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Thermo Fisher pfoa
Spike raster plots illustrating the pattern of activity of a representative well of the iCell Glutaneuron – iCell Astrocytes co-culture before exposure (left) and the same well following exposure (right) to <t>PFOS</t> 10 µM ( A ) or <t>PFOA</t> 100 µM ( B ). Each row depicts one electrode in a well and each tick mark represents one spike in a 50 s interval. Spikes are depicted in black and bursts are depicted in blue.
Pfoa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pfoa/product/Thermo Fisher
Average 99 stars, based on 2 article reviews
Price from $9.99 to $1999.99
pfoa - by Bioz Stars, 2020-09
99/100 stars

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1) Product Images from "Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro"

Article Title: Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro

Journal: Scientific Reports

doi: 10.1038/s41598-020-62152-2

Spike raster plots illustrating the pattern of activity of a representative well of the iCell Glutaneuron – iCell Astrocytes co-culture before exposure (left) and the same well following exposure (right) to PFOS 10 µM ( A ) or PFOA 100 µM ( B ). Each row depicts one electrode in a well and each tick mark represents one spike in a 50 s interval. Spikes are depicted in black and bursts are depicted in blue.
Figure Legend Snippet: Spike raster plots illustrating the pattern of activity of a representative well of the iCell Glutaneuron – iCell Astrocytes co-culture before exposure (left) and the same well following exposure (right) to PFOS 10 µM ( A ) or PFOA 100 µM ( B ). Each row depicts one electrode in a well and each tick mark represents one spike in a 50 s interval. Spikes are depicted in black and bursts are depicted in blue.

Techniques Used: Activity Assay, Co-Culture Assay

Modulation of spontaneous neuronal activity of rat primary cortical neurons exposed to PFOS (black) or PFOA (grey) following acute exposure. Effects on mean spike rate (MSR; A ), mean burst rate (MBR; B ), mean network burst rate (MNBR; C ), burst duration ( D ) and network burst duration ( E ) are depicted as average in % change of control (solvent control set to 100%; dashed line) ± SEM from n = 18–20 wells and N = 4 plates. *Indicates p
Figure Legend Snippet: Modulation of spontaneous neuronal activity of rat primary cortical neurons exposed to PFOS (black) or PFOA (grey) following acute exposure. Effects on mean spike rate (MSR; A ), mean burst rate (MBR; B ), mean network burst rate (MNBR; C ), burst duration ( D ) and network burst duration ( E ) are depicted as average in % change of control (solvent control set to 100%; dashed line) ± SEM from n = 18–20 wells and N = 4 plates. *Indicates p

Techniques Used: Activity Assay

Modulation of spontaneous neuronal activity of iCell Glutaneuron – iCell Astrocytes co-culture exposed to PFOS (black) or PFOA (grey) following acute exposure. Effects on mean spike rate (MSR; A ), mean burst rate (MBR; B ), mean network burst rate (MNBR; C ), burst duration ( D ) and network burst duration ( E ) are depicted as average in % change of control (solvent control set to 100%; dashed line) ± SEM from n = 3–17 wells and N = 3–5 plates. *Indicates p
Figure Legend Snippet: Modulation of spontaneous neuronal activity of iCell Glutaneuron – iCell Astrocytes co-culture exposed to PFOS (black) or PFOA (grey) following acute exposure. Effects on mean spike rate (MSR; A ), mean burst rate (MBR; B ), mean network burst rate (MNBR; C ), burst duration ( D ) and network burst duration ( E ) are depicted as average in % change of control (solvent control set to 100%; dashed line) ± SEM from n = 3–17 wells and N = 3–5 plates. *Indicates p

Techniques Used: Activity Assay, Co-Culture Assay

Modulation of spontaneous neuronal activity of SynFire iNS co-culture with PFOS (black) or PFOA (grey). Effects on mean spike rate (MSR; A ), mean burst rate (MBR; B ), mean network burst rate (MNBR; C ), burst duration ( D ) and network burst duration ( E ) are depicted as average in % change of control (solvent control set to 100%; dashed line) ± SEM from n = 7–9 wells and N = 2 plates. *Indicates p
Figure Legend Snippet: Modulation of spontaneous neuronal activity of SynFire iNS co-culture with PFOS (black) or PFOA (grey). Effects on mean spike rate (MSR; A ), mean burst rate (MBR; B ), mean network burst rate (MNBR; C ), burst duration ( D ) and network burst duration ( E ) are depicted as average in % change of control (solvent control set to 100%; dashed line) ± SEM from n = 7–9 wells and N = 2 plates. *Indicates p

Techniques Used: Activity Assay, Co-Culture Assay

Antagonistic effects of PFOS and PFOA on the human GABA A receptor. Example recordings of inhibition of GABA-evoked ion currents by co-application of PFOS (1 µM, a , c ) or PFOA (10 µM, b , d ) with GABA (at low and high effective GABA concentration, respectively). PFOS-induced inhibition is poorly reversible ( a , c ), whereas PFOA-induced inhibition is reversed within seconds ( b , d ). Scale bar applies to all traces. Concentration-response curves show the concentration-dependent inhibition of GABA-evoked responses by PFOS ( e ) and PFOA ( f ) on the human GAB A receptor with at low ( e , f ; solid line) and high ( e,f ; dashed line) effective GABA concentrations. Inhibition is presented as percentage of the GABA-evoked response (mean ± SEM, n = 3–4 oocytes per concentration from N = 1–2 batches).
Figure Legend Snippet: Antagonistic effects of PFOS and PFOA on the human GABA A receptor. Example recordings of inhibition of GABA-evoked ion currents by co-application of PFOS (1 µM, a , c ) or PFOA (10 µM, b , d ) with GABA (at low and high effective GABA concentration, respectively). PFOS-induced inhibition is poorly reversible ( a , c ), whereas PFOA-induced inhibition is reversed within seconds ( b , d ). Scale bar applies to all traces. Concentration-response curves show the concentration-dependent inhibition of GABA-evoked responses by PFOS ( e ) and PFOA ( f ) on the human GAB A receptor with at low ( e , f ; solid line) and high ( e,f ; dashed line) effective GABA concentrations. Inhibition is presented as percentage of the GABA-evoked response (mean ± SEM, n = 3–4 oocytes per concentration from N = 1–2 batches).

Techniques Used: Inhibition, Concentration Assay

Heatmap of the effects of PFOS (light green) and PFOA (darker green) on selected metric parameters on rat primary cortical neurons (top; grey), iCell Glutaneuron – iCell Astrocytes (middle; black) and SynFire iNS co-culture (bottom; green). Colour scaling is based on the magnitude change in percentages relative to vehicle control based on n = 18–20 wells and N = 4 plates for rat primary cortical neurons; n = 3–17 wells and N = 3–5 plates for iCell Glutaneuron – iCell Astrocytes; n = 6–9 wells N = 2 plates for SynFire iNS co-culture.
Figure Legend Snippet: Heatmap of the effects of PFOS (light green) and PFOA (darker green) on selected metric parameters on rat primary cortical neurons (top; grey), iCell Glutaneuron – iCell Astrocytes (middle; black) and SynFire iNS co-culture (bottom; green). Colour scaling is based on the magnitude change in percentages relative to vehicle control based on n = 18–20 wells and N = 4 plates for rat primary cortical neurons; n = 3–17 wells and N = 3–5 plates for iCell Glutaneuron – iCell Astrocytes; n = 6–9 wells N = 2 plates for SynFire iNS co-culture.

Techniques Used: Co-Culture Assay

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Concentration Assay:

Article Title: Critical Synergistic Concentration of Lecithin Phospholipids Improves the Antimicrobial Activity of Eugenol against Escherichia coli
Article Snippet: .. A 0.043% (vol/vol) eugenol concentration (previously shown to reduce the population of E. coli C600 at least 0.2 log CFU/ml after a 30-min exposure [ ]) dispersed in PBS-phospholipid was prepared by suspending 43 μl eugenol (Acros Oganics, Fairlawn, NJ) in 99 ml dilute phospholipid solution, followed by rotary homogenization (Ultra-Turrax IKA T-18 basic; Staufen, Germany) at 11,000 rpm for 3 min. .. After mixing a 1-ml working culture (without washing) with 99 ml eugenol-phospholipid mixtures (initial inoculum concentration ca.

Homogenization:

Article Title: Critical Synergistic Concentration of Lecithin Phospholipids Improves the Antimicrobial Activity of Eugenol against Escherichia coli
Article Snippet: .. A 0.043% (vol/vol) eugenol concentration (previously shown to reduce the population of E. coli C600 at least 0.2 log CFU/ml after a 30-min exposure [ ]) dispersed in PBS-phospholipid was prepared by suspending 43 μl eugenol (Acros Oganics, Fairlawn, NJ) in 99 ml dilute phospholipid solution, followed by rotary homogenization (Ultra-Turrax IKA T-18 basic; Staufen, Germany) at 11,000 rpm for 3 min. .. After mixing a 1-ml working culture (without washing) with 99 ml eugenol-phospholipid mixtures (initial inoculum concentration ca.

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    Thermo Fisher pfoa
    Pfoa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfoa/product/Thermo Fisher
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pfoa - by Bioz Stars, 2020-09
    91/100 stars
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