Structured Review

GeneTex peroxidase conjugated secondary antibody
Peroxidase Conjugated Secondary Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peroxidase conjugated secondary antibody/product/GeneTex
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
peroxidase conjugated secondary antibody - by Bioz Stars, 2020-07
92/100 stars

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Electrophoresis:

Article Title: Antizyme inhibitor 1 genetic polymorphisms associated with diabetic patients validated in the livers of diabetic mice
Article Snippet: .. The tissue lysate was obtained through denaturing electrophoresis by SDS-PAGE on a 12.5% with 60 mg protein loaded per lane, electrotransferred to a polyvinylidene difluoride membrane, the blots was incubated with blocking buffer (1X PBS and 5% nonfat dry milk) for 1 h at room temperature and then probed with primary anti-AZIN1 antibodies (1:1,000; cat. no. orb154904; Biorbyt, Ltd.) overnight at 4°C, followed by incubation with horseradish peroxidase-conjugated secondary antibody (1:5,000; cat. no. GTX213110; GeneTex) for 1 hour at RT. .. To control equal loading of total protein in all lanes, blots were stained with mouse anti-β-actin antibody (1:5,000; cat. no. ab8226; Abcam).

Incubation:

Article Title: Histone Deacetylase Inhibitor, Trichostatin A, Synergistically Enhances Paclitaxel-Induced Cytotoxicity in Urothelial Carcinoma Cells by Suppressing the ERK Pathway
Article Snippet: .. After two washes with Tris-buffered saline containing 0.05% Tween 20 (TBST), the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Genetex) at recommended dilution ratios in PBS at room temperature for 2 h. The antibody-marked membranes were again washed twice with TBST and visualized using enhanced chemiluminescence substrates (Merck Millipore) in an ImageQuant LAS 4000 (GE Healthcare) system. .. Apoptosis Assay The apoptosis assay was performed according to the manufacturer’s protocol using Muse Annexin V and a Dead Cell Kit (Merck Millipore).

Article Title: Suppression of Angiogenesis by Targeting Cyclin-Dependent Kinase 7 in Human Umbilical Vein Endothelial Cells and Renal Cell Carcinoma: An In Vitro and In Vivo Study
Article Snippet: .. The membranes were then washed three times with TBST for 10 min each and were incubated at room temperature for 1 h with horseradish peroxidase-conjugated secondary antibodies (GeneTex). .. After washing twice with TBST, antibody-bound membranes were visualized with enhanced chemiluminescence Western blot detection reagents (Millipore and Biotools).

Article Title: Antizyme inhibitor 1 genetic polymorphisms associated with diabetic patients validated in the livers of diabetic mice
Article Snippet: .. The tissue lysate was obtained through denaturing electrophoresis by SDS-PAGE on a 12.5% with 60 mg protein loaded per lane, electrotransferred to a polyvinylidene difluoride membrane, the blots was incubated with blocking buffer (1X PBS and 5% nonfat dry milk) for 1 h at room temperature and then probed with primary anti-AZIN1 antibodies (1:1,000; cat. no. orb154904; Biorbyt, Ltd.) overnight at 4°C, followed by incubation with horseradish peroxidase-conjugated secondary antibody (1:5,000; cat. no. GTX213110; GeneTex) for 1 hour at RT. .. To control equal loading of total protein in all lanes, blots were stained with mouse anti-β-actin antibody (1:5,000; cat. no. ab8226; Abcam).

Article Title: Caspase 3 inactivation protects against hepatic cell death and ameliorates fibrogenesis in a diet induced NASH model
Article Snippet: .. Membranes were incubated with peroxidase-conjugated secondary antibody (dilution 1:10,000) (GeneTex, Irvine, CA, USA), protein bands were visualized with the enhanced chemiluminescence reagent and digitized using a CCD camera (ChemiDoc®, Biorad, Hercules, CA, USA). .. Expression intensity was quantified by ImageLab (Biorad).

Article Title: Increased Expression of Ecto-NOX Disulfide-thiol Exchanger 1 (ENOX1) in Diabetic Mice Retina and its Involvement in Diabetic Retinopathy Development
Article Snippet: .. The membranes were incubated with anti-ENOX1 primary antibody (dilution 1:500; Novus Biological, Littleton, CO, USA) overnight at 4˚C, followed by incubation with horseradish peroxidase-conjugated secondary antibody (GeneTex, Austin, TX, USA) at room temperature for 1 h. Anti-β-actin (dilution 1:6,000; Novus Biological) was used as an internal control. .. Protein signal was detected using an enhanced chemiluminescence system (Syngene’s ChemiGenius XE Bio Imaging System, Maryland, USA).

SDS Page:

Article Title: Antizyme inhibitor 1 genetic polymorphisms associated with diabetic patients validated in the livers of diabetic mice
Article Snippet: .. The tissue lysate was obtained through denaturing electrophoresis by SDS-PAGE on a 12.5% with 60 mg protein loaded per lane, electrotransferred to a polyvinylidene difluoride membrane, the blots was incubated with blocking buffer (1X PBS and 5% nonfat dry milk) for 1 h at room temperature and then probed with primary anti-AZIN1 antibodies (1:1,000; cat. no. orb154904; Biorbyt, Ltd.) overnight at 4°C, followed by incubation with horseradish peroxidase-conjugated secondary antibody (1:5,000; cat. no. GTX213110; GeneTex) for 1 hour at RT. .. To control equal loading of total protein in all lanes, blots were stained with mouse anti-β-actin antibody (1:5,000; cat. no. ab8226; Abcam).

Blocking Assay:

Article Title: Antizyme inhibitor 1 genetic polymorphisms associated with diabetic patients validated in the livers of diabetic mice
Article Snippet: .. The tissue lysate was obtained through denaturing electrophoresis by SDS-PAGE on a 12.5% with 60 mg protein loaded per lane, electrotransferred to a polyvinylidene difluoride membrane, the blots was incubated with blocking buffer (1X PBS and 5% nonfat dry milk) for 1 h at room temperature and then probed with primary anti-AZIN1 antibodies (1:1,000; cat. no. orb154904; Biorbyt, Ltd.) overnight at 4°C, followed by incubation with horseradish peroxidase-conjugated secondary antibody (1:5,000; cat. no. GTX213110; GeneTex) for 1 hour at RT. .. To control equal loading of total protein in all lanes, blots were stained with mouse anti-β-actin antibody (1:5,000; cat. no. ab8226; Abcam).

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