pet15b bacterial expression vector  (Millipore)


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    Structured Review

    Millipore pet15b bacterial expression vector
    Pet15b Bacterial Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet15b bacterial expression vector/product/Millipore
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    pet15b bacterial expression vector - by Bioz Stars, 2020-07
    88/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Human amnion‐derived mesenchymal stem cells are a potential source for uterine stem cell therapy
    Article Snippet: .. Full‐length enhanced green fluorescence protein (EGFP) was amplified using pEGFP‐N1 vector (Invitrogen) as a template, and cloned into Xho I and Bam H1 sites of the pET15b bacterial expression vector (Novagen, Darmstadt, Germany). .. MPG peptide (GALFLGWLGAAGSTMGAPKKKRKV) sequence ( ) was amplified by Klenow reaction and was cloned into Nde I and Xho I sites in pET15b‐EGFP.

    Article Title: Nuclear localization of human spermine oxidase isoforms - possible implications in drug response and disease etiology
    Article Snippet: .. PAOh4 cDNA was cloned into the pET15b bacterial expression vector (Novagen, Madison, WI, USA) using the method previously described for SMO/PAOh1 [ ]. .. The resulting pET15b constructs, containing either SMO/PAOh1 or PAOh4, were then combined to produce SMO5/pET15b.

    Amplification:

    Article Title: Human amnion‐derived mesenchymal stem cells are a potential source for uterine stem cell therapy
    Article Snippet: .. Full‐length enhanced green fluorescence protein (EGFP) was amplified using pEGFP‐N1 vector (Invitrogen) as a template, and cloned into Xho I and Bam H1 sites of the pET15b bacterial expression vector (Novagen, Darmstadt, Germany). .. MPG peptide (GALFLGWLGAAGSTMGAPKKKRKV) sequence ( ) was amplified by Klenow reaction and was cloned into Nde I and Xho I sites in pET15b‐EGFP.

    In Vitro:

    Article Title: CSN5/JAB1 Interacts with the Centromeric Components CENP-T and CENP-W and Regulates Their Proteasome-mediated Degradation *
    Article Snippet: .. For the in vitro protein-binding assay, CSN5, CENP-W, and CENP-T were first subcloned into the pET15b bacterial expression vector (Novagen). .. Next, the His-tagged recombinant proteins were expressed using the Escherichia coli S30 T7 protein expression system (Promega), and co-immunoprecipitation was carried out using an anti-CSN5 antibody (Santa Cruz).

    Fluorescence:

    Article Title: Human amnion‐derived mesenchymal stem cells are a potential source for uterine stem cell therapy
    Article Snippet: .. Full‐length enhanced green fluorescence protein (EGFP) was amplified using pEGFP‐N1 vector (Invitrogen) as a template, and cloned into Xho I and Bam H1 sites of the pET15b bacterial expression vector (Novagen, Darmstadt, Germany). .. MPG peptide (GALFLGWLGAAGSTMGAPKKKRKV) sequence ( ) was amplified by Klenow reaction and was cloned into Nde I and Xho I sites in pET15b‐EGFP.

    Protein Binding:

    Article Title: CSN5/JAB1 Interacts with the Centromeric Components CENP-T and CENP-W and Regulates Their Proteasome-mediated Degradation *
    Article Snippet: .. For the in vitro protein-binding assay, CSN5, CENP-W, and CENP-T were first subcloned into the pET15b bacterial expression vector (Novagen). .. Next, the His-tagged recombinant proteins were expressed using the Escherichia coli S30 T7 protein expression system (Promega), and co-immunoprecipitation was carried out using an anti-CSN5 antibody (Santa Cruz).

    Polymerase Chain Reaction:

    Article Title: Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31
    Article Snippet: .. The DNA coding fragment for the Bap31 leucine-zipper region was obtained by standard PCR methods using a full-length human Bap31 clone as template (GenBank accession No. ) and subcloned into the Nde I/ Bam HI sites of a modified pET15b bacterial expression vector (Novagen), thus adding an N-­terminal 6×His tag to the protein (6×His-Met-Gly160). ..

    Expressing:

    Article Title: Low Molecular Weight Amidoximes that Act as Potent Inhibitors of Lysine-Specific Demethylase 1
    Article Snippet: .. Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. Following selection, expression and purification of recombinant LSD1 protein were performed as previously described.

    Article Title: (Bis)urea and (Bis)thiourea Inhibitors of Lysine-Specific Demethylase 1 as Epigenetic Modulators
    Article Snippet: .. Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. Following selection, expression and purification of recombinant LSD1 protein were performed as previously described.

    Article Title: A Novel Pyridoxal 5?-Phosphate-dependent Amino Acid Racemase in the Aplysia californica Central Nervous System *
    Article Snippet: .. The clone T90 plasmid and pET15b bacterial expression vector (EMD Chemicals) were double digested with NdeI and XhoI restriction enzymes (New England BioLabs, Inc.). .. The dar1 fragment and cleaved vector were gel-purified, ligated, and transformed into TOP10 cells.

    Article Title: Human amnion‐derived mesenchymal stem cells are a potential source for uterine stem cell therapy
    Article Snippet: .. Full‐length enhanced green fluorescence protein (EGFP) was amplified using pEGFP‐N1 vector (Invitrogen) as a template, and cloned into Xho I and Bam H1 sites of the pET15b bacterial expression vector (Novagen, Darmstadt, Germany). .. MPG peptide (GALFLGWLGAAGSTMGAPKKKRKV) sequence ( ) was amplified by Klenow reaction and was cloned into Nde I and Xho I sites in pET15b‐EGFP.

    Article Title: CSN5/JAB1 Interacts with the Centromeric Components CENP-T and CENP-W and Regulates Their Proteasome-mediated Degradation *
    Article Snippet: .. For the in vitro protein-binding assay, CSN5, CENP-W, and CENP-T were first subcloned into the pET15b bacterial expression vector (Novagen). .. Next, the His-tagged recombinant proteins were expressed using the Escherichia coli S30 T7 protein expression system (Promega), and co-immunoprecipitation was carried out using an anti-CSN5 antibody (Santa Cruz).

    Article Title: Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31
    Article Snippet: .. The DNA coding fragment for the Bap31 leucine-zipper region was obtained by standard PCR methods using a full-length human Bap31 clone as template (GenBank accession No. ) and subcloned into the Nde I/ Bam HI sites of a modified pET15b bacterial expression vector (Novagen), thus adding an N-­terminal 6×His tag to the protein (6×His-Met-Gly160). ..

    Article Title: Nuclear localization of human spermine oxidase isoforms - possible implications in drug response and disease etiology
    Article Snippet: .. PAOh4 cDNA was cloned into the pET15b bacterial expression vector (Novagen, Madison, WI, USA) using the method previously described for SMO/PAOh1 [ ]. .. The resulting pET15b constructs, containing either SMO/PAOh1 or PAOh4, were then combined to produce SMO5/pET15b.

    Modification:

    Article Title: Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31
    Article Snippet: .. The DNA coding fragment for the Bap31 leucine-zipper region was obtained by standard PCR methods using a full-length human Bap31 clone as template (GenBank accession No. ) and subcloned into the Nde I/ Bam HI sites of a modified pET15b bacterial expression vector (Novagen), thus adding an N-­terminal 6×His tag to the protein (6×His-Met-Gly160). ..

    Transformation Assay:

    Article Title: Low Molecular Weight Amidoximes that Act as Potent Inhibitors of Lysine-Specific Demethylase 1
    Article Snippet: .. Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. Following selection, expression and purification of recombinant LSD1 protein were performed as previously described.

    Article Title: (Bis)urea and (Bis)thiourea Inhibitors of Lysine-Specific Demethylase 1 as Epigenetic Modulators
    Article Snippet: .. Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. Following selection, expression and purification of recombinant LSD1 protein were performed as previously described.

    Plasmid Preparation:

    Article Title: Low Molecular Weight Amidoximes that Act as Potent Inhibitors of Lysine-Specific Demethylase 1
    Article Snippet: .. Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. Following selection, expression and purification of recombinant LSD1 protein were performed as previously described.

    Article Title: (Bis)urea and (Bis)thiourea Inhibitors of Lysine-Specific Demethylase 1 as Epigenetic Modulators
    Article Snippet: .. Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. Following selection, expression and purification of recombinant LSD1 protein were performed as previously described.

    Article Title: A Novel Pyridoxal 5?-Phosphate-dependent Amino Acid Racemase in the Aplysia californica Central Nervous System *
    Article Snippet: .. The clone T90 plasmid and pET15b bacterial expression vector (EMD Chemicals) were double digested with NdeI and XhoI restriction enzymes (New England BioLabs, Inc.). .. The dar1 fragment and cleaved vector were gel-purified, ligated, and transformed into TOP10 cells.

    Article Title: Human amnion‐derived mesenchymal stem cells are a potential source for uterine stem cell therapy
    Article Snippet: .. Full‐length enhanced green fluorescence protein (EGFP) was amplified using pEGFP‐N1 vector (Invitrogen) as a template, and cloned into Xho I and Bam H1 sites of the pET15b bacterial expression vector (Novagen, Darmstadt, Germany). .. MPG peptide (GALFLGWLGAAGSTMGAPKKKRKV) sequence ( ) was amplified by Klenow reaction and was cloned into Nde I and Xho I sites in pET15b‐EGFP.

    Article Title: CSN5/JAB1 Interacts with the Centromeric Components CENP-T and CENP-W and Regulates Their Proteasome-mediated Degradation *
    Article Snippet: .. For the in vitro protein-binding assay, CSN5, CENP-W, and CENP-T were first subcloned into the pET15b bacterial expression vector (Novagen). .. Next, the His-tagged recombinant proteins were expressed using the Escherichia coli S30 T7 protein expression system (Promega), and co-immunoprecipitation was carried out using an anti-CSN5 antibody (Santa Cruz).

    Article Title: Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31
    Article Snippet: .. The DNA coding fragment for the Bap31 leucine-zipper region was obtained by standard PCR methods using a full-length human Bap31 clone as template (GenBank accession No. ) and subcloned into the Nde I/ Bam HI sites of a modified pET15b bacterial expression vector (Novagen), thus adding an N-­terminal 6×His tag to the protein (6×His-Met-Gly160). ..

    Article Title: Nuclear localization of human spermine oxidase isoforms - possible implications in drug response and disease etiology
    Article Snippet: .. PAOh4 cDNA was cloned into the pET15b bacterial expression vector (Novagen, Madison, WI, USA) using the method previously described for SMO/PAOh1 [ ]. .. The resulting pET15b constructs, containing either SMO/PAOh1 or PAOh4, were then combined to produce SMO5/pET15b.

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  • 92
    Millipore expression vector pet15b
    Expression Vector Pet15b, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression vector pet15b/product/Millipore
    Average 92 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    expression vector pet15b - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    88
    Millipore pet15b bacterial expression vector
    Pet15b Bacterial Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet15b bacterial expression vector/product/Millipore
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    pet15b bacterial expression vector - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

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