peu8 2 vh vector  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91

    Structured Review

    New England Biolabs peu8 2 vh vector
    Peu8 2 Vh Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peu8 2 vh vector/product/New England Biolabs
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    peu8 2 vh vector - by Bioz Stars, 2020-01
    91/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: .. In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector. .. Stellar Competent Cells (Clontech Laboratories, 636,763) were transformed with the obtained vectors, and the colonies were screened by digestion and sequence analysis.

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: .. In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector. .. Stellar Competent Cells (Clontech Laboratories, 636,763) were transformed with the obtained vectors, and the colonies were screened by digestion and sequence analysis.

    Transfection:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector. .. The vectors containing the correct DNA, prepared with an endotoxin-free system (EndoFree Plasmid Maxi Kit, Qiagen, 12,362), were co-transfected in HEK293-EBNA by using Lipofectamine Transfection Reagent (Life Technologies, Inc. 11,668,019) and grown up for about 10 days at 37°C in chemical defined CD CHO medium (Gibco, Life Technologies, Inc. 10,743,029) supplemented with 5 mL of L-glutamine 200 mM (Gibco, Life Technologies, A2916801), 5 mL of Penicillin-Streptomicyn 10,000 U/mL-10 mg/mL (Sigma-Aldirch, P0781) in 6-well plates or in 150 mm Corning® tissue-culture treated culture dishes.

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector. .. The vectors containing the correct DNA, prepared with an endotoxin-free system (EndoFree Plasmid Maxi Kit, Qiagen, 12,362), were co-transfected in HEK293-EBNA by using Lipofectamine Transfection Reagent (Life Technologies, Inc. 11,668,019) and grown up for about 10 days at 37°C in chemical defined CD CHO medium (Gibco, Life Technologies, Inc. 10,743,029) supplemented with 5 mL of L-glutamine 200 mM (Gibco, Life Technologies, A2916801), 5 mL of Penicillin-Streptomicyn 10,000 U/mL-10 mg/mL (Sigma-Aldirch, P0781) in 6-well plates or in 150 mm Corning® tissue-culture treated culture dishes.

    Amplification:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: Briefly, the VHs and VLs were amplified by CloneAmp HiFi PCR Premix in standard conditions with specific primers and purified with Wizard® SV Gel and PCR Clean-Up System (Promega, A9281) from 1,3% agarose gel. .. In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector.

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: Briefly, the VHs and VLs were amplified by CloneAmp HiFi PCR Premix in standard conditions with specific primers and purified with Wizard® SV Gel and PCR Clean-Up System (Promega, A9281) from 1,3% agarose gel. .. In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector.

    Agarose Gel Electrophoresis:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: Briefly, the VHs and VLs were amplified by CloneAmp HiFi PCR Premix in standard conditions with specific primers and purified with Wizard® SV Gel and PCR Clean-Up System (Promega, A9281) from 1,3% agarose gel. .. In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector.

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: Briefly, the VHs and VLs were amplified by CloneAmp HiFi PCR Premix in standard conditions with specific primers and purified with Wizard® SV Gel and PCR Clean-Up System (Promega, A9281) from 1,3% agarose gel. .. In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector.

    Purification:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: Paragraph title: Antibody production and purification ... In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector.

    Polymerase Chain Reaction:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: Briefly, the VHs and VLs were amplified by CloneAmp HiFi PCR Premix in standard conditions with specific primers and purified with Wizard® SV Gel and PCR Clean-Up System (Promega, A9281) from 1,3% agarose gel. .. In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector.

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: Briefly, the VHs and VLs were amplified by CloneAmp HiFi PCR Premix in standard conditions with specific primers and purified with Wizard® SV Gel and PCR Clean-Up System (Promega, A9281) from 1,3% agarose gel. .. In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector.

    Expressing:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: Antibody production and purification The scFvs of interest were converted into whole IgG4 antibodies by cloning the corresponding VH and VL cDNAs in the pEU vectors 8.2VH and 4.2VL, expressing respectively, the constant antibody heavy and light chains . .. In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector.

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: The scFvs of interest were converted into whole IgG4 antibodies by cloning the corresponding VH and VL cDNAs in the pEU vectors 8.2VH and 4.2VL, expressing respectively, the constant antibody heavy and light chains . .. In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector.

    Sequencing:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector. .. Stellar Competent Cells (Clontech Laboratories, 636,763) were transformed with the obtained vectors, and the colonies were screened by digestion and sequence analysis.

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector. .. Stellar Competent Cells (Clontech Laboratories, 636,763) were transformed with the obtained vectors, and the colonies were screened by digestion and sequence analysis.

    Transformation Assay:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector. .. Stellar Competent Cells (Clontech Laboratories, 636,763) were transformed with the obtained vectors, and the colonies were screened by digestion and sequence analysis.

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector. .. Stellar Competent Cells (Clontech Laboratories, 636,763) were transformed with the obtained vectors, and the colonies were screened by digestion and sequence analysis.

    Plasmid Preparation:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: .. In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector. .. Stellar Competent Cells (Clontech Laboratories, 636,763) were transformed with the obtained vectors, and the colonies were screened by digestion and sequence analysis.

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: .. In-Fusion HD cloning kit (Clontech Laboratories, 639,692) was used to clone the VHs in Bam HI (R3136) and Bss HII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in Apa LI (R0507) and Avr II (R0174) New England Biolabs, linearized pEU4.2VL vector. .. Stellar Competent Cells (Clontech Laboratories, 636,763) were transformed with the obtained vectors, and the colonies were screened by digestion and sequence analysis.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    New England Biolabs peu8 2 vh vector
    Peu8 2 Vh Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peu8 2 vh vector/product/New England Biolabs
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    peu8 2 vh vector - by Bioz Stars, 2020-01
    91/100 stars
      Buy from Supplier

    Image Search Results