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Millipore pet 44a
Pet 44a, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet 44a/product/Millipore
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
pet 44a - by Bioz Stars, 2020-01
92/100 stars

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Clone Assay:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: .. The amplified β2-GP I products were digested with HindIII and BamHI restriction enzymes (Takara Bio, Inc.) and cloned into multiple clone sites of pET-44a (+) (Novagen; Merck KGaA). .. In order to construct the prokaryotic expression vector, the structural gene of human β2-GP I protein was amplified by PCR and inserted into the pET-44a (+) plasmid.

Transfection:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: Following the aforementioned procedure, 1 ng of constructed vector transfected into E. coli BL21 (DE3, DE3:T7 RNA polymerase gene) cells (Vazyme, Piscataway, NJ, USA) and one single clone from the solid agar Petri dish (containing 100 ug/ml ampicillin), was amplified in liquid agar medium (containing 100 ug/ml ampicillin). .. The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst.

Positron Emission Tomography:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: .. The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst. .. The induced protein in the lysate was further purified by Ni-NTA agarose (Qiagen, Germantown, MD, USA) and identified by western blotting as described below.

Article Title: Structure-based analysis of the guanine nucleotide exchange factor SmgGDS reveals armadillo-repeat motifs and key regions for activity and GTPase binding
Article Snippet: .. The following cDNA were subcloned into the pET-44a(+) vector (Novagen): h RhoA (UniProt ID: P61586-1, aa 1–193) harbored His6 and PreScission protease recognition sequences at the N terminus. h RhoAL193A was generated by PCR-based site-directed mutagenesis. .. The host cell and protein expression procedure were the same as described above.

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: .. The amplified β2-GP I products were digested with HindIII and BamHI restriction enzymes (Takara Bio, Inc.) and cloned into multiple clone sites of pET-44a (+) (Novagen; Merck KGaA). .. In order to construct the prokaryotic expression vector, the structural gene of human β2-GP I protein was amplified by PCR and inserted into the pET-44a (+) plasmid.

Sonication:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: .. The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst. .. The induced protein in the lysate was further purified by Ni-NTA agarose (Qiagen, Germantown, MD, USA) and identified by western blotting as described below.

Article Title: Structure-based analysis of the guanine nucleotide exchange factor SmgGDS reveals armadillo-repeat motifs and key regions for activity and GTPase binding
Article Snippet: The harvested cells were sonicated in buffer A (20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 1 m m DTT) with 1 m m PMSF. .. The following cDNA were subcloned into the pET-44a(+) vector (Novagen): h RhoA (UniProt ID: P61586-1, aa 1–193) harbored His6 and PreScission protease recognition sequences at the N terminus. h RhoAL193A was generated by PCR-based site-directed mutagenesis.

Mutagenesis:

Article Title: Structure-based analysis of the guanine nucleotide exchange factor SmgGDS reveals armadillo-repeat motifs and key regions for activity and GTPase binding
Article Snippet: .. The following cDNA were subcloned into the pET-44a(+) vector (Novagen): h RhoA (UniProt ID: P61586-1, aa 1–193) harbored His6 and PreScission protease recognition sequences at the N terminus. h RhoAL193A was generated by PCR-based site-directed mutagenesis. .. The host cell and protein expression procedure were the same as described above.

Size-exclusion Chromatography:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: .. The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst. .. The induced protein in the lysate was further purified by Ni-NTA agarose (Qiagen, Germantown, MD, USA) and identified by western blotting as described below.

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: The following thermocycling conditions were used for the PCR: Initial denaturation for 1 min at 95°C; 30 cycles of 95°C for 10 sec, 55°C for 10 sec and 72°C for 60 sec, using 2X PCR Solution Premix Taq™ (Takara Bio, Inc., Otsu, Japan). .. The amplified β2-GP I products were digested with HindIII and BamHI restriction enzymes (Takara Bio, Inc.) and cloned into multiple clone sites of pET-44a (+) (Novagen; Merck KGaA).

Construct:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: Following the aforementioned procedure, 1 ng of constructed vector transfected into E. coli BL21 (DE3, DE3:T7 RNA polymerase gene) cells (Vazyme, Piscataway, NJ, USA) and one single clone from the solid agar Petri dish (containing 100 ug/ml ampicillin), was amplified in liquid agar medium (containing 100 ug/ml ampicillin). .. The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst.

Purification:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: Paragraph title: Purification of the His-tagged protein ... The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst.

Article Title: Structure-based analysis of the guanine nucleotide exchange factor SmgGDS reveals armadillo-repeat motifs and key regions for activity and GTPase binding
Article Snippet: The supernatant solution containing the tag-removed SmgGDS was collected, and further purification was performed with HiTrap Q (GE Healthcare) with buffer C (20 m m Tris-HCl (pH 7.5) and 1 m m DTT) and buffer D (20 m m Tris-HCl (pH 7.5), 1 m m DTT, and 1 m NaCl) and with Superdex 200 prep grade (GE Healthcare) with buffer A. .. The following cDNA were subcloned into the pET-44a(+) vector (Novagen): h RhoA (UniProt ID: P61586-1, aa 1–193) harbored His6 and PreScission protease recognition sequences at the N terminus. h RhoAL193A was generated by PCR-based site-directed mutagenesis.

Acid Assay:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst. .. Protein concentration was determined using Bicinchoninic Acid assay kit (Pierce; Thermo Fisher Scientific, Inc.).

Sequencing:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: .. The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst. .. The induced protein in the lysate was further purified by Ni-NTA agarose (Qiagen, Germantown, MD, USA) and identified by western blotting as described below.

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: Based on the sequence information from the National Center for Biotechnology Information database , the β2-GP I gene (GenBank: ) was amplified using PCR with primers designed to generate Hind III and BamH I restriction sites at the 5′ and 3′ends of the amplified fragments, respectively. .. The amplified β2-GP I products were digested with HindIII and BamHI restriction enzymes (Takara Bio, Inc.) and cloned into multiple clone sites of pET-44a (+) (Novagen; Merck KGaA).

Incubation:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: .. The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst. .. The induced protein in the lysate was further purified by Ni-NTA agarose (Qiagen, Germantown, MD, USA) and identified by western blotting as described below.

Amplification:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: Following the aforementioned procedure, 1 ng of constructed vector transfected into E. coli BL21 (DE3, DE3:T7 RNA polymerase gene) cells (Vazyme, Piscataway, NJ, USA) and one single clone from the solid agar Petri dish (containing 100 ug/ml ampicillin), was amplified in liquid agar medium (containing 100 ug/ml ampicillin). .. The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst.

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: .. The amplified β2-GP I products were digested with HindIII and BamHI restriction enzymes (Takara Bio, Inc.) and cloned into multiple clone sites of pET-44a (+) (Novagen; Merck KGaA). .. In order to construct the prokaryotic expression vector, the structural gene of human β2-GP I protein was amplified by PCR and inserted into the pET-44a (+) plasmid.

Expressing:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: .. The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst. .. The induced protein in the lysate was further purified by Ni-NTA agarose (Qiagen, Germantown, MD, USA) and identified by western blotting as described below.

Article Title: Structure-based analysis of the guanine nucleotide exchange factor SmgGDS reveals armadillo-repeat motifs and key regions for activity and GTPase binding
Article Snippet: The following cDNA were subcloned into the pET-44a(+) vector (Novagen): h RhoA (UniProt ID: P61586-1, aa 1–193) harbored His6 and PreScission protease recognition sequences at the N terminus. h RhoAL193A was generated by PCR-based site-directed mutagenesis. .. The host cell and protein expression procedure were the same as described above.

Protein Concentration:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst. .. Protein concentration was determined using Bicinchoninic Acid assay kit (Pierce; Thermo Fisher Scientific, Inc.).

Polymerase Chain Reaction:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: In order to construct the prokaryotic expression vector, the structural gene of human β2-GP I protein was amplified by PCR and inserted into the pET-44a (+) plasmid. .. The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst.

Article Title: Structure-based analysis of the guanine nucleotide exchange factor SmgGDS reveals armadillo-repeat motifs and key regions for activity and GTPase binding
Article Snippet: .. The following cDNA were subcloned into the pET-44a(+) vector (Novagen): h RhoA (UniProt ID: P61586-1, aa 1–193) harbored His6 and PreScission protease recognition sequences at the N terminus. h RhoAL193A was generated by PCR-based site-directed mutagenesis. .. The host cell and protein expression procedure were the same as described above.

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: The following thermocycling conditions were used for the PCR: Initial denaturation for 1 min at 95°C; 30 cycles of 95°C for 10 sec, 55°C for 10 sec and 72°C for 60 sec, using 2X PCR Solution Premix Taq™ (Takara Bio, Inc., Otsu, Japan). .. The amplified β2-GP I products were digested with HindIII and BamHI restriction enzymes (Takara Bio, Inc.) and cloned into multiple clone sites of pET-44a (+) (Novagen; Merck KGaA).

Western Blot:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst. .. The induced protein in the lysate was further purified by Ni-NTA agarose (Qiagen, Germantown, MD, USA) and identified by western blotting as described below.

Lysis:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: .. The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst. .. The induced protein in the lysate was further purified by Ni-NTA agarose (Qiagen, Germantown, MD, USA) and identified by western blotting as described below.

Article Title: Structure-based analysis of the guanine nucleotide exchange factor SmgGDS reveals armadillo-repeat motifs and key regions for activity and GTPase binding
Article Snippet: Following cell lysis and purification, the steps were almost the same between the wild type and the SeMet-labeled protein. .. The following cDNA were subcloned into the pET-44a(+) vector (Novagen): h RhoA (UniProt ID: P61586-1, aa 1–193) harbored His6 and PreScission protease recognition sequences at the N terminus. h RhoAL193A was generated by PCR-based site-directed mutagenesis.

Recombinant:

Article Title: Structure-based analysis of the guanine nucleotide exchange factor SmgGDS reveals armadillo-repeat motifs and key regions for activity and GTPase binding
Article Snippet: Paragraph title: Preparation of recombinant SmgGDS, RhoA, and FTase ... The following cDNA were subcloned into the pET-44a(+) vector (Novagen): h RhoA (UniProt ID: P61586-1, aa 1–193) harbored His6 and PreScission protease recognition sequences at the N terminus. h RhoAL193A was generated by PCR-based site-directed mutagenesis.

RNA Extraction:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: The cDNA from Huh-7 cells was used as template and the following primers were used: 5′-CGCGGATCCATGATTTCTCCAGT-3′ and 5′-CCCAAGCTTTTAGCATGGCTTTAC-3′; RNA extraction was conducted as described below. .. The amplified β2-GP I products were digested with HindIII and BamHI restriction enzymes (Takara Bio, Inc.) and cloned into multiple clone sites of pET-44a (+) (Novagen; Merck KGaA).

Plasmid Preparation:

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: .. The vector of pET-44a(+) encoding the His-tag sequence and the expression of fusion protein rhβ2-GP I was induced by 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich; Merck KGaA); the bacteria were incubated at 25°C, 100 rpm/min for 2, 4 or 6 h. Subsequently, the bacteria was lysed with lysis buffer (50 mM NaH2 PO4 , 300 mM NaCl, 5 mM imidazole and 1 mg/ml lysozyme, pH=8.0) then sonicated 6 times at 200 W in 10 sec bursts with a 10 sec cooling period between each burst. .. The induced protein in the lysate was further purified by Ni-NTA agarose (Qiagen, Germantown, MD, USA) and identified by western blotting as described below.

Article Title: Structure-based analysis of the guanine nucleotide exchange factor SmgGDS reveals armadillo-repeat motifs and key regions for activity and GTPase binding
Article Snippet: .. The following cDNA were subcloned into the pET-44a(+) vector (Novagen): h RhoA (UniProt ID: P61586-1, aa 1–193) harbored His6 and PreScission protease recognition sequences at the N terminus. h RhoAL193A was generated by PCR-based site-directed mutagenesis. .. The host cell and protein expression procedure were the same as described above.

Article Title: Effect and mechanism of the aβ2-GP I/rhβ2-GP I complex on JEG-3 cell proliferation, migration and invasion
Article Snippet: Paragraph title: Plasmid encoding β2-GP I ... The amplified β2-GP I products were digested with HindIII and BamHI restriction enzymes (Takara Bio, Inc.) and cloned into multiple clone sites of pET-44a (+) (Novagen; Merck KGaA).

Generated:

Article Title: Structure-based analysis of the guanine nucleotide exchange factor SmgGDS reveals armadillo-repeat motifs and key regions for activity and GTPase binding
Article Snippet: .. The following cDNA were subcloned into the pET-44a(+) vector (Novagen): h RhoA (UniProt ID: P61586-1, aa 1–193) harbored His6 and PreScission protease recognition sequences at the N terminus. h RhoAL193A was generated by PCR-based site-directed mutagenesis. .. The host cell and protein expression procedure were the same as described above.

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    Millipore 42a 44a
    42a 44a, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/42a 44a/product/Millipore
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    42a 44a - by Bioz Stars, 2020-01
    79/100 stars
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