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    Thermo Fisher pet fluorescent dyes
    Pet Fluorescent Dyes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet fluorescent dyes/product/Thermo Fisher
    Average 86 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    pet fluorescent dyes - by Bioz Stars, 2020-01
    86/100 stars

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    Multiplexing:

    Article Title: Sequencing and Characterization of Striped Venus Transcriptome Expand Resources for Clam Fishery Genetics
    Article Snippet: FASTPCR was also used to test for primer pairs compatibility to avoid primer dimers, self-annealing and hairpin formation when multiplexing loci during PCR. .. Forward primers were labelled with FAM, VIC, NED and PET fluorescent dyes (Applied Biosystems) to verify the electrophoresis-predicted polymorphism.

    DNA Extraction:

    Article Title: Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera. Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera
    Article Snippet: 2.6 Genetic differentiation between sites DNA was extracted from wild collected fish (Table for sample sizes) using the Wizard® DNA extraction kit (Promega Corporation, Madison, WI, USA). .. Forward primers were labeled using 6‐FAM, NED, VIC, PET® fluorescent dyes (Applied Biosystems, Inc., Foster City, CA, USA).

    Multiplex Assay:

    Article Title: Characterization and multiplexing of 21 microsatellite markers for the herb Noccaea caerulescens (Brassicaceae) 1
    Article Snippet: The PCR reactions were carried out in a total volume of 10 μL, containing 1 μL of DNA template, forward and reverse primers (0.2 μM), and 1× QIAGEN Multiplex PCR Master Mix (QIAGEN, Courtaboeuf, France). .. Forward primers were labeled with one of the FAM, NED, VIC, or PET fluorescent dyes (Applied Biosystems, Waltham, Massachusetts, USA).

    Article Title: Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera. Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera
    Article Snippet: Forward primers were labeled using 6‐FAM, NED, VIC, PET® fluorescent dyes (Applied Biosystems, Inc., Foster City, CA, USA). .. All loci were amplified in the same multiplex reaction using the Qiagen multiplex PCR kit (Qiagen, Venlo, The Netherlands).

    Article Title: Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification
    Article Snippet: .. After conjugating 6-FAM, HEX, NED or PET fluorescent dyes (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) to the 5′-end of each forward primer, we performed multiplex PCR for the seven selected primer sets using a Qiagen multiplex PCR kit (Qiagen, Limburg, The Netherlands). .. The 10-μL reaction mixture contained 1× Qiagen multiplex PCR master mix, 0.2 μM of each primer, 2 μL of distilled water, and 1 μL of DNA solution.

    Positron Emission Tomography:

    Article Title: A major effect quantitative trait locus for whirling disease resistance identified in rainbow trout (Oncorhynchus mykiss)
    Article Snippet: .. For the F2 mapping families, 3–12 microsatellite loci were multiplexed in each reaction using M13, SP6, T7P or T7T universal primer tails ( ) labeled with 6-FAM, VIC, NED or PET fluorescent dyes (Applied Biosystems). .. PCR was performed in a 10 μl total volume containing: 2 μl extracted genomic DNA, 1 × PCR reaction buffer (Roche, Indianapolis, IN, USA), 2.0 m MgCl2 , 0.2 m of each dNTP, 1 μ of each reverse primer, 0.8 μ of each forward primer, 0.3–0.7 μ of each universal fluorescent tailed primer, and 1.0 U of FastStart Taq DNA polymerase (Roche).

    Article Title: Characterization and multiplexing of 21 microsatellite markers for the herb Noccaea caerulescens (Brassicaceae) 1
    Article Snippet: .. Forward primers were labeled with one of the FAM, NED, VIC, or PET fluorescent dyes (Applied Biosystems, Waltham, Massachusetts, USA). .. PCR products were analyzed separately through electrophoresis on an ABI3130 Genetic Analyzer (Applied Biosystems).

    Article Title: Sequencing and Characterization of Striped Venus Transcriptome Expand Resources for Clam Fishery Genetics
    Article Snippet: .. Forward primers were labelled with FAM, VIC, NED and PET fluorescent dyes (Applied Biosystems) to verify the electrophoresis-predicted polymorphism. .. A fraction of the PCR product was loaded on an Applied Biosystems 3130 XL automated sequencer (Liz500 as size standard, genotyping facility at www.bmr-genomics.com ) and allele sizes were assigned using GENEMARKER 1.71 (SoftGenetics, State College, Pennsylvania).

    Article Title: Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera. Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera
    Article Snippet: .. Forward primers were labeled using 6‐FAM, NED, VIC, PET® fluorescent dyes (Applied Biosystems, Inc., Foster City, CA, USA). .. All loci were amplified in the same multiplex reaction using the Qiagen multiplex PCR kit (Qiagen, Venlo, The Netherlands).

    Article Title: Characterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross-amplification in 10 other subgenera 1
    Article Snippet: .. Following the procedure developed by , the sequence-specific forward primers were marked at the 5′ end with an M13 tail (5′-TGTAAAACGACGGCCAGT-3′) (Eurofins), which was then labeled with 5-FAM, VIC, NED, or PET fluorescent dyes ( ) (Life Technologies). .. The PCR mix contained 1× PCR Green GoTaq (Promega Corporation), 0.2 mM of each dNTP, 0.16 mM of each reverse and fluorescent-labeled M13 primer, 0.04 mM of forward primer, 0.75 units GoTaq DNA Polymerase, and 50 ng of DNA template in a total volume of 15 μL.

    Article Title: Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification
    Article Snippet: .. After conjugating 6-FAM, HEX, NED or PET fluorescent dyes (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) to the 5′-end of each forward primer, we performed multiplex PCR for the seven selected primer sets using a Qiagen multiplex PCR kit (Qiagen, Limburg, The Netherlands). .. The 10-μL reaction mixture contained 1× Qiagen multiplex PCR master mix, 0.2 μM of each primer, 2 μL of distilled water, and 1 μL of DNA solution.

    Agarose Gel Electrophoresis:

    Article Title: Characterization and multiplexing of 21 microsatellite markers for the herb Noccaea caerulescens (Brassicaceae) 1
    Article Snippet: Amplification products were visualized with agarose gel (2%) with ethidium bromide stain. .. Forward primers were labeled with one of the FAM, NED, VIC, or PET fluorescent dyes (Applied Biosystems, Waltham, Massachusetts, USA).

    Article Title: Characterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross-amplification in 10 other subgenera 1
    Article Snippet: Following the procedure developed by , the sequence-specific forward primers were marked at the 5′ end with an M13 tail (5′-TGTAAAACGACGGCCAGT-3′) (Eurofins), which was then labeled with 5-FAM, VIC, NED, or PET fluorescent dyes ( ) (Life Technologies). .. Following the procedure developed by , the sequence-specific forward primers were marked at the 5′ end with an M13 tail (5′-TGTAAAACGACGGCCAGT-3′) (Eurofins), which was then labeled with 5-FAM, VIC, NED, or PET fluorescent dyes ( ) (Life Technologies).

    In Vitro:

    Article Title: Sequencing and Characterization of Striped Venus Transcriptome Expand Resources for Clam Fishery Genetics
    Article Snippet: Paragraph title: SSRs detection and in vitro validation ... Forward primers were labelled with FAM, VIC, NED and PET fluorescent dyes (Applied Biosystems) to verify the electrophoresis-predicted polymorphism.

    Electrophoresis:

    Article Title: A major effect quantitative trait locus for whirling disease resistance identified in rainbow trout (Oncorhynchus mykiss)
    Article Snippet: For the F2 mapping families, 3–12 microsatellite loci were multiplexed in each reaction using M13, SP6, T7P or T7T universal primer tails ( ) labeled with 6-FAM, VIC, NED or PET fluorescent dyes (Applied Biosystems). .. Thermal cycler conditions were as follows: 95 °C for 5 min followed by five cycles of 94 °C for 45 sec, 68 °C (−2 °C per cycle) for 5 min, 72 °C for 1 min followed by five cycles of 94 °C for 45 sec, 58 °C (−2 °C per cycle) for 2 min, 72 °C for 1 min followed by 30 cycles of 94 °C for 45 sec, 50 °C for 2 min, 72 °C for 1 min and ending with a final extension of 72 °C for 2 min. PCR products were visualized by capillary electrophoresis using a 3730xl DNA Analyzer (Applied Biosystems) and allele sizes were determined using GeneMapper software (Applied Biosystems) with internal GeneScan 600 LIZ size standards (Applied Biosystems).

    Article Title: Characterization and multiplexing of 21 microsatellite markers for the herb Noccaea caerulescens (Brassicaceae) 1
    Article Snippet: Forward primers were labeled with one of the FAM, NED, VIC, or PET fluorescent dyes (Applied Biosystems, Waltham, Massachusetts, USA). .. PCR products were analyzed separately through electrophoresis on an ABI3130 Genetic Analyzer (Applied Biosystems).

    Article Title: Sequencing and Characterization of Striped Venus Transcriptome Expand Resources for Clam Fishery Genetics
    Article Snippet: .. Forward primers were labelled with FAM, VIC, NED and PET fluorescent dyes (Applied Biosystems) to verify the electrophoresis-predicted polymorphism. .. A fraction of the PCR product was loaded on an Applied Biosystems 3130 XL automated sequencer (Liz500 as size standard, genotyping facility at www.bmr-genomics.com ) and allele sizes were assigned using GENEMARKER 1.71 (SoftGenetics, State College, Pennsylvania).

    Article Title: Characterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross-amplification in 10 other subgenera 1
    Article Snippet: Following the procedure developed by , the sequence-specific forward primers were marked at the 5′ end with an M13 tail (5′-TGTAAAACGACGGCCAGT-3′) (Eurofins), which was then labeled with 5-FAM, VIC, NED, or PET fluorescent dyes ( ) (Life Technologies). .. Following the procedure developed by , the sequence-specific forward primers were marked at the 5′ end with an M13 tail (5′-TGTAAAACGACGGCCAGT-3′) (Eurofins), which was then labeled with 5-FAM, VIC, NED, or PET fluorescent dyes ( ) (Life Technologies).

    Northern Blot:

    Article Title: Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification
    Article Snippet: PCR Amplification and Genotyping Three wild population samples (O. m. masou from the Chitose River, Hokkaido Island, Japan; O. m. ishikawae from the Miya River, central Honshu, Japan; and O. m. subsp. from Lake Biwa, central Honshu, Japan) and one hatchery-reared stock sample (O. m. masou from the Ohara River, northern Honshu, Japan) were used for polymorphism detection. .. After conjugating 6-FAM, HEX, NED or PET fluorescent dyes (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) to the 5′-end of each forward primer, we performed multiplex PCR for the seven selected primer sets using a Qiagen multiplex PCR kit (Qiagen, Limburg, The Netherlands).

    Size-exclusion Chromatography:

    Article Title: A major effect quantitative trait locus for whirling disease resistance identified in rainbow trout (Oncorhynchus mykiss)
    Article Snippet: For the F2 mapping families, 3–12 microsatellite loci were multiplexed in each reaction using M13, SP6, T7P or T7T universal primer tails ( ) labeled with 6-FAM, VIC, NED or PET fluorescent dyes (Applied Biosystems). .. Thermal cycler conditions were as follows: 95 °C for 5 min followed by five cycles of 94 °C for 45 sec, 68 °C (−2 °C per cycle) for 5 min, 72 °C for 1 min followed by five cycles of 94 °C for 45 sec, 58 °C (−2 °C per cycle) for 2 min, 72 °C for 1 min followed by 30 cycles of 94 °C for 45 sec, 50 °C for 2 min, 72 °C for 1 min and ending with a final extension of 72 °C for 2 min. PCR products were visualized by capillary electrophoresis using a 3730xl DNA Analyzer (Applied Biosystems) and allele sizes were determined using GeneMapper software (Applied Biosystems) with internal GeneScan 600 LIZ size standards (Applied Biosystems).

    Article Title: Sequencing and Characterization of Striped Venus Transcriptome Expand Resources for Clam Fishery Genetics
    Article Snippet: PCR conditions were: initial denaturation at 94°C for 1 min., followed by 30 cycles of 94°C for 30 sec. (denaturation), 54–58°C for 30 sec. (annealing) (see for detailed annealing temperature for each locus), 72°C for 30 sec. (extension) and a final single extension step at 72°C for 5 min. Electrophoresis was carried out at 100 V on 3% agarose TAE gels supplemented with 0.2 µg/ml of Gel Red (Biotium Inc.) for a preliminary polymorphism detection. .. Forward primers were labelled with FAM, VIC, NED and PET fluorescent dyes (Applied Biosystems) to verify the electrophoresis-predicted polymorphism.

    Labeling:

    Article Title: A major effect quantitative trait locus for whirling disease resistance identified in rainbow trout (Oncorhynchus mykiss)
    Article Snippet: .. For the F2 mapping families, 3–12 microsatellite loci were multiplexed in each reaction using M13, SP6, T7P or T7T universal primer tails ( ) labeled with 6-FAM, VIC, NED or PET fluorescent dyes (Applied Biosystems). .. PCR was performed in a 10 μl total volume containing: 2 μl extracted genomic DNA, 1 × PCR reaction buffer (Roche, Indianapolis, IN, USA), 2.0 m MgCl2 , 0.2 m of each dNTP, 1 μ of each reverse primer, 0.8 μ of each forward primer, 0.3–0.7 μ of each universal fluorescent tailed primer, and 1.0 U of FastStart Taq DNA polymerase (Roche).

    Article Title: Characterization and multiplexing of 21 microsatellite markers for the herb Noccaea caerulescens (Brassicaceae) 1
    Article Snippet: .. Forward primers were labeled with one of the FAM, NED, VIC, or PET fluorescent dyes (Applied Biosystems, Waltham, Massachusetts, USA). .. PCR products were analyzed separately through electrophoresis on an ABI3130 Genetic Analyzer (Applied Biosystems).

    Article Title: Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera. Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera
    Article Snippet: .. Forward primers were labeled using 6‐FAM, NED, VIC, PET® fluorescent dyes (Applied Biosystems, Inc., Foster City, CA, USA). .. All loci were amplified in the same multiplex reaction using the Qiagen multiplex PCR kit (Qiagen, Venlo, The Netherlands).

    Article Title: Characterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross-amplification in 10 other subgenera 1
    Article Snippet: .. Following the procedure developed by , the sequence-specific forward primers were marked at the 5′ end with an M13 tail (5′-TGTAAAACGACGGCCAGT-3′) (Eurofins), which was then labeled with 5-FAM, VIC, NED, or PET fluorescent dyes ( ) (Life Technologies). .. The PCR mix contained 1× PCR Green GoTaq (Promega Corporation), 0.2 mM of each dNTP, 0.16 mM of each reverse and fluorescent-labeled M13 primer, 0.04 mM of forward primer, 0.75 units GoTaq DNA Polymerase, and 50 ng of DNA template in a total volume of 15 μL.

    Produced:

    Article Title: Sequencing and Characterization of Striped Venus Transcriptome Expand Resources for Clam Fishery Genetics
    Article Snippet: Forward primers were labelled with FAM, VIC, NED and PET fluorescent dyes (Applied Biosystems) to verify the electrophoresis-predicted polymorphism. .. Binning was automated with FLEXIBIN and all input files for further analysis were produced with CREATE 1.33 .

    Polymerase Chain Reaction:

    Article Title: A major effect quantitative trait locus for whirling disease resistance identified in rainbow trout (Oncorhynchus mykiss)
    Article Snippet: For the F2 mapping families, 3–12 microsatellite loci were multiplexed in each reaction using M13, SP6, T7P or T7T universal primer tails ( ) labeled with 6-FAM, VIC, NED or PET fluorescent dyes (Applied Biosystems). .. PCR was performed in a 10 μl total volume containing: 2 μl extracted genomic DNA, 1 × PCR reaction buffer (Roche, Indianapolis, IN, USA), 2.0 m MgCl2 , 0.2 m of each dNTP, 1 μ of each reverse primer, 0.8 μ of each forward primer, 0.3–0.7 μ of each universal fluorescent tailed primer, and 1.0 U of FastStart Taq DNA polymerase (Roche).

    Article Title: Characterization and multiplexing of 21 microsatellite markers for the herb Noccaea caerulescens (Brassicaceae) 1
    Article Snippet: The PCR reactions were carried out in a total volume of 10 μL, containing 1 μL of DNA template, forward and reverse primers (0.2 μM), and 1× QIAGEN Multiplex PCR Master Mix (QIAGEN, Courtaboeuf, France). .. Forward primers were labeled with one of the FAM, NED, VIC, or PET fluorescent dyes (Applied Biosystems, Waltham, Massachusetts, USA).

    Article Title: Sequencing and Characterization of Striped Venus Transcriptome Expand Resources for Clam Fishery Genetics
    Article Snippet: PCR conditions were: initial denaturation at 94°C for 1 min., followed by 30 cycles of 94°C for 30 sec. (denaturation), 54–58°C for 30 sec. (annealing) (see for detailed annealing temperature for each locus), 72°C for 30 sec. (extension) and a final single extension step at 72°C for 5 min. Electrophoresis was carried out at 100 V on 3% agarose TAE gels supplemented with 0.2 µg/ml of Gel Red (Biotium Inc.) for a preliminary polymorphism detection. .. Forward primers were labelled with FAM, VIC, NED and PET fluorescent dyes (Applied Biosystems) to verify the electrophoresis-predicted polymorphism.

    Article Title: Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera. Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera
    Article Snippet: Forward primers were labeled using 6‐FAM, NED, VIC, PET® fluorescent dyes (Applied Biosystems, Inc., Foster City, CA, USA). .. All loci were amplified in the same multiplex reaction using the Qiagen multiplex PCR kit (Qiagen, Venlo, The Netherlands).

    Article Title: Characterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross-amplification in 10 other subgenera 1
    Article Snippet: [North African clade]), using the same PCR conditions. .. Following the procedure developed by , the sequence-specific forward primers were marked at the 5′ end with an M13 tail (5′-TGTAAAACGACGGCCAGT-3′) (Eurofins), which was then labeled with 5-FAM, VIC, NED, or PET fluorescent dyes ( ) (Life Technologies).

    Article Title: Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification
    Article Snippet: .. After conjugating 6-FAM, HEX, NED or PET fluorescent dyes (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) to the 5′-end of each forward primer, we performed multiplex PCR for the seven selected primer sets using a Qiagen multiplex PCR kit (Qiagen, Limburg, The Netherlands). .. The 10-μL reaction mixture contained 1× Qiagen multiplex PCR master mix, 0.2 μM of each primer, 2 μL of distilled water, and 1 μL of DNA solution.

    Amplification:

    Article Title: Characterization and multiplexing of 21 microsatellite markers for the herb Noccaea caerulescens (Brassicaceae) 1
    Article Snippet: Due to inadequate amplification yield, low specificity, or unexpected size, only 15 markers were kept. .. Forward primers were labeled with one of the FAM, NED, VIC, or PET fluorescent dyes (Applied Biosystems, Waltham, Massachusetts, USA).

    Article Title: Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera. Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera
    Article Snippet: Forward primers were labeled using 6‐FAM, NED, VIC, PET® fluorescent dyes (Applied Biosystems, Inc., Foster City, CA, USA). .. All loci were amplified in the same multiplex reaction using the Qiagen multiplex PCR kit (Qiagen, Venlo, The Netherlands).

    Article Title: Characterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross-amplification in 10 other subgenera 1
    Article Snippet: Following the procedure developed by , the sequence-specific forward primers were marked at the 5′ end with an M13 tail (5′-TGTAAAACGACGGCCAGT-3′) (Eurofins), which was then labeled with 5-FAM, VIC, NED, or PET fluorescent dyes ( ) (Life Technologies). .. Conditions of the PCR amplification were as described above, adding 10 cycles of 1 min at 94°C, 1 min at 53°C, and 50 s at 72°C before the final extension.

    Article Title: Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification
    Article Snippet: Paragraph title: PCR Amplification and Genotyping ... After conjugating 6-FAM, HEX, NED or PET fluorescent dyes (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) to the 5′-end of each forward primer, we performed multiplex PCR for the seven selected primer sets using a Qiagen multiplex PCR kit (Qiagen, Limburg, The Netherlands).

    Modification:

    Article Title: A major effect quantitative trait locus for whirling disease resistance identified in rainbow trout (Oncorhynchus mykiss)
    Article Snippet: The run module was modified with a 3 kV injection for 7 s and fragments were scored using GeneMapper software (Applied Biosystems). .. For the F2 mapping families, 3–12 microsatellite loci were multiplexed in each reaction using M13, SP6, T7P or T7T universal primer tails ( ) labeled with 6-FAM, VIC, NED or PET fluorescent dyes (Applied Biosystems).

    Sequencing:

    Article Title: Characterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross-amplification in 10 other subgenera 1
    Article Snippet: .. Following the procedure developed by , the sequence-specific forward primers were marked at the 5′ end with an M13 tail (5′-TGTAAAACGACGGCCAGT-3′) (Eurofins), which was then labeled with 5-FAM, VIC, NED, or PET fluorescent dyes ( ) (Life Technologies). .. The PCR mix contained 1× PCR Green GoTaq (Promega Corporation), 0.2 mM of each dNTP, 0.16 mM of each reverse and fluorescent-labeled M13 primer, 0.04 mM of forward primer, 0.75 units GoTaq DNA Polymerase, and 50 ng of DNA template in a total volume of 15 μL.

    Staining:

    Article Title: Characterization and multiplexing of 21 microsatellite markers for the herb Noccaea caerulescens (Brassicaceae) 1
    Article Snippet: Amplification products were visualized with agarose gel (2%) with ethidium bromide stain. .. Forward primers were labeled with one of the FAM, NED, VIC, or PET fluorescent dyes (Applied Biosystems, Waltham, Massachusetts, USA).

    Injection:

    Article Title: A major effect quantitative trait locus for whirling disease resistance identified in rainbow trout (Oncorhynchus mykiss)
    Article Snippet: The run module was modified with a 3 kV injection for 7 s and fragments were scored using GeneMapper software (Applied Biosystems). .. For the F2 mapping families, 3–12 microsatellite loci were multiplexed in each reaction using M13, SP6, T7P or T7T universal primer tails ( ) labeled with 6-FAM, VIC, NED or PET fluorescent dyes (Applied Biosystems).

    Fluorescence In Situ Hybridization:

    Article Title: Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera. Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera
    Article Snippet: 2.6 Genetic differentiation between sites DNA was extracted from wild collected fish (Table for sample sizes) using the Wizard® DNA extraction kit (Promega Corporation, Madison, WI, USA). .. Forward primers were labeled using 6‐FAM, NED, VIC, PET® fluorescent dyes (Applied Biosystems, Inc., Foster City, CA, USA).

    Software:

    Article Title: A major effect quantitative trait locus for whirling disease resistance identified in rainbow trout (Oncorhynchus mykiss)
    Article Snippet: The run module was modified with a 3 kV injection for 7 s and fragments were scored using GeneMapper software (Applied Biosystems). .. For the F2 mapping families, 3–12 microsatellite loci were multiplexed in each reaction using M13, SP6, T7P or T7T universal primer tails ( ) labeled with 6-FAM, VIC, NED or PET fluorescent dyes (Applied Biosystems).

    Article Title: Characterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross-amplification in 10 other subgenera 1
    Article Snippet: Following the procedure developed by , the sequence-specific forward primers were marked at the 5′ end with an M13 tail (5′-TGTAAAACGACGGCCAGT-3′) (Eurofins), which was then labeled with 5-FAM, VIC, NED, or PET fluorescent dyes ( ) (Life Technologies). .. PCR products were analyzed with GeneMarker AFLP/Genotyping Software version 1.8 (SoftGenetics, State College, Pennsylvania, USA).

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  • 87
    Thermo Fisher ned fluorescent dyes
    Ned Fluorescent Dyes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ned fluorescent dyes/product/Thermo Fisher
    Average 87 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ned fluorescent dyes - by Bioz Stars, 2020-01
    87/100 stars
      Buy from Supplier

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