atropine each ampoule contains 1 mg of atropine per ml  (Merck KGaA)

 
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    Merck KGaA atropine each ampoule contains 1 mg of atropine per ml
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    ells hmscs 1 × 104 per sample in a 48 well plate  (Millipore)

     
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    Millipore ells hmscs 1 × 104 per sample in a 48 well plate
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    duplicate 0 1 million 100 ml per well  (Bankpeptide biological technology co LTD)

     
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    Bankpeptide biological technology co LTD duplicate 0 1 million 100 ml per well
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    hpgv 1 genome equivalents per milliliter  (Danaher Inc)


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    Danaher Inc hpgv 1 genome equivalents per milliliter
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    hpgv 1 genome equivalents per milliliter  (Danaher Inc)


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    Danaher Inc hpgv 1 genome equivalents per milliliter
    Phylogenetic and selection analysis of the human pegivirus genome population. ( A ) Phylogenetic tree of full-length human pegivirus ORF sequences recovered from 20 HCV patients included in this study and relevant pegivirus reference sequences of genotypes 1–6. The tree is midpoint-rooted, and pegivirus genotypes are indicated at the tips. The scale bar depicts changes per site, and branch labels represent bootstrap support ( n = 100). The prefix letters A, W, and T correspond to the three patient study groups defined in Materials and Methods. ( B ) New synonymous and non-synonymous π (pairwise distance) across the complete pegivirus ORF; the data are averaged for <t>HPgV-1</t> recovered from the 18 out of 20 included HPgV-1/HCV coinfected patients (W101 and W130 were not included since they did not have sufficient coverage to be included, below 5,000 on average as seen in ) in a sliding window of 15 amino acids. A junction of each predicted mature protein is indicated as vertical dotted lines with the protein label shown above. The small space between the translation start and E1 is considered the core region. ( C ) dN/dS ratios of each predicted mature pegivirus protein shown as averages of the HPgV-1 recovered from 18 out of 20 HPgV-1/HCV coinfected HCV patients (W101 and W130 were not included since they did not have sufficient coverage as stated above) with SEM depicted as error bars.
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    1) Product Images from "Effect of direct-acting antivirals on the titers of human pegivirus 1 during treatment of chronic hepatitis C patients"

    Article Title: Effect of direct-acting antivirals on the titers of human pegivirus 1 during treatment of chronic hepatitis C patients

    Journal: Microbiology Spectrum

    doi: 10.1128/spectrum.00641-24

    Phylogenetic and selection analysis of the human pegivirus genome population. ( A ) Phylogenetic tree of full-length human pegivirus ORF sequences recovered from 20 HCV patients included in this study and relevant pegivirus reference sequences of genotypes 1–6. The tree is midpoint-rooted, and pegivirus genotypes are indicated at the tips. The scale bar depicts changes per site, and branch labels represent bootstrap support ( n = 100). The prefix letters A, W, and T correspond to the three patient study groups defined in Materials and Methods. ( B ) New synonymous and non-synonymous π (pairwise distance) across the complete pegivirus ORF; the data are averaged for HPgV-1 recovered from the 18 out of 20 included HPgV-1/HCV coinfected patients (W101 and W130 were not included since they did not have sufficient coverage to be included, below 5,000 on average as seen in ) in a sliding window of 15 amino acids. A junction of each predicted mature protein is indicated as vertical dotted lines with the protein label shown above. The small space between the translation start and E1 is considered the core region. ( C ) dN/dS ratios of each predicted mature pegivirus protein shown as averages of the HPgV-1 recovered from 18 out of 20 HPgV-1/HCV coinfected HCV patients (W101 and W130 were not included since they did not have sufficient coverage as stated above) with SEM depicted as error bars.
    Figure Legend Snippet: Phylogenetic and selection analysis of the human pegivirus genome population. ( A ) Phylogenetic tree of full-length human pegivirus ORF sequences recovered from 20 HCV patients included in this study and relevant pegivirus reference sequences of genotypes 1–6. The tree is midpoint-rooted, and pegivirus genotypes are indicated at the tips. The scale bar depicts changes per site, and branch labels represent bootstrap support ( n = 100). The prefix letters A, W, and T correspond to the three patient study groups defined in Materials and Methods. ( B ) New synonymous and non-synonymous π (pairwise distance) across the complete pegivirus ORF; the data are averaged for HPgV-1 recovered from the 18 out of 20 included HPgV-1/HCV coinfected patients (W101 and W130 were not included since they did not have sufficient coverage to be included, below 5,000 on average as seen in ) in a sliding window of 15 amino acids. A junction of each predicted mature protein is indicated as vertical dotted lines with the protein label shown above. The small space between the translation start and E1 is considered the core region. ( C ) dN/dS ratios of each predicted mature pegivirus protein shown as averages of the HPgV-1 recovered from 18 out of 20 HPgV-1/HCV coinfected HCV patients (W101 and W130 were not included since they did not have sufficient coverage as stated above) with SEM depicted as error bars.

    Techniques Used: Selection

    Human pegivirus viremia development during treatment of HCV with DAA without or with sofosbuvir. ( A ) Individual patient HPgV-1 RNA titer in plasma shown as connected dots with timepoint indicated on the x -axis in SVR HCV patients during GLE/PIB treatment, HCV patients during sofosbuvir-containing treatment referred to as SOF*, including LED/SOF, VEL/SOF, VOX/VEL/SOF, GLE/PIB/SOF, and HCV patients during LED/SOF plus PEG-INF. ( B ) HPgV-1 RNA titer changes in treated patients compared to their respective baseline samples shown as log 10 GE/mL drop on the y -axis and depicted as violin plots. Groupings are identical to (A).
    Figure Legend Snippet: Human pegivirus viremia development during treatment of HCV with DAA without or with sofosbuvir. ( A ) Individual patient HPgV-1 RNA titer in plasma shown as connected dots with timepoint indicated on the x -axis in SVR HCV patients during GLE/PIB treatment, HCV patients during sofosbuvir-containing treatment referred to as SOF*, including LED/SOF, VEL/SOF, VOX/VEL/SOF, GLE/PIB/SOF, and HCV patients during LED/SOF plus PEG-INF. ( B ) HPgV-1 RNA titer changes in treated patients compared to their respective baseline samples shown as log 10 GE/mL drop on the y -axis and depicted as violin plots. Groupings are identical to (A).

    Techniques Used:

    RNA titer decrease of HCV and HPgV-1 in HCV patients during treatment with sofosbuvir-containing regimens. RNA titer measurements in plasma of HCV and HPgV-1 are shown at the y -axis as international units per milliliter (IU/mL) or genome equivalents per milliliter (GE/mL), respectively, and time at the x -axis. The gray boxes indicate periods during antiviral treatment with the type of treatment indicated at the top. Each panel represents a patient treated with regimens containing SOF, including LED/SOF, VEL/SOF, VOX/VEL/SOF, and GLE/PIB/SOF. A red titer line representing HCV RNA at the y -axis represents undetectable HCV RNA titers (limit of detection 15 IU/mL except for patients T134, T167, and T213 with limit of detection 10 IU/mL). Ribavirin addition to the treatment is indicated as RBV. A blue titer line representing HPgV-1 RNA at the y -axis represents undetectable HPgV-1 RNA titers (limit of detection 100 GE/mL).
    Figure Legend Snippet: RNA titer decrease of HCV and HPgV-1 in HCV patients during treatment with sofosbuvir-containing regimens. RNA titer measurements in plasma of HCV and HPgV-1 are shown at the y -axis as international units per milliliter (IU/mL) or genome equivalents per milliliter (GE/mL), respectively, and time at the x -axis. The gray boxes indicate periods during antiviral treatment with the type of treatment indicated at the top. Each panel represents a patient treated with regimens containing SOF, including LED/SOF, VEL/SOF, VOX/VEL/SOF, and GLE/PIB/SOF. A red titer line representing HCV RNA at the y -axis represents undetectable HCV RNA titers (limit of detection 15 IU/mL except for patients T134, T167, and T213 with limit of detection 10 IU/mL). Ribavirin addition to the treatment is indicated as RBV. A blue titer line representing HPgV-1 RNA at the y -axis represents undetectable HPgV-1 RNA titers (limit of detection 100 GE/mL).

    Techniques Used:

    Viremia development of HCV and HPgV-1 in HCV patients during treatment with a sofosbuvir and pegylated interferon-containing regimen. RNA titer measurements in plasma of HCV and HPgV-1 are shown at the y -axis as international units per milliliter (IU/mL) or genome equivalents per milliliter (GE/mL), respectively, and time at the x -axis. The gray boxes indicate periods during antiviral treatment with the type of treatment indicated at the top. Each panel represents a patient treated with LED/SOF plus RBV and PEG-INF. A red titer line representing HCV RNA at the y -axis represents undetectable HCV RNA titers (limit of detection 15 IU/mL). A blue titer line representing HPgV-1 RNA at the y -axis represents undetectable HPgV-1 RNA titers (limit of detection 100 GE/mL).
    Figure Legend Snippet: Viremia development of HCV and HPgV-1 in HCV patients during treatment with a sofosbuvir and pegylated interferon-containing regimen. RNA titer measurements in plasma of HCV and HPgV-1 are shown at the y -axis as international units per milliliter (IU/mL) or genome equivalents per milliliter (GE/mL), respectively, and time at the x -axis. The gray boxes indicate periods during antiviral treatment with the type of treatment indicated at the top. Each panel represents a patient treated with LED/SOF plus RBV and PEG-INF. A red titer line representing HCV RNA at the y -axis represents undetectable HCV RNA titers (limit of detection 15 IU/mL). A blue titer line representing HPgV-1 RNA at the y -axis represents undetectable HPgV-1 RNA titers (limit of detection 100 GE/mL).

    Techniques Used:

    vrna 0 1 µg rna per reaction  (New England Biolabs)


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    New England Biolabs vrna 0 1 µg rna per reaction
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    matrigel 1 mg ml per chamber corning inc  (Corning Life Sciences)

     
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    Corning Life Sciences matrigel 1 mg ml per chamber corning inc
    Matrigel 1 Mg Ml Per Chamber Corning Inc, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    512 pixels spatial resolution 0 1 arcmin of visual angle per pixel  (Xilinx Inc)

     
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    Xilinx Inc 512 pixels spatial resolution 0 1 arcmin of visual angle per pixel
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    plasma starting dilutions of 1 10 22µl in total per well  (Greiner Bio)

     
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    Greiner Bio plasma starting dilutions of 1 10 22µl in total per well
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