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Agilent technologies pfuturbo cx hotstart dna polymerase buffer
Pfuturbo Cx Hotstart Dna Polymerase Buffer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pfuturbo cx hotstart dna polymerase buffer/product/Agilent technologies
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
pfuturbo cx hotstart dna polymerase buffer - by Bioz Stars, 2020-07
93/100 stars

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Polymerase Chain Reaction:

Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
Article Snippet: .. In order to determine the minimum number of PCR cycles required for final library amplification, 50 μl PCR reactions containing 3 μl bisulfite-converted DNA, 5 μl 10× PfuTurbo Cx hotstart DNA polymerase buffer, 0.5 μl 100 mM dNTP (25 mM each dNTP) (Agilent, 200415), 0.5 μl Illumina TruSeq PCR primers (25 μM each primer) and 1 μl PfuTurbo Cx hotstart DNA polymerase (Agilent, 600412) were prepared. .. Reactions were then split equally into four separate tubes and thermocycled using the following conditions: denature at 95 °C for 2 min followed by X cycles of 95 °C for 30 s, 65 °C for 30 s, 72 °C for 45 s (where X number of cycles = 11, 13, 15 and 17), followed by a final extension at 72 °C for 7 min. PCR products were purified using 1.2× volumes of Ampure XP and analysed on an Agilent Bioanalyzer using a High Sensitivity DNA kit (Agilent, 5067-4626).

Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
Article Snippet: .. In order to determine the minimum number of PCR cycles required for final library amplification, 50 μl PCR reactions containing 3 μl bisulfite-converted DNA, 5 μl 10× PfuTurbo Cx hotstart DNA polymerase buffer, 0.5 μl 100 mM dNTP (25 mM each dNTP) (Agilent, 200415), 0.5 μl Illumina TruSeq PCR primers (25 μM each primer) and 1 μl PfuTurbo Cx hotstart DNA polymerase (Agilent, 600412) were prepared. .. Reactions were then split equally into four separate tubes and thermocycled using the following conditions: denature at 95 °C for 2 min followed by X cycles of 95 °C for 30 s, 65 °C for 30 s, 72 °C for 45 s (where X number of cycles = 11, 13, 15 and 17), followed by a final extension at 72 °C for 7 min. PCR products were purified using 1.2× volumes of Ampure XP and analysed on an Agilent Bioanalyzer using a High Sensitivity DNA kit (Agilent, 5067-4626).

Amplification:

Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
Article Snippet: .. In order to determine the minimum number of PCR cycles required for final library amplification, 50 μl PCR reactions containing 3 μl bisulfite-converted DNA, 5 μl 10× PfuTurbo Cx hotstart DNA polymerase buffer, 0.5 μl 100 mM dNTP (25 mM each dNTP) (Agilent, 200415), 0.5 μl Illumina TruSeq PCR primers (25 μM each primer) and 1 μl PfuTurbo Cx hotstart DNA polymerase (Agilent, 600412) were prepared. .. Reactions were then split equally into four separate tubes and thermocycled using the following conditions: denature at 95 °C for 2 min followed by X cycles of 95 °C for 30 s, 65 °C for 30 s, 72 °C for 45 s (where X number of cycles = 11, 13, 15 and 17), followed by a final extension at 72 °C for 7 min. PCR products were purified using 1.2× volumes of Ampure XP and analysed on an Agilent Bioanalyzer using a High Sensitivity DNA kit (Agilent, 5067-4626).

Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
Article Snippet: .. In order to determine the minimum number of PCR cycles required for final library amplification, 50 μl PCR reactions containing 3 μl bisulfite-converted DNA, 5 μl 10× PfuTurbo Cx hotstart DNA polymerase buffer, 0.5 μl 100 mM dNTP (25 mM each dNTP) (Agilent, 200415), 0.5 μl Illumina TruSeq PCR primers (25 μM each primer) and 1 μl PfuTurbo Cx hotstart DNA polymerase (Agilent, 600412) were prepared. .. Reactions were then split equally into four separate tubes and thermocycled using the following conditions: denature at 95 °C for 2 min followed by X cycles of 95 °C for 30 s, 65 °C for 30 s, 72 °C for 45 s (where X number of cycles = 11, 13, 15 and 17), followed by a final extension at 72 °C for 7 min. PCR products were purified using 1.2× volumes of Ampure XP and analysed on an Agilent Bioanalyzer using a High Sensitivity DNA kit (Agilent, 5067-4626).

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  • 93
    Agilent technologies pfuturbo cx hotstart dna polymerase buffer
    Pfuturbo Cx Hotstart Dna Polymerase Buffer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfuturbo cx hotstart dna polymerase buffer/product/Agilent technologies
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pfuturbo cx hotstart dna polymerase buffer - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    90
    Agilent technologies pfuturbo cx hotstart dna polymerase
    Pfuturbo Cx Hotstart Dna Polymerase, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfuturbo cx hotstart dna polymerase/product/Agilent technologies
    Average 90 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    pfuturbo cx hotstart dna polymerase - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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