peroxidase conjugated secondary antibodies  (Nacalai)

 
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    Structured Review

    Nacalai peroxidase conjugated secondary antibodies
    Peroxidase Conjugated Secondary Antibodies, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated secondary antibodies/product/Nacalai
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated secondary antibodies - by Bioz Stars, 2020-07
    92/100 stars

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    Western Blot:

    Article Title: Poly(ADP-ribose)polymerase inhibition counteracts renal hypertrophy and multiple manifestations of peripheral neuropathy in diabetic Akita mice
    Article Snippet: .. The horseradish peroxidase-conjugated secondary antibodies were then applied for 1 h. In the Western blot analysis of phosphorylated p38 MAPK, the signal enhancer HIKARI (Nacalai USA, San Diego, CA) was applied with both primary and secondary antibodies according to the manufacturer’s instructions. .. After extensive washing, protein bands detected by the antibodies were visualized with the Amersham ECL™ Western Blotting Detection Reagent (Little Chalfont, Buckinghamshire, UK).

    Incubation:

    Article Title: Endocrine therapy-resistant breast cancer model cells are inhibited by soybean glyceollin I through Eleanor non-coding RNA
    Article Snippet: .. The membrane was washed with PBS containing 0.3% Tween 20 three times for 5 min each, and incubated with horseradish peroxidase-conjugated secondary antibodies for 60 min. After the membrane was washed with PBS containing 0.3% Tween 20 three times for 5 min each, the signals were visualized with Chemi-Lumi One Ultra solution (nacalai tesque), and detected by Amersham Imager 600 (GE Healthcare). .. Relative cell proliferation was measured with colorimetric assays using the Cell Counting Kit-8 (Dojindo), according to the manufacture’s protocol.

    Article Title: ATP/P2X7 receptor signaling as a potential anti-inflammatory target of natural polyphenols
    Article Snippet: .. After washing, the membrane was incubated with horseradish peroxidase-conjugated secondary antibody (1:2000) in TTBS containing 5% skim milk at room temperature for 1 h. After washing, chemiluminescent detection was performed using Chemi-Lumi One L or Chemi-Lumi One Super (Nacalai Tesque, Kyoto, Japan) and visualized by Ez-Capture MG (Atto Corp., Tokyo, Japan). .. Real-time reverse transcription-polymerase chain reaction The total RNA was extracted from the cells using Sepasol-RNA I Super G (Nacalai Tesque, Kyoto, Japan). cDNA was synthesized using ReverTra Ace qPCR RT Master Mix with gDNA Remover (Code No. FSQ-301, Toyobo Co., Ltd., Osaka, Japan).

    Article Title: Inhibition of melanin production by anthracenone dimer glycosides isolated from Cassia auriculata seeds
    Article Snippet: .. After washing with T-TBS, blocking was carried out with Blocking One (Nacalai Tesque, Inc., Kyoto, Japan) for 1 h, and incubation was commenced with primary antibody against each protein in 5% Blocking One/T-TBS solution (0.2 μg/mL) for 1 h. After washing with T-TBS, the membrane was incubated with horseradish peroxidase-conjugated secondary antibody in 5% Blocking One/T-TBS solution (0.3 μg/mL) for 1 h. Immunoreactive proteins were detected using a chemiluminescence kit (Chemi-Lumi One; Nacalai Tesque) according to the manufacturer’s instructions. .. Each target band was measured by a lumino image analyzer (LAS-4000 mini; Fujifilm, Tokyo Japan); Multi Gauge V3.0 software was used to analyze the data (Fujifilm).

    Blocking Assay:

    Article Title: Data supporting Arf6 regulation of Schwann cell differentiation and myelination
    Article Snippet: .. The transferred membranes were blocked with a Blocking One kit (Nacalai) and immunoblotted using primary antibodies (anti-Arf1 and anti-Arf6 [Santa Cruz Biotechnology], and MPZ and actin [MBL]), followed by peroxidase-conjugated secondary antibodies (Nacalai). .. The bound antibodies were detected using an ImmunoStar Zeta kit (Wako).

    Article Title: Inhibition of melanin production by anthracenone dimer glycosides isolated from Cassia auriculata seeds
    Article Snippet: .. After washing with T-TBS, blocking was carried out with Blocking One (Nacalai Tesque, Inc., Kyoto, Japan) for 1 h, and incubation was commenced with primary antibody against each protein in 5% Blocking One/T-TBS solution (0.2 μg/mL) for 1 h. After washing with T-TBS, the membrane was incubated with horseradish peroxidase-conjugated secondary antibody in 5% Blocking One/T-TBS solution (0.3 μg/mL) for 1 h. Immunoreactive proteins were detected using a chemiluminescence kit (Chemi-Lumi One; Nacalai Tesque) according to the manufacturer’s instructions. .. Each target band was measured by a lumino image analyzer (LAS-4000 mini; Fujifilm, Tokyo Japan); Multi Gauge V3.0 software was used to analyze the data (Fujifilm).

    Article Title: Data on the effect of hypomyelinating leukodystrophy 6 (HLD6)-associated mutations on the TUBB4A properties
    Article Snippet: .. The electrophoretically separated proteins were transferred to PVDF membranes, blocked with the Blocking One reagent, and immunoblotted using primary antibodies (a monoclonal tubulin alpha antibody [MBL] or a monoclonal anti-GFP antibody [MBL]), followed by peroxidase-conjugated secondary antibodies (Nacalai Tesque). .. The bound antibodies were detected using the ImmunoStar Zeta kit (Wako).

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    Nacalai hrp conjugated secondary antibody
    Hrp Conjugated Secondary Antibody, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated secondary antibody/product/Nacalai
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated secondary antibody - by Bioz Stars, 2020-07
    92/100 stars
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    Nacalai horseradish peroxidase conjugated secondary antibodies
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary antibodies/product/Nacalai
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary antibodies - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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