peroxidase conjugated anti rabbit (Thermo Fisher)
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Name:
Rabbit anti Rat IgG IgM IgA Peroxidase
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Catalog Number:
sa1-36149
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Structured Review
Thermo Fisher
peroxidase conjugated anti rabbit
https://www.bioz.com/result/peroxidase conjugated anti rabbit/product/Thermo Fisher
Average 99 stars, based on 8 article reviews
Price from $9.99 to $1999.99
https://www.bioz.com/result/peroxidase conjugated anti rabbit/product/Thermo Fisher
Average 99 stars, based on 8 article reviews
Price from $9.99 to $1999.99
peroxidase conjugated anti rabbit - by Bioz Stars,
2021-01
99/100 stars
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Purification:Article Title: Signaling through regulated transcription factor interaction: mapping of a regulatory interaction domain in the Myb-related Bas1p Article Snippet: .. Western blot analysis was performed with the ECL Plus™ western blot kit (Amersham) with purified anti-Bas1p (1 µg purified IgG/ml) as primary antibody and Incubation:Article Title: Identification of di-substituted ureas that prevent growth of trypanosomes through inhibition of translation initiation Article Snippet: .. The primary antibody was washed 3 times in TBS-T for 10 min and incubated for 1 hour with Article Title: Knockdown of GSK3β increases basal autophagy and AMPK signalling in nutrient-laden human aortic endothelial cells Article Snippet: .. Following washing in TBST, membranes were incubated in Article Title: Profiling and bioinformatic analysis of circular RNA expression regulated by c-Myc Article Snippet: .. After washed with TBST, membrane was incubated in horseradish Article Title: Mucosal immunoglobulins protect the olfactory organ of teleost fish against parasitic infection Article Snippet: .. Thereafter, the membranes were blocked with 8% skim milk and incubated with anti-trout IgT (rabbit pAb), anti-trout IgM (mouse monoclonal antibody (mAb)) or biotinylated anti-trout IgD (mouse mAb) antibodies followed by incubation with Lysis:Article Title: The LIM-Only Protein PINCH Directly Interacts with Integrin-Linked Kinase and Is Recruited to Integrin-Rich Sites in Spreading Cells Article Snippet: .. After five washes with lysis buffer, human ILK associated with MBP-PINCH was detected by immunoblotting with an affinity-purified rabbit polyclonal anti-human ILK antibody (91-5; 69 ng/ml), a horseradish Western Blot:Article Title: Signaling through regulated transcription factor interaction: mapping of a regulatory interaction domain in the Myb-related Bas1p Article Snippet: .. Western blot analysis was performed with the ECL Plus™ western blot kit (Amersham) with purified anti-Bas1p (1 µg purified IgG/ml) as primary antibody and Affinity Purification:Article Title: The LIM-Only Protein PINCH Directly Interacts with Integrin-Linked Kinase and Is Recruited to Integrin-Rich Sites in Spreading Cells Article Snippet: .. After five washes with lysis buffer, human ILK associated with MBP-PINCH was detected by immunoblotting with an affinity-purified rabbit polyclonal anti-human ILK antibody (91-5; 69 ng/ml), a horseradish SDS Page:Article Title: Manipulation of Cell Cycle and Chromatin Configuration by Means of Cell-Penetrating Geminin Article Snippet: .. Proteins were separated by means of SDS-PAGE, transferred to Immobilon-P (Merck-Millipore, Billerica, MA), immunoblotted with primary antibodies, and visualized with a horseradish |
![SB203580, an RIP2 inhibitor downstream of nucleotide-binding oligomerization domain (NOD)2, attenuates CLP-induced sepsis. (A) Peritoneal cells of WT mice were cultured with SB203580 and/or MDP for 24 h, and IL-1β and IL-10 concentrations were measured in culture fractions. (B) Molecules related to NOD2-mediated signal transduction were blotted using peritoneal cells obtained from WT and Nod2 −/− mice injected with SB203580 or PBS 24 h after CLP. (C) Serum and peritoneal IL-1β, IL-10, and C5a levels were estimated in WT (n = 4) and Nod2 −/− (n = 3) mice injected with SB203580 (n = 4 in WT, n = 3 in Nod2 −/− ) or PBS 24 h after CLP by ELISA. (D) The levels of CD55 expression on F4/80 − Ly6-G + cells from WT (n = 3) and WT mice injected with SB203580 (n = 3) were measured 24 h after CLP. (mean fluorescence intensity [MFI] of CD55 expression in the panels, diagrams filled with gray for istotype-matched control IgG, solid lines for CD55) (E) The percentages of surviving mice were estimated during CLP-induced sepsis ( a P = 0.0312, log-rank test, n = 6–8 per group; WT mice injected with SB203580 vs. PBS). *P](https://storage.googleapis.com/bioz_article_images/PMC3649968/ppat.1003351.g006.jpg)
![IL-1β-dependent IL-10 production mediated by nucleotide-binding oligomerization domain (NOD) 2 enhances C5a generation by suppressing CD55 expression on Ly6-G + cells during sepsis. (A) CD55 and CR1/2 expression on gated F4/80 − Ly6-G + peritoneal cells from WT, Nod2 −/− , and Nod2 −/− mice injected with recombinant IL-1β or IL-10 was estimated 24 h after CLP (mean fluorescence intensity [MFI] of CD55 expression in the panels). (B) CD55 expression on gated F4/80 − Ly6-G + peritoneal cells from Il-10 −/− or Il-10 −/− mice injected with recombinant IL-1β was estimated 24 h after CLP (MFI of CD55 expression in the panels) (C) To block IL-10 receptor engagement in vivo , anti-IL10 receptor mAbs were i.p. injected into WT and Nod2 −/− mice administered recombinant IL-10 during CLP-induced sepsis. CD55 expression on gated F4/80 − Ly6-G + peritoneal cells from these mice 24 h after CLP was evaluated. (D) The levels of CD55 expression on F4/80 − Ly6-G + peritoneal cells were compared in WT, Nod2 −/− , IL-10 −/− , and Il-1r −/− mice 24 h after CLP. (A–D) anti-CD55 mAb (lines) and control IgG (diagrams filled with gray) were used. (E) Peritoneal cells from WT and Nod2 −/− mice 24 h after CLP were blotted for Bb factor. (F) Peritoneal cells from WT and Nod2 −/− mice 12 h after CLP were incubated with RPMI media containing 10% WT mouse serum for 24 h. (G and H) To evaluate the effect of CD55 on C5a generation in vivo , WT, Nod2 −/− , (G) or Nod2 −/− mice given recombinant IL-10 (H) were i.p. injected with soluble CD55 12 h after CLP. Serum and peritoneal C5a levels and the survival percentages of these mice were measured during CLP-induced sepsis ( a P = 0.0124, log-rank test; WT [n = 8], Nod2 −/− [n = 8 ], and soluble CD55-injected WT [n = 6 ] or Nod2 −/− mice [n = 8 ] in G, a P = 0.0024, log-rank test; Nod2 −/− mice [n = 8], Nod2 −/− mice injected with recombinant IL-10 [n = 8] or recombinant IL-10 and soluble CD55 [n = 6] in H). *P](https://storage.googleapis.com/bioz_article_images/PMC3649968/ppat.1003351.g005.jpg)