DNA polymerase (Agilent technologies, Santa Clara, CA, USA). .. RRBS libraries were analyzed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Epstein-Barr virus BORF2 inhibits cellular APOBEC3B to preserve viral genome integrity
Article Snippet: .. Genomic DNA was harvested from cells 3 days after induction with PMA/NaB and portions of the EBV genome were PCR amplified using PfuTurbo Cx Hotstart DNA Polymerase (Agilent 600410). .. PCR products correspond to regions near the genes BRRF2 (95085-95584), LMP1 (167979-168514), BHRF1 (42090-42595), and BcLF1 (124082-124598).

Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
Article Snippet: .. In order to determine the minimum number of PCR cycles required for final library amplification, 50 μl PCR reactions containing 3 μl bisulfite-converted DNA, 5 μl 10× PfuTurbo Cx hotstart DNA polymerase buffer, 0.5 μl 100 mM dNTP (25 mM each dNTP) (Agilent, 200415), 0.5 μl Illumina TruSeq PCR primers (25 μM each primer) and 1 μl PfuTurbo Cx hotstart DNA polymerase (Agilent, 600412) were prepared. .. Reactions were then split equally into four separate tubes and thermocycled using the following conditions: denature at 95 °C for 2 min followed by X cycles of 95 °C for 30 s, 65 °C for 30 s, 72 °C for 45 s (where X number of cycles = 11, 13, 15 and 17), followed by a final extension at 72 °C for 7 min. PCR products were purified using 1.2× volumes of Ampure XP and analysed on an Agilent Bioanalyzer using a High Sensitivity DNA kit (Agilent, 5067-4626).

Immunoprecipitation:

Article Title: Candidate genes responsible for early key events of phenobarbital-promoted mouse hepatocellular tumorigenesis based on differentiation of regulating genes between wild type mice and humanized chimeric mice genes responsible for early key events of phenobarbital-promoted mouse hepatocellular tumorigenesis based on differentiation of regulating genes
Article Snippet: .. Immunoprecipitated DNA and input DNA were purified as described above, and amplified using Primer-3 (see ESI Table S1 ) for 17 cycles in a reaction containing 400 μM dATP, 400 μM dCTP, 400 μM dGTP, 320 μM dTTP, 80 μM dUTP, PfuTurbo Cx Hotstart DNA Polymerase (Agilent Technologies) and 10× reaction buffer. .. Amplified DNA was purified as described above.

Generated:

Article Title: Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency
Article Snippet: .. We generated RRBS libraries by PCR amplification with PfUTurbo Cx hotstart DNA polymerase (Agilent) and indexed Illumina primers using the following PCR conditions: 95 °C for 2 min, 12 cycles (95 °C for 30 s, 65 °C for 30 s, 72 °C for 45 s) and 72 °C for 7 min. .. The libraries were purified with AMPure magnetic beads (Beckman Coulter) and paired end sequenced (2 × 75 bp) in multiplex on an Illumina HiSeq2000, to generate ∼35 million pairs of reads per sample.

Article Title: Genome-Wide Methylation Profiling in Canine Mammary Tumor Reveals miRNA Candidates Associated with Human Breast Cancer
Article Snippet: .. The final libraries were generated by PCR amplification using PfuTurbo Cx Hotstart DNA polymerase (Agilent technologies, Santa Clara, CA, USA). .. RRBS libraries were analyzed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).

Amplification:

Article Title: Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency
Article Snippet: .. We generated RRBS libraries by PCR amplification with PfUTurbo Cx hotstart DNA polymerase (Agilent) and indexed Illumina primers using the following PCR conditions: 95 °C for 2 min, 12 cycles (95 °C for 30 s, 65 °C for 30 s, 72 °C for 45 s) and 72 °C for 7 min. .. The libraries were purified with AMPure magnetic beads (Beckman Coulter) and paired end sequenced (2 × 75 bp) in multiplex on an Illumina HiSeq2000, to generate ∼35 million pairs of reads per sample.

Article Title: Candidate genes responsible for early key events of phenobarbital-promoted mouse hepatocellular tumorigenesis based on differentiation of regulating genes between wild type mice and humanized chimeric mice genes responsible for early key events of phenobarbital-promoted mouse hepatocellular tumorigenesis based on differentiation of regulating genes
Article Snippet: .. Immunoprecipitated DNA and input DNA were purified as described above, and amplified using Primer-3 (see ESI Table S1 ) for 17 cycles in a reaction containing 400 μM dATP, 400 μM dCTP, 400 μM dGTP, 320 μM dTTP, 80 μM dUTP, PfuTurbo Cx Hotstart DNA Polymerase (Agilent Technologies) and 10× reaction buffer. .. Amplified DNA was purified as described above.

Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
Article Snippet: .. In order to determine the minimum number of PCR cycles required for final library amplification, 50 μl PCR reactions containing 3 μl bisulfite-converted DNA, 5 μl 10× PfuTurbo Cx hotstart DNA polymerase buffer, 0.5 μl 100 mM dNTP (25 mM each dNTP) (Agilent, 200415), 0.5 μl Illumina TruSeq PCR primers (25 μM each primer) and 1 μl PfuTurbo Cx hotstart DNA polymerase (Agilent, 600412) were prepared. .. Reactions were then split equally into four separate tubes and thermocycled using the following conditions: denature at 95 °C for 2 min followed by X cycles of 95 °C for 30 s, 65 °C for 30 s, 72 °C for 45 s (where X number of cycles = 11, 13, 15 and 17), followed by a final extension at 72 °C for 7 min. PCR products were purified using 1.2× volumes of Ampure XP and analysed on an Agilent Bioanalyzer using a High Sensitivity DNA kit (Agilent, 5067-4626).

Article Title: Genome-Wide Methylation Profiling in Canine Mammary Tumor Reveals miRNA Candidates Associated with Human Breast Cancer
Article Snippet: .. The final libraries were generated by PCR amplification using PfuTurbo Cx Hotstart DNA polymerase (Agilent technologies, Santa Clara, CA, USA). .. RRBS libraries were analyzed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Epstein-Barr virus BORF2 inhibits cellular APOBEC3B to preserve viral genome integrity
Article Snippet: .. Genomic DNA was harvested from cells 3 days after induction with PMA/NaB and portions of the EBV genome were PCR amplified using PfuTurbo Cx Hotstart DNA Polymerase (Agilent 600410). .. PCR products correspond to regions near the genes BRRF2 (95085-95584), LMP1 (167979-168514), BHRF1 (42090-42595), and BcLF1 (124082-124598).

Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
Article Snippet: .. In order to determine the minimum number of PCR cycles required for final library amplification, 50 μl PCR reactions containing 3 μl bisulfite-converted DNA, 5 μl 10× PfuTurbo Cx hotstart DNA polymerase buffer, 0.5 μl 100 mM dNTP (25 mM each dNTP) (Agilent, 200415), 0.5 μl Illumina TruSeq PCR primers (25 μM each primer) and 1 μl PfuTurbo Cx hotstart DNA polymerase (Agilent, 600412) were prepared. .. Reactions were then split equally into four separate tubes and thermocycled using the following conditions: denature at 95 °C for 2 min followed by X cycles of 95 °C for 30 s, 65 °C for 30 s, 72 °C for 45 s (where X number of cycles = 11, 13, 15 and 17), followed by a final extension at 72 °C for 7 min. PCR products were purified using 1.2× volumes of Ampure XP and analysed on an Agilent Bioanalyzer using a High Sensitivity DNA kit (Agilent, 5067-4626).

Purification:

Article Title: Candidate genes responsible for early key events of phenobarbital-promoted mouse hepatocellular tumorigenesis based on differentiation of regulating genes between wild type mice and humanized chimeric mice genes responsible for early key events of phenobarbital-promoted mouse hepatocellular tumorigenesis based on differentiation of regulating genes
Article Snippet: .. Immunoprecipitated DNA and input DNA were purified as described above, and amplified using Primer-3 (see ESI Table S1 ) for 17 cycles in a reaction containing 400 μM dATP, 400 μM dCTP, 400 μM dGTP, 320 μM dTTP, 80 μM dUTP, PfuTurbo Cx Hotstart DNA Polymerase (Agilent Technologies) and 10× reaction buffer. .. Amplified DNA was purified as described above.

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  • 90
    Agilent technologies pfuturbo cx hotstart dna polymerase
    Pfuturbo Cx Hotstart Dna Polymerase, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfuturbo cx hotstart dna polymerase/product/Agilent technologies
    Average 90 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    pfuturbo cx hotstart dna polymerase - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    86
    Agilent technologies pfuturbo cx hotstart dna polymeras
    Pfuturbo Cx Hotstart Dna Polymeras, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfuturbo cx hotstart dna polymeras/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pfuturbo cx hotstart dna polymeras - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

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