pet21  (New England Biolabs)


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    New England Biolabs pet21
    Functional analysis of mutated MukB neck variants. in vivo complementation in cells lacking chromosomal mukB gene by variants expressed from <t>pET21</t> plasmid. Growth of material streaked from 6 colonies of each variant was compared to growth of cells carrying WT MukB construct at permissive, 22°C, and non-permissive, 37°C; ϕ - a negative control, empty vector.
    Pet21, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet21/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pet21 - by Bioz Stars, 2022-07
    95/100 stars

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    1) Product Images from "MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin"

    Article Title: MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin

    Journal: eLife

    doi: 10.7554/eLife.31522

    Functional analysis of mutated MukB neck variants. in vivo complementation in cells lacking chromosomal mukB gene by variants expressed from pET21 plasmid. Growth of material streaked from 6 colonies of each variant was compared to growth of cells carrying WT MukB construct at permissive, 22°C, and non-permissive, 37°C; ϕ - a negative control, empty vector.
    Figure Legend Snippet: Functional analysis of mutated MukB neck variants. in vivo complementation in cells lacking chromosomal mukB gene by variants expressed from pET21 plasmid. Growth of material streaked from 6 colonies of each variant was compared to growth of cells carrying WT MukB construct at permissive, 22°C, and non-permissive, 37°C; ϕ - a negative control, empty vector.

    Techniques Used: Functional Assay, In Vivo, Plasmid Preparation, Variant Assay, Construct, Negative Control

    Functional analysis of mutated MukF helix9 variants. in vivo complementation in strain lacking chromosomal mukF gene by variants expressed from pET21 was assessed in the absence of IPTG (constitutive leaky expression). Growth material from colonies of each variant and MukF was streaked on LB and incubated at permissive, 22°C, and non-permissive, 37°C temperature and compared to negative control, empty vector.
    Figure Legend Snippet: Functional analysis of mutated MukF helix9 variants. in vivo complementation in strain lacking chromosomal mukF gene by variants expressed from pET21 was assessed in the absence of IPTG (constitutive leaky expression). Growth material from colonies of each variant and MukF was streaked on LB and incubated at permissive, 22°C, and non-permissive, 37°C temperature and compared to negative control, empty vector.

    Techniques Used: Functional Assay, In Vivo, Expressing, Variant Assay, Incubation, Negative Control, Plasmid Preparation

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    New England Biolabs pet21
    Functional analysis of mutated MukB neck variants. in vivo complementation in cells lacking chromosomal mukB gene by variants expressed from <t>pET21</t> plasmid. Growth of material streaked from 6 colonies of each variant was compared to growth of cells carrying WT MukB construct at permissive, 22°C, and non-permissive, 37°C; ϕ - a negative control, empty vector.
    Pet21, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet21/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pet21 - by Bioz Stars, 2022-07
    95/100 stars
      Buy from Supplier

    86
    New England Biolabs pet21 mruby2 vector
    Functional analysis of mutated MukB neck variants. in vivo complementation in cells lacking chromosomal mukB gene by variants expressed from <t>pET21</t> plasmid. Growth of material streaked from 6 colonies of each variant was compared to growth of cells carrying WT MukB construct at permissive, 22°C, and non-permissive, 37°C; ϕ - a negative control, empty vector.
    Pet21 Mruby2 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet21 mruby2 vector/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pet21 mruby2 vector - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

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    Functional analysis of mutated MukB neck variants. in vivo complementation in cells lacking chromosomal mukB gene by variants expressed from pET21 plasmid. Growth of material streaked from 6 colonies of each variant was compared to growth of cells carrying WT MukB construct at permissive, 22°C, and non-permissive, 37°C; ϕ - a negative control, empty vector.

    Journal: eLife

    Article Title: MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin

    doi: 10.7554/eLife.31522

    Figure Lengend Snippet: Functional analysis of mutated MukB neck variants. in vivo complementation in cells lacking chromosomal mukB gene by variants expressed from pET21 plasmid. Growth of material streaked from 6 colonies of each variant was compared to growth of cells carrying WT MukB construct at permissive, 22°C, and non-permissive, 37°C; ϕ - a negative control, empty vector.

    Article Snippet: MukF variants and MukE 6xHis-tagged at the C-terminus were expressed from pET21 in C3013I cells (NEB).

    Techniques: Functional Assay, In Vivo, Plasmid Preparation, Variant Assay, Construct, Negative Control

    Functional analysis of mutated MukF helix9 variants. in vivo complementation in strain lacking chromosomal mukF gene by variants expressed from pET21 was assessed in the absence of IPTG (constitutive leaky expression). Growth material from colonies of each variant and MukF was streaked on LB and incubated at permissive, 22°C, and non-permissive, 37°C temperature and compared to negative control, empty vector.

    Journal: eLife

    Article Title: MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin

    doi: 10.7554/eLife.31522

    Figure Lengend Snippet: Functional analysis of mutated MukF helix9 variants. in vivo complementation in strain lacking chromosomal mukF gene by variants expressed from pET21 was assessed in the absence of IPTG (constitutive leaky expression). Growth material from colonies of each variant and MukF was streaked on LB and incubated at permissive, 22°C, and non-permissive, 37°C temperature and compared to negative control, empty vector.

    Article Snippet: MukF variants and MukE 6xHis-tagged at the C-terminus were expressed from pET21 in C3013I cells (NEB).

    Techniques: Functional Assay, In Vivo, Expressing, Variant Assay, Incubation, Negative Control, Plasmid Preparation

    (A)The ATPase activities (Mean ± SEM) of MukB, S718TAG MukB, and Cy5(Cy3) labelled MukB, in the presence of the indicated components. (B) Functional analysis by complementation assay of mutated MukB_S718S. In vivo complementation in cells lacking chromosomal MukB gene ΔmukB (RRL149) by variants expressed from pET21 plasmid. Growth of material streaked of MukB_S718F was compared to growth of cells carrying WT-MukB construct at permissive, 22°C, and non-permissive, 37°C; Control: empty vector, a negative control. The MukB_S718F fully complements the temperature-sensitive growth defect of ΔmukB cells in vivo .

    Journal: bioRxiv

    Article Title: DNA sliding and loop formation by E. coli SMC complex: MukBEF

    doi: 10.1101/2021.07.17.452765

    Figure Lengend Snippet: (A)The ATPase activities (Mean ± SEM) of MukB, S718TAG MukB, and Cy5(Cy3) labelled MukB, in the presence of the indicated components. (B) Functional analysis by complementation assay of mutated MukB_S718S. In vivo complementation in cells lacking chromosomal MukB gene ΔmukB (RRL149) by variants expressed from pET21 plasmid. Growth of material streaked of MukB_S718F was compared to growth of cells carrying WT-MukB construct at permissive, 22°C, and non-permissive, 37°C; Control: empty vector, a negative control. The MukB_S718F fully complements the temperature-sensitive growth defect of ΔmukB cells in vivo .

    Article Snippet: Expression and purification of the MukBEF His6 -tagged proteins Wild-type MukB was 6×His-tagged at the C-terminus and was expressed from plasmid Pet21 in C3013I cells (NEB).

    Techniques: Functional Assay, In Vivo, Plasmid Preparation, Construct, Negative Control