pet fluorescent dyes  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pet fluorescent dyes
    Pet Fluorescent Dyes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet fluorescent dyes/product/Thermo Fisher
    Average 88 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    pet fluorescent dyes - by Bioz Stars, 2020-09
    88/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification
    Article Snippet: .. After conjugating 6-FAM, HEX, NED or PET fluorescent dyes (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) to the 5′-end of each forward primer, we performed multiplex PCR for the seven selected primer sets using a Qiagen multiplex PCR kit (Qiagen, Limburg, The Netherlands). .. The 10-μL reaction mixture contained 1× Qiagen multiplex PCR master mix, 0.2 μM of each primer, 2 μL of distilled water, and 1 μL of DNA solution.

    Positron Emission Tomography:

    Article Title: Sequencing and Characterization of Striped Venus Transcriptome Expand Resources for Clam Fishery Genetics
    Article Snippet: .. Forward primers were labelled with FAM, VIC, NED and PET fluorescent dyes (Applied Biosystems) to verify the electrophoresis-predicted polymorphism. .. A fraction of the PCR product was loaded on an Applied Biosystems 3130 XL automated sequencer (Liz500 as size standard, genotyping facility at www.bmr-genomics.com ) and allele sizes were assigned using GENEMARKER 1.71 (SoftGenetics, State College, Pennsylvania).

    Article Title: Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera. Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera
    Article Snippet: .. Forward primers were labeled using 6‐FAM, NED, VIC, PET® fluorescent dyes (Applied Biosystems, Inc., Foster City, CA, USA). .. All loci were amplified in the same multiplex reaction using the Qiagen multiplex PCR kit (Qiagen, Venlo, The Netherlands).

    Article Title: Characterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross-amplification in 10 other subgenera 1
    Article Snippet: .. Following the procedure developed by , the sequence-specific forward primers were marked at the 5′ end with an M13 tail (5′-TGTAAAACGACGGCCAGT-3′) (Eurofins), which was then labeled with 5-FAM, VIC, NED, or PET fluorescent dyes ( ) (Life Technologies). .. The PCR mix contained 1× PCR Green GoTaq (Promega Corporation), 0.2 mM of each dNTP, 0.16 mM of each reverse and fluorescent-labeled M13 primer, 0.04 mM of forward primer, 0.75 units GoTaq DNA Polymerase, and 50 ng of DNA template in a total volume of 15 μL.

    Article Title: Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification
    Article Snippet: .. After conjugating 6-FAM, HEX, NED or PET fluorescent dyes (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) to the 5′-end of each forward primer, we performed multiplex PCR for the seven selected primer sets using a Qiagen multiplex PCR kit (Qiagen, Limburg, The Netherlands). .. The 10-μL reaction mixture contained 1× Qiagen multiplex PCR master mix, 0.2 μM of each primer, 2 μL of distilled water, and 1 μL of DNA solution.

    Article Title: Development of 14 microsatellite markers in Odontites vernus s.l. (Orobanchaceae) and cross-amplification in related taxa 1
    Article Snippet: .. Eighteen loci were selected (see for discarding reasons) and tested on 140 O. vernus samples using a three-primer PCR protocol ( ) with the universal primer M13(–21) 5′-TGTAAAACGACGGCCAGT-3′ marked with 5-FAM, VIC, NED, or PET fluorescent dyes (Life Technologies; ). .. The PCR mix was as described above, except that 0.2 mM of each reverse and fluorescent-labeled M13 primer and 0.08 mM of the forward primer were used.

    Labeling:

    Article Title: Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera. Evolutionary divergence in life history traits among populations of the Lake Malawi cichlid fish Astatotilapia calliptera
    Article Snippet: .. Forward primers were labeled using 6‐FAM, NED, VIC, PET® fluorescent dyes (Applied Biosystems, Inc., Foster City, CA, USA). .. All loci were amplified in the same multiplex reaction using the Qiagen multiplex PCR kit (Qiagen, Venlo, The Netherlands).

    Article Title: Characterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross-amplification in 10 other subgenera 1
    Article Snippet: .. Following the procedure developed by , the sequence-specific forward primers were marked at the 5′ end with an M13 tail (5′-TGTAAAACGACGGCCAGT-3′) (Eurofins), which was then labeled with 5-FAM, VIC, NED, or PET fluorescent dyes ( ) (Life Technologies). .. The PCR mix contained 1× PCR Green GoTaq (Promega Corporation), 0.2 mM of each dNTP, 0.16 mM of each reverse and fluorescent-labeled M13 primer, 0.04 mM of forward primer, 0.75 units GoTaq DNA Polymerase, and 50 ng of DNA template in a total volume of 15 μL.

    Article Title: SSR markers for Filago subg. Filago (Gnaphalieae: Asteraceae) and cross‐amplification in three other subgeneraSSR markers for Filago subg. Filago (Gnaphalieae: Asteraceae) and cross‐amplification in three other subgenera
    Article Snippet: .. The sequence‐specific forward primers were marked using the universal primer M13(–21) 5′‐TGTAAAACGACGGCCAGT‐3′ (Schuelke, ) labeled with 5‐FAM, VIC, NED, or PET fluorescent dyes (Table ) (Life Technologies). .. The composition of the PCR mastermix for populations SA865 and SA1109 was as described above, except for the fluorescent‐labeled reverse primer (0.8 mM) and the forward primer (0.2 mM).

    Electrophoresis:

    Article Title: Sequencing and Characterization of Striped Venus Transcriptome Expand Resources for Clam Fishery Genetics
    Article Snippet: .. Forward primers were labelled with FAM, VIC, NED and PET fluorescent dyes (Applied Biosystems) to verify the electrophoresis-predicted polymorphism. .. A fraction of the PCR product was loaded on an Applied Biosystems 3130 XL automated sequencer (Liz500 as size standard, genotyping facility at www.bmr-genomics.com ) and allele sizes were assigned using GENEMARKER 1.71 (SoftGenetics, State College, Pennsylvania).

    Sequencing:

    Article Title: Characterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross-amplification in 10 other subgenera 1
    Article Snippet: .. Following the procedure developed by , the sequence-specific forward primers were marked at the 5′ end with an M13 tail (5′-TGTAAAACGACGGCCAGT-3′) (Eurofins), which was then labeled with 5-FAM, VIC, NED, or PET fluorescent dyes ( ) (Life Technologies). .. The PCR mix contained 1× PCR Green GoTaq (Promega Corporation), 0.2 mM of each dNTP, 0.16 mM of each reverse and fluorescent-labeled M13 primer, 0.04 mM of forward primer, 0.75 units GoTaq DNA Polymerase, and 50 ng of DNA template in a total volume of 15 μL.

    Article Title: SSR markers for Filago subg. Filago (Gnaphalieae: Asteraceae) and cross‐amplification in three other subgeneraSSR markers for Filago subg. Filago (Gnaphalieae: Asteraceae) and cross‐amplification in three other subgenera
    Article Snippet: .. The sequence‐specific forward primers were marked using the universal primer M13(–21) 5′‐TGTAAAACGACGGCCAGT‐3′ (Schuelke, ) labeled with 5‐FAM, VIC, NED, or PET fluorescent dyes (Table ) (Life Technologies). .. The composition of the PCR mastermix for populations SA865 and SA1109 was as described above, except for the fluorescent‐labeled reverse primer (0.8 mM) and the forward primer (0.2 mM).

    Polymerase Chain Reaction:

    Article Title: Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification
    Article Snippet: .. After conjugating 6-FAM, HEX, NED or PET fluorescent dyes (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) to the 5′-end of each forward primer, we performed multiplex PCR for the seven selected primer sets using a Qiagen multiplex PCR kit (Qiagen, Limburg, The Netherlands). .. The 10-μL reaction mixture contained 1× Qiagen multiplex PCR master mix, 0.2 μM of each primer, 2 μL of distilled water, and 1 μL of DNA solution.

    Article Title: Development of 14 microsatellite markers in Odontites vernus s.l. (Orobanchaceae) and cross-amplification in related taxa 1
    Article Snippet: .. Eighteen loci were selected (see for discarding reasons) and tested on 140 O. vernus samples using a three-primer PCR protocol ( ) with the universal primer M13(–21) 5′-TGTAAAACGACGGCCAGT-3′ marked with 5-FAM, VIC, NED, or PET fluorescent dyes (Life Technologies; ). .. The PCR mix was as described above, except that 0.2 mM of each reverse and fluorescent-labeled M13 primer and 0.08 mM of the forward primer were used.

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  • 89
    Thermo Fisher ned fluorescent dyes
    Ned Fluorescent Dyes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ned fluorescent dyes/product/Thermo Fisher
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    Thermo Fisher fam fluorescent dyes
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