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InvivoGen pfuse rigg fc
Pfuse Rigg Fc, supplied by InvivoGen, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pfuse rigg fc/product/InvivoGen
Average 91 stars, based on 5 article reviews
Price from $9.99 to $1999.99
pfuse rigg fc - by Bioz Stars, 2020-01
91/100 stars

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Amplification:

Article Title: A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus
Article Snippet: Full-length Ab cloning and expression The rabbit Ab variable regions of heavy and kappa chains were PCR amplified using previously described primers and PCR conditions ( ) ( ). .. PCR products were purified and cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly® Master Mix (NEB) under ampicillin selection following the manufacturer’s protocol.

Clone Assay:

Article Title: A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus
Article Snippet: .. PCR products were purified and cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly® Master Mix (NEB) under ampicillin selection following the manufacturer’s protocol. .. Ab variable regions were sequenced by Sanger sequencing.

Transfection:

Article Title: A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus
Article Snippet: PCR products were purified and cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly® Master Mix (NEB) under ampicillin selection following the manufacturer’s protocol. .. Ab supernatants were harvested four days following transfection and purified using protein G affinity chromatography following the maunfacturers protocol (GE Healthcare).

Selection:

Article Title: A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus
Article Snippet: .. PCR products were purified and cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly® Master Mix (NEB) under ampicillin selection following the manufacturer’s protocol. .. Ab variable regions were sequenced by Sanger sequencing.

Purification:

Article Title: A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus
Article Snippet: .. PCR products were purified and cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly® Master Mix (NEB) under ampicillin selection following the manufacturer’s protocol. .. Ab variable regions were sequenced by Sanger sequencing.

Polymerase Chain Reaction:

Article Title: A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus
Article Snippet: .. PCR products were purified and cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly® Master Mix (NEB) under ampicillin selection following the manufacturer’s protocol. .. Ab variable regions were sequenced by Sanger sequencing.

Generated:

Article Title: A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus
Article Snippet: PCR products were purified and cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly® Master Mix (NEB) under ampicillin selection following the manufacturer’s protocol. .. Ab heavy and light plasmids generated through B cell sorting were co-transfected at a 1:1 ratio into HEK293F cells (Thermofisher) using PEI Max 40K (linear polyethylenimine hydrochloride, Polysciences, Inc.).

Affinity Chromatography:

Article Title: A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus
Article Snippet: PCR products were purified and cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly® Master Mix (NEB) under ampicillin selection following the manufacturer’s protocol. .. Ab supernatants were harvested four days following transfection and purified using protein G affinity chromatography following the maunfacturers protocol (GE Healthcare).

Expressing:

Article Title: A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus
Article Snippet: .. PCR products were purified and cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly® Master Mix (NEB) under ampicillin selection following the manufacturer’s protocol. .. Ab variable regions were sequenced by Sanger sequencing.

Sequencing:

Article Title: A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus
Article Snippet: PCR products were purified and cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly® Master Mix (NEB) under ampicillin selection following the manufacturer’s protocol. .. Ab variable regions were sequenced by Sanger sequencing.

FACS:

Article Title: A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus
Article Snippet: PCR products were purified and cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly® Master Mix (NEB) under ampicillin selection following the manufacturer’s protocol. .. Ab heavy and light plasmids generated through B cell sorting were co-transfected at a 1:1 ratio into HEK293F cells (Thermofisher) using PEI Max 40K (linear polyethylenimine hydrochloride, Polysciences, Inc.).

Plasmid Preparation:

Article Title: A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus
Article Snippet: .. PCR products were purified and cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 vectors (InvivoGen) using the Gibson Assembly® Master Mix (NEB) under ampicillin selection following the manufacturer’s protocol. .. Ab variable regions were sequenced by Sanger sequencing.

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    InvivoGen pfuse rfc2
    Pfuse Rfc2, supplied by InvivoGen, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfuse rfc2/product/InvivoGen
    Average 86 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pfuse rfc2 - by Bioz Stars, 2020-01
    86/100 stars
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