pfuse2 clig rk1 vectors  (InvivoGen)

 
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    Name:
    pFUSE2 CLIg rK1
    Description:
    Rabbit kappa 1
    Catalog Number:
    pfuse2-rclk1
    Price:
    None
    Size:
    20 µg
    Category:
    pFUSE2 CLIg rK1 rabbit pFUSE CLIg pFUSE Collection Antibody Generation Antibodies ELISAs
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    Structured Review

    InvivoGen pfuse2 clig rk1 vectors
    Rabbit kappa 1
    https://www.bioz.com/result/pfuse2 clig rk1 vectors/product/InvivoGen
    Average 83 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pfuse2 clig rk1 vectors - by Bioz Stars, 2020-01
    83/100 stars

    Related Products / Commonly Used Together

    pcr
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    Related Articles

    Clone Assay:

    Article Title: A structural basis for antibody-mediated neutralization of Nipah virus reveals a site of vulnerability at the fusion glycoprotein apex
    Article Snippet: .. PCR products were purified and cloned into an expression plasmid ( , ) adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs) under ampicillin selection following the manufacturer’s protocol. .. Antibody heavy and light plasmids were cotransfected at a 1:1 ratio into HEK293F cells (ThermoFisher) using PEI Max 40K (linear polyethylenimine hydrochloride, Polysciences).

    Transfection:

    Article Title: A structural basis for antibody-mediated neutralization of Nipah virus reveals a site of vulnerability at the fusion glycoprotein apex
    Article Snippet: PCR products were purified and cloned into an expression plasmid ( , ) adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs) under ampicillin selection following the manufacturer’s protocol. .. Antibody supernatant was harvested 4 d following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare).

    Selection:

    Article Title: A structural basis for antibody-mediated neutralization of Nipah virus reveals a site of vulnerability at the fusion glycoprotein apex
    Article Snippet: .. PCR products were purified and cloned into an expression plasmid ( , ) adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs) under ampicillin selection following the manufacturer’s protocol. .. Antibody heavy and light plasmids were cotransfected at a 1:1 ratio into HEK293F cells (ThermoFisher) using PEI Max 40K (linear polyethylenimine hydrochloride, Polysciences).

    Cell Culture:

    Article Title: A structural basis for antibody-mediated neutralization of Nipah virus reveals a site of vulnerability at the fusion glycoprotein apex
    Article Snippet: Hybridomas were cultured in medium E (Clonacell) at 37 °C with 5% humidity. .. PCR products were purified and cloned into an expression plasmid ( , ) adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs) under ampicillin selection following the manufacturer’s protocol.

    Purification:

    Article Title: A structural basis for antibody-mediated neutralization of Nipah virus reveals a site of vulnerability at the fusion glycoprotein apex
    Article Snippet: .. PCR products were purified and cloned into an expression plasmid ( , ) adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs) under ampicillin selection following the manufacturer’s protocol. .. Antibody heavy and light plasmids were cotransfected at a 1:1 ratio into HEK293F cells (ThermoFisher) using PEI Max 40K (linear polyethylenimine hydrochloride, Polysciences).

    Polymerase Chain Reaction:

    Article Title: A structural basis for antibody-mediated neutralization of Nipah virus reveals a site of vulnerability at the fusion glycoprotein apex
    Article Snippet: .. PCR products were purified and cloned into an expression plasmid ( , ) adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs) under ampicillin selection following the manufacturer’s protocol. .. Antibody heavy and light plasmids were cotransfected at a 1:1 ratio into HEK293F cells (ThermoFisher) using PEI Max 40K (linear polyethylenimine hydrochloride, Polysciences).

    Generated:

    Article Title: A structural basis for antibody-mediated neutralization of Nipah virus reveals a site of vulnerability at the fusion glycoprotein apex
    Article Snippet: When cells achieved appropriate density, total RNA was extracted using the Qiagen RNeasy mini kit, per the manufacturer’s protocol. cDNA was generated using the Invitrogen SuperScript IV First Strand Synthesis System using random hexamers following the manufacturer’s protocol. .. PCR products were purified and cloned into an expression plasmid ( , ) adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs) under ampicillin selection following the manufacturer’s protocol.

    Affinity Chromatography:

    Article Title: A structural basis for antibody-mediated neutralization of Nipah virus reveals a site of vulnerability at the fusion glycoprotein apex
    Article Snippet: PCR products were purified and cloned into an expression plasmid ( , ) adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs) under ampicillin selection following the manufacturer’s protocol. .. Antibody supernatant was harvested 4 d following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare).

    Expressing:

    Article Title: A structural basis for antibody-mediated neutralization of Nipah virus reveals a site of vulnerability at the fusion glycoprotein apex
    Article Snippet: .. PCR products were purified and cloned into an expression plasmid ( , ) adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs) under ampicillin selection following the manufacturer’s protocol. .. Antibody heavy and light plasmids were cotransfected at a 1:1 ratio into HEK293F cells (ThermoFisher) using PEI Max 40K (linear polyethylenimine hydrochloride, Polysciences).

    Sequencing:

    Article Title: A structural basis for antibody-mediated neutralization of Nipah virus reveals a site of vulnerability at the fusion glycoprotein apex
    Article Snippet: Paragraph title: Sequencing and Cloning of mAb66 Variable Regions from Hybridoma Cell Line. ... PCR products were purified and cloned into an expression plasmid ( , ) adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs) under ampicillin selection following the manufacturer’s protocol.

    Plasmid Preparation:

    Article Title: A structural basis for antibody-mediated neutralization of Nipah virus reveals a site of vulnerability at the fusion glycoprotein apex
    Article Snippet: .. PCR products were purified and cloned into an expression plasmid ( , ) adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs) under ampicillin selection following the manufacturer’s protocol. .. Antibody heavy and light plasmids were cotransfected at a 1:1 ratio into HEK293F cells (ThermoFisher) using PEI Max 40K (linear polyethylenimine hydrochloride, Polysciences).

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  • 83
    InvivoGen pfuse2 clig rk1 vectors
    Pfuse2 Clig Rk1 Vectors, supplied by InvivoGen, used in various techniques. Bioz Stars score: 83/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfuse2 clig rk1 vectors/product/InvivoGen
    Average 83 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pfuse2 clig rk1 vectors - by Bioz Stars, 2020-01
    83/100 stars
      Buy from Supplier

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