Structured Review

Jackson Immuno peroxidase affinipure donkey anti goat igg
Peroxidase Affinipure Donkey Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peroxidase affinipure donkey anti goat igg/product/Jackson Immuno
Average 99 stars, based on 29 article reviews
Price from $9.99 to $1999.99
peroxidase affinipure donkey anti goat igg - by Bioz Stars, 2020-04
99/100 stars

Images

Related Articles

Cytometry:

Article Title: Platelet Surface-Associated Activation and Secretion-Mediated Inhibition of Coagulation Factor XII
Article Snippet: .. Materials The following materials were used: thrombin (Haematologic Technologies; Essex Junction, VT, USA); fXIIa and fXII (Enzyme Research Laboratories; South Bend, IN, USA); AlignFlow flow cytometry alignment beads (2.5 μm for 488-nm excitation), fluorescein-5-isothiocyanate (FITC), and tetramethylrhodamine (TMRM) (Molecular Probes; Eugene, OR, USA); unlabelled and FITC-annexin V (BD Biosciences; San Jose, CA, USA); AlexaFluor-647-annexin V (Biolegend; San Diego, CA, USA); prostaglandin E1 (PGE1) (MP Biochemicals; Irvine, CA, USA); PPACK (EMD Chemicals; Gibbstown, NJ, USA); the chromomeric substrates S2238 and S2302 (Chromogenix; Milano, Italy); HEPES, bovine serum albumin, Sepharose CL-2B, Protein G sepharose, apyrase grade VII, mepacrine (quinacrine), PBS, EDTA and DMSO (Sigma-Aldrich; St Louis, MO, USA); calpeptin and MDL 28170 (Tocris Bioscience; Ellisville, MO, USA); G1/C1-inhibitor and polyclonal goat anti-human serpin G1/C1-inhibitor antibody (R & D Systems; Minneapolis, MN, USA); goat polyclonal anti-human factor XII antibody (LifeSpan BioSciences, Inc., Seattle, WA, USA); peroxidase-AffiniPure donkey anti-goat IgG (Jackson ImmunoResearch Laboratories; West Grove, PA, USA); egg phosphatidylcholine and egg phosphatidylserine (Avanti Polar Lipids; Alabaster, AL, USA); DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) (AppliChem; Darmstadt, Germany); and a kit to estimate fXII activation (Renam; Moscow, Russia). ..

Blocking Assay:

Article Title: Comparative Analysis of African and Asian Lineage-Derived Zika Virus Strains Reveals Differences in Activation of and Sensitivity to Antiviral Innate Immunity
Article Snippet: After a blocking step with a 4% bovine serum albumin (BSA) solution, the membranes were incubated with the following primary antibodies at 4°C overnight: rabbit anti-IRF3 S386 (1:500) (ab76493; Abcam, MA, USA), rabbit anti-IRF3 S396 (1:1,000) (no. 29047; Cell Signaling Technology [CST], Danvers, MA, USA), rabbit anti-IRF3 (1:1,000) (no. 4302; CST), rabbit anti-IFIT1 no. 971 (1:1,000) and rabbit anti-MxA no. 340B (1:500) (both made at UT Southwestern Antibody Core facility by repeated injections of synthetic peptides into rabbits), mouse anti-pan-actin C4 (1:3,000) (MAB1501; Millipore), rabbit anti-ZIKV-NS5 (1:2,500) (GTX133312; GeneTex, Irvine, CA, USA), goat anti-WNV-NS3 (1:1,000) (BAF2901; R & D Systems, Minneapolis, MN, USA), goat anti-parainfluenza virus 1 (B65121G-1; Amsbio, Cambridge, MA, USA), mouse anti-RIG-I AlmeI (1:1,000) (AG-20B-0009-C100; AdipoGen, San Diego, CA, USA), rabbit anti-MDA5 (1:500) (29020; IBL, Minneapolis, MN, USA), rabbit anti-LGP2 (1:500) (no. 29030; IBL), and rabbit anti-Cardif AT107 (1:1,000) (ALX-210-929-C100; Enzo Life Sciences, Farmingdale, NY, USA). .. Bound primary antibodies were detected with 1:1,000 diluted horseradish peroxidase-coupled secondary antibodies: donkey anti-mouse antibody (715-035-150), donkey anti-rabbit antibody (711-035-152), donkey anti-goat antibody (705-035-003) (all from Jackson Immunoresearch, West Grove, PA, USA), and an ECL prime reagent (GE Healthcare, Pittsburgh, PA, USA) on a ChemiDoc Imager (Bio-Rad).

Article Title: Proteomic identification of FHL1 as the protein mutated in human reducing body myopathy
Article Snippet: .. After washing, the membrane was incubated 1 hour at room temperature with HRP-conjugated secondary antibody (donkey anti-goat HRP; Jackson ImmunoResearch Laboratories; 705-035-147, 1:15,000) diluted in blocking buffer. .. Detection was performed using the ECL system by Amersham.

Electrophoresis:

Article Title: Proteomic identification of FHL1 as the protein mutated in human reducing body myopathy
Article Snippet: After lysis (10% SDS, 10 mM EDTA, 10 mM EGTA, 50 mM DTT, 6% glycerol, 60 mM Tris-HCl) and sonication, 0.5 mg of muscle tissue in loading buffer (Laemmli Sample Buffer; Bio-Rad; 5% β-mercaptoethanol) were submitted to electrophoresis on a 10% SDS-PAGE gel and then transferred onto a PVDF membrane (Millipore). .. After washing, the membrane was incubated 1 hour at room temperature with HRP-conjugated secondary antibody (donkey anti-goat HRP; Jackson ImmunoResearch Laboratories; 705-035-147, 1:15,000) diluted in blocking buffer.

Incubation:

Article Title: Comparative Analysis of African and Asian Lineage-Derived Zika Virus Strains Reveals Differences in Activation of and Sensitivity to Antiviral Innate Immunity
Article Snippet: After a blocking step with a 4% bovine serum albumin (BSA) solution, the membranes were incubated with the following primary antibodies at 4°C overnight: rabbit anti-IRF3 S386 (1:500) (ab76493; Abcam, MA, USA), rabbit anti-IRF3 S396 (1:1,000) (no. 29047; Cell Signaling Technology [CST], Danvers, MA, USA), rabbit anti-IRF3 (1:1,000) (no. 4302; CST), rabbit anti-IFIT1 no. 971 (1:1,000) and rabbit anti-MxA no. 340B (1:500) (both made at UT Southwestern Antibody Core facility by repeated injections of synthetic peptides into rabbits), mouse anti-pan-actin C4 (1:3,000) (MAB1501; Millipore), rabbit anti-ZIKV-NS5 (1:2,500) (GTX133312; GeneTex, Irvine, CA, USA), goat anti-WNV-NS3 (1:1,000) (BAF2901; R & D Systems, Minneapolis, MN, USA), goat anti-parainfluenza virus 1 (B65121G-1; Amsbio, Cambridge, MA, USA), mouse anti-RIG-I AlmeI (1:1,000) (AG-20B-0009-C100; AdipoGen, San Diego, CA, USA), rabbit anti-MDA5 (1:500) (29020; IBL, Minneapolis, MN, USA), rabbit anti-LGP2 (1:500) (no. 29030; IBL), and rabbit anti-Cardif AT107 (1:1,000) (ALX-210-929-C100; Enzo Life Sciences, Farmingdale, NY, USA). .. Bound primary antibodies were detected with 1:1,000 diluted horseradish peroxidase-coupled secondary antibodies: donkey anti-mouse antibody (715-035-150), donkey anti-rabbit antibody (711-035-152), donkey anti-goat antibody (705-035-003) (all from Jackson Immunoresearch, West Grove, PA, USA), and an ECL prime reagent (GE Healthcare, Pittsburgh, PA, USA) on a ChemiDoc Imager (Bio-Rad).

Article Title: Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling
Article Snippet: .. Following washes, the blots were incubated with IRDye 680RD donkey anti-rabbit IgG, peroxidase AffiniPure donkey anti-goat IgG, or peroxidase AffiniPure goat anti-rabbit IgG secondary antibodies, as appropriate. .. The TFAP4 and SERBP1 blots were imaged using the Li-Cor Odyssey imaging system, and the CSNK1A1 and APC blots were developed using the SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific Cat. # 34095).

Article Title: Proteomic identification of FHL1 as the protein mutated in human reducing body myopathy
Article Snippet: .. After washing, the membrane was incubated 1 hour at room temperature with HRP-conjugated secondary antibody (donkey anti-goat HRP; Jackson ImmunoResearch Laboratories; 705-035-147, 1:15,000) diluted in blocking buffer. .. Detection was performed using the ECL system by Amersham.

Article Title: Expression of nerve growth factor carried by pseudotyped lentivirus improves neuron survival and cognitive functional recovery of post‐ischemia in rats, et al. Expression of nerve growth factor carried by pseudotyped lentivirus improves neuron survival and cognitive functional recovery of post‐ischemia in rats
Article Snippet: .. The blot was washed with PBS‐T for 10 minutes, 3 times and then incubated in Peroxidase AffiniPure Donkey anti‐Goat IgG (1:5000, Jackson ImmunoResearch, West Grove, PA, USA) or in Peroxidase‐conjugated AffiniPure Goat Anti‐Rabbit IgG (H + L) at RT for 1 hour. .. After PBS‐T washing, the blot was incubated with Immobilon Western Chemiluminescent HRP substrate (EMD Millipore, Billerica, MA, USA). β‐actin was used as an internal control of each sample by incubation with HRP‐conjugated anti‐β‐actin IgG (1:5000, Proteintech Group, Chicago, USA).

Article Title: Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord
Article Snippet: After three washes in PBS/0.1% Tween 20, membranes were incubated with the appropriate HRP-conjugated secondary antibody at room temperature for 1h. .. Antibodies used: mouse anti-Ndfip1 (D-4) (1:50, Santa Cruz Biotechnology #sc-398469), mouse anti-Ndfip2 (E-4) (1:50, Santa Cruz Biotechnology #sc-376259), goat anti-Robo1 (1:500, R & D systems #AF1749), goat anti-Robo2 (1:500, R & D systems #AF3147), goat anti-Robo3 (1:1000, R & D systems #AF3076), goat anti-DCC (1:500, R & D systems #AF844) and donkey anti-goat HRP (1:10,000, Jackson Immunoresearch #705-035-003).

Article Title: Requirement of β1 integrin for endothelium-dependent vasodilation and collateral formation in hindlimb ischemia
Article Snippet: .. The membrane was incubated with rabbit anti-GAPDH (Abcam, ab9485) at 1/5,000 dilution, rabbit anti-eNOS (9572S, Cell Signaling Technologies) at 1/500 dilution, rabbit anti-Phospho(Ser1177)-eNOS (9571S, Cell Signaling Technologies) at 1/500 dilution or goat anti-β1 integrin (Santa Cruz, sc-6622) at 1/2,000 dilution as primary antibodies overnight at 4 °C, and HRP-conjugated donkey anti-rabbit (7074, Cell Signaling Technologies) at 1/2,000 dilution and donkey anti-goat (705-035-147, Jackson ImmunoResearch) at 1/5,000 dilution as secondary antibodies for 1 h at RT. .. Chemiluminescence was detected using WesternBright Quantum Kit (Advansta) and ChemiDoc MP Imaging System (Bio-Rad).

Stripping Membranes:

Article Title: Comparative Analysis of African and Asian Lineage-Derived Zika Virus Strains Reveals Differences in Activation of and Sensitivity to Antiviral Innate Immunity
Article Snippet: Bound primary antibodies were detected with 1:1,000 diluted horseradish peroxidase-coupled secondary antibodies: donkey anti-mouse antibody (715-035-150), donkey anti-rabbit antibody (711-035-152), donkey anti-goat antibody (705-035-003) (all from Jackson Immunoresearch, West Grove, PA, USA), and an ECL prime reagent (GE Healthcare, Pittsburgh, PA, USA) on a ChemiDoc Imager (Bio-Rad). .. If necessary, membranes were stripped for reprobing with a harsh stripping buffer containing 2% SDS and 0.8% β-mercaptoethanol.

Article Title: Proteomic identification of FHL1 as the protein mutated in human reducing body myopathy
Article Snippet: After washing, the membrane was incubated 1 hour at room temperature with HRP-conjugated secondary antibody (donkey anti-goat HRP; Jackson ImmunoResearch Laboratories; 705-035-147, 1:15,000) diluted in blocking buffer. .. After stripping the membrane (25 mM glycine-HCl at pH 2.0, 1% SDS) and washing in 1× PBS loading control was performed with same method using primary antibody against GAPDH (mouse monoclonal antibody; Chemicon; MAB374) and secondary antibody goat anti-mouse HRP (Santa Cruz Biotechnology Inc.; 1:3,000).

Expressing:

Article Title: Novel PDGFRB rearrangement in multifocal infantile myofibromatosis is tumorigenic and sensitive to imatinib
Article Snippet: .. Secondary antibodies included anti-goat (Jackson ImmunoResearch 705-035-003), anti-rabbit (Jackson ImmunoResearch 111-035-003), and anti-mouse (Jackson ImmunoResearch 715-035-0150, and protein expression was detected by using both CareStream film (Sigma-Aldrich Z3730398) and Bio-Rad Clarity Enhanced Chemiluminescence (Bio-Rad 1705060) .. Imatinib Sensitivity Pdgfrb −/− MEFs ectopically expressing either P-WT or P-Ex12 were plated in a 96-well plate, exposed to imatinib at doses ranging from 1 nM to 10 µM for 5 d, and analyzed by Cyquant (ThermoFisher Scientific C7026).

Article Title: Expression of nerve growth factor carried by pseudotyped lentivirus improves neuron survival and cognitive functional recovery of post‐ischemia in rats, et al. Expression of nerve growth factor carried by pseudotyped lentivirus improves neuron survival and cognitive functional recovery of post‐ischemia in rats
Article Snippet: The proteins were analyzed for the expression of the β‐NGF gene. .. The blot was washed with PBS‐T for 10 minutes, 3 times and then incubated in Peroxidase AffiniPure Donkey anti‐Goat IgG (1:5000, Jackson ImmunoResearch, West Grove, PA, USA) or in Peroxidase‐conjugated AffiniPure Goat Anti‐Rabbit IgG (H + L) at RT for 1 hour.

Article Title: Requirement of β1 integrin for endothelium-dependent vasodilation and collateral formation in hindlimb ischemia
Article Snippet: The membrane was incubated with rabbit anti-GAPDH (Abcam, ab9485) at 1/5,000 dilution, rabbit anti-eNOS (9572S, Cell Signaling Technologies) at 1/500 dilution, rabbit anti-Phospho(Ser1177)-eNOS (9571S, Cell Signaling Technologies) at 1/500 dilution or goat anti-β1 integrin (Santa Cruz, sc-6622) at 1/2,000 dilution as primary antibodies overnight at 4 °C, and HRP-conjugated donkey anti-rabbit (7074, Cell Signaling Technologies) at 1/2,000 dilution and donkey anti-goat (705-035-147, Jackson ImmunoResearch) at 1/5,000 dilution as secondary antibodies for 1 h at RT. .. For expression and phosphorylation evaluation, semi-quantitative band density analysis was performed in FIJI (ImageJ NIH), where GAPDH was used as housekeeping protein for normalization.

BIA-KA:

Article Title: Comparative Analysis of African and Asian Lineage-Derived Zika Virus Strains Reveals Differences in Activation of and Sensitivity to Antiviral Innate Immunity
Article Snippet: Protein concentrations were measured using the Pierce BCA protein assay kit (Thermo Fisher), and 12 μg protein was subjected to SDS-PAGE and blotted onto polyvinylidene difluoride (PVDF) membranes (Thermo Fisher). .. Bound primary antibodies were detected with 1:1,000 diluted horseradish peroxidase-coupled secondary antibodies: donkey anti-mouse antibody (715-035-150), donkey anti-rabbit antibody (711-035-152), donkey anti-goat antibody (705-035-003) (all from Jackson Immunoresearch, West Grove, PA, USA), and an ECL prime reagent (GE Healthcare, Pittsburgh, PA, USA) on a ChemiDoc Imager (Bio-Rad).

Article Title: Expression of nerve growth factor carried by pseudotyped lentivirus improves neuron survival and cognitive functional recovery of post‐ischemia in rats, et al. Expression of nerve growth factor carried by pseudotyped lentivirus improves neuron survival and cognitive functional recovery of post‐ischemia in rats
Article Snippet: The BCA protein assay kit was used to measure the protein concentration of the supernatant. .. The blot was washed with PBS‐T for 10 minutes, 3 times and then incubated in Peroxidase AffiniPure Donkey anti‐Goat IgG (1:5000, Jackson ImmunoResearch, West Grove, PA, USA) or in Peroxidase‐conjugated AffiniPure Goat Anti‐Rabbit IgG (H + L) at RT for 1 hour.

Modification:

Article Title: Comparative Analysis of African and Asian Lineage-Derived Zika Virus Strains Reveals Differences in Activation of and Sensitivity to Antiviral Innate Immunity
Article Snippet: Cells were lysed in a modified radioimmunoprecipitation (RIPA) buffer (150 mM NaCl, 50 mM Tris-HCl [pH 7.6], 1% Triton X-100, 0.5% sodium deoxycholate) with freshly added protease inhibitor cocktail (Sigma, St. Louis, MO, USA), phosphatase inhibitor cocktail (Millipore, Burlington, MA, USA), and okadaic acid (Thermo Fisher, Waltham, MA, USA). .. Bound primary antibodies were detected with 1:1,000 diluted horseradish peroxidase-coupled secondary antibodies: donkey anti-mouse antibody (715-035-150), donkey anti-rabbit antibody (711-035-152), donkey anti-goat antibody (705-035-003) (all from Jackson Immunoresearch, West Grove, PA, USA), and an ECL prime reagent (GE Healthcare, Pittsburgh, PA, USA) on a ChemiDoc Imager (Bio-Rad).

Western Blot:

Article Title: Comparative Analysis of African and Asian Lineage-Derived Zika Virus Strains Reveals Differences in Activation of and Sensitivity to Antiviral Innate Immunity
Article Snippet: Paragraph title: SDS-PAGE and Western blot analysis. ... Bound primary antibodies were detected with 1:1,000 diluted horseradish peroxidase-coupled secondary antibodies: donkey anti-mouse antibody (715-035-150), donkey anti-rabbit antibody (711-035-152), donkey anti-goat antibody (705-035-003) (all from Jackson Immunoresearch, West Grove, PA, USA), and an ECL prime reagent (GE Healthcare, Pittsburgh, PA, USA) on a ChemiDoc Imager (Bio-Rad).

Article Title: Novel PDGFRB rearrangement in multifocal infantile myofibromatosis is tumorigenic and sensitive to imatinib
Article Snippet: For western blotting, proteins were solubilized in RIPA lysis buffer supplemented with PhosStop Phosphotase inhibitor and Roche Complete Protease inhibitor (Roche). .. Secondary antibodies included anti-goat (Jackson ImmunoResearch 705-035-003), anti-rabbit (Jackson ImmunoResearch 111-035-003), and anti-mouse (Jackson ImmunoResearch 715-035-0150, and protein expression was detected by using both CareStream film (Sigma-Aldrich Z3730398) and Bio-Rad Clarity Enhanced Chemiluminescence (Bio-Rad 1705060)

Article Title: Proteomic identification of FHL1 as the protein mutated in human reducing body myopathy
Article Snippet: Paragraph title: Western blotting. ... After washing, the membrane was incubated 1 hour at room temperature with HRP-conjugated secondary antibody (donkey anti-goat HRP; Jackson ImmunoResearch Laboratories; 705-035-147, 1:15,000) diluted in blocking buffer.

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: .. The membrane was blocked with 3% (w/v) BSA in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 0.1% Tween 20 (TBST), probed with goat anti-TLR2 polyclonal antibody (AF2616, R & D Systems) overnight at 4 °C, followed by peroxidase-conjugated donkey anti-rabbit antibody (705-035-147, Jackson ImmunoResearch) at room temperature for 1 h and further with Western Lightning ECL Pro (PerkinElmer Life Sciences). .. Protein bands were visualized with an ImageQuant LAS4000 Mini Biomolecular Imager (GE Healthcare).

Article Title: Expression of nerve growth factor carried by pseudotyped lentivirus improves neuron survival and cognitive functional recovery of post‐ischemia in rats, et al. Expression of nerve growth factor carried by pseudotyped lentivirus improves neuron survival and cognitive functional recovery of post‐ischemia in rats
Article Snippet: Paragraph title: 2.5. Immunohistochemistry and Western blotting ... The blot was washed with PBS‐T for 10 minutes, 3 times and then incubated in Peroxidase AffiniPure Donkey anti‐Goat IgG (1:5000, Jackson ImmunoResearch, West Grove, PA, USA) or in Peroxidase‐conjugated AffiniPure Goat Anti‐Rabbit IgG (H + L) at RT for 1 hour.

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: .. The membrane was blocked with 3% (w/v) BSA in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 0.1% Tween 20 (TBST), probed with goat anti-TLR2 polyclonal antibody (AF2616, R & D Systems) overnight at 4 °C, followed by peroxidase-conjugated donkey anti-rabbit antibody (705-035-147, Jackson ImmunoResearch) at room temperature for 1 h and further with Western Lightning ECL Pro (PerkinElmer Life Sciences). .. Protein bands were visualized with an ImageQuant LAS4000 Mini Biomolecular Imager (GE Healthcare).

Article Title: Requirement of β1 integrin for endothelium-dependent vasodilation and collateral formation in hindlimb ischemia
Article Snippet: Paragraph title: Western blotting ... The membrane was incubated with rabbit anti-GAPDH (Abcam, ab9485) at 1/5,000 dilution, rabbit anti-eNOS (9572S, Cell Signaling Technologies) at 1/500 dilution, rabbit anti-Phospho(Ser1177)-eNOS (9571S, Cell Signaling Technologies) at 1/500 dilution or goat anti-β1 integrin (Santa Cruz, sc-6622) at 1/2,000 dilution as primary antibodies overnight at 4 °C, and HRP-conjugated donkey anti-rabbit (7074, Cell Signaling Technologies) at 1/2,000 dilution and donkey anti-goat (705-035-147, Jackson ImmunoResearch) at 1/5,000 dilution as secondary antibodies for 1 h at RT.

Hybridization:

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: Proteins were transferred to polyvinylfluoride membrane (PolyScreen PVDF Hybridization Transfer Membrane; PerkinElmer Life Sciences) by semi-dry blotting using Towbin buffer (15 V, 60 min). .. The membrane was blocked with 3% (w/v) BSA in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 0.1% Tween 20 (TBST), probed with goat anti-TLR2 polyclonal antibody (AF2616, R & D Systems) overnight at 4 °C, followed by peroxidase-conjugated donkey anti-rabbit antibody (705-035-147, Jackson ImmunoResearch) at room temperature for 1 h and further with Western Lightning ECL Pro (PerkinElmer Life Sciences).

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: Proteins were transferred to polyvinylfluoride membrane (PolyScreen PVDF Hybridization Transfer Membrane; PerkinElmer Life Sciences) by semi-dry blotting using Towbin buffer (15 V, 60 min). .. The membrane was blocked with 3% (w/v) BSA in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 0.1% Tween 20 (TBST), probed with goat anti-TLR2 polyclonal antibody (AF2616, R & D Systems) overnight at 4 °C, followed by peroxidase-conjugated donkey anti-rabbit antibody (705-035-147, Jackson ImmunoResearch) at room temperature for 1 h and further with Western Lightning ECL Pro (PerkinElmer Life Sciences).

Flow Cytometry:

Article Title: Platelet Surface-Associated Activation and Secretion-Mediated Inhibition of Coagulation Factor XII
Article Snippet: .. Materials The following materials were used: thrombin (Haematologic Technologies; Essex Junction, VT, USA); fXIIa and fXII (Enzyme Research Laboratories; South Bend, IN, USA); AlignFlow flow cytometry alignment beads (2.5 μm for 488-nm excitation), fluorescein-5-isothiocyanate (FITC), and tetramethylrhodamine (TMRM) (Molecular Probes; Eugene, OR, USA); unlabelled and FITC-annexin V (BD Biosciences; San Jose, CA, USA); AlexaFluor-647-annexin V (Biolegend; San Diego, CA, USA); prostaglandin E1 (PGE1) (MP Biochemicals; Irvine, CA, USA); PPACK (EMD Chemicals; Gibbstown, NJ, USA); the chromomeric substrates S2238 and S2302 (Chromogenix; Milano, Italy); HEPES, bovine serum albumin, Sepharose CL-2B, Protein G sepharose, apyrase grade VII, mepacrine (quinacrine), PBS, EDTA and DMSO (Sigma-Aldrich; St Louis, MO, USA); calpeptin and MDL 28170 (Tocris Bioscience; Ellisville, MO, USA); G1/C1-inhibitor and polyclonal goat anti-human serpin G1/C1-inhibitor antibody (R & D Systems; Minneapolis, MN, USA); goat polyclonal anti-human factor XII antibody (LifeSpan BioSciences, Inc., Seattle, WA, USA); peroxidase-AffiniPure donkey anti-goat IgG (Jackson ImmunoResearch Laboratories; West Grove, PA, USA); egg phosphatidylcholine and egg phosphatidylserine (Avanti Polar Lipids; Alabaster, AL, USA); DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) (AppliChem; Darmstadt, Germany); and a kit to estimate fXII activation (Renam; Moscow, Russia). ..

Immunohistochemistry:

Article Title: Expression of nerve growth factor carried by pseudotyped lentivirus improves neuron survival and cognitive functional recovery of post‐ischemia in rats, et al. Expression of nerve growth factor carried by pseudotyped lentivirus improves neuron survival and cognitive functional recovery of post‐ischemia in rats
Article Snippet: Paragraph title: 2.5. Immunohistochemistry and Western blotting ... The blot was washed with PBS‐T for 10 minutes, 3 times and then incubated in Peroxidase AffiniPure Donkey anti‐Goat IgG (1:5000, Jackson ImmunoResearch, West Grove, PA, USA) or in Peroxidase‐conjugated AffiniPure Goat Anti‐Rabbit IgG (H + L) at RT for 1 hour.

Activation Assay:

Article Title: Platelet Surface-Associated Activation and Secretion-Mediated Inhibition of Coagulation Factor XII
Article Snippet: .. Materials The following materials were used: thrombin (Haematologic Technologies; Essex Junction, VT, USA); fXIIa and fXII (Enzyme Research Laboratories; South Bend, IN, USA); AlignFlow flow cytometry alignment beads (2.5 μm for 488-nm excitation), fluorescein-5-isothiocyanate (FITC), and tetramethylrhodamine (TMRM) (Molecular Probes; Eugene, OR, USA); unlabelled and FITC-annexin V (BD Biosciences; San Jose, CA, USA); AlexaFluor-647-annexin V (Biolegend; San Diego, CA, USA); prostaglandin E1 (PGE1) (MP Biochemicals; Irvine, CA, USA); PPACK (EMD Chemicals; Gibbstown, NJ, USA); the chromomeric substrates S2238 and S2302 (Chromogenix; Milano, Italy); HEPES, bovine serum albumin, Sepharose CL-2B, Protein G sepharose, apyrase grade VII, mepacrine (quinacrine), PBS, EDTA and DMSO (Sigma-Aldrich; St Louis, MO, USA); calpeptin and MDL 28170 (Tocris Bioscience; Ellisville, MO, USA); G1/C1-inhibitor and polyclonal goat anti-human serpin G1/C1-inhibitor antibody (R & D Systems; Minneapolis, MN, USA); goat polyclonal anti-human factor XII antibody (LifeSpan BioSciences, Inc., Seattle, WA, USA); peroxidase-AffiniPure donkey anti-goat IgG (Jackson ImmunoResearch Laboratories; West Grove, PA, USA); egg phosphatidylcholine and egg phosphatidylserine (Avanti Polar Lipids; Alabaster, AL, USA); DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) (AppliChem; Darmstadt, Germany); and a kit to estimate fXII activation (Renam; Moscow, Russia). ..

Protease Inhibitor:

Article Title: Comparative Analysis of African and Asian Lineage-Derived Zika Virus Strains Reveals Differences in Activation of and Sensitivity to Antiviral Innate Immunity
Article Snippet: Cells were lysed in a modified radioimmunoprecipitation (RIPA) buffer (150 mM NaCl, 50 mM Tris-HCl [pH 7.6], 1% Triton X-100, 0.5% sodium deoxycholate) with freshly added protease inhibitor cocktail (Sigma, St. Louis, MO, USA), phosphatase inhibitor cocktail (Millipore, Burlington, MA, USA), and okadaic acid (Thermo Fisher, Waltham, MA, USA). .. Bound primary antibodies were detected with 1:1,000 diluted horseradish peroxidase-coupled secondary antibodies: donkey anti-mouse antibody (715-035-150), donkey anti-rabbit antibody (711-035-152), donkey anti-goat antibody (705-035-003) (all from Jackson Immunoresearch, West Grove, PA, USA), and an ECL prime reagent (GE Healthcare, Pittsburgh, PA, USA) on a ChemiDoc Imager (Bio-Rad).

Article Title: Novel PDGFRB rearrangement in multifocal infantile myofibromatosis is tumorigenic and sensitive to imatinib
Article Snippet: For western blotting, proteins were solubilized in RIPA lysis buffer supplemented with PhosStop Phosphotase inhibitor and Roche Complete Protease inhibitor (Roche). .. Secondary antibodies included anti-goat (Jackson ImmunoResearch 705-035-003), anti-rabbit (Jackson ImmunoResearch 111-035-003), and anti-mouse (Jackson ImmunoResearch 715-035-0150, and protein expression was detected by using both CareStream film (Sigma-Aldrich Z3730398) and Bio-Rad Clarity Enhanced Chemiluminescence (Bio-Rad 1705060)

Article Title: Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord
Article Snippet: For preparation of mouse brain and spinal cord lysates, wild-type and Ndfip1 KO mice were anesthetized and whole brain and spinal cord were dissected and lysed in TBS supplemented with 1% Triton X-100, Complete Protease Inhibitor, and 1 mM PMSF by using a dounce homogenizer. .. Antibodies used: mouse anti-Ndfip1 (D-4) (1:50, Santa Cruz Biotechnology #sc-398469), mouse anti-Ndfip2 (E-4) (1:50, Santa Cruz Biotechnology #sc-376259), goat anti-Robo1 (1:500, R & D systems #AF1749), goat anti-Robo2 (1:500, R & D systems #AF3147), goat anti-Robo3 (1:1000, R & D systems #AF3076), goat anti-DCC (1:500, R & D systems #AF844) and donkey anti-goat HRP (1:10,000, Jackson Immunoresearch #705-035-003).

Article Title: Requirement of β1 integrin for endothelium-dependent vasodilation and collateral formation in hindlimb ischemia
Article Snippet: Western blotting Cultured and transfected HCAEC were lysed in RIPA buffer (50 mM Tris/HCl pH 7.4, 150 mM NaCl, 1% IGEPAL, 0.25% Na-deoxycholate, 1 mM EDTA) with protease inhibitors (cOmplete Protease Inhibitor Cocktail Tablets, Roche) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail, Roche). .. The membrane was incubated with rabbit anti-GAPDH (Abcam, ab9485) at 1/5,000 dilution, rabbit anti-eNOS (9572S, Cell Signaling Technologies) at 1/500 dilution, rabbit anti-Phospho(Ser1177)-eNOS (9571S, Cell Signaling Technologies) at 1/500 dilution or goat anti-β1 integrin (Santa Cruz, sc-6622) at 1/2,000 dilution as primary antibodies overnight at 4 °C, and HRP-conjugated donkey anti-rabbit (7074, Cell Signaling Technologies) at 1/2,000 dilution and donkey anti-goat (705-035-147, Jackson ImmunoResearch) at 1/5,000 dilution as secondary antibodies for 1 h at RT.

Cell Culture:

Article Title: Novel PDGFRB rearrangement in multifocal infantile myofibromatosis is tumorigenic and sensitive to imatinib
Article Snippet: Ectopic mRNA and Protein Expression Pdgfrb −/− MEFs or 10T1/2 fibroblasts were transduced, sorted by FACS analysis for GFP expression, and replated onto standard cell culture plates. .. Secondary antibodies included anti-goat (Jackson ImmunoResearch 705-035-003), anti-rabbit (Jackson ImmunoResearch 111-035-003), and anti-mouse (Jackson ImmunoResearch 715-035-0150, and protein expression was detected by using both CareStream film (Sigma-Aldrich Z3730398) and Bio-Rad Clarity Enhanced Chemiluminescence (Bio-Rad 1705060)

Article Title: Requirement of β1 integrin for endothelium-dependent vasodilation and collateral formation in hindlimb ischemia
Article Snippet: Western blotting Cultured and transfected HCAEC were lysed in RIPA buffer (50 mM Tris/HCl pH 7.4, 150 mM NaCl, 1% IGEPAL, 0.25% Na-deoxycholate, 1 mM EDTA) with protease inhibitors (cOmplete Protease Inhibitor Cocktail Tablets, Roche) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail, Roche). .. The membrane was incubated with rabbit anti-GAPDH (Abcam, ab9485) at 1/5,000 dilution, rabbit anti-eNOS (9572S, Cell Signaling Technologies) at 1/500 dilution, rabbit anti-Phospho(Ser1177)-eNOS (9571S, Cell Signaling Technologies) at 1/500 dilution or goat anti-β1 integrin (Santa Cruz, sc-6622) at 1/2,000 dilution as primary antibodies overnight at 4 °C, and HRP-conjugated donkey anti-rabbit (7074, Cell Signaling Technologies) at 1/2,000 dilution and donkey anti-goat (705-035-147, Jackson ImmunoResearch) at 1/5,000 dilution as secondary antibodies for 1 h at RT.

Imaging:

Article Title: Requirement of β1 integrin for endothelium-dependent vasodilation and collateral formation in hindlimb ischemia
Article Snippet: The membrane was incubated with rabbit anti-GAPDH (Abcam, ab9485) at 1/5,000 dilution, rabbit anti-eNOS (9572S, Cell Signaling Technologies) at 1/500 dilution, rabbit anti-Phospho(Ser1177)-eNOS (9571S, Cell Signaling Technologies) at 1/500 dilution or goat anti-β1 integrin (Santa Cruz, sc-6622) at 1/2,000 dilution as primary antibodies overnight at 4 °C, and HRP-conjugated donkey anti-rabbit (7074, Cell Signaling Technologies) at 1/2,000 dilution and donkey anti-goat (705-035-147, Jackson ImmunoResearch) at 1/5,000 dilution as secondary antibodies for 1 h at RT. .. Chemiluminescence was detected using WesternBright Quantum Kit (Advansta) and ChemiDoc MP Imaging System (Bio-Rad).

Protein Concentration:

Article Title: Expression of nerve growth factor carried by pseudotyped lentivirus improves neuron survival and cognitive functional recovery of post‐ischemia in rats, et al. Expression of nerve growth factor carried by pseudotyped lentivirus improves neuron survival and cognitive functional recovery of post‐ischemia in rats
Article Snippet: The BCA protein assay kit was used to measure the protein concentration of the supernatant. .. The blot was washed with PBS‐T for 10 minutes, 3 times and then incubated in Peroxidase AffiniPure Donkey anti‐Goat IgG (1:5000, Jackson ImmunoResearch, West Grove, PA, USA) or in Peroxidase‐conjugated AffiniPure Goat Anti‐Rabbit IgG (H + L) at RT for 1 hour.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Novel PDGFRB rearrangement in multifocal infantile myofibromatosis is tumorigenic and sensitive to imatinib
Article Snippet: RT-PCR primers were designed using the National Library of Medicine PRIMER-BLAST tool, and obtained from Sigma-Aldrich. .. Secondary antibodies included anti-goat (Jackson ImmunoResearch 705-035-003), anti-rabbit (Jackson ImmunoResearch 111-035-003), and anti-mouse (Jackson ImmunoResearch 715-035-0150, and protein expression was detected by using both CareStream film (Sigma-Aldrich Z3730398) and Bio-Rad Clarity Enhanced Chemiluminescence (Bio-Rad 1705060)

Sonication:

Article Title: Proteomic identification of FHL1 as the protein mutated in human reducing body myopathy
Article Snippet: After lysis (10% SDS, 10 mM EDTA, 10 mM EGTA, 50 mM DTT, 6% glycerol, 60 mM Tris-HCl) and sonication, 0.5 mg of muscle tissue in loading buffer (Laemmli Sample Buffer; Bio-Rad; 5% β-mercaptoethanol) were submitted to electrophoresis on a 10% SDS-PAGE gel and then transferred onto a PVDF membrane (Millipore). .. After washing, the membrane was incubated 1 hour at room temperature with HRP-conjugated secondary antibody (donkey anti-goat HRP; Jackson ImmunoResearch Laboratories; 705-035-147, 1:15,000) diluted in blocking buffer.

Affinity Purification:

Article Title: Cooperative Control via Lymphoid Enhancer Factor 1/T Cell Factor 3 and Estrogen Receptor-? for Uterine Gene Regulation by Estrogen
Article Snippet: The affinity-purified polyclonal antibodies for Bip (catalog no. sc-1050), ERα (catalog no. sc-542), actin (catalog no.sc-1615), PR (catalog no. sc-539), Lef-1 (catalog no. sc-8592), Tcf-3 (catalog no. sc-13026) and Wnt-4 (catalog no. sc-5215) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). .. Secondary antibodies for fluorescein isothiocyanate (FITC)-conjugated donkey antigoat (catalog no. 705-095-147), Texas red-conjugated donkey antirabbit (catalog no. 711-075-152), and peroxidase-conjugated donkey antimouse (catalog no. 715-035-150), donkey antirabbit (catalog no. 711-035-152), or donkey antigoat (catalog no. 705-035-147) were purchased from the Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA).

Fluorescence:

Article Title: Zika virus degrades the ω-3 fatty acid transporter Mfsd2a in brain microvascular endothelial cells and impairs lipid homeostasis
Article Snippet: .. Fluorescence (111-545-003) or horseradish peroxidase (HRP)–conjugated secondary antibodies (111-035-003, 115-035-003, and 705-035-003) were obtained from Jackson ImmunoResearch (West Grove, PA, USA). ..

Magnetic Beads:

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: The supernatant after the removal of magnetic beads was subjected to SDS-PAGE using the Laemmli buffer system. .. The membrane was blocked with 3% (w/v) BSA in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 0.1% Tween 20 (TBST), probed with goat anti-TLR2 polyclonal antibody (AF2616, R & D Systems) overnight at 4 °C, followed by peroxidase-conjugated donkey anti-rabbit antibody (705-035-147, Jackson ImmunoResearch) at room temperature for 1 h and further with Western Lightning ECL Pro (PerkinElmer Life Sciences).

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: The supernatant after the removal of magnetic beads was subjected to SDS-PAGE using the Laemmli buffer system. .. The membrane was blocked with 3% (w/v) BSA in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 0.1% Tween 20 (TBST), probed with goat anti-TLR2 polyclonal antibody (AF2616, R & D Systems) overnight at 4 °C, followed by peroxidase-conjugated donkey anti-rabbit antibody (705-035-147, Jackson ImmunoResearch) at room temperature for 1 h and further with Western Lightning ECL Pro (PerkinElmer Life Sciences).

Transfection:

Article Title: Requirement of β1 integrin for endothelium-dependent vasodilation and collateral formation in hindlimb ischemia
Article Snippet: Western blotting Cultured and transfected HCAEC were lysed in RIPA buffer (50 mM Tris/HCl pH 7.4, 150 mM NaCl, 1% IGEPAL, 0.25% Na-deoxycholate, 1 mM EDTA) with protease inhibitors (cOmplete Protease Inhibitor Cocktail Tablets, Roche) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail, Roche). .. The membrane was incubated with rabbit anti-GAPDH (Abcam, ab9485) at 1/5,000 dilution, rabbit anti-eNOS (9572S, Cell Signaling Technologies) at 1/500 dilution, rabbit anti-Phospho(Ser1177)-eNOS (9571S, Cell Signaling Technologies) at 1/500 dilution or goat anti-β1 integrin (Santa Cruz, sc-6622) at 1/2,000 dilution as primary antibodies overnight at 4 °C, and HRP-conjugated donkey anti-rabbit (7074, Cell Signaling Technologies) at 1/2,000 dilution and donkey anti-goat (705-035-147, Jackson ImmunoResearch) at 1/5,000 dilution as secondary antibodies for 1 h at RT.

Mouse Assay:

Article Title: Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord
Article Snippet: For preparation of mouse brain and spinal cord lysates, wild-type and Ndfip1 KO mice were anesthetized and whole brain and spinal cord were dissected and lysed in TBS supplemented with 1% Triton X-100, Complete Protease Inhibitor, and 1 mM PMSF by using a dounce homogenizer. .. Antibodies used: mouse anti-Ndfip1 (D-4) (1:50, Santa Cruz Biotechnology #sc-398469), mouse anti-Ndfip2 (E-4) (1:50, Santa Cruz Biotechnology #sc-376259), goat anti-Robo1 (1:500, R & D systems #AF1749), goat anti-Robo2 (1:500, R & D systems #AF3147), goat anti-Robo3 (1:1000, R & D systems #AF3076), goat anti-DCC (1:500, R & D systems #AF844) and donkey anti-goat HRP (1:10,000, Jackson Immunoresearch #705-035-003).

Quantitative RT-PCR:

Article Title: Novel PDGFRB rearrangement in multifocal infantile myofibromatosis is tumorigenic and sensitive to imatinib
Article Snippet: Expression of P-WT and P-Ex12 was assessed by qRT-PCR or western blotting, essentially as previously described ( ). .. Secondary antibodies included anti-goat (Jackson ImmunoResearch 705-035-003), anti-rabbit (Jackson ImmunoResearch 111-035-003), and anti-mouse (Jackson ImmunoResearch 715-035-0150, and protein expression was detected by using both CareStream film (Sigma-Aldrich Z3730398) and Bio-Rad Clarity Enhanced Chemiluminescence (Bio-Rad 1705060)

FACS:

Article Title: Novel PDGFRB rearrangement in multifocal infantile myofibromatosis is tumorigenic and sensitive to imatinib
Article Snippet: Ectopic mRNA and Protein Expression Pdgfrb −/− MEFs or 10T1/2 fibroblasts were transduced, sorted by FACS analysis for GFP expression, and replated onto standard cell culture plates. .. Secondary antibodies included anti-goat (Jackson ImmunoResearch 705-035-003), anti-rabbit (Jackson ImmunoResearch 111-035-003), and anti-mouse (Jackson ImmunoResearch 715-035-0150, and protein expression was detected by using both CareStream film (Sigma-Aldrich Z3730398) and Bio-Rad Clarity Enhanced Chemiluminescence (Bio-Rad 1705060)

SDS Page:

Article Title: Comparative Analysis of African and Asian Lineage-Derived Zika Virus Strains Reveals Differences in Activation of and Sensitivity to Antiviral Innate Immunity
Article Snippet: Paragraph title: SDS-PAGE and Western blot analysis. ... Bound primary antibodies were detected with 1:1,000 diluted horseradish peroxidase-coupled secondary antibodies: donkey anti-mouse antibody (715-035-150), donkey anti-rabbit antibody (711-035-152), donkey anti-goat antibody (705-035-003) (all from Jackson Immunoresearch, West Grove, PA, USA), and an ECL prime reagent (GE Healthcare, Pittsburgh, PA, USA) on a ChemiDoc Imager (Bio-Rad).

Article Title: Proteomic identification of FHL1 as the protein mutated in human reducing body myopathy
Article Snippet: After lysis (10% SDS, 10 mM EDTA, 10 mM EGTA, 50 mM DTT, 6% glycerol, 60 mM Tris-HCl) and sonication, 0.5 mg of muscle tissue in loading buffer (Laemmli Sample Buffer; Bio-Rad; 5% β-mercaptoethanol) were submitted to electrophoresis on a 10% SDS-PAGE gel and then transferred onto a PVDF membrane (Millipore). .. After washing, the membrane was incubated 1 hour at room temperature with HRP-conjugated secondary antibody (donkey anti-goat HRP; Jackson ImmunoResearch Laboratories; 705-035-147, 1:15,000) diluted in blocking buffer.

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: The supernatant after the removal of magnetic beads was subjected to SDS-PAGE using the Laemmli buffer system. .. The membrane was blocked with 3% (w/v) BSA in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 0.1% Tween 20 (TBST), probed with goat anti-TLR2 polyclonal antibody (AF2616, R & D Systems) overnight at 4 °C, followed by peroxidase-conjugated donkey anti-rabbit antibody (705-035-147, Jackson ImmunoResearch) at room temperature for 1 h and further with Western Lightning ECL Pro (PerkinElmer Life Sciences).

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: The supernatant after the removal of magnetic beads was subjected to SDS-PAGE using the Laemmli buffer system. .. The membrane was blocked with 3% (w/v) BSA in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 0.1% Tween 20 (TBST), probed with goat anti-TLR2 polyclonal antibody (AF2616, R & D Systems) overnight at 4 °C, followed by peroxidase-conjugated donkey anti-rabbit antibody (705-035-147, Jackson ImmunoResearch) at room temperature for 1 h and further with Western Lightning ECL Pro (PerkinElmer Life Sciences).

Article Title: Requirement of β1 integrin for endothelium-dependent vasodilation and collateral formation in hindlimb ischemia
Article Snippet: The lysates were heated in Laemmli buffer (Bio-Rad) containing β-mercaptoethanol (Roth) at 95 °C for 5 min. For SDS-PAGE, 4–15% SDS-gels (Bio-Rad) and Mini-PROTEAN Tetra Cell system (Bio-Rad) were used. .. The membrane was incubated with rabbit anti-GAPDH (Abcam, ab9485) at 1/5,000 dilution, rabbit anti-eNOS (9572S, Cell Signaling Technologies) at 1/500 dilution, rabbit anti-Phospho(Ser1177)-eNOS (9571S, Cell Signaling Technologies) at 1/500 dilution or goat anti-β1 integrin (Santa Cruz, sc-6622) at 1/2,000 dilution as primary antibodies overnight at 4 °C, and HRP-conjugated donkey anti-rabbit (7074, Cell Signaling Technologies) at 1/2,000 dilution and donkey anti-goat (705-035-147, Jackson ImmunoResearch) at 1/5,000 dilution as secondary antibodies for 1 h at RT.

Software:

Article Title: Proteomic identification of FHL1 as the protein mutated in human reducing body myopathy
Article Snippet: After washing, the membrane was incubated 1 hour at room temperature with HRP-conjugated secondary antibody (donkey anti-goat HRP; Jackson ImmunoResearch Laboratories; 705-035-147, 1:15,000) diluted in blocking buffer. .. Western blot band quantification was performed with ImageJ software version 1.38x (NIH).

Semi Dry Blot:

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: Proteins were transferred to polyvinylfluoride membrane (PolyScreen PVDF Hybridization Transfer Membrane; PerkinElmer Life Sciences) by semi-dry blotting using Towbin buffer (15 V, 60 min). .. The membrane was blocked with 3% (w/v) BSA in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 0.1% Tween 20 (TBST), probed with goat anti-TLR2 polyclonal antibody (AF2616, R & D Systems) overnight at 4 °C, followed by peroxidase-conjugated donkey anti-rabbit antibody (705-035-147, Jackson ImmunoResearch) at room temperature for 1 h and further with Western Lightning ECL Pro (PerkinElmer Life Sciences).

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: Proteins were transferred to polyvinylfluoride membrane (PolyScreen PVDF Hybridization Transfer Membrane; PerkinElmer Life Sciences) by semi-dry blotting using Towbin buffer (15 V, 60 min). .. The membrane was blocked with 3% (w/v) BSA in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 0.1% Tween 20 (TBST), probed with goat anti-TLR2 polyclonal antibody (AF2616, R & D Systems) overnight at 4 °C, followed by peroxidase-conjugated donkey anti-rabbit antibody (705-035-147, Jackson ImmunoResearch) at room temperature for 1 h and further with Western Lightning ECL Pro (PerkinElmer Life Sciences).

Immunoprecipitation:

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: Cell lysate (0.75 mg) was subjected to immunoprecipitation with biotinylated anti-Siglec-5/14 antibody (6 μg; clone 194128; R & D Systems) and Dynabeads MyOne Streptavidin C1 beads (30 μl) at 4 °C overnight. .. The membrane was blocked with 3% (w/v) BSA in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 0.1% Tween 20 (TBST), probed with goat anti-TLR2 polyclonal antibody (AF2616, R & D Systems) overnight at 4 °C, followed by peroxidase-conjugated donkey anti-rabbit antibody (705-035-147, Jackson ImmunoResearch) at room temperature for 1 h and further with Western Lightning ECL Pro (PerkinElmer Life Sciences).

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: Cell lysate (0.75 mg) was subjected to immunoprecipitation with biotinylated anti-Siglec-5/14 antibody (6 μg; clone 194128; R & D Systems) and Dynabeads MyOne Streptavidin C1 beads (30 μl) at 4 °C overnight. .. The membrane was blocked with 3% (w/v) BSA in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 0.1% Tween 20 (TBST), probed with goat anti-TLR2 polyclonal antibody (AF2616, R & D Systems) overnight at 4 °C, followed by peroxidase-conjugated donkey anti-rabbit antibody (705-035-147, Jackson ImmunoResearch) at room temperature for 1 h and further with Western Lightning ECL Pro (PerkinElmer Life Sciences).

Article Title: Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord
Article Snippet: Paragraph title: Immunoprecipitation ... Antibodies used: mouse anti-Ndfip1 (D-4) (1:50, Santa Cruz Biotechnology #sc-398469), mouse anti-Ndfip2 (E-4) (1:50, Santa Cruz Biotechnology #sc-376259), goat anti-Robo1 (1:500, R & D systems #AF1749), goat anti-Robo2 (1:500, R & D systems #AF3147), goat anti-Robo3 (1:1000, R & D systems #AF3076), goat anti-DCC (1:500, R & D systems #AF844) and donkey anti-goat HRP (1:10,000, Jackson Immunoresearch #705-035-003).

FLAG-tag:

Article Title: Zika virus degrades the ω-3 fatty acid transporter Mfsd2a in brain microvascular endothelial cells and impairs lipid homeostasis
Article Snippet: Antibodies and reagents Antibodies were obtained from Abcam (Cambridge, MA, USA) for Mfsd2a (ab105399) and CD31 (ab28364); BioFront Technologies (Tallahassee, FL, USA) for ZIKV E (1176-56) and ZIKV non-structural protein 1 (NS1) (1225-36); Sigma-Aldrich (St. Louis, MO, USA) for Flag-tag (F3165) and β-actin (A5441); Abmart (Shanghai, China) for GFP (M20004M); Santa Cruz Biotechnology (Santa Cruz, CA, USA) for ubiquitin (sc-8017); J. McKeating for HCV E2; H. Greenberg for HCV E1; and Thermo Fisher Scientific (Rockford, IL, USA) for His-tag (372900), Myc-tag (MA1-21316), and HA-tag (326700). .. Fluorescence (111-545-003) or horseradish peroxidase (HRP)–conjugated secondary antibodies (111-035-003, 115-035-003, and 705-035-003) were obtained from Jackson ImmunoResearch (West Grove, PA, USA).

Lysis:

Article Title: Novel PDGFRB rearrangement in multifocal infantile myofibromatosis is tumorigenic and sensitive to imatinib
Article Snippet: For western blotting, proteins were solubilized in RIPA lysis buffer supplemented with PhosStop Phosphotase inhibitor and Roche Complete Protease inhibitor (Roche). .. Secondary antibodies included anti-goat (Jackson ImmunoResearch 705-035-003), anti-rabbit (Jackson ImmunoResearch 111-035-003), and anti-mouse (Jackson ImmunoResearch 715-035-0150, and protein expression was detected by using both CareStream film (Sigma-Aldrich Z3730398) and Bio-Rad Clarity Enhanced Chemiluminescence (Bio-Rad 1705060)

Article Title: Proteomic identification of FHL1 as the protein mutated in human reducing body myopathy
Article Snippet: After lysis (10% SDS, 10 mM EDTA, 10 mM EGTA, 50 mM DTT, 6% glycerol, 60 mM Tris-HCl) and sonication, 0.5 mg of muscle tissue in loading buffer (Laemmli Sample Buffer; Bio-Rad; 5% β-mercaptoethanol) were submitted to electrophoresis on a 10% SDS-PAGE gel and then transferred onto a PVDF membrane (Millipore). .. After washing, the membrane was incubated 1 hour at room temperature with HRP-conjugated secondary antibody (donkey anti-goat HRP; Jackson ImmunoResearch Laboratories; 705-035-147, 1:15,000) diluted in blocking buffer.

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: The cells were lysed in 1 ml of lysis buffer (150 m m NaCl, 1% Nonidet P-40, and 50 m m Tris-HCl (pH 8.0)). .. The membrane was blocked with 3% (w/v) BSA in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 0.1% Tween 20 (TBST), probed with goat anti-TLR2 polyclonal antibody (AF2616, R & D Systems) overnight at 4 °C, followed by peroxidase-conjugated donkey anti-rabbit antibody (705-035-147, Jackson ImmunoResearch) at room temperature for 1 h and further with Western Lightning ECL Pro (PerkinElmer Life Sciences).

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: The cells were lysed in 1 ml of lysis buffer (150 m m NaCl, 1% Nonidet P-40, and 50 m m Tris-HCl (pH 8.0)). .. The membrane was blocked with 3% (w/v) BSA in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, and 0.1% Tween 20 (TBST), probed with goat anti-TLR2 polyclonal antibody (AF2616, R & D Systems) overnight at 4 °C, followed by peroxidase-conjugated donkey anti-rabbit antibody (705-035-147, Jackson ImmunoResearch) at room temperature for 1 h and further with Western Lightning ECL Pro (PerkinElmer Life Sciences).

Article Title: Requirement of β1 integrin for endothelium-dependent vasodilation and collateral formation in hindlimb ischemia
Article Snippet: After lysis, fresh samples were directly used for protein analysis. .. The membrane was incubated with rabbit anti-GAPDH (Abcam, ab9485) at 1/5,000 dilution, rabbit anti-eNOS (9572S, Cell Signaling Technologies) at 1/500 dilution, rabbit anti-Phospho(Ser1177)-eNOS (9571S, Cell Signaling Technologies) at 1/500 dilution or goat anti-β1 integrin (Santa Cruz, sc-6622) at 1/2,000 dilution as primary antibodies overnight at 4 °C, and HRP-conjugated donkey anti-rabbit (7074, Cell Signaling Technologies) at 1/2,000 dilution and donkey anti-goat (705-035-147, Jackson ImmunoResearch) at 1/5,000 dilution as secondary antibodies for 1 h at RT.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Jackson Immuno peroxidase affinipure donkey anti goat igg
    Peroxidase Affinipure Donkey Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase affinipure donkey anti goat igg/product/Jackson Immuno
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    peroxidase affinipure donkey anti goat igg - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Jackson Immuno peroxidase affinipure goat anti mouse igg
    Peroxidase Affinipure Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 315 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase affinipure goat anti mouse igg/product/Jackson Immuno
    Average 99 stars, based on 315 article reviews
    Price from $9.99 to $1999.99
    peroxidase affinipure goat anti mouse igg - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Jackson Immuno peroxidase affinipure f ab 2 fragment donkey anti goat igg
    Peroxidase Affinipure F Ab 2 Fragment Donkey Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase affinipure f ab 2 fragment donkey anti goat igg/product/Jackson Immuno
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    peroxidase affinipure f ab 2 fragment donkey anti goat igg - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results