Journal: Frontiers in Ophthalmology

Article Title: Time of day differences in the regulation of glutathione levels in the rat lens

doi: 10.3389/fopht.2024.1407582

Figure Lengend Snippet: Primer sequences for target genes.

Article Snippet: BMAL1, PER1, GCLC and β-actin primary antibodies were purchased from Abcam (Cambridge, UK), CLOCK and GR were purchased from ThermoFisher Scientific (Waltham, Massachusetts, USA) and CRY1, CRY2, PER2, PER3, NRF2 and GS were purchased from ProteinTech (Rosemont, Illinois, USA).

Techniques: Sequencing, Amplification

Journal: Frontiers in Ophthalmology

Article Title: Time of day differences in the regulation of glutathione levels in the rat lens

doi: 10.3389/fopht.2024.1407582

Figure Lengend Snippet: Protein expression of clock components of the positive and negative arm of the circadian clock in the rat lens. 20µg/lane of positive control tissue (kidney, liver or brain (n= 1 rat)) and 20µg/lane lens tissue (n=8 rats) was electrophoresed on an SDS-PAGE gel and protein levels analysed by Western blotting. Expression of BMAL1, CLOCK, PER1, PER2, PER3, CRY1 & CRY 2. Arrowheads indicate the predicted size of the target protein. Target proteins were all identified in positive control tissue but not all could be detected in the lens.

Article Snippet: BMAL1, PER1, GCLC and β-actin primary antibodies were purchased from Abcam (Cambridge, UK), CLOCK and GR were purchased from ThermoFisher Scientific (Waltham, Massachusetts, USA) and CRY1, CRY2, PER2, PER3, NRF2 and GS were purchased from ProteinTech (Rosemont, Illinois, USA).

Techniques: Expressing, Positive Control, SDS Page, Western Blot

Journal: Cell Transplantation

Article Title: Olfactory Ensheathing Cell Ameliorate Neuroinflammation Following Spinal Cord Injury Through Upregulating REV-ERBα in Microglia

doi: 10.1177/09636897241261234

Figure Lengend Snippet: A. The rhythmic expression of rev-erbα in control BV2 microglia, LPS treated BV2 microglia, and after co-culture with OEC. B. The rhythmic expression of per 1 in control BV2 microglia, LPS treated BV2 microglia, and after co-culture with OEC. C. The rhythmic expression of per-2 in control BV2 microglia, LPS treated BV2 microglia, and after co-culture with OEC. D. The rhythmic expression of CLOCK in control BV2 microglia, LPS treated BV2 microglia, and after co-culture with OEC. * P < 0.05; ** P < 0.01; * P < 0.05; *** P < 0.001; n = 5. Data are presented as mean ± SD.

Article Snippet: The primary antibodies used in this study are as follow PER 1 (1:1000, a8449, ABclonal, Wuhan, China), PER 2 (1:1000, a3217, ABclonal, Wuhan, China), and REV-ERBα (1:1000, 2129, Cell Signaling, Massachusetts, USA) and GAPDH (1:10000, EPR16884, Abcam, Cambridge, MA).

Techniques: Expressing, Control, Co-Culture Assay

Journal: Cell Transplantation

Article Title: Olfactory Ensheathing Cell Ameliorate Neuroinflammation Following Spinal Cord Injury Through Upregulating REV-ERBα in Microglia

doi: 10.1177/09636897241261234

Figure Lengend Snippet: Circadian gene expression in cortex before and after OEC treatment. (A) The relative expression of REV-ERBα at 7 days post of OEC transplantation. (B) The relative expression of PER 1 at 7 days post of OEC transplantation. (C) The relative expression of PER 2 at 7 days post of OEC transplantation. (D) The relative expression of CLOCK at 7 days post of OEC transplantation. n = 5. Data are presented as mean ± SD. * P < 0.05; ** P < 0.01; * P < 0.05; *** P < 0.001.

Article Snippet: The primary antibodies used in this study are as follow PER 1 (1:1000, a8449, ABclonal, Wuhan, China), PER 2 (1:1000, a3217, ABclonal, Wuhan, China), and REV-ERBα (1:1000, 2129, Cell Signaling, Massachusetts, USA) and GAPDH (1:10000, EPR16884, Abcam, Cambridge, MA).

Techniques: Expressing, Transplantation Assay