pet15b bacterial expression vector  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 87

    Structured Review

    Millipore pet15b bacterial expression vector
    Pet15b Bacterial Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet15b bacterial expression vector/product/Millipore
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pet15b bacterial expression vector - by Bioz Stars, 2020-04
    87/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Nuclear localization of human spermine oxidase isoforms - possible implications in drug response and disease etiology
    Article Snippet: .. PAOh4 cDNA was cloned into the pET15b bacterial expression vector (Novagen, Madison, WI, USA) using the method previously described for SMO/PAOh1 [ ]. .. The resulting pET15b constructs, containing either SMO/PAOh1 or PAOh4, were then combined to produce SMO5/pET15b.

    Amplification:

    Article Title: CSN5/JAB1 Interacts with the Centromeric Components CENP-T and CENP-W and Regulates Their Proteasome-mediated Degradation *
    Article Snippet: By using EcoRI and SalI, the C terminus region (amino acids 455–546) was amplified and subcloned into the pGBKT7 vector (Clontech) to generate a bait plasmid. .. For the in vitro protein-binding assay, CSN5, CENP-W, and CENP-T were first subcloned into the pET15b bacterial expression vector (Novagen).

    Construct:

    Article Title: Nuclear localization of human spermine oxidase isoforms - possible implications in drug response and disease etiology
    Article Snippet: PAOh4 cDNA was cloned into the pET15b bacterial expression vector (Novagen, Madison, WI, USA) using the method previously described for SMO/PAOh1 [ ]. .. The resulting pET15b constructs, containing either SMO/PAOh1 or PAOh4, were then combined to produce SMO5/pET15b.

    Incubation:

    Article Title: CSN5/JAB1 Interacts with the Centromeric Components CENP-T and CENP-W and Regulates Their Proteasome-mediated Degradation *
    Article Snippet: For glutathione S -transferase (GST) pull-down, the cell lysate was incubated with 20 μl of a 50% slurry suspension of glutathione-agarose beads (Peptron, Korea) for 2 h at 4 °C with gentle rotation. .. For the in vitro protein-binding assay, CSN5, CENP-W, and CENP-T were first subcloned into the pET15b bacterial expression vector (Novagen).

    Expressing:

    Article Title: Low Molecular Weight Amidoximes that Act as Potent Inhibitors of Lysine-Specific Demethylase 1
    Article Snippet: .. Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. Following selection, expression and purification of recombinant LSD1 protein were performed as previously described.

    Article Title: (Bis)urea and (Bis)thiourea Inhibitors of Lysine-Specific Demethylase 1 as Epigenetic Modulators
    Article Snippet: .. Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. Following selection, expression and purification of recombinant LSD1 protein were performed as previously described.

    Article Title: A Novel Pyridoxal 5?-Phosphate-dependent Amino Acid Racemase in the Aplysia californica Central Nervous System *
    Article Snippet: .. The clone T90 plasmid and pET15b bacterial expression vector (EMD Chemicals) were double digested with NdeI and XhoI restriction enzymes (New England BioLabs, Inc.). .. The dar1 fragment and cleaved vector were gel-purified, ligated, and transformed into TOP10 cells.

    Article Title: CSN5/JAB1 Interacts with the Centromeric Components CENP-T and CENP-W and Regulates Their Proteasome-mediated Degradation *
    Article Snippet: .. For the in vitro protein-binding assay, CSN5, CENP-W, and CENP-T were first subcloned into the pET15b bacterial expression vector (Novagen). .. Next, the His-tagged recombinant proteins were expressed using the Escherichia coli S30 T7 protein expression system (Promega), and co-immunoprecipitation was carried out using an anti-CSN5 antibody (Santa Cruz).

    Article Title: Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31
    Article Snippet: .. The DNA coding fragment for the Bap31 leucine-zipper region was obtained by standard PCR methods using a full-length human Bap31 clone as template (GenBank accession No. ) and subcloned into the Nde I/ Bam HI sites of a modified pET15b bacterial expression vector (Novagen), thus adding an N-­terminal 6×His tag to the protein (6×His-Met-Gly160). ..

    Article Title: Nuclear localization of human spermine oxidase isoforms - possible implications in drug response and disease etiology
    Article Snippet: .. PAOh4 cDNA was cloned into the pET15b bacterial expression vector (Novagen, Madison, WI, USA) using the method previously described for SMO/PAOh1 [ ]. .. The resulting pET15b constructs, containing either SMO/PAOh1 or PAOh4, were then combined to produce SMO5/pET15b.

    Modification:

    Article Title: Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31
    Article Snippet: .. The DNA coding fragment for the Bap31 leucine-zipper region was obtained by standard PCR methods using a full-length human Bap31 clone as template (GenBank accession No. ) and subcloned into the Nde I/ Bam HI sites of a modified pET15b bacterial expression vector (Novagen), thus adding an N-­terminal 6×His tag to the protein (6×His-Met-Gly160). ..

    Transformation Assay:

    Article Title: Low Molecular Weight Amidoximes that Act as Potent Inhibitors of Lysine-Specific Demethylase 1
    Article Snippet: .. Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. Following selection, expression and purification of recombinant LSD1 protein were performed as previously described.

    Article Title: (Bis)urea and (Bis)thiourea Inhibitors of Lysine-Specific Demethylase 1 as Epigenetic Modulators
    Article Snippet: .. Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. Following selection, expression and purification of recombinant LSD1 protein were performed as previously described.

    Article Title: A Novel Pyridoxal 5?-Phosphate-dependent Amino Acid Racemase in the Aplysia californica Central Nervous System *
    Article Snippet: The clone T90 plasmid and pET15b bacterial expression vector (EMD Chemicals) were double digested with NdeI and XhoI restriction enzymes (New England BioLabs, Inc.). .. The dar1 fragment and cleaved vector were gel-purified, ligated, and transformed into TOP10 cells.

    Article Title: Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31
    Article Snippet: The DNA coding fragment for the Bap31 leucine-zipper region was obtained by standard PCR methods using a full-length human Bap31 clone as template (GenBank accession No. ) and subcloned into the Nde I/ Bam HI sites of a modified pET15b bacterial expression vector (Novagen), thus adding an N-­terminal 6×His tag to the protein (6×His-Met-Gly160). .. The ligated plasmid was transformed into Escherichia coli strain BL21(DE3).

    Over Expression:

    Article Title: Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31
    Article Snippet: Paragraph title: 2.1. Subcloning, overexpression and protein purification ... The DNA coding fragment for the Bap31 leucine-zipper region was obtained by standard PCR methods using a full-length human Bap31 clone as template (GenBank accession No. ) and subcloned into the Nde I/ Bam HI sites of a modified pET15b bacterial expression vector (Novagen), thus adding an N-­terminal 6×His tag to the protein (6×His-Met-Gly160).

    Protease Inhibitor:

    Article Title: CSN5/JAB1 Interacts with the Centromeric Components CENP-T and CENP-W and Regulates Their Proteasome-mediated Degradation *
    Article Snippet: For co-immunoprecipitation, cells were first lysed in a lysis buffer (50 m m Tris, pH 7.5, 150 m m NaCl, 0.5% Nonidet P-40, 0.5% Triton X-100) supplemented with Protease Inhibitor Mixture (Sigma). .. For the in vitro protein-binding assay, CSN5, CENP-W, and CENP-T were first subcloned into the pET15b bacterial expression vector (Novagen).

    Sequencing:

    Article Title: A Novel Pyridoxal 5?-Phosphate-dependent Amino Acid Racemase in the Aplysia californica Central Nervous System *
    Article Snippet: The clone T90 plasmid and pET15b bacterial expression vector (EMD Chemicals) were double digested with NdeI and XhoI restriction enzymes (New England BioLabs, Inc.). .. A sequence-confirmed clone was transformed into BL21 (DE3) bacterial cells (EMD Chemicals) to generate the bacterial clone 15bT90-31.

    Affinity Purification:

    Article Title: Low Molecular Weight Amidoximes that Act as Potent Inhibitors of Lysine-Specific Demethylase 1
    Article Snippet: Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. The HIS-tagged protein was purified using Ni-NTA affinity purification resin and column as recommended by the manufacturer (Qiagen, Valencia, California).

    Article Title: (Bis)urea and (Bis)thiourea Inhibitors of Lysine-Specific Demethylase 1 as Epigenetic Modulators
    Article Snippet: Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. The HIS-tagged protein was purified using Ni-NTA affinity purification resin and column as recommended by the manufacturer (Qiagen, Valencia, California).

    Recombinant:

    Article Title: Low Molecular Weight Amidoximes that Act as Potent Inhibitors of Lysine-Specific Demethylase 1
    Article Snippet: Paragraph title: Expression and Purification of Recombinant Proteins and the Histone Demethylase Assay ... Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli .

    Article Title: (Bis)urea and (Bis)thiourea Inhibitors of Lysine-Specific Demethylase 1 as Epigenetic Modulators
    Article Snippet: Paragraph title: Expression, Purification and Demethylase Assay of Recombinant Proteins ... Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli .

    Article Title: CSN5/JAB1 Interacts with the Centromeric Components CENP-T and CENP-W and Regulates Their Proteasome-mediated Degradation *
    Article Snippet: For the in vitro protein-binding assay, CSN5, CENP-W, and CENP-T were first subcloned into the pET15b bacterial expression vector (Novagen). .. Next, the His-tagged recombinant proteins were expressed using the Escherichia coli S30 T7 protein expression system (Promega), and co-immunoprecipitation was carried out using an anti-CSN5 antibody (Santa Cruz).

    Article Title: Nuclear localization of human spermine oxidase isoforms - possible implications in drug response and disease etiology
    Article Snippet: Paragraph title: Construction and purification of recombinant SMO5/pET15b ... PAOh4 cDNA was cloned into the pET15b bacterial expression vector (Novagen, Madison, WI, USA) using the method previously described for SMO/PAOh1 [ ].

    Isolation:

    Article Title: Nuclear localization of human spermine oxidase isoforms - possible implications in drug response and disease etiology
    Article Snippet: PAOh4 cDNA was cloned into the pET15b bacterial expression vector (Novagen, Madison, WI, USA) using the method previously described for SMO/PAOh1 [ ]. .. Specifically, the Xba I to Kpn I fragment of SMO/PAOh1 in pET15b was isolated and ligated to the purified Kpn I to Xba I fragment of PAOh4 in pET15b.

    Subcloning:

    Article Title: Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31
    Article Snippet: Paragraph title: 2.1. Subcloning, overexpression and protein purification ... The DNA coding fragment for the Bap31 leucine-zipper region was obtained by standard PCR methods using a full-length human Bap31 clone as template (GenBank accession No. ) and subcloned into the Nde I/ Bam HI sites of a modified pET15b bacterial expression vector (Novagen), thus adding an N-­terminal 6×His tag to the protein (6×His-Met-Gly160).

    Purification:

    Article Title: Low Molecular Weight Amidoximes that Act as Potent Inhibitors of Lysine-Specific Demethylase 1
    Article Snippet: Paragraph title: Expression and Purification of Recombinant Proteins and the Histone Demethylase Assay ... Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli .

    Article Title: (Bis)urea and (Bis)thiourea Inhibitors of Lysine-Specific Demethylase 1 as Epigenetic Modulators
    Article Snippet: Paragraph title: Expression, Purification and Demethylase Assay of Recombinant Proteins ... Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli .

    Article Title: Nuclear localization of human spermine oxidase isoforms - possible implications in drug response and disease etiology
    Article Snippet: Paragraph title: Construction and purification of recombinant SMO5/pET15b ... PAOh4 cDNA was cloned into the pET15b bacterial expression vector (Novagen, Madison, WI, USA) using the method previously described for SMO/PAOh1 [ ].

    Protein Purification:

    Article Title: Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31
    Article Snippet: Paragraph title: 2.1. Subcloning, overexpression and protein purification ... The DNA coding fragment for the Bap31 leucine-zipper region was obtained by standard PCR methods using a full-length human Bap31 clone as template (GenBank accession No. ) and subcloned into the Nde I/ Bam HI sites of a modified pET15b bacterial expression vector (Novagen), thus adding an N-­terminal 6×His tag to the protein (6×His-Met-Gly160).

    Polymerase Chain Reaction:

    Article Title: Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31
    Article Snippet: .. The DNA coding fragment for the Bap31 leucine-zipper region was obtained by standard PCR methods using a full-length human Bap31 clone as template (GenBank accession No. ) and subcloned into the Nde I/ Bam HI sites of a modified pET15b bacterial expression vector (Novagen), thus adding an N-­terminal 6×His tag to the protein (6×His-Met-Gly160). ..

    Plasmid Preparation:

    Article Title: Low Molecular Weight Amidoximes that Act as Potent Inhibitors of Lysine-Specific Demethylase 1
    Article Snippet: .. Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. Following selection, expression and purification of recombinant LSD1 protein were performed as previously described.

    Article Title: (Bis)urea and (Bis)thiourea Inhibitors of Lysine-Specific Demethylase 1 as Epigenetic Modulators
    Article Snippet: .. Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. Following selection, expression and purification of recombinant LSD1 protein were performed as previously described.

    Article Title: A Novel Pyridoxal 5?-Phosphate-dependent Amino Acid Racemase in the Aplysia californica Central Nervous System *
    Article Snippet: .. The clone T90 plasmid and pET15b bacterial expression vector (EMD Chemicals) were double digested with NdeI and XhoI restriction enzymes (New England BioLabs, Inc.). .. The dar1 fragment and cleaved vector were gel-purified, ligated, and transformed into TOP10 cells.

    Article Title: CSN5/JAB1 Interacts with the Centromeric Components CENP-T and CENP-W and Regulates Their Proteasome-mediated Degradation *
    Article Snippet: .. For the in vitro protein-binding assay, CSN5, CENP-W, and CENP-T were first subcloned into the pET15b bacterial expression vector (Novagen). .. Next, the His-tagged recombinant proteins were expressed using the Escherichia coli S30 T7 protein expression system (Promega), and co-immunoprecipitation was carried out using an anti-CSN5 antibody (Santa Cruz).

    Article Title: Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31
    Article Snippet: .. The DNA coding fragment for the Bap31 leucine-zipper region was obtained by standard PCR methods using a full-length human Bap31 clone as template (GenBank accession No. ) and subcloned into the Nde I/ Bam HI sites of a modified pET15b bacterial expression vector (Novagen), thus adding an N-­terminal 6×His tag to the protein (6×His-Met-Gly160). ..

    Article Title: Nuclear localization of human spermine oxidase isoforms - possible implications in drug response and disease etiology
    Article Snippet: .. PAOh4 cDNA was cloned into the pET15b bacterial expression vector (Novagen, Madison, WI, USA) using the method previously described for SMO/PAOh1 [ ]. .. The resulting pET15b constructs, containing either SMO/PAOh1 or PAOh4, were then combined to produce SMO5/pET15b.

    Software:

    Article Title: Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31
    Article Snippet: Using bioinformatics software, the leucine-zipper region was predicted to run from Leu168 to Leu233 (Przybylski & Rost, 2002 ). .. The DNA coding fragment for the Bap31 leucine-zipper region was obtained by standard PCR methods using a full-length human Bap31 clone as template (GenBank accession No. ) and subcloned into the Nde I/ Bam HI sites of a modified pET15b bacterial expression vector (Novagen), thus adding an N-­terminal 6×His tag to the protein (6×His-Met-Gly160).

    Selection:

    Article Title: Low Molecular Weight Amidoximes that Act as Potent Inhibitors of Lysine-Specific Demethylase 1
    Article Snippet: Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. Following selection, expression and purification of recombinant LSD1 protein were performed as previously described.

    Article Title: (Bis)urea and (Bis)thiourea Inhibitors of Lysine-Specific Demethylase 1 as Epigenetic Modulators
    Article Snippet: Full-length human LSD1 cDNA was subcloned into the pET15b bacterial expression vector (Novagen, Madison, WI) in frame with an N-terminal 6× HIS-tag and transformed into the BL21 (DE3 ) strain of Escherichia coli . .. Following selection, expression and purification of recombinant LSD1 protein were performed as previously described.

    Two Hybrid Screening:

    Article Title: CSN5/JAB1 Interacts with the Centromeric Components CENP-T and CENP-W and Regulates Their Proteasome-mediated Degradation *
    Article Snippet: Paragraph title: Yeast Two-hybrid Screening, Protein Binding, and Antibodies ... For the in vitro protein-binding assay, CSN5, CENP-W, and CENP-T were first subcloned into the pET15b bacterial expression vector (Novagen).

    In Vitro:

    Article Title: CSN5/JAB1 Interacts with the Centromeric Components CENP-T and CENP-W and Regulates Their Proteasome-mediated Degradation *
    Article Snippet: .. For the in vitro protein-binding assay, CSN5, CENP-W, and CENP-T were first subcloned into the pET15b bacterial expression vector (Novagen). .. Next, the His-tagged recombinant proteins were expressed using the Escherichia coli S30 T7 protein expression system (Promega), and co-immunoprecipitation was carried out using an anti-CSN5 antibody (Santa Cruz).

    Protein Binding:

    Article Title: CSN5/JAB1 Interacts with the Centromeric Components CENP-T and CENP-W and Regulates Their Proteasome-mediated Degradation *
    Article Snippet: .. For the in vitro protein-binding assay, CSN5, CENP-W, and CENP-T were first subcloned into the pET15b bacterial expression vector (Novagen). .. Next, the His-tagged recombinant proteins were expressed using the Escherichia coli S30 T7 protein expression system (Promega), and co-immunoprecipitation was carried out using an anti-CSN5 antibody (Santa Cruz).

    Lysis:

    Article Title: CSN5/JAB1 Interacts with the Centromeric Components CENP-T and CENP-W and Regulates Their Proteasome-mediated Degradation *
    Article Snippet: For co-immunoprecipitation, cells were first lysed in a lysis buffer (50 m m Tris, pH 7.5, 150 m m NaCl, 0.5% Nonidet P-40, 0.5% Triton X-100) supplemented with Protease Inhibitor Mixture (Sigma). .. For the in vitro protein-binding assay, CSN5, CENP-W, and CENP-T were first subcloned into the pET15b bacterial expression vector (Novagen).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Millipore expression vector pet15b
    Expression Vector Pet15b, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression vector pet15b/product/Millipore
    Average 92 stars, based on 110 article reviews
    Price from $9.99 to $1999.99
    expression vector pet15b - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    87
    Millipore pet15b bacterial expression vector
    Pet15b Bacterial Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet15b bacterial expression vector/product/Millipore
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pet15b bacterial expression vector - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    Image Search Results